>AFC302 (AFC302Q) /CSM/AF/AFC3-A/AFC302Q.Seq.d/
ATTTGTAAATATAAAAAAAAATGGAATCATTAAAAAACAATGGTGAAAAAATTGAAAATT
TTTTAAATCAATTAAAAAATCTTCAATCAAATGTAGACAAAGATTTTAAAGAATTTTCAA
ATTCTCCAAATACTTTAAATGCTGAAAAAGATGGAGAGTTTCTTGATAAACATGATGAAG
AATTATACAACAAACAAATAATTCGTTCAAGACTAGAAGATAAAATTTCAAATTTAAGTT
CTTCTATGATTAATGATATTGGGAATGATTTTCAAACTGGTTTTCAAACAATTCTATACA
ATTTACCAGGTGTTGGTCCAGTTTTCCAAATGATCTTTAATATTATGGGTAATGGTATAA
ATTTAGAAAAATTTTACCAAGAAATCATTACACAAGTAGAACAAATGATTAAAAAATCAT
TAGAAGATTATTATAAAAACCAATGTAATACAATTTTTAAAAATTTAGGAAAAGCATGTG
ATCAACATAAAGATCTAACTCAAAAATGGTATGATAAAAATGGTATCAAATCAATGAACC
ATTTAGGTAAAGAAATTCCACAATCAACATCATCCACAATTGAATCTGATGATACAXXXX
XXXXXXAAGGTTTGGGTTCCACCACAACAAGCATCGTGGGTATCTCCTCCTGCCTCGGAA
TTGGCACATAAATTCTTTTTGGCCCATTCTGACAATTATCCTCAAGGTGGTGATCTAATT
TATATTAAAAAAGATGGAGTTTATGAACTTAGTCCAAATAAAATAACTCATGCCCTTGAA
GTTTATTATAGCTCTGGGGGTGGATATGGTGTTAATGACTATCCAAGTTTTCGTTATGGT
GGAAAACCAATCATACCTCTTCTCGCTCCAGTTGGTAATGCTGTGTTTACAATGTTAAAT
CCAAATCGTTATAAAAGAACCTTTAAAATTAGAATTGTTCATGTCATTGTTAGAGAAGCC
AAATGGAATTTAGATTGCTATGATAATCAAGTTGGGGAAGCAGGTTCATTTAACCAAAAT
TTTGTTTTCACTTCAACTAATATAGTTTCTGACCCAATTTGTGGGAATATTGGAACTGTT
GGTATCACTGAAATTCCAGGACCATTTACAACTGATAAAAAGTATATTAGATTAACATGC
ATTCGTAAAGTAAATTTCCCAGCATCAAAAAATGAAGGATATGCCGTTGGATCTCGTATT
TTATCAGTACAATTAATTATTTATAACCACTTCAATTAAAATNATTTTCATTTAAAATAT
TG

l*i*KKMESLKNNGEKIENFLNQLKNLQSNVDKDFKEFSNSPNTLNAEKDGEFLDKHDEE
LYNKQIIRSRLEDKISNLSSSMINDIGNDFQTGFQTILYNLPGVGPVFQMIFNIMGNGIN
LEKFYQEIITQVEQMIKKSLEDYYKNQCNTIFKNLGKACDQHKDLTQKWYDKNGIKSMNH
LGKEIPQSTSSTIESDDT---

---KVWVPPQQASWVSPPASELAHKFFLAHSDNYPQGGDLIYIKKDGVYELSPNKITHAL
EVYYSSGGGYGVNDYPSFRYGGKPIIPLLAPVGNAVFTMLNPNRYKRTFKIRIVHVIVRE
AKWNLDCYDNQVGEAGSFNQNFVFTSTNIVSDPICGNIGTVGITEIPGPFTTDKKYIRLT
CIRKVNFPASKNEGYAVGSRILSVQLIIYNHFN*nxfhlky

