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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHA888
Library CH
(Link to library)
Clone ID CHA888
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1|Contig-U16524-1
Original site URL
Representative seq. ID CHA888P
(Link to Original site)
Representative DNA sequence 
>CHA888 (CHA888Q) /CSM/CH/CHA8-D/CHA888Q.Seq.d/
AGACAAAAGTAAATAAAACATGTCCGCAAGTAATAAAGATGACCAACTCATGAAAAATGA
GTTCGAAAGTACCTACGACAAAATTGTCGATTCATTCGACAATATGGAGCTCCCAAAGGA
TCTCTTAAGAGGTATCTACTCCTATGGTTTTGAAAAGCCATCAGCCATCCAACAAAGAGC
TATCATTCCACTCTCAAAAGGTTTTGATATTATTGCCCAAGCTCAATCTGGTACTGGTAA
AACTGCTACCTTCACCGTTGGTATCTTATCTAGAATTGATATTTCAGTCCTCAAATGTCA
AGCTCTCATCATTGCCCCAACTCGTGAACTTGCTCAACAAATTCAAAAGGTTGTTTTAGC
TTTAGGTGAATATTTACACATTAAATCATACGCATGTATTGGTGGTACTAAGATTACTGA
TGATATTCAAGCCCTCCAAAGTGGTGTACACGTAGTTGTCGGTACCCCAGGTCGTGTCTT
TGAAATGTTATCACGTGGTATCATTAATCGTGACACCATTAGAATTTTCTGTCTTGATGA
AGCTGATGAAATGTTATCACGTGGTTTCAAGGATCAAATCTATGAAATTTTCAGATTCTT
ACCAAAGAXXXXXXXXXXCCCCCCCCNTCNCGNCCAATGTGTGNTGNATCATTCNCTGNA
TACCCNCCNTTAGGTCGTTTCGCCGNCAGAGATATGAGACAAACCGTTGCCGNCGGNGNC
ATCAAATCNACCGTCAAGAAAGCCCCAGGTAAAGCNGGTGATAAGAAAGGGGCCGCNGCC
CCATCAAAGGAAGCAATATTNATTATTTTAAAAAAAATTTTATT
sequence update 2002.10.25
Translated Amino Acid sequence 
dksk*NMSASNKDDQLMKNEFESTYDKIVDSFDNMELPKDLLRGIYSYGFEKPSAIQQRA
IIPLSKGFDIIAQAQSGTGKTATFTVGILSRIDISVLKCQALIIAPTRELAQQIQKVVLA
LGEYLHIKSYACIGGTKITDDIQALQSGVHVVVGTPGRVFEMLSRGIINRDTIRIFCLDE
ADEMLSRGFKDQIYEIFRFLPK---

---PPPSRPMCXXSFXXYPPLGRFAXRDMRQTVAXGXIKSTVKKAPGKAGDKKGAAAPSK
EAIXIILKKIL
Translated Amino Acid sequence (All Frames) 
Frame A:
rqk*ikhvrk**r*pthek*vrkylrqncrfirqygapkgslkrylllwf*kaishptks
yhstlkrf*yycpssiwyw*ncylhrwyli*n*yfspqmssshhcpns*tcstnskgcfs
fr*ifth*iirmywwy*dy**yssppkwctrscryprscl*nvitwyh*s*hh*nfls**
s**nvitwfqgsnl*nfqiltk---

---PPPSRPMCXXSFXXYPPLGRFAXRDMRQTVAXGXIKSTVKKAPGKAGDKKGAAAPSK
EAIXIILKKIL

Frame B:
dksk*NMSASNKDDQLMKNEFESTYDKIVDSFDNMELPKDLLRGIYSYGFEKPSAIQQRA
IIPLSKGFDIIAQAQSGTGKTATFTVGILSRIDISVLKCQALIIAPTRELAQQIQKVVLA
LGEYLHIKSYACIGGTKITDDIQALQSGVHVVVGTPGRVFEMLSRGIINRDTIRIFCLDE
ADEMLSRGFKDQIYEIFRFLPK---

