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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFG475
Library SF
(Link to library)
Clone ID SFG475
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16243-1
Original site URL
Representative seq. ID SFG475E
(Link to Original site)
Representative DNA sequence 
>SFG475 (SFG475Q) /CSM/SF/SFG4-D/SFG475Q.Seq.d/
AAGAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAAC
TTCTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAAT
TTGTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGT
TATTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTAT
GGGAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATT
AGCAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTT
ACAAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGC
AGGCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGT
TGCCCAAGAGGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAA
TGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTCACA
CAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCAC
CGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAA
AACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCAC
CGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAG
TACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGG
TGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACA
AGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCA
AGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCTC
TGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTTC
AAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTCTATGTGTA
sequence update 2001.11.24
Translated Amino Acid sequence 
RIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAICKAKRGAFKETAPDDLLAPV
IKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLAGLPKEVPCYTVNRQCSSGL
QAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWDGGFNEVAQEDPIISGCYNTMGQTSEN
VAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDEGIREK
TTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVAFAAVG
VEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNKVNPNGS
GIALGHPLGATGARQMATISRELHRRKGKYGVISMC
Translated Amino Acid sequence (All Frames) 
Frame A:
kn*nyfsslkhwtnfriksnfwis*irr*ycncctisysnl*s*krsiqrdstr*fists
y*tyyqsyqirskinwrccygssitkiitrcnrs*schiisrftkgstmlysqqtmfirf
tsncqlcsssqsrfl*hwfsrrcrinefksngmgwwfqrgcprgsnh*wllqhygsnl*k
crrafwchqkrpr*ilckftqegrcrpksr*i*r*nrsshrkdrgwqnhrrr*rrryp*e
nhrcrfgqiktsfqrgwynhrw*l*sne*wcrrlfsyekkyrrkigsqg*igfrsfcccr
crtihygywssrcnssrlktswshh*ryrrf*nq*sfcfssilql*gfgfgyeqs*skwl
wyrfrssircywcpsngyhfkritss*r*iwcnlyv


Frame B:
RIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAICKAKRGAFKETAPDDLLAPV
IKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLAGLPKEVPCYTVNRQCSSGL
QAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWDGGFNEVAQEDPIISGCYNTMGQTSEN
VAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDEGIREK
TTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVAFAAVG
VEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNKVNPNGS
GIALGHPLGATGARQMATISRELHRRKGKYGVISMC


Frame C:
elklflvt*aldqf*nqiqlldqlnqemil*llhhfvqqfvklkeehskrqhqmiy*hql
lnilsklpn*iqn*lemllweqyyqdhhkvqpklelphy*qvyqrkyhviqstdnvhqvy
kqlpivqqqskqvsmtlv*qav*nq*v*iqwhgmvvstrlpkriqslvvvttlwvkplkm
sqsvlvspektkmnsl*ihtrrqvppkkqvnltmksfqsp*rprmakpsssiktkvsvrk
pplqiwpn*nqlskrvvqpplvtlvk*vmvpplv*l*kevppkdwvsrlnwfs*llllsv
snhplwvlvqplqfqpp*nklvsplkistflksmkllllkhftvvrfwvwi*tkliqmal
vsl*vih*vllvpvkwlpfqenyivvkvnmv*slcv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFG475 (SFG475Q) /CSM/SF/SFG4-D/SFG475Q.Seq.d/                       2359   0.0
SFA524 (SFA524Q) /CSM/SF/SFA5-A/SFA524Q.Seq.d/                       2359   0.0
CFF662 (CFF662Q) /CSM/CF/CFF6-C/CFF662Q.Seq.d/                       2359   0.0
CFC529 (CFC529Q) /CSM/CF/CFC5-B/CFC529Q.Seq.d/                       2353   0.0
AFE219 (AFE219Q) /CSM/AF/AFE2-A/AFE219Q.Seq.d/                       2353   0.0
VFE239 (VFE239Q) /CSM/VF/VFE2-B/VFE239Q.Seq.d/                       2351   0.0
SFD792 (SFD792Q) /CSM/SF/SFD7-D/SFD792Q.Seq.d/                       2351   0.0
CFC445 (CFC445Q) /CSM/CF/CFC4-B/CFC445Q.Seq.d/                       2351   0.0
AFE192 (AFE192Q) /CSM/AF/AFE1-D/AFE192Q.Seq.d/                       2333   0.0
AFA747 (AFA747Q) /CSM/AF/AFA7-B/AFA747Q.Seq.d/                       2333   0.0
own update 2002.12. 1
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  2222   0.0    1
CA917334|CA917334.1 EST641481 GPOD Medicago truncatula cDNA clone GPOD-35H18, mRNA sequence.    40   2e-18  7
CA858588|CA858588.1 EST635843 GLSD Medicago truncatula cDNA clone pGLSD-38M16, mRNA sequence.    40   2e-14  6
CB005315|CB005315.1 VVC011E10_126660 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC011E10 5, mRNA sequence.    60   2e-13  5
AU089542|AU089542.1 Lotus japonicus flower bud cDNA clone: Ljfb-v09.    44   1e-12  5
CB008288|CB008288.1 VVC055H06_148036 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC055H06 5, mRNA sequence.    60   1e-11  4
CF213585|CF213585.1 CGF1000794_F07 Vitis vinifera cv. cabernet sauvignon Stem - CAST Vitis vinifera cDNA clone CAST0006_IF_F07 5', mRNA sequence.    60   5e-10  3
BM065622|BM065622.1 KS07005E12 KS07 Capsicum annuum cDNA, mRNA sequence.    68   1e-09  3
CB893643|CB893643.1 EST646435 HOGA Medicago truncatula cDNA clone HOGA-28F15, mRNA sequence.    40   4e-09  5
CD007738|CD007738.1 VVB078G11_334604 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB078G11 5, mRNA sequence.    60   2e-08  2
dna update 2003.12.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_75(AC116986|pid:none) Dictyostelium discoideum chromoso...   752   0.0
X75329_1(X75329|pid:none) M.indica (Manila) THMF5 mRNA for 3-ket...   420   e-116
AM474896_9(AM474896|pid:none) Vitis vinifera contig VV79X001698....   418   e-115
S57792(S57792)acetyl-CoA C-acyltransferase (EC 2.3.1.16) B precu...   418   e-115
T07989(T07989) acetyl-CoA C-acyltransferase (EC 2.3.1.16) precur...   412   e-114
(Q56WD9) RecName: Full=3-ketoacyl-CoA thiolase 2, peroxisomal;  ...   412   e-114
AB333797_1(AB333797|pid:none) Glycine max mRNA for peroxisomal 3...   412   e-113
BT070433_1(BT070433|pid:none) Picea sitchensis clone WS02729_F15...   410   e-113
AX025481_1(AX025481|pid:none) Sequence 7 from Patent WO0032789.       409   e-113
EU964831_1(EU964831|pid:none) Zea mays clone 281881 3-ketoacyl-C...   409   e-112
protein update 2009. 7. 3
PSORT 

