| 718 | |
| Entry URI | http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u18178771i |
| Entry name | Wen Rui et al. 2008 Jan. Plant Cell 20(1):213-27. |
| Title | Arabidopsis UEV1D promotes Lysine-63-linked polyubiquitination and is involved in DNA damage response. |
| Authors | Lai Xiaoqin|Pastushok Landon|Pelzer Lindsay|Torres-Acosta J Antonio|Wang Hong|Wen Rui|Xiao Wei |
| Abstract | DNA damage tolerance (DDT) in budding yeast requires Lys-63-linked polyubiquitination of the proliferating cell nuclear antigen. The ubiquitin-conjugating enzyme Ubc13 and the Ubc enzyme variant (Uev) methyl methanesulfonate2 (Mms2) are required for this process. Mms2 homologs have been found in all eukaryotic genomes examined; however, their roles in multicellular eukaryotes have not been elucidated. We report the isolation and characterization of four UEV1 genes from Arabidopsis thaliana. All four Uev1 proteins can form a stable complex with At Ubc13 or with Ubc13 from yeast or human and can promote Ubc13-mediated Lys-63 polyubiquitination. All four Uev1 proteins can replace yeast MMS2 DDT functions in vivo. Although these genes are ubiquitously expressed in most tissues, UEV1D appears to express at a much higher level in germinating seeds and in pollen. We obtained and characterized two uev1d null mutant T-DNA insertion lines. Compared with wild-type plants, seeds from uev1d null plants germinated poorly when treated with a DNA-damaging agent. Those that germinated grew slower, and the majority ceased growth within 2 weeks. Pollen from uev1d plants also displayed a moderate but significant decrease in germination in the presence of DNA damage. This report links Ubc13-Uev with functions in DNA damage response in Arabidopsis. |
| Pubmed ID | 18178771 |
| Journal | The Plant cell |
| Volume | 20 |
| Issue | 1 |
| Pages | 213-27 |
| Publication date | 2008 Jan |
| Num of phenotype gene | 0 |