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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFC556
Library AF
(Link to library)
Clone ID AFC556
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15327-1
Original site URL
Representative seq. ID AFC556P
(Link to Original site)
Representative DNA sequence 
>AFC556 (AFC556Q) /CSM/AF/AFC5-C/AFC556Q.Seq.d/
ATACATATACATTAAATAATGGCCACATTATCACGTAATTTACGTTTATCATTAGATACA
CCAACCTCAACTTTTGTTAGAACCAAAATTGTTTGTACAATTGGTCCAAAAACAATGAGT
GAAGAAGCTTTAATTAAATTAATCGAAACTGGTATGAACGTTTGTAGACTCAACTTTTCA
CATGGTACTCATGATTATCATGGTCAAGTTATTAAAAATGTTAGATCAGCAATGGAGAAA
ACAGGTAAAATTATTGCTATTATGTTAGATACTAAAGGTCCAGAAATTAGAACTGGTAAA
ATTGAAGATCGTTGTGGTTATGTTGATTTATTCGTTGGCCAAGAAATCCTCGTTGATACC
AATATGAATCAACCAGGTACTTCATTTAGAATTTCAATCGATTATAAAGGTTTATTAGAT
TCAGTTAAAGTTGGTGGTTACATTTTAATTGCTGATGGTGTAATTTCATTATCTATTACT
GCAGTTGAAAAAGAAAAAGGTCATGTTGTTTGTAGAGTTAATAATAATTCACGTCTTGGT
GAAAATAAAAATGTACATTTACCAGGTGCTATTGTTAXXXXXXXXXXTGAAATTTTAGAG
GTTTCCGATGGTATTATGGTTGCTCGTGGTGATTTAGGTGTTGAAGTTCAAATGGAGAAG
ATTTTCGTCGCTCAAAAGATGATCGTTAGTAAATGTAATGCCGCCGGTAAACCAGTAATT
ACCGCCACTCAAATGTTGGAATCAATGATCAAGAATCCAAGACCAACCCGTGCTGAAGCT
ACCGATGTTGCCAATGCCGTTTTAGATGGTTCCGATTGTGTTATGCTTTCAGGTGAAACC
GCAAGCGGTGATTACCCATACGAAGCCGTAGACATTATGGCAAAGATCTGTAGAGAAGCA
GAGTTGGTAGAATCTTCAACTGATTATCAAACTTTATTCGCTGCCCTTAAATTATCATCA
GCCAAACCAGTCAGCATTGCTGAAACCGTTGCTTCTTACGCCGTCGCAACCGCTATCGAT
TTGAAGGCTGATCTCATCATCACACTCACTGAAACCGGTCTCACTGCTCGTCTCGTCAGT
AAATATCGTCCATCCATTCCAATCATTGCCGTCACTTCATGGTCATACACTGTTAAACAT
TTACTCGCCACTAGAGGTGCCATTCCATTCTTGGTAGAATCATTGGTCGGCACTGATAAA
CTCGTTGAATCTTGTCTCGAATATGCTATGAAACATAACTTATGTAAAAAAGGTTCAAGA
GTTGTCATTGTTTCTGGTGTTAGGAAGGTGTACCAGGTAAAACCAACAGTTTAAGAGTTC
TTACCGT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ihih*IMATLSRNLRLSLDTPTSTFVRTKIVCTIGPKTMSEEALIKLIETGMNVCRLNFS
HGTHDYHGQVIKNVRSAMEKTGKIIAIMLDTKGPEIRTGKIEDRCGYVDLFVGQEILVDT
NMNQPGTSFRISIDYKGLLDSVKVGGYILIADGVISLSITAVEKEKGHVVCRVNNNSRLG
ENKNVHLPGAIV---

---EILEVSDGIMVARGDLGVEVQMEKIFVAQKMIVSKCNAAGKPVITATQMLESMIKNP
RPTRAEATDVANAVLDGSDCVMLSGETASGDYPYEAVDIMAKICREAELVESSTDYQTLF
AALKLSSAKPVSIAETVASYAVATAIDLKADLIITLTETGLTARLVSKYRPSIPIIAVTS
WSYTVKHLLATRGAIPFLVESLVGTDKLVESCLEYAMKHNLCKKGSRVVIVSGVRKVYQV
KPTV*eflp
Translated Amino Acid sequence (All Frames) 
Frame A:
ihih*IMATLSRNLRLSLDTPTSTFVRTKIVCTIGPKTMSEEALIKLIETGMNVCRLNFS
HGTHDYHGQVIKNVRSAMEKTGKIIAIMLDTKGPEIRTGKIEDRCGYVDLFVGQEILVDT
NMNQPGTSFRISIDYKGLLDSVKVGGYILIADGVISLSITAVEKEKGHVVCRVNNNSRLG
ENKNVHLPGAIV---

