>AFC338 (AFC338Q) /CSM/AF/AFC3-B/AFC338Q.Seq.d/
XXXXXXXXXXGATGAAATAAAATNTGATGATGATTTTGATAAAAGAATTGAATCAGGTAA
NCAAGCTGAAAAGTCAAAGAATAAAGAAAAAAAGAAACATCATTCTCACCATCACTCTCA
CACTAGTAAAGATGGTCGTAGTAGAACAAGTACAAGTACAAGTATTTCAGGTAATGGTCA
TGCAACTGCTTCTGCCTCTGCTTCTTCTTCTTCTTCTTCTACTTCTTTCTCTCATTCTTC
CTCTTCAAATGATCATCACCATAAAAAGGATAAATCAAATAAAAACAAAGACAACAAAGA
AAAAGGTGATAAAAAAGATAAAAAAGATAAAAAAGAAAAGAAAGATAAGAAAAAAGATAA
AAAAGATAAATCAACCTCTGTAGAAGAAGAAATCAATACCTTAAAAGTCGAAAAGAATTC
AATTTAAAATTT

---EIKXDDDFDKRIESGXQAEKSKNKEKKKHHSHHHSHTSKDGRSRTSTSTSISGNGHA
TASASASSSSSSTSFSHSSSSNDHHHKKDKSNKNKDNKEKGDKKDKKDKKEKKDKKKDKK
DKSTSVEEEINTLKVEKNSI*n

Frame A:
---mk*nxmmilikelnqvxklksqrikkkrniiltitltlvkmvvveqvqvqvfqvmvm
qlllpllllllllllslilplqmiitikrinqiktkttkkkvikkikkikkkrkirkkik
kinqpl*kkksip*kskriqfki


Frame B:
---*nkx***f**kn*ir*xs*kvke*rkketsfspslsh**rws**nkykykyfr*wsc
ncfclcffffffyfflsfflfk*ssp*kg*ik*kqrqqrkr**kr*kr*krker*ekr*k
r*inlcrrrnqylksrkefnlkf


Frame C:
---EIKXDDDFDKRIESGXQAEKSKNKEKKKHHSHHHSHTSKDGRSRTSTSTSISGNGHA
TASASASSSSSSTSFSHSSSSNDHHHKKDKSNKNKDNKEKGDKKDKKDKKEKKDKKKDKK
DKSTSVEEEINTLKVEKNSI*n

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH413 (VSH413Q) /CSM/VS/VSH4-A/VSH413Q.Seq.d/                        299   2e-80
VSA102 (VSA102Q) /CSM/VS/VSA1-A/VSA102Q.Seq.d/                        299   2e-80
VFH877 (VFH877Q) /CSM/VF/VFH8-D/VFH877Q.Seq.d/                        299   2e-80
VFB765 (VFB765Q) /CSM/VF/VFB7-C/VFB765Q.Seq.d/                        299   2e-80
SFJ787 (SFJ787Q) /CSM/SF/SFJ7-D/SFJ787Q.Seq.d/                        299   2e-80
SFJ362 (SFJ362Q) /CSM/SF/SFJ3-C/SFJ362Q.Seq.d/                        299   2e-80
CHE494 (CHE494Q) /CSM/CH/CHE4-D/CHE494Q.Seq.d/                        299   2e-80
CFH108 (CFH108Q) /CSM/CF/CFH1-A/CFH108Q.Seq.d/                        299   2e-80
CFA364 (CFA364Q) /CSM/CF/CFA3-C/CFA364Q.Seq.d/                        299   2e-80
AFL225 (AFL225Q) /CSM/AF/AFL2-B/AFL225Q.Seq.d/                        299   2e-80

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL413220|AL413220.1 T7 end of clone AW0AA023A12 of library AW0AA from strain CLIB 89 of Yarrowia lipolytica.    44  1.4  1
AL393354|AL393354.1 T3 end of clone AR0AA010D12 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    44  1.4  1
AQ891478|AQ891478.1 HS_3081_B2_B09_T7C CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=3081 Col=18 Row=D, genomic survey sequence.    40  3.4  2
AL051942|AL051942.1 Drosophila melanogaster genome survey sequence T7 end of BAC #  BACR02A07 of RPCI-98 library from Drosophila melanogaster (fruit  fly).    36  4.5  2
AL411316|AL411316.1 T3 end of clone AW0AA010C07 of library AW0AA from strain CLIB 89 of Yarrowia lipolytica.    42  5.4  1
AL411063|AL411063.1 T7 end of clone AW0AA008G04 of library AW0AA from strain CLIB 89 of Yarrowia lipolytica.    42  5.4  1
BQ864927|BQ864927.1 QGC28B20.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC28B20, mRNA sequence.    42  5.4  1
AL411315|AL411315.1 T7 end of clone AW0AA010C07 of library AW0AA from strain CLIB 89 of Yarrowia lipolytica.    42  5.4  1
BM914240|BM914240.1 AGENCOURT_6608060 NIH_MGC_98 Homo sapiens cDNA clone IMAGE:5479128 5', mRNA sequence.    42  5.4  1
BH382597|BH382597.1 AG-ND-160N23.TR ND-TAM Anopheles gambiae genomic clone AG-ND-160N23 , DNA sequence.    42  5.4  1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 133,157,081

 psg: 0.75  gvh: 0.32  alm: 0.60  top: 0.53  tms: 0.00  mit: 0.10  mip: 0.00
 nuc: 0.36  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	 12.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for AFC338 is nuc

>AFC338Z.Seq
----------GATGAAATAAAATNTGATGATGATTTTGATAAAAGAATTGAATCAGGTAA
NCAAGCTGAAAAGTCAAAGAATAAAGAAAAAAAGAAACATCATTCTCACCATCACTCTCA
CACTAGTAAAGATGGTCGTAGTAGAACAAGTACAAGTACAAGTATTTCAGGTAATGGTCA
TGCAACTGCTTCTGCCTCTGCTTCTTCTTCTTCTTCTTCTACTTCTTTCTCTCATTCTTC
CTCTTCAAATGATCATCACCATAAAAAGGATAAATCAAATAAAAACAAAGACAACAAAGA
AAAAGGTGATAAAAAAGATAAAAAAGATAAAAAAGAAAAGAAAGATAAGAAAAAAGATAA
AAAAGATAAATCAACCTCTGTAGAAGAAGAAATCAATACCTTAAAAGTCGAAAAGAATTC
AATTTAAAATTT