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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFG354
Library AF
(Link to library)
Clone ID AFG354
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFG354P
(Link to Original site)
Representative DNA sequence 
>AFG354 (AFG354Q) /CSM/AF/AFG3-C/AFG354Q.Seq.d/
AAAAAAGACAATAATTATTAATAAGAATGGTTTTACAATTTGTCGACGATTCATCATGTA
AATCAGCAATTGCAGATTTAAGAAATGATTCAACCTCAACTGATTGGGTATTATTATCAT
TTGAATCACCAAAATCAGAAAAGATTAAATTAGTTGGAAGTGGTTCAGGTGGTGTAGCAG
AATTAGTTGAACATTTACAAGATGATTTGGTTGGATTTGCATTGGTTAGAAAGATTGATA
AAATCGATGATAGTGAAACCGTTAAATTTGCATTCATCAATTTCATTGGAGAAAAAGTTG
GAATCTTTCAAAAAGGTAAAATCTCTGTCACCATTGGTGGTGTTAAAGATTTCTTGGGTT
CATTCCAATGTGATTTCTTAATCACCTCAAAGAATGAAATCTCTGATGAAATCGTATTAA
ATAAAATTCAAGATATCTCTGGTACAGGTTCAAAAGTTTTAGATGAAAATGGTGCAAAAA
CTGGTGCTTCAAAATCAACCACTTCACCAACCAACTCTTTCAGAAAATCAACATACGGTT
CAGTTTCTTCACCATCATCCTCAAATAAAGACTCAATTAAATTAGGTGATGAACCAGCAA
TTCGTGAAGCCTTAAAAGAATTTAGATCCGATGAAAATGATATCGATTGGGTACTCTXXX
XXXXXXXTGTTAAGAGCACGTTTAGGTACTCATACTGGATTTGTAAATCAGTTAGTTACA
CCATATCATGTTGATATTAAATGTACAGTTAAATCAGAAATTTCAAAAGAGATTGTAGAG
GAAACCGTTGCAAAAGCATCAGGTAAAGCATCAATGGTATTGAGTGATGATGCTCAATCA
CGTGGTAGTGGTGGTCGTATGACCTCAAGTTACAGTTCATCAAGTGCTTCAACTCAAGGT
TCAAACACAACCGGTAAAACATCTGCTGGTGCTGGTAGTAAATTTACAACACCATCAGCT
GTGCCAAAATCAAGTGGTTCAACACTTGGTTTCGATGATGAACAACAAGTTAGAGATACA
ATCAAAGATGTTAGATCCGATAGTACTGACACTAATTGGTGTTTAGTTGGTTATAAAAAT
GATACCACTTTAACTGTCATTGCTAAAGGAAATGGTGGTGTTGACGAATTGGTCGGCCAT
TTGAATTCAAAGATCGTTGCTTATGGCTTAGTTCGTGAAGTTGAAAGATTCGATCTCTCT
GATACCATCAAATTTGCTTTCATCGATTTCGTTGGTGAAGATATCAATCGTATGTTTAGA
GCTAAACTTGGTACTCATTCTGGTACTGTTAAACAATTATTCCATCCATATCATGTTGAT
TTACATGTCTCCTCTCCTTCTGAATTATCCTCTGAAATCGTTACTAAAACTATCACTCAA
AATTCGGAACTTCTTCAAGA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
krq*LLIRMVLQFVDDSSCKSAIADLRNDSTSTDWVLLSFESPKSEKIKLVGSGSGGVAE
LVEHLQDDLVGFALVRKIDKIDDSETVKFAFINFIGEKVGIFQKGKISVTIGGVKDFLGS
FQCDFLITSKNEISDEIVLNKIQDISGTGSKVLDENGAKTGASKSTTSPTNSFRKSTYGS
VSSPSSSNKDSIKLGDEPAIREALKEFRSDENDIDWVL---

---LRARLGTHTGFVNQLVTPYHVDIKCTVKSEISKEIVEETVAKASGKASMVLSDDAQS
RGSGGRMTSSYSSSSASTQGSNTTGKTSAGAGSKFTTPSAVPKSSGSTLGFDDEQQVRDT
IKDVRSDSTDTNWCLVGYKNDTTLTVIAKGNGGVDELVGHLNSKIVAYGLVREVERFDLS
DTIKFAFIDFVGEDINRMFRAKLGTHSGTVKQLFHPYHVDLHVSSPSELSSEIVTKTITQ
NSELLQ
Translated Amino Acid sequence (All Frames) 
Frame A:
kkdnny**ewfynlstihhvnqqlqi*emiqpqligyyyhlnhqnqkrln*levvqvv*q
n*lniykmiwldlhwlerliksmivkplnlhssislekklesfkkvkslsplvvlkiswv
hsnvis*spqrmkslmksy*ikfkislvqvqkf*mkmvqklvlqnqplhqptlsenqhtv
qflhhhpqiktqln*vmnqqfvkp*knldpmkmisigys---

