>AFG841 (AFG841Q) /CSM/AF/AFG8-B/AFG841Q.Seq.d/
XXXXXXXXXXGAANCCATCGCCAAAGGTGGTGAACCAGCTTGGAAAGTTTCCATTCAATT
GATGGAATTTGAAGATGCCTTAAAGTATCGTTTCAATCCATTCGATGTAACAAAGATTTG
GTCACACAAAGACTATCCATTAATTCAAATTGGTCGTATGGTTTTGAATCGTAATCCAGA
GAATTACTTTGCTGAAGTTGAACAAGCTGCCTTCTCACCATCCCACATGGTTCCAGGTAT
TGAACCATCACCAGATAAGATGTTACAAGGTCGTCTCTTCTCTTATCCAGATACTCATCG
TCATCGTTTAGGTGTAAACTATCAACAAATCCCAGTCAATTGTCCATTCGCCGTAAAGGG
TGGTGTTAAAAACTACCAACGTGATGGTTTCATGGCTGTCAATGGTAATGGTGGTAAAGG
TCCAAACTATCAACCAAACTCTTTCGGTGGTCCAGAGCCACATCCAGAATTTGCTCAACA
TAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAACCATACAATCATCCAAATGACGACTT
TGTTCAACCAGGTGATTTATATAGACTCATGTCTGAAGATGCCAAATCTCGTTTCGTCTC
TAATTTAGTCGGTCACATGTCCGGTGTCACCATCAAAGAAATTCAAGTTCGTGCCGTTTC
AAACTTTTACAAGGCTGACAAAGATTTAGGTGCTCGTTTATGTAAGGGTCTTGGTATTGA
TGTTAATNATGTCATCAAATTCGCTGCTAGATCAAACTTGTAAATCAATATATTAAAAA

---XIAKGGEPAWKVSIQLMEFEDALKYRFNPFDVTKIWSHKDYPLIQIGRMVLNRNPEN
YFAEVEQAAFSPSHMVPGIEPSPDKMLQGRLFSYPDTHRHRLGVNYQQIPVNCPFAVKGG
VKNYQRDGFMAVNGNGGKGPNYQPNSFGGPEPHPEFAQHKFDVSGFAARQPYNHPNDDFV
QPGDLYRLMSEDAKSRFVSNLVGHMSGVTIKEIQVRAVSNFYKADKDLGARLCKGLGIDV
NXVIKFAARSNL*inilk

Frame A:
---xpspkvvnqlgkfpfn*wnlkmp*sivsihsm*qrfghtktih*fklvvwf*iviqr
itllklnklpshhptwfqvlnhhqircykvvssliqiliviv*v*tinksqsivhsp*rv
vlkttnvmvswlsmvmvvkvqtinqtlsvvqshiqnllninltslvsllvnhtiiqmttl
fnqviyidsclkmpnlvssli*svtcpvspskkfkfvpfqtftrltki*vlvyvrvlvlm
lxmssnslldqtcksiy*k


Frame B:
---xhrqrw*tslesfhsidgi*rclkvsfqsircnkdlvtqrlsinsnwsygfes*sre
llc*s*tsclltiphgsry*titr*dvtrsslllsryssssfrcklstnpsqlsirrkgw
c*klpt*wfhgcqw*ww*rsklstklfrwsratsricst*i*rlwfrcsstiqssk*rlc
str*fi*thv*rcqisfrl*fsrshvrchhqrnssscrfkllqg*qrfrcsfm*gswy*c
*xchqirc*iklvnqyik


