262 |
Entry URI |
http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u16129836i |
Entry name |
Xie Zhixin et al. 2005 Sep. Proc. Natl. Acad. Sci. U.S.A. 102(36):12984-9. |
Title |
DICER-LIKE 4 functions in trans-acting small interfering RNA biogenesis and vegetative phase change in Arabidopsis thaliana. |
Authors |
Allen Edwards|Carrington James C|Wilken April|Xie Zhixin |
Abstract |
Arabidopsis thaliana contains four DICER-LIKE (DCL) genes with specialized functions in small RNA biogenesis for RNA interference-related processes. A mutant with defects in DCL4 was identified and analyzed for microRNA- and endogenous, small interfering RNA (siRNA)-related functions. The dcl4-2 mutant contained normal or near-normal levels of microRNAs (21 nt) and heterochromatin-associated siRNAs (24 nt). In contrast, this mutant lacked each of three families of 21-nt trans-acting siRNAs (ta-siRNAs) and possessed elevated levels of ta-siRNA target transcripts. The dcl4-2 mutant resembled an rna-dependent RNA polymerase 6 mutant in that both mutants lacked ta-siRNAs and displayed heterochronic defects in which vegetative phase change was accelerated. Double mutant analyses with dcl2-1, dcl3-1, and dcl4-2 alleles revealed hierarchical redundancy among DCL activities, leading to alternative processing of ta-siRNA precursors in the absence of DCL4. These data support the concept that plants have specialized and compartmentalized DCL functions for biogenesis of distinct small RNA classes. |
Pubmed ID |
16129836 |
Journal |
Proceedings of the National Academy of Sciences of the United States of America |
Volume |
102 |
Issue |
36 |
Pages |
12984-9 |
Publication date |
2005 Sep |
Num of phenotype gene |
1 |