>AHH473 (AHH473Q) /CSM/AH/AHH4-D/AHH473Q.Seq.d/
GAAAAATTATTTCGTTAATATGTATAGAAATATATATATATAAAATAAATTTGTAAATAT
TAAAAATCAAGGTAAATGGATAAATTAGAAAGGATAGAGTTAGAATCACAAGTATGTAAT
GAATTAGAAAGGTTTATTGGAAGTGGTGACAAACTTTTAGCCGAGTTTGTTATTGGTTTA
GCTGACGAAAATCCTAAACTAAAGGATTTCAATAAAGCAATATCAGAGAATGTACCAGAT
TTTCCAGAATCATTAAGTTCACATTTATTTAATCTAATTGAAAAAATGAAAAAGAAAACA
ACAACAACAACTAATAATAATAATAATAATAATAATAATAATACCAACACAGCAAAAACA
ACAACTACAACAACAACAACAACAACTACAACTAATAATAATAATTATAAAGAGTCAGAA
TGGGAAGAAACCAAATTAAATTCAAATTCTAATAATCAAAAGAAAAATCAATTTCCAGGA
TTATCAATACCAXXXXXXXXXXATTGTCAAAGATGTTAATAGCCTCAGTAGATCTCGGTT
GTAGTGATGAGATTCTAACCGTGGTGGCAATGTTATCAGTTCAAAATGTTTTCTATCGTC
CAAAAGAGAAACAAGCTTTAGCCGATCAAAAGAAAGCAAAATTCTTTCAACCTGAAGGTG
ATCATCTAACTCTACTAAACGTTTATGAATCTTGGAAAAATAGTAAATTCAGTAATCCTT
GGTGTTTTGAAAACTTTGTTCAGGCTCGTTCCCTTCGTAGAGCTCAAGACGTTAGAAAAC
AATTGATCACAATTATGGATCGTTACAAATTGGATATCATTTCCGCCGGTAGAAACTATA
CAAAAATTCAAAAGGCAATCTGTTCGGGTTTCTTTGCAAACGCTAGCAAAAAAGATCCAA
ATGAAGGCTATAAAACTTTGGTCGAGGGTCAACCAGTTTACATTCATCCTTCCTCCACTC
TCTTCAATAGAAATCCCGATTGGGTAATCTATCATGAGTTGGTTATGACCACCAAAGAAT
ATATGAGAGAGGTTTGTACCATCGATCCAAAATGGTTGGTTGAATTGGCTCCAAAATTCT
TTAAAACTTCTGATCCAAATAAAATTTCAAAAAGAAAAAGAAAAGAAAAAATTGAACCAC
TTTATGATAAATATAATGATCCAAATGCTTGGCGTCCTTCAAAAAGAAAATAATTAACTA
ATTAAACTAAAAAAAAAAA

eklfr*yv*kyiyik*icky*ksr*MDKLERIELESQVCNELERFIGSGDKLLAEFVIGL
ADENPKLKDFNKAISENVPDFPESLSSHLFNLIEKMKKKTTTTTNNNNNNNNNNTNTAKT
TTTTTTTTTTTNNNNYKESEWEETKLNSNSNNQKKNQFPGLSIP---

---LSKMLIASVDLGCSDEILTVVAMLSVQNVFYRPKEKQALADQKKAKFFQPEGDHLTL
LNVYESWKNSKFSNPWCFENFVQARSLRRAQDVRKQLITIMDRYKLDIISAGRNYTKIQK
AICSGFFANASKKDPNEGYKTLVEGQPVYIHPSSTLFNRNPDWVIYHELVMTTKEYMREV
CTIDPKWLVELAPKFFKTSDPNKISKRKRKEKIEPLYDKYNDPNAWRPSKRK*ltn*tkk
k

Frame A:
eklfr*yv*kyiyik*icky*ksr*MDKLERIELESQVCNELERFIGSGDKLLAEFVIGL
ADENPKLKDFNKAISENVPDFPESLSSHLFNLIEKMKKKTTTTTNNNNNNNNNNTNTAKT
TTTTTTTTTTTNNNNYKESEWEETKLNSNSNNQKKNQFPGLSIP---

