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Entry URI http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u15295074i
Entry name Sorensen Anna-Marie et al. 2004 Jul. Plant Cell Physiol. 45(7):905-13.
Title The ABNORMAL GAMETOPHYTES (AGM) gene product of Arabidopsis demonstrates a role in mitosis during gamete development.
Authors Kroeber Sandra|Saedler Heinz|Sorensen Anna-Marie
Abstract Screening a T-DNA mutagenized population of Arabidopsis thaliana for reduced seed set and segregation distortion led to the isolation of the ABNORMAL GAMETOPHYTES (AGM) mutant. Homozygous plants were never recovered, but heterozygous plants showed mitotic defects during gametogenesis resulting in approximately 50% abortion of both the male and female gametes. Isolation of the genomic sequence flanking the co-segregating T-DNA element led to the identification of a gene located on chromosome 5, predicted to encode a transmembrane protein. BLAST homology searches identified two homologous proteins that are not redundant, as is clear from the existence of the agm mutant. Unexpectedly, expression studies using the beta-glucuronidase reporter gene suggest that AGM and its closest Arabidopsis homolog are mostly expressed in cells undergoing mitosis. Thus, AGM is not a gametophytic gene as originally speculated on the basis of segregation distortion, but rather classified as an essential gene crucial to the process of mitosis in plants.
Pubmed ID 15295074
Journal Plant & cell physiology
Volume 45
Issue 7
Pages 905-13
Publication date 2004 Jul
Num of phenotype gene 0