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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFA173
Library CF
(Link to library)
Clone ID CFA173
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12870-1
Original site URL
Representative seq. ID CFA173P
(Link to Original site)
Representative DNA sequence 
>CFA173 (CFA173Q) /CSM/CF/CFA1-D/CFA173Q.Seq.d/
ATCGCCATTGATTTCGCTCGTAAGGAAATGGTTATTTTGGGTACTCAATACGCTGGTGAA
ATGAAAAAAAGGTATTCTCACCATCATGATGTATCTCATGCCAAAGATGGGTGTCTTACC
ATTACATTCCTCTTGCAATCAAGCTCGTAACAATGGTGATACCACATTATTCTTTGGTCT
CTCTGGTACTGGTAAAACTACATTATCCGCCGATATTAACCGTGAATTAATTGGTGATGA
TGAACATGTTTGGACCGATACTGGTTGTTTCAATATCGAAGGTGGTTGCTATGCAAAATG
TATCGATCTCTCTCGTGAAAAGGAACCAGAGATCTTTGATGCCATCAAGTTTGGTGCTGT
TTTAGAGAATGTCGTCTACAATGAGTACTCTCGTAAGGTCGATTACAATGATGTCTCTAT
CACTGAGAAXXXXXXXXXXGNATTAAANTGGCCTACNCTNGNGCCATCATCGATGCCATT
NNCAGTGGANAACTTNAAAAAATTCCNACCCCTAAAATGGATGTTTTCGGTTTCCAAGNA
CCAAATAGTTGNCCAGGTGTTCCATNTGAAATTTTAATGCCNATTAANGGNTGGGCTGAT
AAAAANAAATATGTTTCAACTATGCATAAATTGGNTNAATTATTTATTGAAAATTTCNAA
AAATTCCAANATAAAGCTTCNCCANAACNTG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
splislvrkwlfwvlntlvk*KKGILTIMMYLMPKMGVLPLHSSCNQARNNGDTTLFFGL
SGTGKTTLSADINRELIGDDEHVWTDTGCFNIEGGCYAKCIDLSREKEPEIFDAIKFGAV
LENVVYNEYSRKVDYNDVSITE---

---IKXAYXXAIIDAIXSGXLXKIPTPKMDVFGFQXPNSXPGVPXEILMPIXGWADKXKY
VSTMHKLXXLFIENFXKFQXKASPXX
Translated Amino Acid sequence (All Frames) 
Frame A:
iaidfarkemvilgtqyagemkkryshhhdvshakdgcltitfllqsss*qw*yhiilws
lwyw*nyiirry*p*inw***tcldrywlfqyrrwllckmyrsls*kgtrdl*chqvwcc
frecrlq*vls*grlq*clyh*e---

---xlxwptlxpssmpxxvxnxkkfxplkwmfsvskxqivxqvfhxkf*cxlxxglikxn
mfqlcinwxnyllkisknsxiklxxnx

Frame B:
splislvrkwlfwvlntlvk*KKGILTIMMYLMPKMGVLPLHSSCNQARNNGDTTLFFGL
SGTGKTTLSADINRELIGDDEHVWTDTGCFNIEGGCYAKCIDLSREKEPEIFDAIKFGAV
LENVVYNEYSRKVDYNDVSITE---

---x*xglxxxhhrchxqwxtxknsxp*ngcfrfpxtk*lxrcsx*nfnax*xxg**kxi
cfnya*igxiiy*kfxkipx*sfxxt

Frame C:
rh*frs*gngyfgysirw*nekkvfsps*ciscqrwvsyhyiplaiklvtmviphyslvs
lvlvklhyppiltvn*lvmmnmfgpilvvsiskvvamqnvsislvkrnqrslmpsslvlf
*rmsstmstlvrsitmmslslr---