---c*ehv*vlildl*is*lhhimlilnvqlnqkfqkrl*rkplqkhqvkhqwy*vmmln
hvvvvvv*pqvtvhqvlqlkvqtqpvkhllvlvvnlqhhqlcqnqvvqhlvsmmnnklei
qskmldpivltligv*lvikmipl*lsllkemvvltnwsai*iqrsllma*fvklkdsis
lipsnllssislvkisivclelnlvlilvllnnysihimliymsplllnyplksllklsl
kirnffk

Frame B:
kktiiinkngfticrrfim*isncrfkk*fnln*lgiiii*itkirkd*iswkwfrwcsr
is*tftr*fgwicig*kd**nr***nr*icihqfhwrkswnlskr*nlchhwwc*rflgf
ipm*flnhlke*nl**nrik*nsrylwyrfksfr*kwcknwcfkinhftnqlfqkinirf
sfftiilk*rln*ir**tsns*slkri*ir*k*yrlgt---

---vkstfrysywicksvsytisc*y*mys*irnfkrdcrgnrcksir*singie**csi
tw*wwsydlklqfikcfnsrfkhnr*nicwcw**iyntiscakikwfntwfr**tts*ry
nqrc*ir*y*h*lvfswl*k*yhfnchc*rkwwc*rigrpfefkdrclwlss*s*kirsl
*yhqicfhrfrw*ryqsyv*s*twysfwyc*tiipsisc*ftcllsf*iil*nry*nyhs
kfgtssr

Frame C:
krq*LLIRMVLQFVDDSSCKSAIADLRNDSTSTDWVLLSFESPKSEKIKLVGSGSGGVAE
LVEHLQDDLVGFALVRKIDKIDDSETVKFAFINFIGEKVGIFQKGKISVTIGGVKDFLGS
FQCDFLITSKNEISDEIVLNKIQDISGTGSKVLDENGAKTGASKSTTSPTNSFRKSTYGS
VSSPSSSNKDSIKLGDEPAIREALKEFRSDENDIDWVL---

