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Entry URI http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u15053758i
Entry name Baba Kyoko et al. 2004 Apr. Plant J. 38(1):38-48.
Title Organellar gene transcription and early seedling development are affected in the rpoT;2 mutant of Arabidopsis.
Authors Baba Kyoko|Bhalerao Rishikesh P|Espinosa-Ruiz Ana|Gardestr旦m Per|Sane Aniruddha P|Schmidt Julien|Shiina Takashi|Villarejo Arsenio
Abstract An Arabidopsis mutant that exhibited reduced root length was isolated from a population of activation-tagged T-DNA insertion lines in a screen for aberrant root growth. This mutant also exhibited reduced hypocotyl length as well as a delay in greening and altered leaf shape. Molecular genetic analysis of the mutant indicated a single T-DNA insertion in the gene RpoT;2 encoding a homolog of the phage-type RNA polymerase (RNAP), that is targeted to both mitochondria and plastids. A second T-DNA-tagged allele also showed a similar phenotype. The mutation in RpoT;2 affected the light-induced accumulation of several plastid mRNAs and proteins and resulted in a lower photosynthetic efficiency. In contrast to the alterations in the plastid gene expression, no major effect of the rpoT;2 mutation on the accumulation of examined mitochondrial gene transcripts and proteins was observed. The rpoT;2 mutant exhibited tissue-specific alterations in the transcript levels of two other organelle-directed nuclear-encoded RNAPs, RpoT;1 and RpoT;3. This suggests the existence of cross-talk between the regulatory pathways of the three RNAPs through organelle to nucleus communication. These data provide an important information on a role of RpoT;2 in plastid gene expression and early plant development.
Pubmed ID 15053758
Journal The Plant journal
Volume 38
Issue 1
Pages 38-48
Publication date 2004 Apr
Num of phenotype gene 0