---ivkdvnslsrsrl***dsnrggnvisskcflsskretsfsrskeskilst*r*ssns
tkrl*ilek**iq*slvf*klcsgsfps*ssrr*ktidhnygslqigyhfrr*klyknsk
gnlfgflckr*qkrsk*rl*nfgrgstslhssflhslq*ksrlgnls*vgydhqriyerg
lyhrskmvg*igskil*nf*sk*nfkkkkkrkn*ttl**i**skclasfkkkiin*ln*k
kk

Frame B:
knyfvnmyrniyi*nkfvniknqgkwin*kg*s*nhkyvmn*kgllevvtnf*pslllv*
ltkiln*risikqyqrmyqifqnh*vhiyli*lkk*krkqqqqliiiiiiiiiiptqqkq
qlqqqqqqlqliiiiiksqngkkpn*iqiliikrkinfqdyqy---

---LSKMLIASVDLGCSDEILTVVAMLSVQNVFYRPKEKQALADQKKAKFFQPEGDHLTL
LNVYESWKNSKFSNPWCFENFVQARSLRRAQDVRKQLITIMDRYKLDIISAGRNYTKIQK
AICSGFFANASKKDPNEGYKTLVEGQPVYIHPSSTLFNRNPDWVIYHELVMTTKEYMREV
CTIDPKWLVELAPKFFKTSDPNKISKRKRKEKIEPLYDKYNDPNAWRPSKRK*ltn*tkk
k

Frame C:
kiislicieiyiykinl*ilkikvng*irkdrvritsm**irkvywkw*qtfsrvcywfs
*rks*tkgfq*snirectrfsriikftfi*sn*knekennnnn**********yqhsknn
nynnnnnnyn****l*rvrmgrnqikfkf**skeksisriint---

---cqrc**pq*isvvvmrf*pwwqcyqfkmfsivqkrnkl*pikrkqnsfnlkvii*ly
*tfmnlgkivnsvilgvlktlfrlvpfvelktlenn*sqlwivtnwisfppvetiqkfkr
qsvrvslqtlakkiqmkaiklwsrvnqftfilpplssieipig*simswl*ppkni*erf
vpsiqngwlnwlqnslklliqikfqkekekkklnhfminimiqmlgvlqkenn*liklkk
k

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AHH473 (AHH473Q) /CSM/AH/AHH4-D/AHH473Q.Seq.d/                       1360   0.0
SHE426 (SHE426Q) /CSM/SH/SHE4-B/SHE426Q.Seq.d/                       1187   0.0
SSA122 (SSA122Q) /CSM/SS/SSA1-A/SSA122Q.Seq.d/                         42   0.020
SSI251 (SSI251Q) /CSM/SS/SSI2-C/SSI251Q.Seq.d/                         40   0.078
SSH860 (SSH860Q) /CSM/SS/SSH8-C/SSH860Q.Seq.d/                         40   0.078
SLI660 (SLI660Q) /CSM/SL/SLI6-C/SLI660Q.Seq.d/                         40   0.078
SLH695 (SLH695Q) /CSM/SL/SLH6-D/SLH695Q.Seq.d/                         40   0.078
VSK259 (VSK259Q) /CSM/VS/VSK2-C/VSK259Q.Seq.d/                         38   0.31
VSI703 (VSI703Q) /CSM/VS/VSI7-A/VSI703Q.Seq.d/                         38   0.31
VSI660 (VSI660Q) /CSM/VS/VSI6-C/VSI660Q.Seq.d/                         38   0.31

