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Entry URI http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u18218967i
Entry name Van der Ent Sjoerd et al. 2008 Mar. Plant Physiol. 146(3):1293-304.
Title MYB72 is required in early signaling steps of rhizobacteria-induced systemic resistance in Arabidopsis.
Authors Bakker Daniel|Joosten Ruth G|Pel Michiel J C|Pieterse Corné M J|Proveniers Marcel C G|Ton Jurriaan|Van Doorn Ronald|Van Loon L C|Van der Ent Sjoerd|Verhagen Bas W M|Verlaan Maarten G
Abstract Colonization of Arabidopsis thaliana roots by nonpathogenic Pseudomonas fluorescens WCS417r bacteria triggers a jasmonate/ethylene-dependent induced systemic resistance (ISR) that is effective against a broad range of pathogens. Microarray analysis revealed that the R2R3-MYB-like transcription factor gene MYB72 is specifically activated in the roots upon colonization by WCS417r. Here, we show that T-DNA knockout mutants myb72-1 and myb72-2 are incapable of mounting ISR against the pathogens Pseudomonas syringae pv tomato, Hyaloperonospora parasitica, Alternaria brassicicola, and Botrytis cinerea, indicating that MYB72 is essential to establish broad-spectrum ISR. Overexpression of MYB72 did not result in enhanced resistance against any of the pathogens tested, demonstrating that MYB72 is not sufficient for the expression of ISR. Yeast two-hybrid analysis revealed that MYB72 physically interacts in vitro with the ETHYLENE INSENSITIVE3 (EIN3)-LIKE3 transcription factor EIL3, linking MYB72 function to the ethylene response pathway. However, WCS417r activated MYB72 in ISR-deficient, ethylene-insensitive ein2-1 plants. Moreover, exogenous application of the ethylene precursor 1-aminocyclopropane-1-carboxylate induced wild-type levels of resistance in myb72-1, suggesting that MYB72 acts upstream of ethylene in the ISR pathway. Collectively, this study identified the transcriptional regulator MYB72 as a novel ISR signaling component that is required in the roots during early signaling steps of rhizobacteria-mediated ISR.
Pubmed ID 18218967
Journal Plant physiology
Volume 146
Issue 3
Pages 1293-304
Publication date 2008 Mar
Num of phenotype gene 0