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Entry URI http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u16129836i
Entry name Xie Zhixin et al. 2005 Sep. Proc. Natl. Acad. Sci. U.S.A. 102(36):12984-9.
Title DICER-LIKE 4 functions in trans-acting small interfering RNA biogenesis and vegetative phase change in Arabidopsis thaliana.
Authors Allen Edwards|Carrington James C|Wilken April|Xie Zhixin
Abstract Arabidopsis thaliana contains four DICER-LIKE (DCL) genes with specialized functions in small RNA biogenesis for RNA interference-related processes. A mutant with defects in DCL4 was identified and analyzed for microRNA- and endogenous, small interfering RNA (siRNA)-related functions. The dcl4-2 mutant contained normal or near-normal levels of microRNAs (21 nt) and heterochromatin-associated siRNAs (24 nt). In contrast, this mutant lacked each of three families of 21-nt trans-acting siRNAs (ta-siRNAs) and possessed elevated levels of ta-siRNA target transcripts. The dcl4-2 mutant resembled an rna-dependent RNA polymerase 6 mutant in that both mutants lacked ta-siRNAs and displayed heterochronic defects in which vegetative phase change was accelerated. Double mutant analyses with dcl2-1, dcl3-1, and dcl4-2 alleles revealed hierarchical redundancy among DCL activities, leading to alternative processing of ta-siRNA precursors in the absence of DCL4. These data support the concept that plants have specialized and compartmentalized DCL functions for biogenesis of distinct small RNA classes.
Pubmed ID 16129836
Journal Proceedings of the National Academy of Sciences of the United States of America
Volume 102
Issue 36
Pages 12984-9
Publication date 2005 Sep
Num of phenotype gene 1