---IKXAYXXAIIDAIXSGXLXKIPTPKMDVFGFQXPNSXPGVPXEILMPIXGWADKXKY
VSTMHKLXXLFIENFXKFQXKASPXX
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFA173 (CFA173Q) /CSM/CF/CFA1-D/CFA173Q.Seq.d/                       1090   0.0
CFG166 (CFG166Q) /CSM/CF/CFG1-C/CFG166Q.Seq.d/                        799   0.0
CFA501 (CFA501Q) /CSM/CF/CFA5-A/CFA501Q.Seq.d/                        799   0.0
AFL325 (AFL325Q) /CSM/AF/AFL3-B/AFL325Q.Seq.d/                        724   0.0
AFO776 (AFO776Q) /CSM/AF/AFO7-D/AFO776Q.Seq.d/                        654   0.0
CFE293 (CFE293Q) /CSM/CF/CFE2-D/CFE293Q.Seq.d/                        488   e-137
SLC233 (SLC233Q) /CSM/SL/SLC2-B/SLC233Q.Seq.d/                        408   e-113
AFL109 (AFL109Q) /CSM/AF/AFL1-A/AFL109Q.Seq.d/                        315   5e-85
SLG272 (SLG272Q) /CSM/SL/SLG2-C/SLG272Q.Seq.d/                        307   1e-82
SLA884 (SLA884Q) /CSM/SL/SLA8-D/SLA884Q.Seq.d/                        307   1e-82
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116920|AC116920.2 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.   712  0.0  4
AL423880|AL423880.1 T7 end of clone AZ0AA011A11 of library AZ0AA from strain CBS 712 of Kluyveromyces marxianus.    56  2e-23  5
AX536984|AX536984.1 Sequence 585 from Patent WO02064766.          56  3e-19  4
AX489612|AX489612.1 Sequence 6912 from Patent WO02053728.         56  3e-19  4
U70473|U70473.1 Candida albicans PEP carboxykinase (PCK1) gene, complete cds.    56  3e-18  4
AX617820|AX617820.1 Sequence 783 from Patent WO02094868.          54  4e-18  4
U51133|U51133.1 Staphylococcus aureus phosphoenolpyruvate carboxykinase (pcka) gene, complete cds.    54  6e-18  4
L42943|L42943.1 Staphylococcus aureus (clone KIN50) phosphoenolpyruvate carboxykinase (pckA) gene, complete cds.    54  9e-18  4
AX537040|AX537040.1 Sequence 641 from Patent WO02064766.          56  7e-16  4
AR354025|AR354025.1 Sequence 143 from patent US 6593114.          54  8e-16  4
dna update 2003. 9.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q6FRR0) RecName: Full=Phosphoenolpyruvate carboxykinase [ATP]; ...   187   6e-51
FM211855_1(FM211855|pid:none) Melinis repens partial pckE gene f...   181   2e-50
FM211861_1(FM211861|pid:none) Setaria plicata partial pck gene f...   181   2e-50
AB075548_1(AB075548|pid:none) Toxoplasma gondii pck mRNA for pho...   186   3e-50
FM992695_19(FM992695|pid:none) Candida dubliniensis CD36 chromos...   184   9e-50
FM211878_1(FM211878|pid:none) Tristachya leucothrix partial pck ...   180   9e-50
(Q9T074) RecName: Full=Phosphoenolpyruvate carboxykinase [ATP]; ...   181   1e-49
AF532733_1(AF532733|pid:none) Panicum maximum phosphoenolpyruvat...   180   1e-49
X13096_1(X13096|pid:none) Yeast gene for phosphoenolpyruvate car...   184   1e-49
FM211885_1(FM211885|pid:none) Digitaria sanguinalis partial mRNA...   180   1e-49
protein update 2009. 5.11
PSORT 

 psg: 0.96  gvh: 0.47  alm: 0.36  top: 0.50  tms: 0.00  mit: 0.32  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for CFA173 is cyt
5' end seq. ID CFA173F
5' end seq. 
>CFA173F.Seq
ATCGCCATTGATTTCGCTCGTAAGGAAATGGTTATTTTGGGTACTCAATACGCTGGTGAA
ATGAAAAAAAGGTATTCTCACCATCATGATGTATCTCATGCCAAAGATGGGTGTCTTACC
ATTACATTCCTCTTGCAATCAAGCTCGTAACAATGGTGATACCACATTATTCTTTGGTCT
CTCTGGTACTGGTAAAACTACATTATCCGCCGATATTAACCGTGAATTAATTGGTGATGA
TGAACATGTTTGGACCGATACTGGTTGTTTCAATATCGAAGGTGGTTGCTATGCAAAATG
TATCGATCTCTCTCGTGAAAAGGAACCAGAGATCTTTGATGCCATCAAGTTTGGTGCTGT
TTTAGAGAATGTCGTCTACAATGAGTACTCTCGTAAGGTCGATTACAATGATGTCTCTAT
CACTGAGAA----------
Length of 5' end seq. 429
3' end seq. ID CFA173Z
3' end seq. 
>CFA173Z.Seq
----------GNATTAAANTGGCCTACNCTNGNGCCATCATCGATGCCATTNNCAGTGGA
NAACTTNAAAAAATTCCNACCCCTAAAATGGATGTTTTCGGTTTCCAAGNACCAAATAGT
TGNCCAGGTGTTCCATNTGAAATTTTAATGCCNATTAANGGNTGGGCTGATAAAAANAAA
TATGTTTCAACTATGCATAAATTGGNTNAATTATTTATTGAAAATTTCNAAAAATTCCAA
NATAAAGCTTCNCCANAACNTG
Length of 3' end seq. 252
Connected seq. ID CFA173P
Connected seq. 
>CFA173P.Seq
ATCGCCATTGATTTCGCTCGTAAGGAAATGGTTATTTTGGGTACTCAATACGCTGGTGAA
ATGAAAAAAAGGTATTCTCACCATCATGATGTATCTCATGCCAAAGATGGGTGTCTTACC
ATTACATTCCTCTTGCAATCAAGCTCGTAACAATGGTGATACCACATTATTCTTTGGTCT
CTCTGGTACTGGTAAAACTACATTATCCGCCGATATTAACCGTGAATTAATTGGTGATGA
TGAACATGTTTGGACCGATACTGGTTGTTTCAATATCGAAGGTGGTTGCTATGCAAAATG
TATCGATCTCTCTCGTGAAAAGGAACCAGAGATCTTTGATGCCATCAAGTTTGGTGCTGT
TTTAGAGAATGTCGTCTACAATGAGTACTCTCGTAAGGTCGATTACAATGATGTCTCTAT
CACTGAGAA----------GNATTAAANTGGCCTACNCTNGNGCCATCATCGATGCCATT
NNCAGTGGANAACTTNAAAAAATTCCNACCCCTAAAATGGATGTTTTCGGTTTCCAAGNA
CCAAATAGTTGNCCAGGTGTTCCATNTGAAATTTTAATGCCNATTAANGGNTGGGCTGAT
AAAAANAAATATGTTTCAACTATGCATAAATTGGNTNAATTATTTATTGAAAATTTCNAA
AAATTCCAANATAAAGCTTCNCCANAACNTG
Length of connected seq. 681
Full length Seq ID -
Full length Seq. -
Length of full length seq. -