>CFF414 (CFF414Q) /CSM/CF/CFF4-A/CFF414Q.Seq.d/
AAATACATATACATATACATTAAATAATGGCCACATTATCACGTAATTTACGTTTATCAT
TAGATACACCAACCTCAACTTTTGTTAGAACCAAAATTGTTTGTACAATTGGTCCAAAAA
CAATGAGTGAAGAAGCTTTAATTAAATTAATCGAAACTGGTATGAACGTTTGTAGACTCA
ACTTTTCACATGGTACTCATGATTATCATGGTCAAGTTATTAAAAATGTTAGATCAGCAA
TGGAGAAAACAGGTAAAATTATTGCTATTATGTTAGATACTAAAGGTCCAGAAATTAGAA
CTGGTAAAATTGAAGATCGTTGTGGTTATGTTGATTTATTCGTTGGCCAAGAAATCCTCG
TTGATACCAATATGAATCAACCAGGTACTTCATTTAGAATTTCAATCGATTATAAAGGTT
TATTAGATTCAGTTAAAGTTGGTGGTTACATTTTAATTGCTGATGGTGTAATTTCATTAT
CTATTACTGCAGTTGAAAAAGAAAAAGGTCATGTTGTTTGTAGAGTTXXXXXXXXXXTTC
GTCGCTCAAAAGATGATCGTTAGTAAATGTAATGCCGCCGGTAAACCAGTAATTACCGCC
ACTCAAATGTTGGAATCAATGATCAAGAATCCAAGACCAACCCGTGCTGAAGCTACCGAT
GTTGCCAATGCCGTTTTAGATGGTTCCGATTGTGTTATGCTTTCAGGTGAAACCGCAAGC
GGTGATTACCCATACGAAGCCGTAGACATTATGGCAAAGATCTGTAGAGAAGCAGAGTTG
GTAGAATCTTCAACTGATTATCAAACTTTATTCGCTGCCCTTAAATTATCATCAGCCAAA
CCAGTCAGCATTGCTGAAACCGTTGCTTCTTACGCCGTCGCAACCGCTATCGATTTGAAG
GCTGATCTCATCATCACACTCACTGAAACCGGTCTCACTGCTCGTCTCGTCAGTAAATAT
CGTCCATCCATTCCAATCATTGCCGTCACTTCATGGTCATACACTGTCAAACATTTACTC
GCCACTAGAGGTGCCATTCCATTCTTGGTAGAATCATTGGTCGGCACTGATAAACTCGTT
GAATCTTGTCTCGAATATGCTATGAAACATAACTTATGTAAAAAAGGTTCAAGAGTTGTC
ATTGTTTCTGGTGTTATGGAAGGTGTACCAGGTAAAACCAACAGTTTAAGAGTTCTTACC
GTTGGTGAATCAATCAAAGATTTAAAGAAAAAATAAAAATATAGCCATAATTTTAATAAG
CGCAGA

ihihih*IMATLSRNLRLSLDTPTSTFVRTKIVCTIGPKTMSEEALIKLIETGMNVCRLN
FSHGTHDYHGQVIKNVRSAMEKTGKIIAIMLDTKGPEIRTGKIEDRCGYVDLFVGQEILV
DTNMNQPGTSFRISIDYKGLLDSVKVGGYILIADGVISLSITAVEKEKGHVVCRV---

---FVAQKMIVSKCNAAGKPVITATQMLESMIKNPRPTRAEATDVANAVLDGSDCVMLSG
ETASGDYPYEAVDIMAKICREAELVESSTDYQTLFAALKLSSAKPVSIAETVASYAVATA
IDLKADLIITLTETGLTARLVSKYRPSIPIIAVTSWSYTVKHLLATRGAIPFLVESLVGT
DKLVESCLEYAMKHNLCKKGSRVVIVSGVMEGVPGKTNSLRVLTVGESIKDLKKK*kysh
nfnkrr