---*nfrgfrwyygcsw*frc*ssngedfrrskddr**m*crr*tsnyrhsnvgindqes
ktnpc*syrccqcrfrwfrlcyafr*nrkr*lpirsrrhygkdl*rsrvgrifn*lsnfi
rcp*iiisqtsqhc*nrcflrrrnryrfeg*shhhth*nrshcssrq*issihsnhcrhf
mvihc*tftrh*rchsilgriigrh**tr*ilsricyet*lm*krfkschcfwc*egvpg
ktnslrvlt

Frame B:
yiyik*wphyhviyvyh*ihqpqlllepklfvqlvqkq*vkkl*ln*sklv*tfvdstfh
mvlmiimvkllkmldqqwrkqvkllllc*ilkvqklelvklkivvvmliyslakksslip
i*inqvlhlefqsiikvy*iqlklvvtf*llmv*fhylllqlkkkkvmlfveliiihvlv
kikmyiyqvlll---

---EILEVSDGIMVARGDLGVEVQMEKIFVAQKMIVSKCNAAGKPVITATQMLESMIKNP
RPTRAEATDVANAVLDGSDCVMLSGETASGDYPYEAVDIMAKICREAELVESSTDYQTLF
AALKLSSAKPVSIAETVASYAVATAIDLKADLIITLTETGLTARLVSKYRPSIPIIAVTS
WSYTVKHLLATRGAIPFLVESLVGTDKLVESCLEYAMKHNLCKKGSRVVIVSGVRKVYQV
KPTV*eflp

Frame C:
tytlnnghiit*ftfiirytnlnfc*nqnclynwsknne*rsfn*inrnwyerl*tqlft
wys*lswssy*kc*isngenr*nycyyvry*rsrn*nw*n*rslwlc*firwprnpr*yq
yestryfi*nfnrl*rfirfs*swwlhfnc*wcnfiiyycs*krkrsccl*s***ftsw*
k*kctftrcyc---