Frame A:
ickykkkwnh*ktmvkklkif*in*kifnqm*tkilknfqilqil*mlkkmesflinmmk
nyttnk*fvqd*kikfqi*vll*lmilgmifklvfkqfytiyqvlvqfsk*slilwvmv*
i*knftkkslhk*nk*lknh*kiiiktnviqflki*ekhviniki*lkngmikmvsnq*t
i*vkkfhnqhhpqlnlmi---

---KVWVPPQQASWVSPPASELAHKFFLAHSDNYPQGGDLIYIKKDGVYELSPNKITHAL
EVYYSSGGGYGVNDYPSFRYGGKPIIPLLAPVGNAVFTMLNPNRYKRTFKIRIVHVIVRE
AKWNLDCYDNQVGEAGSFNQNFVFTSTNIVSDPICGNIGTVGITEIPGPFTTDKKYIRLT
CIRKVNFPASKNEGYAVGSRILSVQLIIYNHFN*nxfhlky

Frame B:
fvnikkngiikkqw*kn*kffksikkssikcrqrf*rifkfskyfkc*krwrvs**t**r
iiqqtnnsfktrr*nfkfkffyd**ywe*fsnwfsnnsiqftrcwssfpndl*yyg*wyk
frkilprnhytsrtnd*kiirrll*kpm*ynf*kfrksm*st*rsnskmv**kwyqinep
fr*rnstiniihn*i**y---

---rfgfhhnkhrgylllprnwhinsfwpiltiilkvvi*filkkmefmnlvqik*lmpl
kfiialgvdmvlmtiqvfvmvenqsylfslqlvmlclqc*iqivikeplklelfmsllek
pngi*iamiiklgkqvhltkilfslqli*fltqfvgilellvslkfqdhlqliksild*h
afvk*isqhqkmkdmpldlvfyqyn*lfittsikxifi*ni

Frame C:
l*i*KKMESLKNNGEKIENFLNQLKNLQSNVDKDFKEFSNSPNTLNAEKDGEFLDKHDEE
LYNKQIIRSRLEDKISNLSSSMINDIGNDFQTGFQTILYNLPGVGPVFQMIFNIMGNGIN
LEKFYQEIITQVEQMIKKSLEDYYKNQCNTIFKNLGKACDQHKDLTQKWYDKNGIKSMNH
LGKEIPQSTSSTIESDDT---

---glgstttsivgissclgigt*ilfgpf*qlssrw*snly*krwsl*t*sk*nnscp*
sll*lwgwiwc**lskfslwwktnhtssrssw*ccvynvksksl*knl*n*ncschc*rs
qmefrll**sswgsrfi*pkfcfhfn*ysf*pnlweywncwyh*nsrtiyn**kvy*inm
hs*skfpsikk*ricrwisyfistinyl*plqlkxfsfkil

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFC302 (AFC302Q) /CSM/AF/AFC3-A/AFC302Q.Seq.d/                       2254   0.0
AFN784 (AFN784Q) /CSM/AF/AFN7-D/AFN784Q.Seq.d/                       1294   0.0
AFM788 (AFM788Q) /CSM/AF/AFM7-D/AFM788Q.Seq.d/                       1294   0.0
AFI831 (AFI831Q) /CSM/AF/AFI8-B/AFI831Q.Seq.d/                       1294   0.0
AFH880 (AFH880Q) /CSM/AF/AFH8-D/AFH880Q.Seq.d/                       1294   0.0
AFG364 (AFG364Q) /CSM/AF/AFG3-C/AFG364Q.Seq.d/                       1294   0.0
AFH740 (AFH740Q) /CSM/AF/AFH7-B/AFH740Q.Seq.d/                       1292   0.0
CFC285 (CFC285Q) /CSM/CF/CFC2-D/CFC285Q.Seq.d/                       1289   0.0
AFN662 (AFN662Q) /CSM/AF/AFN6-C/AFN662Q.Seq.d/                       1289   0.0
AFN247 (AFN247Q) /CSM/AF/AFN2-B/AFN247Q.Seq.d/                       1289   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116920|AC116920.2 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.  1211  0.0  9
M15968|M15968.1 D.discoideum M3R gene, exons 1-3.                549  0.0  3
M15967|M15967.1 D.discoideum M3L gene, exons 1-3.                268  e-160  4
AL139077|AL139077.2 Campylobacter jejuni NCTC11168 complete genome; segment 4/6.    38  0.005  12
BZ890913|BZ890913.1 CH240_200D14.TJ CHORI-240 Bos taurus genomic clone CH240_200D14, DNA sequence.    52  0.017  1
AC017114|AC017114.4 Homo sapiens chromosome 5 clone RP11-578D7, WORKING DRAFT SEQUENCE, 25 unordered pieces.    36  0.018  8
AC109361|AC109361.4 Homo sapiens BAC clone RP11-710F7 from 4, complete sequence.    38  0.019  4
AJ010592|AJ010592.2 Guillardia theta DNA for complete sequence of nucleomorph chromosome 2.    34  0.025  12
AL445563|AL445563.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 1/3.    32  0.047  14
AC026100|AC026100.19 Homo sapiens 3 BAC RP11-449F7 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    32  0.078  12