Frame A:
kyiyiyik*wphyhviyvyh*ihqpqlllepklfvqlvqkq*vkkl*ln*sklv*tfvds
tfhmvlmiimvkllkmldqqwrkqvkllllc*ilkvqklelvklkivvvmliyslakkss
lipi*inqvlhlefqsiikvy*iqlklvvtf*llmv*fhylllqlkkkkvmlfve---

---FVAQKMIVSKCNAAGKPVITATQMLESMIKNPRPTRAEATDVANAVLDGSDCVMLSG
ETASGDYPYEAVDIMAKICREAELVESSTDYQTLFAALKLSSAKPVSIAETVASYAVATA
IDLKADLIITLTETGLTARLVSKYRPSIPIIAVTSWSYTVKHLLATRGAIPFLVESLVGT
DKLVESCLEYAMKHNLCKKGSRVVIVSGVMEGVPGKTNSLRVLTVGESIKDLKKK*kysh
nfnkrr

Frame B:
ntytytlnnghiit*ftfiirytnlnfc*nqnclynwsknne*rsfn*inrnwyerl*tq
lftwys*lswssy*kc*isngenr*nycyyvry*rsrn*nw*n*rslwlc*firwprnpr
*yqyestryfi*nfnrl*rfirfs*swwlhfnc*wcnfiiyycs*krkrsccl*s---

---sslkr*slvnvmppvnq*lpplkcwnq*sriqdqpvlklpmlpmpf*mvpivlcfqv
kpqavithtkp*tlwqrsvekqsw*nlqliiklyslplnyhqpnqsallkpllltpsqpl
si*rlissshslkpvsllvssvnivhpfqslpslhghtlsniysplevpfhsw*nhwsal
inslnlvsnml*nityvkkvqelslflvlwkvyqvkptv*eflplvnqski*rknkniai
ilisa

Frame C:
ihihih*IMATLSRNLRLSLDTPTSTFVRTKIVCTIGPKTMSEEALIKLIETGMNVCRLN
FSHGTHDYHGQVIKNVRSAMEKTGKIIAIMLDTKGPEIRTGKIEDRCGYVDLFVGQEILV
DTNMNQPGTSFRISIDYKGLLDSVKVGGYILIADGVISLSITAVEKEKGHVVCRV---

---rrskddr**m*crr*tsnyrhsnvgindqesktnpc*syrccqcrfrwfrlcyafr*
nrkr*lpirsrrhygkdl*rsrvgrifn*lsnfircp*iiisqtsqhc*nrcflrrrnry
rfeg*shhhth*nrshcssrq*issihsnhcrhfmvihcqtftrh*rchsilgriigrh*
*tr*ilsricyet*lm*krfkschcfwcygrctr*nqqfkssyrw*inqrfkekiki*p*
f**aq