Frame C:
---XIAKGGEPAWKVSIQLMEFEDALKYRFNPFDVTKIWSHKDYPLIQIGRMVLNRNPEN
YFAEVEQAAFSPSHMVPGIEPSPDKMLQGRLFSYPDTHRHRLGVNYQQIPVNCPFAVKGG
VKNYQRDGFMAVNGNGGKGPNYQPNSFGGPEPHPEFAQHKFDVSGFAARQPYNHPNDDFV
QPGDLYRLMSEDAKSRFVSNLVGHMSGVTIKEIQVRAVSNFYKADKDLGARLCKGLGIDV
NXVIKFAARSNL*inilk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FC-142 (FC-142Q) /CSM/CG/CG-FC/FC-142Q.Seq.d/                        1513   0.0
AFG841 (AFG841Q) /CSM/AF/AFG8-B/AFG841Q.Seq.d/                       1513   0.0
AFE580 (AFE580Q) /CSM/AF/AFE5-D/AFE580Q.Seq.d/                       1505   0.0
CFI568 (CFI568Q) /CSM/CF/CFI5-C/CFI568Q.Seq.d/                       1483   0.0
AFI254 (AFI254Q) /CSM/AF/AFI2-C/AFI254Q.Seq.d/                       1467   0.0
CFI634 (CFI634Q) /CSM/CF/CFI6-B/CFI634Q.Seq.d/                       1465   0.0
CFF134 (CFF134Q) /CSM/CF/CFF1-B/CFF134Q.Seq.d/                       1465   0.0
SFJ685 (SFJ685Q) /CSM/SF/SFJ6-D/SFJ685Q.Seq.d/                       1451   0.0
CFC537 (CFC537Q) /CSM/CF/CFC5-B/CFC537Q.Seq.d/                       1449   0.0
SFK640 (SFK640Q) /CSM/SF/SFK6-B/SFK640Q.Seq.d/                       1447   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF090443|AF090443.1 Dictyostelium discoideum catalase (cat) mRNA, complete cds.  1513   0.0    1
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1513   0.0    1
AJ400964|AJ400964.1 Proteus mirabilis ORF1 DNA, katA gene and menE gene, wild type.    76   1e-16  2
AJ400965|AJ400965.1 Proteus mirabilis ORF1 DNA, katA gene and menE gene, strain PR.    76   1e-16  2
AB064338|AB064338.1 Candida boidinii CTA1 gene for peroxisomal catalase, complete cds.    82   1e-16  2
AR375090|AR375090.1 Sequence 96 from patent US 6605709.            76   1e-16  2
CC133697|CC133697.1 NDL.83G19.SP6 Notre Dame Liverpool Aedes aegypti genomic clone NDL.83G19, DNA sequence.    46   7e-14  6
E65314|E65314.1 Novel catalase, gene of said catalase and composition containing  said  catalase, and process for preparing catalase using the genetic engineering  technology.    86   9e-13  1
CD645756|CD645756.1 SSPG103 SS pectin Sclerotinia sclerotiorum cDNA similar to Peroxisomal catalase, mRNA sequence.    68   1e-12  2
BX554380|BX554380.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse15b01_q1c.    74   3e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF090443_1(AF090443|pid:none) Dictyostelium discoideum catalase ...   518   e-146
CQ796333_1(CQ796333|pid:none) Sequence 109 from Patent WO2004027...   291   2e-77
AE017333_3935(AE017333|pid:none) Bacillus licheniformis DSM 13, ...   291   2e-77
CP000560_826(CP000560|pid:none) Bacillus amyloliquefaciens FZB42...   282   7e-75
CP000300_481(CP000300|pid:none) Methanococcoides burtonii DSM 62...   281   2e-74
(O93662) RecName: Full=Catalase;          EC=1.11.1.6;  &AJ00593...   278   2e-73
(P26901) RecName: Full=Vegetative catalase;          EC=1.11.1.6...   273   4e-72
AY378080_1(AY378080|pid:none) Tetrahymena thermophila putative c...   271   1e-71
(Q9PWF7) RecName: Full=Catalase;          EC=1.11.1.6;  &AB03187...   263   6e-69
AY242059_1(AY242059|pid:none) Melopsittacus undulatus catalase (...   259   8e-68

 psg: 0.79  gvh: 0.23  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFG841 is nuc

>AFG841Z.Seq
----------GAANCCATCGCCAAAGGTGGTGAACCAGCTTGGAAAGTTTCCATTCAATT
GATGGAATTTGAAGATGCCTTAAAGTATCGTTTCAATCCATTCGATGTAACAAAGATTTG
GTCACACAAAGACTATCCATTAATTCAAATTGGTCGTATGGTTTTGAATCGTAATCCAGA
GAATTACTTTGCTGAAGTTGAACAAGCTGCCTTCTCACCATCCCACATGGTTCCAGGTAT
TGAACCATCACCAGATAAGATGTTACAAGGTCGTCTCTTCTCTTATCCAGATACTCATCG
TCATCGTTTAGGTGTAAACTATCAACAAATCCCAGTCAATTGTCCATTCGCCGTAAAGGG
TGGTGTTAAAAACTACCAACGTGATGGTTTCATGGCTGTCAATGGTAATGGTGGTAAAGG
TCCAAACTATCAACCAAACTCTTTCGGTGGTCCAGAGCCACATCCAGAATTTGCTCAACA
TAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAACCATACAATCATCCAAATGACGACTT
TGTTCAACCAGGTGATTTATATAGACTCATGTCTGAAGATGCCAAATCTCGTTTCGTCTC
TAATTTAGTCGGTCACATGTCCGGTGTCACCATCAAAGAAATTCAAGTTCGTGCCGTTTC
AAACTTTTACAAGGCTGACAAAGATTTAGGTGCTCGTTTATGTAAGGGTCTTGGTATTGA
TGTTAATNATGTCATCAAATTCGCTGCTAGATCAAACTTGTAAATCAATATATTAAAAA