---LRARLGTHTGFVNQLVTPYHVDIKCTVKSEISKEIVEETVAKASGKASMVLSDDAQS
RGSGGRMTSSYSSSSASTQGSNTTGKTSAGAGSKFTTPSAVPKSSGSTLGFDDEQQVRDT
IKDVRSDSTDTNWCLVGYKNDTTLTVIAKGNGGVDELVGHLNSKIVAYGLVREVERFDLS
DTIKFAFIDFVGEDINRMFRAKLGTHSGTVKQLFHPYHVDLHVSSPSELSSEIVTKTITQ
NSELLQ
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFG354 (AFG354Q) /CSM/AF/AFG3-C/AFG354Q.Seq.d/                       2716   0.0
AFO410 (AFO410Q) /CSM/AF/AFO4-A/AFO410Q.Seq.d/                       1437   0.0
CFC730 (CFC730Q) /CSM/CF/CFC7-B/CFC730Q.Seq.d/                       1431   0.0
CFJ633 (CFJ633Q) /CSM/CF/CFJ6-B/CFJ633Q.Seq.d/                       1429   0.0
CHB189 (CHB189Q) /CSM/CH/CHB1-D/CHB189Q.Seq.d/                       1425   0.0
CFC752 (CFC752Q) /CSM/CF/CFC7-C/CFC752Q.Seq.d/                       1425   0.0
CHB716 (CHB716Q) /CSM/CH/CHB7-A/CHB716Q.Seq.d/                       1423   0.0
SFH162 (SFH162Q) /CSM/SF/SFH1-C/SFH162Q.Seq.d/                       1419   0.0
CFE535 (CFE535Q) /CSM/CF/CFE5-B/CFE535Q.Seq.d/                       1415   0.0
CFC305 (CFC305Q) /CSM/CF/CFC3-A/CFC305Q.Seq.d/                       1409   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116922|AC116922.2 Dictyostelium discoideum chromosome 2 map 6061442-6097630 strain AX4, complete sequence.  1407   0.0    2
AL445565|AL445565.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 3/3.    46   5e-04  13
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    32   0.002  19
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    32   0.014  21
AR300198|AR300198.1 Sequence 1 from patent US 6537773.             36   0.030  21
AC117076|AC117076.3 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.    34   0.031  14
BX842642|BX842642.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 1/4.    34   0.063  19
CC067446|CC067446.1 CSU-K33r.11J23.SP6 CSU-K33r Aedes aegypti genomic clone CSU-K33r.11J23, DNA sequence.    50   0.090  1
AQ651762|AQ651762.1 Sheared DNA-20H15.TR Sheared DNA Trypanosoma brucei genomic clone Sheared DNA-20H15, DNA sequence.    50   0.090  1
BQ916725|BQ916725.1 QHB18N09.yg.ab1 QH_ABCDI sunflower RHA801 Helianthus annuus cDNA clone QHB18N09, mRNA sequence.    40   0.11   2
dna update 2004. 5. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116922_4(AC116922|pid:none) Dictyostelium discoideum chromosom...   411   e-113
AE017342_92(AE017342|pid:none) Cryptococcus neoformans var. neof...    64   1e-08
(A6H7G2) RecName: Full=Drebrin-like protein;  &BC146234_1(BC1462...    63   3e-08
(Q6GM14) RecName: Full=Drebrin-like protein B;  &BC074277_1(BC07...    62   4e-08
(Q7ZXQ9) RecName: Full=Drebrin-like protein A;  &BC044296_1(BC04...    62   5e-08
CR457167_1(CR457167|pid:none) Homo sapiens full open reading fra...    62   6e-08
AB170106_1(AB170106|pid:none) Macaca fascicularis brain cDNA clo...    62   6e-08
AY122215_1(AY122215|pid:none) Drosophila melanogaster LP08941 fu...    62   6e-08
BC011677_1(BC011677|pid:none) Homo sapiens drebrin-like, mRNA (c...    62   6e-08
(Q9UJU6) RecName: Full=Drebrin-like protein; AltName: Full=Drebr...    62   6e-08
protein update 2009. 4.20
PSORT 