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFF414 (CFF414Q) /CSM/CF/CFF4-A/CFF414Q.Seq.d/                       2379   0.0
CFC605 (CFC605Q) /CSM/CF/CFC6-A/CFC605Q.Seq.d/                       1366   0.0
AFF443 (AFF443Q) /CSM/AF/AFF4-B/AFF443Q.Seq.d/                       1366   0.0
AFE508 (AFE508Q) /CSM/AF/AFE5-A/AFE508Q.Seq.d/                       1366   0.0
SFI138 (SFI138Q) /CSM/SF/SFI1-B/SFI138Q.Seq.d/                       1364   0.0
SFC160 (SFC160Q) /CSM/SF/SFC1-C/SFC160Q.Seq.d/                       1364   0.0
CFC470 (CFC470Q) /CSM/CF/CFC4-C/CFC470Q.Seq.d/                       1364   0.0
AFF450 (AFF450Q) /CSM/AF/AFF4-C/AFF450Q.Seq.d/                       1364   0.0
SFB624 (SFB624Q) /CSM/SF/SFB6-A/SFB624Q.Seq.d/                       1362   0.0
CFJ485 (CFJ485Q) /CSM/CF/CFJ4-D/CFJ485Q.Seq.d/                       1362   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL844505|AL844505.1 Plasmodium falciparum chromosome  6.          107   8e-21  2
AE017152|AE017152.1 Haemophilus ducreyi strain 35000HP section 2 of 6 of the complete genome.    74   6e-16  2
BM159195|BM159195.1 EST561718 PyBS Plasmodium yoelii yoelii cDNA clone PYCJA82 5' end, mRNA sequence.    96   1e-15  1
BX565550|BX565550.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse78b07_q1c.    66   3e-11  2
BX564426|BX564426.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse71f05_q1c.    66   3e-11  2
AX622554|AX622554.1 Sequence 5517 from Patent WO02094868.          40   1e-10  5
AX583820|AX583820.1 Sequence 197 from Patent WO02059148.           40   1e-10  5
CB098512|CB098512.1 ku54d11.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to TR:O17835 O17835 PYRUVATE KINASE ;, mRNA sequence.    64   5e-10  2
AW455356|AW455356.1 EST311894 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX10E14 5', mRNA sequence.    76   1e-09  1
E50268|E50268.1 Gene of docosahexanoic acid-producing bacterium.    58   5e-09  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RF5) RecName: Full=Pyruvate kinase;          Short=PK;      ...   442   e-123
AB050726_1(AB050726|pid:none) Toxoplasma gondii pk mRNA for pyru...   228   3e-58
AL844505_263(AL844505|pid:none) Plasmodium falciparum 3D7 chromo...   227   8e-58
AY608682_1(AY608682|pid:none) Achlya bisexualis pyruvate kinase ...   209   2e-52
AB005234_16(AB005234|pid:none) Arabidopsis thaliana genomic DNA,...   204   4e-51
AE015927_2237(AE015927|pid:none) Clostridium tetani E88, complet...   204   5e-51
FB912628_1(FB912628|pid:none) Sequence 131901 from Patent WO2008...   204   7e-51
FB912638_1(FB912638|pid:none) Sequence 131911 from Patent WO2008...   204   7e-51
EU964800_1(EU964800|pid:none) Zea mays clone 281612 pyruvate kin...   204   7e-51
FB912634_1(FB912634|pid:none) Sequence 131907 from Patent WO2008...   204   7e-51

 psg: 0.75  gvh: 0.45  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.46  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: mitochondrial
	 28.0 %: cytoplasmic
	  8.0 %: nuclear
	  4.0 %: cytoskeletal

>> prediction for CFF414 is mit

>CFF414F.Seq
AAATACATATACATATACATTAAATAATGGCCACATTATCACGTAATTTACGTTTATCAT
TAGATACACCAACCTCAACTTTTGTTAGAACCAAAATTGTTTGTACAATTGGTCCAAAAA
CAATGAGTGAAGAAGCTTTAATTAAATTAATCGAAACTGGTATGAACGTTTGTAGACTCA
ACTTTTCACATGGTACTCATGATTATCATGGTCAAGTTATTAAAAATGTTAGATCAGCAA
TGGAGAAAACAGGTAAAATTATTGCTATTATGTTAGATACTAAAGGTCCAGAAATTAGAA
CTGGTAAAATTGAAGATCGTTGTGGTTATGTTGATTTATTCGTTGGCCAAGAAATCCTCG
TTGATACCAATATGAATCAACCAGGTACTTCATTTAGAATTTCAATCGATTATAAAGGTT
TATTAGATTCAGTTAAAGTTGGTGGTTACATTTTAATTGCTGATGGTGTAATTTCATTAT
CTATTACTGCAGTTGAAAAAGAAAAAGGTCATGTTGTTTGTAGAGTT----------

