>AHC444 (AHC444Q) /CSM/AH/AHC4-B/AHC444Q.Seq.d/
ATTAATTTTAATTTATATAAATACCTTATTACTTAAAGATGACAACAAATAATCCATTTG
ATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAA
AACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAG
CAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATT
GGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAG
ATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGAT
TAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACT
CTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAAT
TCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGAT
TATTTATTGCTCCAXXXXXXXXXXATCTTGGTGATAGTATTACCACTGATCATATTTCAC
CAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAA
AACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAG
GTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAA
CCTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAG
AAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATT
GGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAAC
GTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTC
AAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTG
ATAAATCCCTCATTAAAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTT
TTGAAACTATCCTTCGTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTA
TCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTTCTTTTTTAATATAAAATAAAAA
AAAATAATATTAAAAAAACAAAAA

*f*fi*IPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLESA
VRNCDNFEVHEKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKRL
GGDPAKINPLVPVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDGL
FIAP---

---LGDSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANT
RIVNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGP
YLQGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIK
TGQTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flf*ykikknnikk
tk

Frame A:
infnlykylit*r*qqiihlik*rmy*nhkikliifiifqnfktqelknyhtpleffwnq
qfvivitlkytkkmlkis*igktlqiklkflsnqqeyyykillvyqqllilqq*emp*kd
*vviqqklthlfqli*llitlfklmyqellml*skikrlnsieimkdfhf*ngvlkhlmd
ylll---

---ilvivlpliifhqpvtsienhqplvi*spkv*nqkiltpmdlvevmmklwlevplpi
lvllinwlqplvhkqpmyqlvs*clfqmlqkninqkvinslfwlvvimalvvleiglpkv
htfkelsvslpshlnvfidpislvwvlshcnsnqvkmpllwvslvknnsissyqlinpsl
klvkp*rlplivvnllklsfvsilqsklnigqimvsylmf*enfyinfffnik*kkiilk
kqk

Frame B:
liliyintlllkddnk*si**skgcikitrsnl*fl*sfktsrpkn*kitilh*nsfgis
ss*l**l*strkrc*kylklgkhck*s*nsfqtsksiitrfywctssc*scsnercheki
rw*sskn*ptcss*fsy*slcss*ciknc*cfrakskd*ip*ks*kififkmgcssi*wi
iycs---

---sw**yyh*syftsr*hq*kiisrslfrvqrcktrrf*hlwis*r***nyg*rylcqy
syc**igssrwstnnlctnw*vnvyfrccrkisirrssthcfgw**lwlw*f*rlgcqrs
ipsrn*vchchli*tys*iqsrwygyypiaiptrskclyfgshw*rtiqyrvtn**iph*
nwsnrkgyh*lw*if*nypsfrysnrs*ilgk*wylilcfkktftlisfli*nkkk*y*k
nk

Frame C:
*f*fi*IPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLESA
VRNCDNFEVHEKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKRL
GGDPAKINPLVPVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDGL
FIAP---

---LGDSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANT
RIVNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGP
YLQGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIK
TGQTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flf*ykikknnikk
tk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AHC444 (AHC444Q) /CSM/AH/AHC4-B/AHC444Q.Seq.d/                       2416   0.0
VHA182 (VHA182Q) /CSM/VH/VHA1-D/VHA182Q.Seq.d/                       1358   0.0
SHA509 (SHA509Q) /CSM/SH/SHA5-A/SHA509Q.Seq.d/                       1358   0.0
CHQ778 (CHQ778Q) /CSM/CH/CHQ7-D/CHQ778Q.Seq.d/                       1358   0.0
CHL182 (CHL182Q) /CSM/CH/CHL1-D/CHL182Q.Seq.d/                       1358   0.0
AHC891 (AHC891Q) /CSM/AH/AHC8-D/AHC891Q.Seq.d/                       1358   0.0
VHA161 (VHA161Q) /CSM/VH/VHA1-C/VHA161Q.Seq.d/                       1356   0.0
AHF254 (AHF254Q) /CSM/AH/AHF2-C/AHF254Q.Seq.d/                       1356   0.0
VHE337 (VHE337Q) /CSM/VH/VHE3-B/VHE337Q.Seq.d/                       1354   0.0
SHG221 (SHG221Q) /CSM/SH/SHG2-A/SHG221Q.Seq.d/                       1354   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF194945|AF194945.1 Nicotiana tabacum cytosolic aconitase (ACO) mRNA, complete cds.    66   4e-34  6
AY250116|AY250116.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   3e-26  5
AY250115|AY250115.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   1e-21  5
BE579347|BE579347.1 kq26c09.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:ZK455.1 CE03812 PROBABLE ACONITATE HYDRATASE, CYTOPLASMIC ;, mRNA sequence.    64   1e-20  3
BI925204|BI925204.1 EST545093 tomato flower, buds 0-3 mm Lycopersicon esculentum cDNA clone cTOA25H12 5' end, mRNA sequence.    58   4e-19  3
BI930520|BI930520.1 EST550409 tomato flower, 8 mm to preanthesis buds Lycopersicon esculentum cDNA clone cTOC16J14 5' end, mRNA sequence.    58   4e-19  3
CK265172|CK265172.1 EST711250 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POABX20 5' end, mRNA sequence.    58   7e-18  4
AY062772|AY062772.1 Arabidopsis thaliana Unknown protein (At4g35830; F4B14.100) mRNA, complete cds.    46   3e-17  6
BQ511380|BQ511380.2 EST618795 Generation of a set of potato cDNA clones for microarray analyses mixed potato tissues Solanum tuberosum cDNA clone STMHR19 5' end, mRNA sequence.    58   5e-16  3
D29629|D29629.1 Cucurbita cv. Kurokawa Amakuri mRNA for aconitase, complete cds.    54   8e-16  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   447   e-124
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   294   5e-78
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   292   1e-77
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   291   3e-77
BT060820_1(BT060820|pid:none) Zea mays full-length cDNA clone ZM...   290   6e-77
BT060665_1(BT060665|pid:none) Zea mays full-length cDNA clone ZM...   290   9e-77
DQ294230_1(DQ294230|pid:none) Glossina morsitans morsitans TC165...   287   5e-76
(P49608) RecName: Full=Aconitate hydratase, cytoplasmic;        ...   286   8e-76
(Q9SIB9) RecName: Full=Aconitate hydratase 2, mitochondrial;    ...   285   2e-75
BC168529_1(BC168529|pid:none) Xenopus tropicalis cDNA clone MGC:...   285   3e-75

 psg: 0.66  gvh: 0.34  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AHC444 is cyt

>AHC444F.Seq
ATTAATTTTAATTTATATAAATACCTTATTACTTAAAGATGACAACAAATAATCCATTTG
ATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAA
AACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAG
CAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATT
GGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAG
ATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGAT
TAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACT
CTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAAT
TCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGAT
TATTTATTGCTCCANNNNNNNNNN

>AHC444Z.Seq
NNNNNNNNNNATCTTGGTGATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATC
AATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTT
AACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAAT
ACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACT
GGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTC
ATTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGT
CCATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCC
AATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACT
TTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATT
AAAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTT
CGTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTT
TTAAGAAAACTTTTACATTAATTTCTTTTTTAATATAAAATAAAAAAAAATAATATTAAA
AAAACAAAAA

>AHC444P.Seq
ATTAATTTTAATTTATATAAATACCTTATTACTTAAAGATGACAACAAATAATCCATTTG
ATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAA
AACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAG
CAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATT
GGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAG
ATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGAT
TAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACT
CTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAAT
TCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGAT
TATTTATTGCTCCA----------ATCTTGGTGATAGTATTACCACTGATCATATTTCAC
CAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAA
AACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAG
GTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAA
CCTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAG
AAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATT
GGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAAC
GTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTC
AAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTG
ATAAATCCCTCATTAAAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTT
TTGAAACTATCCTTCGTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTA
TCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTTCTTTTTTAATATAAAATAAAAA
AAAATAATATTAAAAAAACAAAAA