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| Entry URI | http://metadb.riken.jp/db/SciNetS_ria224i/cria224u4ria224u15197266i |
| Entry name | Schi淡tt Morten et al. 2004 Jun. Proc. Natl. Acad. Sci. U.S.A. 101(25):9502-7. |
| Title | A plant plasma membrane Ca2+ pump is required for normal pollen tube growth and fertilization. |
| Authors | Baekgaard Lone|Harper Jeffrey F|Jakobsen Mia Kyed|Palmgren Michael G|Romanowsky Shawn M|Schi淡tt Morten |
| Abstract | Ca(2+) signals are thought to play important roles in plant growth and development, including key aspects of pollen tube growth and fertilization. The dynamics of a Ca(2+) signal are largely controlled by influx (through channels) and efflux (through pumps and antiporters). The Arabidopsis genome encodes 14 Ca(2+) pumps, 10 of which belong to a family of autoinhibited Ca(2+) ATPases (ACA) that are predicted to be activated by Ca(2+)/calmodulin. Here, we show that isoform ACA9 is expressed primarily in pollen and localized to the plasma membrane. Three independent T-DNA [portion of the Ti (tumor-inducing) plasmid that is transferred to plant cells] gene disruptions of ACA9 were found to result in partial male sterility. Complementation was observed by using a ACA9-yellow fluorescence protein (YFP) fusion that displayed plasma membrane localization. Mutant aca9 pollen displayed a reduced growth potential and a high frequency of aborted fertilization, resulting in a >80% reduction in seed set. These findings identify a plasma membrane Ca(2+) transporter as a key regulator of pollen development and fertilization in flowering plants. |
| Pubmed ID | 15197266 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 101 |
| Issue | 25 |
| Pages | 9502-7 |
| Publication date | 2004 Jun |
| Num of phenotype gene | 0 |