>CFF414Z.Seq
----------TTCGTCGCTCAAAAGATGATCGTTAGTAAATGTAATGCCGCCGGTAAACC
AGTAATTACCGCCACTCAAATGTTGGAATCAATGATCAAGAATCCAAGACCAACCCGTGC
TGAAGCTACCGATGTTGCCAATGCCGTTTTAGATGGTTCCGATTGTGTTATGCTTTCAGG
TGAAACCGCAAGCGGTGATTACCCATACGAAGCCGTAGACATTATGGCAAAGATCTGTAG
AGAAGCAGAGTTGGTAGAATCTTCAACTGATTATCAAACTTTATTCGCTGCCCTTAAATT
ATCATCAGCCAAACCAGTCAGCATTGCTGAAACCGTTGCTTCTTACGCCGTCGCAACCGC
TATCGATTTGAAGGCTGATCTCATCATCACACTCACTGAAACCGGTCTCACTGCTCGTCT
CGTCAGTAAATATCGTCCATCCATTCCAATCATTGCCGTCACTTCATGGTCATACACTGT
CAAACATTTACTCGCCACTAGAGGTGCCATTCCATTCTTGGTAGAATCATTGGTCGGCAC
TGATAAACTCGTTGAATCTTGTCTCGAATATGCTATGAAACATAACTTATGTAAAAAAGG
TTCAAGAGTTGTCATTGTTTCTGGTGTTATGGAAGGTGTACCAGGTAAAACCAACAGTTT
AAGAGTTCTTACCGTTGGTGAATCAATCAAAGATTTAAAGAAAAAATAAAAATATAGCCA
TAATTTTAATAAGCGCAGA

>CFF414P.Seq
AAATACATATACATATACATTAAATAATGGCCACATTATCACGTAATTTACGTTTATCAT
TAGATACACCAACCTCAACTTTTGTTAGAACCAAAATTGTTTGTACAATTGGTCCAAAAA
CAATGAGTGAAGAAGCTTTAATTAAATTAATCGAAACTGGTATGAACGTTTGTAGACTCA
ACTTTTCACATGGTACTCATGATTATCATGGTCAAGTTATTAAAAATGTTAGATCAGCAA
TGGAGAAAACAGGTAAAATTATTGCTATTATGTTAGATACTAAAGGTCCAGAAATTAGAA
CTGGTAAAATTGAAGATCGTTGTGGTTATGTTGATTTATTCGTTGGCCAAGAAATCCTCG
TTGATACCAATATGAATCAACCAGGTACTTCATTTAGAATTTCAATCGATTATAAAGGTT
TATTAGATTCAGTTAAAGTTGGTGGTTACATTTTAATTGCTGATGGTGTAATTTCATTAT
CTATTACTGCAGTTGAAAAAGAAAAAGGTCATGTTGTTTGTAGAGTT----------TTC
GTCGCTCAAAAGATGATCGTTAGTAAATGTAATGCCGCCGGTAAACCAGTAATTACCGCC
ACTCAAATGTTGGAATCAATGATCAAGAATCCAAGACCAACCCGTGCTGAAGCTACCGAT
GTTGCCAATGCCGTTTTAGATGGTTCCGATTGTGTTATGCTTTCAGGTGAAACCGCAAGC
GGTGATTACCCATACGAAGCCGTAGACATTATGGCAAAGATCTGTAGAGAAGCAGAGTTG
GTAGAATCTTCAACTGATTATCAAACTTTATTCGCTGCCCTTAAATTATCATCAGCCAAA
CCAGTCAGCATTGCTGAAACCGTTGCTTCTTACGCCGTCGCAACCGCTATCGATTTGAAG
GCTGATCTCATCATCACACTCACTGAAACCGGTCTCACTGCTCGTCTCGTCAGTAAATAT
CGTCCATCCATTCCAATCATTGCCGTCACTTCATGGTCATACACTGTCAAACATTTACTC
GCCACTAGAGGTGCCATTCCATTCTTGGTAGAATCATTGGTCGGCACTGATAAACTCGTT
GAATCTTGTCTCGAATATGCTATGAAACATAACTTATGTAAAAAAGGTTCAAGAGTTGTC
ATTGTTTCTGGTGTTATGGAAGGTGTACCAGGTAAAACCAACAGTTTAAGAGTTCTTACC
GTTGGTGAATCAATCAAAGATTTAAAGAAAAAATAAAAATATAGCCATAATTTTAATAAG
CGCAGA