 psg: 0.66  gvh: 0.59  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for AFG354 is cyt
5' end seq. ID AFG354F
5' end seq. 
>AFG354F.Seq
AAAAAAGACAATAATTATTAATAAGAATGGTTTTACAATTTGTCGACGATTCATCATGTA
AATCAGCAATTGCAGATTTAAGAAATGATTCAACCTCAACTGATTGGGTATTATTATCAT
TTGAATCACCAAAATCAGAAAAGATTAAATTAGTTGGAAGTGGTTCAGGTGGTGTAGCAG
AATTAGTTGAACATTTACAAGATGATTTGGTTGGATTTGCATTGGTTAGAAAGATTGATA
AAATCGATGATAGTGAAACCGTTAAATTTGCATTCATCAATTTCATTGGAGAAAAAGTTG
GAATCTTTCAAAAAGGTAAAATCTCTGTCACCATTGGTGGTGTTAAAGATTTCTTGGGTT
CATTCCAATGTGATTTCTTAATCACCTCAAAGAATGAAATCTCTGATGAAATCGTATTAA
ATAAAATTCAAGATATCTCTGGTACAGGTTCAAAAGTTTTAGATGAAAATGGTGCAAAAA
CTGGTGCTTCAAAATCAACCACTTCACCAACCAACTCTTTCAGAAAATCAACATACGGTT
CAGTTTCTTCACCATCATCCTCAAATAAAGACTCAATTAAATTAGGTGATGAACCAGCAA
TTCGTGAAGCCTTAAAAGAATTTAGATCCGATGAAAATGATATCGATTGGGTACTCT---
-------
Length of 5' end seq. 657
3' end seq. ID AFG354Z
3' end seq. 
>AFG354Z.Seq
----------TGTTAAGAGCACGTTTAGGTACTCATACTGGATTTGTAAATCAGTTAGTT
ACACCATATCATGTTGATATTAAATGTACAGTTAAATCAGAAATTTCAAAAGAGATTGTA
GAGGAAACCGTTGCAAAAGCATCAGGTAAAGCATCAATGGTATTGAGTGATGATGCTCAA
TCACGTGGTAGTGGTGGTCGTATGACCTCAAGTTACAGTTCATCAAGTGCTTCAACTCAA
GGTTCAAACACAACCGGTAAAACATCTGCTGGTGCTGGTAGTAAATTTACAACACCATCA
GCTGTGCCAAAATCAAGTGGTTCAACACTTGGTTTCGATGATGAACAACAAGTTAGAGAT
ACAATCAAAGATGTTAGATCCGATAGTACTGACACTAATTGGTGTTTAGTTGGTTATAAA
AATGATACCACTTTAACTGTCATTGCTAAAGGAAATGGTGGTGTTGACGAATTGGTCGGC
CATTTGAATTCAAAGATCGTTGCTTATGGCTTAGTTCGTGAAGTTGAAAGATTCGATCTC
TCTGATACCATCAAATTTGCTTTCATCGATTTCGTTGGTGAAGATATCAATCGTATGTTT
AGAGCTAAACTTGGTACTCATTCTGGTACTGTTAAACAATTATTCCATCCATATCATGTT
GATTTACATGTCTCCTCTCCTTCTGAATTATCCTCTGAAATCGTTACTAAAACTATCACT
CAAAATTCGGAACTTCTTCAAGA
Length of 3' end seq. 733
Connected seq. ID AFG354P
Connected seq. 
>AFG354P.Seq
AAAAAAGACAATAATTATTAATAAGAATGGTTTTACAATTTGTCGACGATTCATCATGTA
AATCAGCAATTGCAGATTTAAGAAATGATTCAACCTCAACTGATTGGGTATTATTATCAT
TTGAATCACCAAAATCAGAAAAGATTAAATTAGTTGGAAGTGGTTCAGGTGGTGTAGCAG
AATTAGTTGAACATTTACAAGATGATTTGGTTGGATTTGCATTGGTTAGAAAGATTGATA
AAATCGATGATAGTGAAACCGTTAAATTTGCATTCATCAATTTCATTGGAGAAAAAGTTG
GAATCTTTCAAAAAGGTAAAATCTCTGTCACCATTGGTGGTGTTAAAGATTTCTTGGGTT
CATTCCAATGTGATTTCTTAATCACCTCAAAGAATGAAATCTCTGATGAAATCGTATTAA
ATAAAATTCAAGATATCTCTGGTACAGGTTCAAAAGTTTTAGATGAAAATGGTGCAAAAA
CTGGTGCTTCAAAATCAACCACTTCACCAACCAACTCTTTCAGAAAATCAACATACGGTT
CAGTTTCTTCACCATCATCCTCAAATAAAGACTCAATTAAATTAGGTGATGAACCAGCAA
TTCGTGAAGCCTTAAAAGAATTTAGATCCGATGAAAATGATATCGATTGGGTACTCT---
-------TGTTAAGAGCACGTTTAGGTACTCATACTGGATTTGTAAATCAGTTAGTTACA
CCATATCATGTTGATATTAAATGTACAGTTAAATCAGAAATTTCAAAAGAGATTGTAGAG
GAAACCGTTGCAAAAGCATCAGGTAAAGCATCAATGGTATTGAGTGATGATGCTCAATCA
CGTGGTAGTGGTGGTCGTATGACCTCAAGTTACAGTTCATCAAGTGCTTCAACTCAAGGT
TCAAACACAACCGGTAAAACATCTGCTGGTGCTGGTAGTAAATTTACAACACCATCAGCT
GTGCCAAAATCAAGTGGTTCAACACTTGGTTTCGATGATGAACAACAAGTTAGAGATACA
ATCAAAGATGTTAGATCCGATAGTACTGACACTAATTGGTGTTTAGTTGGTTATAAAAAT
GATACCACTTTAACTGTCATTGCTAAAGGAAATGGTGGTGTTGACGAATTGGTCGGCCAT
TTGAATTCAAAGATCGTTGCTTATGGCTTAGTTCGTGAAGTTGAAAGATTCGATCTCTCT
GATACCATCAAATTTGCTTTCATCGATTTCGTTGGTGAAGATATCAATCGTATGTTTAGA
GCTAAACTTGGTACTCATTCTGGTACTGTTAAACAATTATTCCATCCATATCATGTTGAT
TTACATGTCTCCTCTCCTTCTGAATTATCCTCTGAAATCGTTACTAAAACTATCACTCAA
AATTCGGAACTTCTTCAAGA
Length of connected seq. 1390
Full length Seq ID -
Full length Seq. -
Length of full length seq. -