>AFG542 (AFG542Q) /CSM/AF/AFG5-B/AFG542Q.Seq.d/
ATTCATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATTATATATATATATAAAAA
TATAAAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTATTTTTAATTACATTA
TATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAGTCATCGTTCAGAA
TATACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTATCATGTTGTGGTAAG
AAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGATTTATCATGTGCT
AAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAAACCACATAGAGAT
TTTGTACCATATCATTCACCAAATACAACCACATGTGAATCATTAGGTTGTGAAGCAAGA
GGTATGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACTTCTTGTTGCCCAAGA
ATTCCAAGATGTGTTGGTCATCATGGTGGCGGTCATAAATGTGACAGAATGAGATGTCCA
GAAGGATTCTAXXXXXXXXXX

ihsil*l*kkesnnyiyi*KYKKNKMRILYLASLLFLITLYLSPTFGWGGGRDCESHRSE
YTCKSDRSCAYLPFVSCCGKKEFFCVNRDHRNCCDDLSCAKNTRTGEIFEIWSSCKPHRD
FVPYHSPNTTTCESLGCEARGMECEWVESSPCYGTSCCPRIPRCVGHHGGGHKCDRMRCP
EGF---

Frame A:
ihsil*l*kkesnnyiyi*KYKKNKMRILYLASLLFLITLYLSPTFGWGGGRDCESHRSE
YTCKSDRSCAYLPFVSCCGKKEFFCVNRDHRNCCDDLSCAKNTRTGEIFEIWSSCKPHRD
FVPYHSPNTTTCESLGCEARGMECEWVESSPCYGTSCCPRIPRCVGHHGGGHKCDRMRCP
EGF---


Frame B:
fiqfcnykkrnqiiiyiyknikkik*efyi*llfyf*lhyiyhqllvgvvaeivkvivqn
ihvnqievvhiyhlyhvvvrknfsvliviieivvmiyhvlktrelvkslrfgqvvnhiei
lyhiihqiqphvnh*vvkqevwnvnglnhhhvmellvaqefqdvlvimvavinvte*dvq
kds---


Frame C:
sfnfviikkgik*lyiyiki*kk*nenfifsfsfifnyiisitnfwlgwwqrl*kssfri
ym*ir*klcifticimlw*eriflc*s*s*kll**fimc*khenw*nl*dlvkl*tt*rf
ctisftkynhm*iirl*skrygm*mg*iitmlwnfllpknskmcwsswwrs*m*qnemsr
ri---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLG851 (SLG851Q) /CSM/SL/SLG8-C/SLG851Q.Seq.d/                        952   0.0
SLB538 (SLB538Q) /CSM/SL/SLB5-B/SLB538Q.Seq.d/                        952   0.0
SLA438 (SLA438Q) /CSM/SL/SLA4-B/SLA438Q.Seq.d/                        952   0.0
SLA432 (SLA432Q) /CSM/SL/SLA4-B/SLA432Q.Seq.d/                        952   0.0
SLA402 (SLA402Q) /CSM/SL/SLA4-A/SLA402Q.Seq.d/                        952   0.0
SHH249 (SHH249Q) /CSM/SH/SHH2-C/SHH249Q.Seq.d/                        952   0.0
SHF542 (SHF542Q) /CSM/SH/SHF5-B/SHF542Q.Seq.d/                        952   0.0
SHD608 (SHD608Q) /CSM/SH/SHD6-A/SHD608Q.Seq.d/                        952   0.0
SHA862 (SHA862Q) /CSM/SH/SHA8-C/SHA862Q.Seq.d/                        952   0.0
SFL361 (SFL361Q) /CSM/SF/SFL3-C/SFL361Q.Seq.d/                        952   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

D13973|D13973.1 Dictyostelium discoideum DNA for Dp87 protein, complete cds.   777   0.0    3
AC073320|AC073320.8 Homo sapiens clone RP11-306G20, complete sequence.    48   0.14   1
AC145891|AC145891.2 Pan troglodytes chromosome 7 clone RP43-20A24, WORKING DRAFT SEQUENCE, 13 unordered pieces.    48   0.14   1
AC145984|AC145984.2 Pan troglodytes chromosome 7 clone RP43-17F10, WORKING DRAFT SEQUENCE, 5 unordered pieces.    48   0.14   1
AC084865|AC084865.3 Homo sapiens chromosome 7 clone RP11-437L1, complete sequence.    48   0.14   1
AC079746|AC079746.2 Homo sapiens chromosome 7 clone CTD-2353O2, WORKING DRAFT SEQUENCE, 19 unordered pieces.    48   0.14   1
AZ208391|AZ208391.1 SP_0150_A1_B06_T7A Strongylocentrotus purpuratus, purple sea urchin , sperm genomic BAC library Strongylocentrotus purpuratus genomic clone Plate=150 Col=11 Row=C, DNA sequence.    48   0.14   1
BG603433|BG603433.1 EST502523 Plasmodium yoelii sporozoite cDNA Plasmodium yoelii cDNA clone PYCDR54, mRNA sequence.    38   0.22   2
BG603400|BG603400.1 EST502490 Plasmodium yoelii sporozoite cDNA Plasmodium yoelii cDNA clone PYCDR24, mRNA sequence.    38   0.24   2
AE014847|AE014847.1 Plasmodium falciparum 3D7 chromosome 12, section 4 of 9 of the complete sequence.    32   0.34   7

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       362   4e-99
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   358   4e-98
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...   136   3e-31
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...   102   7e-21
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    64   3e-09
B25439(B25439)Beejin protein - slime mold (Dictyostelium discoid...    62   1e-08
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...    53   5e-06
AC117076_50(AC117076|pid:none) Dictyostelium discoideum chromoso...    52   1e-05
AC117076_49(AC117076|pid:none) Dictyostelium discoideum chromoso...    47   3e-04
FJ501956_1(FJ501956|pid:none) Oryza sativa Indica Group keratin ...    40   0.040

 psg: 0.96  gvh: 0.77  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.32  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for AFG542 is exc

>AFG542F.Seq
ATTCATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATTATATATATATATAAAAA
TATAAAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTATTTTTAATTACATTA
TATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAGTCATCGTTCAGAA
TATACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTATCATGTTGTGGTAAG
AAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGATTTATCATGTGCT
AAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAAACCACATAGAGAT
TTTGTACCATATCATTCACCAAATACAACCACATGTGAATCATTAGGTTGTGAAGCAAGA
GGTATGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACTTCTTGTTGCCCAAGA
ATTCCAAGATGTGTTGGTCATCATGGTGGCGGTCATAAATGTGACAGAATGAGATGTCCA
GAAGGATTCTA----------