---kf*rfpmvlwllvvi*vlkfkwrrfsslkr*slvnvmppvnq*lpplkcwnq*sriq
dqpvlklpmlpmpf*mvpivlcfqvkpqavithtkp*tlwqrsvekqsw*nlqliiklys
lplnyhqpnqsallkpllltpsqplsi*rlissshslkpvsllvssvnivhpfqslpslh
ghtllniysplevpfhsw*nhwsalinslnlvsnml*nityvkkvqelslflvlgrctr*
nqqfkssyr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFC556 (AFC556Q) /CSM/AF/AFC5-C/AFC556Q.Seq.d/                       2571   0.0
AFD210 (AFD210Q) /CSM/AF/AFD2-A/AFD210Q.Seq.d/                       1431   0.0
VFB872 (VFB872Q) /CSM/VF/VFB8-C/VFB872Q.Seq.d/                       1419   0.0
SFB189 (SFB189Q) /CSM/SF/SFB1-D/SFB189Q.Seq.d/                       1411   0.0
SFF740 (SFF740Q) /CSM/SF/SFF7-B/SFF740Q.Seq.d/                       1360   0.0
AFB422 (AFB422Q) /CSM/AF/AFB4-A/AFB422Q.Seq.d/                       1326   0.0
CFH895 (CFH895Q) /CSM/CF/CFH8-D/CFH895Q.Seq.d/                       1322   0.0
SFG204 (SFG204Q) /CSM/SF/SFG2-A/SFG204Q.Seq.d/                       1316   0.0
CFB142 (CFB142Q) /CSM/CF/CFB1-B/CFB142Q.Seq.d/                       1312   0.0
AFD612 (AFD612Q) /CSM/AF/AFD6-A/AFD612Q.Seq.d/                       1306   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BM159195|BM159195.1 EST561718 PyBS Plasmodium yoelii yoelii cDNA clone PYCJA82 5' end, mRNA sequence.    96  1e-15  1
CB098512|CB098512.1 ku54d11.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to TR:O17835 O17835 PYRUVATE KINASE ;, mRNA sequence.    64  7e-14  3
AW455356|AW455356.1 EST311894 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX10E14 5', mRNA sequence.    76  2e-13  3
AL844505|AL844505.1 Plasmodium falciparum chromosome  6.         107  3e-13  10
BD267398|BD267398.1 Schizochytriumpks gene.                       58  6e-13  6
BD193248|BD193248.1 Production of polyunsaturated fatty acids by expression of polyketide-like synthesis genes in plants.    58  6e-13  6
AR118084|AR118084.1 Sequence 12 from patent US 6140486.           58  6e-13  6
E50268|E50268.1 Gene of docosahexanoic acid-producing bacterium.    58  7e-13  6
AB025342|AB025342.1 Moritella marina genes, complete cds, similar to eicosapentaenoic acid synthesis gene cluster.    58  7e-13  6
BX565550|BX565550.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse78b07_q1c.    66  2e-11  2
dna update 2003. 7.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RF5) RecName: Full=Pyruvate kinase;          Short=PK;      ...   564   e-162
AY128951_1(AY128951|pid:none) Glycine max isolate PyrKinc_1 pyru...   332   2e-89
(Q42806) RecName: Full=Pyruvate kinase, cytosolic isozyme;      ...   331   4e-89
EF678403_1(EF678403|pid:none) Picea sitchensis clone WS0291_A02 ...   330   7e-89
AB050726_1(AB050726|pid:none) Toxoplasma gondii pk mRNA for pyru...   330   7e-89
EF676511_1(EF676511|pid:none) Picea sitchensis clone WS02740_B19...   330   9e-89
DQ083548_1(DQ083548|pid:none) Citrus sinensis pyruvate kinase mR...   324   5e-87
AF242871_1(AF242871|pid:none) Lilium longiflorum cytosolic pyruv...   323   6e-87
BT052934_1(BT052934|pid:none) Medicago truncatula clone MTYFL_FM...   322   1e-86
(Q42954) RecName: Full=Pyruvate kinase, cytosolic isozyme;      ...   322   1e-86
protein update 2009. 3.27
PSORT 

 psg: 0.75  gvh: 0.45  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.46  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: mitochondrial
	 16.0 %: cytoplasmic
	 16.0 %: nuclear
	  4.0 %: plasma membrane

