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| Entry URI | http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u16045475i |
| Entry name | Delvall辿 David et al. 2005 Aug. Plant J. 43(3):398-412. |
| Title | Soluble starch synthase I: a major determinant for the synthesis of amylopectin in Arabidopsis thaliana leaves. |
| Authors | Ball Steven|Berbezy Pierre|Chatterjee Manash|Colonna Paul|D'Hulst Christophe|Delvall辿 David|Dumez Sylvain|M辿rida Angel|Planchot V辿ronique|Rold叩n Isaac|Vyas Darshna|Wattebled Fabrice |
| Abstract | A minimum of four soluble starch synthase families have been documented in all starch-storing green plants. These activities are involved in amylopectin synthesis and are extremely well conserved throughout the plant kingdom. Mutants or transgenic plants defective for SSII and SSIII isoforms have been previously shown to have a large and specific impact on the synthesis of amylopectin while the function of the SSI type of enzymes has remained elusive. We report here that Arabidopsis mutants, lacking a plastidial starch synthase isoform belonging to the SSI family, display a major and novel type of structural alteration within their amylopectin. Comparative analysis of beta-limit dextrins for both wild type and mutant amylopectins suggests a specific and crucial function of SSI during the synthesis of transient starch in Arabidopsis leaves. Considering our own characterization of SSI activity and the previously described kinetic properties of maize SSI, our results suggest that the function of SSI is mainly involved in the synthesis of small outer chains during amylopectin cluster synthesis. |
| Pubmed ID | 16045475 |
| Journal | The Plant journal |
| Volume | 43 |
| Issue | 3 |
| Pages | 398-412 |
| Publication date | 2005 Aug |
| Num of phenotype gene | 0 |