 psg: 0.84  gvh: 0.40  alm: 0.28  top: 0.33  tms: 0.07  mit: 0.33  mip: 0.03
 nuc: 0.02  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: nuclear
	 20.0 %: cytoplasmic
	 20.0 %: endoplasmic reticulum
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for SFG475 is nuc
5' end seq. ID SFG475F
5' end seq. 
>SFG475F.Seq
AAGAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAAC
TTCTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAAT
TTGTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGT
TATTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTAT
GGGAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATT
AGCAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTT
ACAAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGC
AGGCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGT
TGCCCAAGAGGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAA
TGTCGCAGNNNNNNNNNN
Length of 5' end seq. 558
3' end seq. ID SFG475Z
3' end seq. 
>SFG475Z.Seq
----------AACGAGGTTGCCCAAGAGGATCCAATCATTAGTGGTTGTTACAACACTAT
GGGTCAAACCTCTGAAAATGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGA
ATTCTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGA
TGAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGA
CGAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAA
AGAGGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTG
TTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGT
AGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAAT
TCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAA
TGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAA
AGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCG
TCAAATGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTC
TATGTGTA
Length of 3' end seq. 718
Connected seq. ID SFG475P
Connected seq. 
>SFG475P.Seq
AAGAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAAC
TTCTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAAT
TTGTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGT
TATTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTAT
GGGAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATT
AGCAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTT
ACAAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGC
AGGCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGT
TGCCCAAGAGGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAA
TGTCGCAG----------AACGAGGTTGCCCAAGAGGATCCAATCATTAGTGGTTGTTAC
AACACTATGGGTCAAACCTCTGAAAATGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGAC
CAAGATGAATTCTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAA
TTTGACGATGAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTC
GATAAAGACGAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCA
GCTTTCAAAGAGGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCC
GCCGCTTGTTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTG
GTTTTCGTAGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCC
GTTGCAATTCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTT
GAAATCAATGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGAT
ATGAACAAAGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACT
GGTGCCCGTCAAATGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGT
GTAATCTCTATGTGTA
Length of connected seq. 1266
Full length Seq ID SFG475E
Full length Seq. 
>SFG475E.Seq
AAGAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAAC
TTCTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAAT
TTGTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGT
TATTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTAT
GGGAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATT
AGCAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTT
ACAAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGC
AGGCGGTGTAGAATCAATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGT
TGCCCAAGAGGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAA
TGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTCTGTAAATTCACA
CAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCAC
CGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAA
AACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACCAC
CGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAG
TACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGG
TGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACA
AGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCA
AGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCTC
TGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTTC
AAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTCTATGTGTA
Length of full length seq. 1190