>> prediction for AFC556 is mit
5' end seq. ID AFC556F
5' end seq. 
>AFC556F.Seq
ATACATATACATTAAATAATGGCCACATTATCACGTAATTTACGTTTATCATTAGATACA
CCAACCTCAACTTTTGTTAGAACCAAAATTGTTTGTACAATTGGTCCAAAAACAATGAGT
GAAGAAGCTTTAATTAAATTAATCGAAACTGGTATGAACGTTTGTAGACTCAACTTTTCA
CATGGTACTCATGATTATCATGGTCAAGTTATTAAAAATGTTAGATCAGCAATGGAGAAA
ACAGGTAAAATTATTGCTATTATGTTAGATACTAAAGGTCCAGAAATTAGAACTGGTAAA
ATTGAAGATCGTTGTGGTTATGTTGATTTATTCGTTGGCCAAGAAATCCTCGTTGATACC
AATATGAATCAACCAGGTACTTCATTTAGAATTTCAATCGATTATAAAGGTTTATTAGAT
TCAGTTAAAGTTGGTGGTTACATTTTAATTGCTGATGGTGTAATTTCATTATCTATTACT
GCAGTTGAAAAAGAAAAAGGTCATGTTGTTTGTAGAGTTAATAATAATTCACGTCTTGGT
GAAAATAAAAATGTACATTTACCAGGTGCTATTGTTA----------
Length of 5' end seq. 577
3' end seq. ID AFC556Z
3' end seq. 
>AFC556Z.Seq
----------TGAAATTTTAGAGGTTTCCGATGGTATTATGGTTGCTCGTGGTGATTTAG
GTGTTGAAGTTCAAATGGAGAAGATTTTCGTCGCTCAAAAGATGATCGTTAGTAAATGTA
ATGCCGCCGGTAAACCAGTAATTACCGCCACTCAAATGTTGGAATCAATGATCAAGAATC
CAAGACCAACCCGTGCTGAAGCTACCGATGTTGCCAATGCCGTTTTAGATGGTTCCGATT
GTGTTATGCTTTCAGGTGAAACCGCAAGCGGTGATTACCCATACGAAGCCGTAGACATTA
TGGCAAAGATCTGTAGAGAAGCAGAGTTGGTAGAATCTTCAACTGATTATCAAACTTTAT
TCGCTGCCCTTAAATTATCATCAGCCAAACCAGTCAGCATTGCTGAAACCGTTGCTTCTT
ACGCCGTCGCAACCGCTATCGATTTGAAGGCTGATCTCATCATCACACTCACTGAAACCG
GTCTCACTGCTCGTCTCGTCAGTAAATATCGTCCATCCATTCCAATCATTGCCGTCACTT
CATGGTCATACACTGTTAAACATTTACTCGCCACTAGAGGTGCCATTCCATTCTTGGTAG
AATCATTGGTCGGCACTGATAAACTCGTTGAATCTTGTCTCGAATATGCTATGAAACATA
ACTTATGTAAAAAAGGTTCAAGAGTTGTCATTGTTTCTGGTGTTAGGAAGGTGTACCAGG
TAAAACCAACAGTTTAAGAGTTCTTACCGT
Length of 3' end seq. 740
Connected seq. ID AFC556P
Connected seq. 
>AFC556P.Seq
ATACATATACATTAAATAATGGCCACATTATCACGTAATTTACGTTTATCATTAGATACA
CCAACCTCAACTTTTGTTAGAACCAAAATTGTTTGTACAATTGGTCCAAAAACAATGAGT
GAAGAAGCTTTAATTAAATTAATCGAAACTGGTATGAACGTTTGTAGACTCAACTTTTCA
CATGGTACTCATGATTATCATGGTCAAGTTATTAAAAATGTTAGATCAGCAATGGAGAAA
ACAGGTAAAATTATTGCTATTATGTTAGATACTAAAGGTCCAGAAATTAGAACTGGTAAA
ATTGAAGATCGTTGTGGTTATGTTGATTTATTCGTTGGCCAAGAAATCCTCGTTGATACC
AATATGAATCAACCAGGTACTTCATTTAGAATTTCAATCGATTATAAAGGTTTATTAGAT
TCAGTTAAAGTTGGTGGTTACATTTTAATTGCTGATGGTGTAATTTCATTATCTATTACT
GCAGTTGAAAAAGAAAAAGGTCATGTTGTTTGTAGAGTTAATAATAATTCACGTCTTGGT
GAAAATAAAAATGTACATTTACCAGGTGCTATTGTTA----------TGAAATTTTAGAG
GTTTCCGATGGTATTATGGTTGCTCGTGGTGATTTAGGTGTTGAAGTTCAAATGGAGAAG
ATTTTCGTCGCTCAAAAGATGATCGTTAGTAAATGTAATGCCGCCGGTAAACCAGTAATT
ACCGCCACTCAAATGTTGGAATCAATGATCAAGAATCCAAGACCAACCCGTGCTGAAGCT
ACCGATGTTGCCAATGCCGTTTTAGATGGTTCCGATTGTGTTATGCTTTCAGGTGAAACC
GCAAGCGGTGATTACCCATACGAAGCCGTAGACATTATGGCAAAGATCTGTAGAGAAGCA
GAGTTGGTAGAATCTTCAACTGATTATCAAACTTTATTCGCTGCCCTTAAATTATCATCA
GCCAAACCAGTCAGCATTGCTGAAACCGTTGCTTCTTACGCCGTCGCAACCGCTATCGAT
TTGAAGGCTGATCTCATCATCACACTCACTGAAACCGGTCTCACTGCTCGTCTCGTCAGT
AAATATCGTCCATCCATTCCAATCATTGCCGTCACTTCATGGTCATACACTGTTAAACAT
TTACTCGCCACTAGAGGTGCCATTCCATTCTTGGTAGAATCATTGGTCGGCACTGATAAA
CTCGTTGAATCTTGTCTCGAATATGCTATGAAACATAACTTATGTAAAAAAGGTTCAAGA
GTTGTCATTGTTTCTGGTGTTAGGAAGGTGTACCAGGTAAAACCAACAGTTTAAGAGTTC
TTACCGT
Length of connected seq. 1317
Full length Seq ID -
Full length Seq. -
Length of full length seq. -