---ppxxxqcvxxhslxtxx*vvspxei*dkplpxxxsnxpsrkpqvkxvirkgpxphqr
kqyxlf*kkfy

Frame C:
tkvnktcpqvikmtns*kmsskvpttklsihstiwssqris*evstpmvlkshqpsnkel
sfhsqkvlillpklnlvlvkllpsplvsylelifqssnvklsslpqlvnllnkfkrlf*l
*vniytlnhthvlvvlrllmifkpskvvyt*lsvpqvvslkcyhvvslivtplefsvlmk
lmkcyhvvsriksmkfsdsyqr---

---ppxxxnvxxiixxipxxrsfrrqryetnrcrrxhqixrqespr*sx**ergrxpikg
snixyfkknfi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN391 (VFN391Q) /CSM/VF/VFN3-D/VFN391Q.Seq.d/                       1205   0.0
VFA173 (VFA173Q) /CSM/VF/VFA1-D/VFA173Q.Seq.d/                       1205   0.0
SFK579 (SFK579Q) /CSM/SF/SFK5-D/SFK579Q.Seq.d/                       1205   0.0
SFJ213 (SFJ213Q) /CSM/SF/SFJ2-A/SFJ213Q.Seq.d/                       1205   0.0
SFF340 (SFF340Q) /CSM/SF/SFF3-B/SFF340Q.Seq.d/                       1205   0.0
CHC226 (CHC226Q) /CSM/CH/CHC2-B/CHC226Q.Seq.d/                       1205   0.0
CHA888 (CHA888Q) /CSM/CH/CHA8-D/CHA888Q.Seq.d/                       1205   0.0
CFJ156 (CFJ156Q) /CSM/CF/CFJ1-C/CFJ156Q.Seq.d/                       1205   0.0
SFK631 (SFK631Q) /CSM/SF/SFK6-B/SFK631Q.Seq.d/                       1201   0.0
CFH766 (CFH766Q) /CSM/CF/CFH7-C/CFH766Q.Seq.d/                       1199   0.0
own update 2002.11.20
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U78759|U78759.1 Dictyostelium discoideum IfdA (ifdA) mRNA, partial cds.  1170   0.0    1
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.   208   1e-49  1
X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.   192   6e-45  1
AU011976|AU011976.1 Schizosaccharomyces pombe cDNA, clone spc06170.    66   6e-41  5
AU009453|AU009453.1 Schizosaccharomyces pombe cDNA, clone spc04934.    66   4e-39  5
X80796|X80796.1 S.pombe eIF-4A mRNA for translation initiation factor.    66   5e-39  5
L40627|L40627.1 Schizosaccharomyces pombe cell cycle control protein eIF-4A (tif1) mRNA, complete cds.    66   5e-39  5
AU011975|AU011975.1 Schizosaccharomyces pombe cDNA, clone spc06169.    66   5e-35  4
AL132828|AL132828.1 S.pombe chromosome I cosmid c1006.             66   3e-34  5
AU011946|AU011946.1 Schizosaccharomyces pombe cDNA, clone spc06139.    66   5e-34  5
dna update 2004. 9.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U78759_1(U78759|pid:none) Dictyostelium discoideum IfdA (ifdA) m...   384   e-105
CP001142_274(CP001142|pid:none) Phaeodactylum tricornutum CCAP 1...   261   1e-68
(Q9CAI7) RecName: Full=Eukaryotic initiation factor 4A-3;       ...   258   2e-67
S52018(S52018;S44928)translation initiation factor eIF-4A.11 - c...   254   2e-66
(P41382) RecName: Full=Eukaryotic initiation factor 4A-10;      ...   254   3e-66
(Q40466) RecName: Full=Eukaryotic initiation factor 4A-13;      ...   254   3e-66
S52019(S52019;S44933)translation initiation factor eIF-4A.7 - co...   254   3e-66
(Q40470) RecName: Full=Eukaryotic initiation factor 4A-7;       ...   254   3e-66
(P41379) RecName: Full=Eukaryotic initiation factor 4A-2;       ...   254   3e-66
(Q40465) RecName: Full=Eukaryotic initiation factor 4A-11;      ...   253   4e-66
protein update 2009. 3.29
PSORT 