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU011297|BU011297.1 QGJ15N08.yg.ab1 QG_EFGHJ lettuce serriola Lactuca sativa cDNA cloneQGJ15N08, mRNA sequence.    66   4e-15  3
CD096879|CD096879.1 ME1-0010T-M140-D04-U.G ME1-0010 Schistosoma mansoni cDNA clone ME1-0010T-M140-D04.G, mRNA sequence.    56   2e-12  2
BM883880|BM883880.1 rc23h06.y1 Meloidogyne hapla egg pAMP1 v1 Meloidogyne hapla cDNA 5' similar to SW:DDX8_CAEEL Q09530 PUTATIVE PRE-MRNA SPLICING FACTOR ATP-DEPENDENT RNA HELICASE EEED8.5. [1] ;, mRNA sequence.    68   3e-08  2
AW587914|AW587914.1 kp09f11.y1 TBN95TM-SSFH Strongyloides stercoralis cDNA 5' similar to WP:EEED8.5 CE01889 RNA HELICASE ;, mRNA sequence.    50   2e-07  3
CA980915|CA980915.1 AGENCOURT_11283504 Wellcome CRC pSK egg Xenopus laevis cDNA clone IMAGE:6869593 5', mRNA sequence.    48   2e-06  2
BE471386|BE471386.1 EST416239 potato stolon, Cornell University Solanum tuberosum cDNA clone cSTA28P4, mRNA sequence.    46   1e-05  3
CB823869|CB823869.1 EST 5093 Ripe Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone bh002b18 3', mRNA sequence.    46   1e-04  2
AL929356|AL929356.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 2/5.    34   2e-04  13
BW286590|BW286590.1 Ciona intestinalis cDNA, clone:cigd030i09, 5' end, single read.    58   3e-04  1
AI732008|AI732008.1 BNLGHi13826 Six-day Cotton fiber Gossypium hirsutum cDNA 5' similar to PUTATIVE PRE-MRNA SPLICING FACTOR, mRNA sequence.    48   8e-04  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54F05) RecName: Full=ATP-dependent RNA helicase dhx8;         ...   440   e-122
AP008208_1045(AP008208|pid:none) Oryza sativa (japonica cultivar...   360   7e-98
CP000584_215(CP000584|pid:none) Ostreococcus lucimarinus CCE9901...   358   3e-97
AK222021_1(AK222021|pid:none) Arabidopsis thaliana mRNA for ATP-...   354   3e-96
X97970_1(X97970|pid:none) A.thaliana mRNA for RNA helicase.  &X9...   354   3e-96
(A2A4P0) RecName: Full=ATP-dependent RNA helicase DHX8;         ...   350   7e-95
BC022656_1(BC022656|pid:none) Mus musculus DEAH (Asp-Glu-Ala-His...   350   7e-95
(Q14562) RecName: Full=ATP-dependent RNA helicase DHX8;         ...   350   7e-95
AK142226_1(AK142226|pid:none) Mus musculus 13 days embryo heart ...   350   7e-95
CR861244_1(CR861244|pid:none) Pongo abelii mRNA; cDNA DKFZp459H0...   350   7e-95

 psg: 0.75  gvh: 0.34  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.08  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AHH473 is cyt

>AHH473F.Seq
GAAAAATTATTTCGTTAATATGTATAGAAATATATATATATAAAATAAATTTGTAAATAT
TAAAAATCAAGGTAAATGGATAAATTAGAAAGGATAGAGTTAGAATCACAAGTATGTAAT
GAATTAGAAAGGTTTATTGGAAGTGGTGACAAACTTTTAGCCGAGTTTGTTATTGGTTTA
GCTGACGAAAATCCTAAACTAAAGGATTTCAATAAAGCAATATCAGAGAATGTACCAGAT
TTTCCAGAATCATTAAGTTCACATTTATTTAATCTAATTGAAAAAATGAAAAAGAAAACA
ACAACAACAACTAATAATAATAATAATAATAATAATAATAATACCAACACAGCAAAAACA
ACAACTACAACAACAACAACAACAACTACAACTAATAATAATAATTATAAAGAGTCAGAA
TGGGAAGAAACCAAATTAAATTCAAATTCTAATAATCAAAAGAAAAATCAATTTCCAGGA
TTATCAATACCANNNNNNNNNN

