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Entry URI http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u15998312i
Entry name Dellagi Alia et al. 2005 Jul. Plant J. 43(2):262-72.
Title Siderophore-mediated upregulation of Arabidopsis ferritin expression in response to Erwinia chrysanthemi infection.
Authors Briat Jean-Fran巽ois|Cellier Fran巽oise|Dellagi Alia|Expert Dominique|Gaymard Fr辿d辿ric|Kraepiel Yvan|Rigault Martine|Roux Camille|Segond Diego
Abstract Ferritins are multimeric iron storage proteins encoded by a four-member gene family in Arabidopsis (AtFer1-4). To investigate whether iron sequestration in ferritins is a part of an iron-withholding defense system induced in response to bacterial invasion, we used Arabidopsis thaliana as a susceptible host for the pathogenic bacterium Erwinia chrysanthemi. In this study, we used a T-DNA insertion mutant line to show that the lack of a functional AtFer1 gene resulted in an enhanced susceptibility of Arabidopsis plants to E. chrysanthemi. We found that the AtFer1 gene is upregulated during infection, with a biphasic accumulation of the transcript at critical time points 0.5 and 24 h post-infection (p.i.). The activation of AtFer1 expression observed at 24 h p.i. was independent of the iron-dependent regulatory sequence (IDRS) known to mediate the transcriptional response of the AtFer1 gene to iron excess and to nitric oxide. Upregulation of AtFer1 gene expression was compromised after inoculation with an E. chrysanthemi siderophore null mutant. Infiltration of the purified siderophores chrysobactin and desferrioxamine strongly increased AtFer1 transcript abundance and it did not occur with the iron-loaded forms of these siderophores. We found that neither oxidative stress nor nitric oxide was involved in the plant response to chrysobactin. Our data show that ferritin accumulation during infection of Arabidopsis by E. chrysanthemi is a basal defense mechanism which is mainly activated by bacterial siderophores. The potential role of siderophores in this process is discussed.
Pubmed ID 15998312
Journal The Plant journal
Volume 43
Issue 2
Pages 262-72
Publication date 2005 Jul
Num of phenotype gene 0