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Entry URI http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u16167900i
Entry name Johnson Kim L et al. 2005 Oct. Plant J. 44(1):114-27.
Title AtDEK1 is essential for specification of embryonic epidermal cell fate.
Authors Degnan Kathryn A|Ingram Gwyneth C|Johnson Kim L|Ross Walker J
Abstract The specification of epidermal (L1) identity occurs early during plant embryogenesis. Here we show that, in Arabidopsis, AtDEK1 encodes a key component of the embryonic L1 cell-layer specification pathway. Loss of AtDEK1 function leads to early embryo lethality characterized by a severe loss of cell organization in the embryo proper and abnormal cell divisions within the suspensor. Markers for L1 identity, ACR4 and ATML1, are not expressed in homozygous mutant embryos. In order to clarify the function of AtDEK1 further, an RNAi knockdown approach was used. This allowed embryos to partially complete embryogenesis before losing AtDEK1 activity. Resulting seedlings showed a specific loss of epidermal cell identity within large portions of the cotyledons. In addition, meristem structure and function was systematically either reduced or entirely lost. AtDEK1 expression is not restricted to the L1 epidermal cell layer at any stage in development. This is consistent with AtDEK1 playing an upstream role in the continuous generation or interpretation of positional information required for epidermal specification. Our results not only identify a specific role for AtDEK1 during embryogenesis, but underline the potential key importance of L1 specification at the globular stage for subsequent progression through embryogenesis.
Pubmed ID 16167900
Journal The Plant journal
Volume 44
Issue 1
Pages 114-27
Publication date 2005 Oct
Num of phenotype gene 0