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P11872) RecName: Full=cAMP-regulated M3R protein; AltName: Full...   402   e-110
AC117072_17(AC117072|pid:none) Dictyostelium discoideum chromoso...   279   1e-73
AE014187_536(AE014187|pid:none) Plasmodium falciparum 3D7 chromo...    48   8e-04
EF158087_1(EF158087|pid:none) Plasmodium falciparum erythrocyte ...    44   0.009
CR380952_40(CR380952|pid:none) Candida glabrata strain CBS138 ch...    42   0.033
CP000879_149(CP000879|pid:none) Petrotoga mobilis SJ95, complete...    40   0.17
(O51267) RecName: Full=Leucyl-tRNA synthetase;          EC=6.1.1...    40   0.22
CP001205_240(CP001205|pid:none) Borrelia burgdorferi ZS7, comple...    40   0.22
AF128451_1(AF128451|pid:none) Borrelia turicatae plasmid-encoded...    40   0.22
AC116920_30(AC116920|pid:none) Dictyostelium discoideum chromoso...    39   0.28

 psg: 0.54  gvh: 0.29  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: endoplasmic reticulum

>> prediction for AFC302 is cyt

>AFC302F.Seq
ATTTGTAAATATAAAAAAAAATGGAATCATTAAAAAACAATGGTGAAAAAATTGAAAATT
TTTTAAATCAATTAAAAAATCTTCAATCAAATGTAGACAAAGATTTTAAAGAATTTTCAA
ATTCTCCAAATACTTTAAATGCTGAAAAAGATGGAGAGTTTCTTGATAAACATGATGAAG
AATTATACAACAAACAAATAATTCGTTCAAGACTAGAAGATAAAATTTCAAATTTAAGTT
CTTCTATGATTAATGATATTGGGAATGATTTTCAAACTGGTTTTCAAACAATTCTATACA
ATTTACCAGGTGTTGGTCCAGTTTTCCAAATGATCTTTAATATTATGGGTAATGGTATAA
ATTTAGAAAAATTTTACCAAGAAATCATTACACAAGTAGAACAAATGATTAAAAAATCAT
TAGAAGATTATTATAAAAACCAATGTAATACAATTTTTAAAAATTTAGGAAAAGCATGTG
ATCAACATAAAGATCTAACTCAAAAATGGTATGATAAAAATGGTATCAAATCAATGAACC
ATTTAGGTAAAGAAATTCCACAATCAACATCATCCACAATTGAATCTGATGATACA----
------