>AHH473Z.Seq
NNNNNNNNNNATTGTCAAAGATGTTAATAGCCTCAGTAGATCTCGGTTGTAGTGATGAGA
TTCTAACCGTGGTGGCAATGTTATCAGTTCAAAATGTTTTCTATCGTCCAAAAGAGAAAC
AAGCTTTAGCCGATCAAAAGAAAGCAAAATTCTTTCAACCTGAAGGTGATCATCTAACTC
TACTAAACGTTTATGAATCTTGGAAAAATAGTAAATTCAGTAATCCTTGGTGTTTTGAAA
ACTTTGTTCAGGCTCGTTCCCTTCGTAGAGCTCAAGACGTTAGAAAACAATTGATCACAA
TTATGGATCGTTACAAATTGGATATCATTTCCGCCGGTAGAAACTATACAAAAATTCAAA
AGGCAATCTGTTCGGGTTTCTTTGCAAACGCTAGCAAAAAAGATCCAAATGAAGGCTATA
AAACTTTGGTCGAGGGTCAACCAGTTTACATTCATCCTTCCTCCACTCTCTTCAATAGAA
ATCCCGATTGGGTAATCTATCATGAGTTGGTTATGACCACCAAAGAATATATGAGAGAGG
TTTGTACCATCGATCCAAAATGGTTGGTTGAATTGGCTCCAAAATTCTTTAAAACTTCTG
ATCCAAATAAAATTTCAAAAAGAAAAAGAAAAGAAAAAATTGAACCACTTTATGATAAAT
ATAATGATCCAAATGCTTGGCGTCCTTCAAAAAGAAAATAATTAACTAATTAAACTAAAA
AAAAAAA

>AHH473P.Seq
GAAAAATTATTTCGTTAATATGTATAGAAATATATATATATAAAATAAATTTGTAAATAT
TAAAAATCAAGGTAAATGGATAAATTAGAAAGGATAGAGTTAGAATCACAAGTATGTAAT
GAATTAGAAAGGTTTATTGGAAGTGGTGACAAACTTTTAGCCGAGTTTGTTATTGGTTTA
GCTGACGAAAATCCTAAACTAAAGGATTTCAATAAAGCAATATCAGAGAATGTACCAGAT
TTTCCAGAATCATTAAGTTCACATTTATTTAATCTAATTGAAAAAATGAAAAAGAAAACA
ACAACAACAACTAATAATAATAATAATAATAATAATAATAATACCAACACAGCAAAAACA
ACAACTACAACAACAACAACAACAACTACAACTAATAATAATAATTATAAAGAGTCAGAA
TGGGAAGAAACCAAATTAAATTCAAATTCTAATAATCAAAAGAAAAATCAATTTCCAGGA
TTATCAATACCA----------ATTGTCAAAGATGTTAATAGCCTCAGTAGATCTCGGTT
GTAGTGATGAGATTCTAACCGTGGTGGCAATGTTATCAGTTCAAAATGTTTTCTATCGTC
CAAAAGAGAAACAAGCTTTAGCCGATCAAAAGAAAGCAAAATTCTTTCAACCTGAAGGTG
ATCATCTAACTCTACTAAACGTTTATGAATCTTGGAAAAATAGTAAATTCAGTAATCCTT
GGTGTTTTGAAAACTTTGTTCAGGCTCGTTCCCTTCGTAGAGCTCAAGACGTTAGAAAAC
AATTGATCACAATTATGGATCGTTACAAATTGGATATCATTTCCGCCGGTAGAAACTATA
CAAAAATTCAAAAGGCAATCTGTTCGGGTTTCTTTGCAAACGCTAGCAAAAAAGATCCAA
ATGAAGGCTATAAAACTTTGGTCGAGGGTCAACCAGTTTACATTCATCCTTCCTCCACTC
TCTTCAATAGAAATCCCGATTGGGTAATCTATCATGAGTTGGTTATGACCACCAAAGAAT
ATATGAGAGAGGTTTGTACCATCGATCCAAAATGGTTGGTTGAATTGGCTCCAAAATTCT
TTAAAACTTCTGATCCAAATAAAATTTCAAAAAGAAAAAGAAAAGAAAAAATTGAACCAC
TTTATGATAAATATAATGATCCAAATGCTTGGCGTCCTTCAAAAAGAAAATAATTAACTA
ATTAAACTAAAAAAAAAAA