 psg: 0.75  gvh: 0.28  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for CHA888 is cyt
5' end seq. ID CHA888F
5' end seq. 
>CHA888F.Seq
AGACAAAAGTAAATAAAACATGTCCGCAAGTAATAAAGATGACCAACTCATGAAAAATGA
GTTCGAAAGTACCTACGACAAAATTGTCGATTCATTCGACAATATGGAGCTCCCAAAGGA
TCTCTTAAGAGGTATCTACTCCTATGGTTTTGAAAAGCCATCAGCCATCCAACAAAGAGC
TATCATTCCACTCTCAAAAGGTTTTGATATTATTGCCCAAGCTCAATCTGGTACTGGTAA
AACTGCTACCTTCACCGTTGGTATCTTATCTAGAATTGATATTTCAGTCCTCAAATGTCA
AGCTCTCATCATTGCCCCAACTCGTGAACTTGCTCAACAAATTCAAAAGGTTGTTTTAGC
TTTAGGTGAATATTTACACATTAAATCATACGCATGTATTGGTGGTACTAAGATTACTGA
TGATATTCAAGCCCTCCAAAGTGGTGTACACGTAGTTGTCGGTACCCCAGGTCGTGTCTT
TGAAATGTTATCACGTGGTATCATTAATCGTGACACCATTAGAATTTTCTGTCTTGATGA
AGCTGATGAAATGTTATCACGTGGTTTCAAGGATCAAATCTATGAAATTTTCAGATTCTT
ACCAAAGANNNNNNNNNN
Length of 5' end seq. 618
3' end seq. ID CHA888Z
3' end seq. 
>CHA888Z.Seq
NNNNNNNNNNCCCCCCCCNTCNCGNCCAATGTGTGNTGNATCATTCNCTGNATACCCNCC
NTTAGGTCGTTTCGCCGNCAGAGATATGAGACAAACCGTTGCCGNCGGNGNCATCAAATC
NACCGTCAAGAAAGCCCCAGGTAAAGCNGGTGATAAGAAAGGGGCCGCNGCCCCATCAAA
GGAAGCAATATTNATTATTTTAAAAAAAATTTTATT
Length of 3' end seq. 216
Connected seq. ID CHA888P
Connected seq. 
>CHA888P.Seq
AGACAAAAGTAAATAAAACATGTCCGCAAGTAATAAAGATGACCAACTCATGAAAAATGA
GTTCGAAAGTACCTACGACAAAATTGTCGATTCATTCGACAATATGGAGCTCCCAAAGGA
TCTCTTAAGAGGTATCTACTCCTATGGTTTTGAAAAGCCATCAGCCATCCAACAAAGAGC
TATCATTCCACTCTCAAAAGGTTTTGATATTATTGCCCAAGCTCAATCTGGTACTGGTAA
AACTGCTACCTTCACCGTTGGTATCTTATCTAGAATTGATATTTCAGTCCTCAAATGTCA
AGCTCTCATCATTGCCCCAACTCGTGAACTTGCTCAACAAATTCAAAAGGTTGTTTTAGC
TTTAGGTGAATATTTACACATTAAATCATACGCATGTATTGGTGGTACTAAGATTACTGA
TGATATTCAAGCCCTCCAAAGTGGTGTACACGTAGTTGTCGGTACCCCAGGTCGTGTCTT
TGAAATGTTATCACGTGGTATCATTAATCGTGACACCATTAGAATTTTCTGTCTTGATGA
AGCTGATGAAATGTTATCACGTGGTTTCAAGGATCAAATCTATGAAATTTTCAGATTCTT
ACCAAAGA----------CCCCCCCCNTCNCGNCCAATGTGTGNTGNATCATTCNCTGNA
TACCCNCCNTTAGGTCGTTTCGCCGNCAGAGATATGAGACAAACCGTTGCCGNCGGNGNC
ATCAAATCNACCGTCAAGAAAGCCCCAGGTAAAGCNGGTGATAAGAAAGGGGCCGCNGCC
CCATCAAAGGAAGCAATATTNATTATTTTAAAAAAAATTTTATT
Length of connected seq. 814
Full length Seq ID -
Full length Seq. -
Length of full length seq. -