>AFC302Z.Seq
----------AAGGTTTGGGTTCCACCACAACAAGCATCGTGGGTATCTCCTCCTGCCTC
GGAATTGGCACATAAATTCTTTTTGGCCCATTCTGACAATTATCCTCAAGGTGGTGATCT
AATTTATATTAAAAAAGATGGAGTTTATGAACTTAGTCCAAATAAAATAACTCATGCCCT
TGAAGTTTATTATAGCTCTGGGGGTGGATATGGTGTTAATGACTATCCAAGTTTTCGTTA
TGGTGGAAAACCAATCATACCTCTTCTCGCTCCAGTTGGTAATGCTGTGTTTACAATGTT
AAATCCAAATCGTTATAAAAGAACCTTTAAAATTAGAATTGTTCATGTCATTGTTAGAGA
AGCCAAATGGAATTTAGATTGCTATGATAATCAAGTTGGGGAAGCAGGTTCATTTAACCA
AAATTTTGTTTTCACTTCAACTAATATAGTTTCTGACCCAATTTGTGGGAATATTGGAAC
TGTTGGTATCACTGAAATTCCAGGACCATTTACAACTGATAAAAAGTATATTAGATTAAC
ATGCATTCGTAAAGTAAATTTCCCAGCATCAAAAAATGAAGGATATGCCGTTGGATCTCG
TATTTTATCAGTACAATTAATTATTTATAACCACTTCAATTAAAATNATTTTCATTTAAA
ATATTG

>AFC302P.Seq
ATTTGTAAATATAAAAAAAAATGGAATCATTAAAAAACAATGGTGAAAAAATTGAAAATT
TTTTAAATCAATTAAAAAATCTTCAATCAAATGTAGACAAAGATTTTAAAGAATTTTCAA
ATTCTCCAAATACTTTAAATGCTGAAAAAGATGGAGAGTTTCTTGATAAACATGATGAAG
AATTATACAACAAACAAATAATTCGTTCAAGACTAGAAGATAAAATTTCAAATTTAAGTT
CTTCTATGATTAATGATATTGGGAATGATTTTCAAACTGGTTTTCAAACAATTCTATACA
ATTTACCAGGTGTTGGTCCAGTTTTCCAAATGATCTTTAATATTATGGGTAATGGTATAA
ATTTAGAAAAATTTTACCAAGAAATCATTACACAAGTAGAACAAATGATTAAAAAATCAT
TAGAAGATTATTATAAAAACCAATGTAATACAATTTTTAAAAATTTAGGAAAAGCATGTG
ATCAACATAAAGATCTAACTCAAAAATGGTATGATAAAAATGGTATCAAATCAATGAACC
ATTTAGGTAAAGAAATTCCACAATCAACATCATCCACAATTGAATCTGATGATACA----
------AAGGTTTGGGTTCCACCACAACAAGCATCGTGGGTATCTCCTCCTGCCTCGGAA
TTGGCACATAAATTCTTTTTGGCCCATTCTGACAATTATCCTCAAGGTGGTGATCTAATT
TATATTAAAAAAGATGGAGTTTATGAACTTAGTCCAAATAAAATAACTCATGCCCTTGAA
GTTTATTATAGCTCTGGGGGTGGATATGGTGTTAATGACTATCCAAGTTTTCGTTATGGT
GGAAAACCAATCATACCTCTTCTCGCTCCAGTTGGTAATGCTGTGTTTACAATGTTAAAT
CCAAATCGTTATAAAAGAACCTTTAAAATTAGAATTGTTCATGTCATTGTTAGAGAAGCC
AAATGGAATTTAGATTGCTATGATAATCAAGTTGGGGAAGCAGGTTCATTTAACCAAAAT
TTTGTTTTCACTTCAACTAATATAGTTTCTGACCCAATTTGTGGGAATATTGGAACTGTT
GGTATCACTGAAATTCCAGGACCATTTACAACTGATAAAAAGTATATTAGATTAACATGC
ATTCGTAAAGTAAATTTCCCAGCATCAAAAAATGAAGGATATGCCGTTGGATCTCGTATT
TTATCAGTACAATTAATTATTTATAACCACTTCAATTAAAATNATTTTCATTTAAAATAT
TG