>SFI274 (SFI274Q) /CSM/SF/SFI2-D/SFI274Q.Seq.d/
XXXXXXXXXXTANGAACGTTTCTTAGGCCCAGAATTATTCTTCAATCCAGAGATTGCCTC
CTCNGNNTACCTCACCCCATTACCNAAGGTTGTCGNCGATACCATTCAATCCTGTCCAAT
CGANTGTNGTNGTGGTCTCNACAAGNATATCGTCNTATATGGTGGTTCAACCATGTTTAN
AGNTTTTGGTAAACGTCTTCAACGTGATGTCNAACGTTCTGTCGATTACCGTATCNAACG
TTCNNAAGAATTAGNTGGTGGTAAAATTAAAGCTGTCCCATTGGCCGTCAACGTTATCTC
TCACAATATGCAACGGTATGCCGTATGGTTTGGTGGTTCTATGTTGGCTTCAACTC

---ERFLGPELFFNPEIASSXYLTPLPKVVXDTIQSCPIXCXXGLXKXIVXYGGSTMFXX
FGKRLQRDVXRSVDYRIXRSXELXGGKIKAVPLAVNVISHNMQRYAVWFGGSMLAST

Frame A:
---xnvs*aqnyssiqrlppxxtsphyxrlsxipfnpvqsxvvvvstxissymvvqpclx
xlvnvfnvmsnvlsitvsnvxkn*xvvklklshwpstlslticngmpyglvvlcwlql


Frame B:
---xtflrpriilqsrdcllxxphpitxgcrryhsilsnrxxxwsxqxyrxiwwfnhvxx
fw*tsst*cxtfcrlpyxtfxrixww*n*scpigrqrylsqyatvcrmvwwfyvgfn


Frame C:
---ERFLGPELFFNPEIASSXYLTPLPKVVXDTIQSCPIXCXXGLXKXIVXYGGSTMFXX
FGKRLQRDVXRSVDYRIXRSXELXGGKIKAVPLAVNVISHNMQRYAVWFGGSMLAST

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFI274 (SFI274Q) /CSM/SF/SFI2-D/SFI274Q.Seq.d/                        573   e-163
AFN526 (AFN526Q) /CSM/AF/AFN5-B/AFN526Q.Seq.d/                        553   e-157
VSF457 (VSF457Q) /CSM/VS/VSF4-C/VSF457Q.Seq.d/                        549   e-156
VFD509 (VFD509Q) /CSM/VF/VFD5-A/VFD509Q.Seq.d/                        549   e-156
SFB660 (SFB660Q) /CSM/SF/SFB6-C/SFB660Q.Seq.d/                        549   e-156
AFJ410 (AFJ410Q) /CSM/AF/AFJ4-A/AFJ410Q.Seq.d/                        549   e-156
AFI845 (AFI845Q) /CSM/AF/AFI8-B/AFI845Q.Seq.d/                        549   e-156
AFI538 (AFI538Q) /CSM/AF/AFI5-B/AFI538Q.Seq.d/                        549   e-156
AFH594 (AFH594Q) /CSM/AF/AFH5-D/AFH594Q.Seq.d/                        549   e-156
AFH468 (AFH468Q) /CSM/AF/AFH4-C/AFH468Q.Seq.d/                        549   e-156

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Z46418|Z46418.1 Dictyostelium discoideum mRNA for actin-like protein.   549   e-153  1
CD462694|CD462694.1 ETH1_39_B12.b1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA clone ETH1_39_B12_A002 3', mRNA sequence.    44   2e-05  3
CB191690|CB191690.1 py35e07.y1 Haemonchus contortus whole worm pAMP1 v1 Haemonchus contortus cDNA 5' similar to WP:CE25554 Y71F9AL.16 actin ;, mRNA sequence.    54   0.001  1
CD566012|CD566012.1 tab89e01.x1 Hydra EST -III Hydra magnipapillata cDNA 3' similar to SW:ARP3_DROME P32392 ACTIN-LIKE PROTEIN 3 ;, mRNA sequence.    54   0.001  1
CD566031|CD566031.1 tab89g01.x1 Hydra EST -III Hydra magnipapillata cDNA 3' similar to SW:ARP3_DROME P32392 ACTIN-LIKE PROTEIN 3 ;, mRNA sequence.    54   0.001  1
AW682795|AW682795.1 SWYD25CAU13B02SK Brugia malayi young adult day 25 cDNA (SAW99MLW-BmYD25) Brugia malayi cDNA clone SWYD25CAU13B02 5', mRNA sequence.    46   0.022  2
AF098684|AF098684.1 Ceratitis rosa muscle specific actin gene, partial sequence.    46   0.32   1
AF098683|AF098683.1 Ceratitis capitata clone 012b muscle specific actin gene, partial sequence.    46   0.32   1
AF098682|AF098682.1 Ceratitis capitata clone 012a muscle specific actin gene, partial sequence.    46   0.32   1
AF098681|AF098681.1 Ceratitis capitata clone 36 muscle specific actin gene, partial sequence.    46   0.32   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY577012_1(AY577012|pid:none) Danio rerio clone RZ152A2C04 ARP3 ...   141   6e-33
BC064225_1(BC064225|pid:none) Xenopus tropicalis ARP3 actin-rela...   141   6e-33
BC096845_1(BC096845|pid:none) Danio rerio ARP3 actin-related pro...   141   8e-33
AB292042_1(AB292042|pid:none) Rattus norvegicus Arp3 mRNA for ac...   140   1e-32
AB292043_1(AB292043|pid:none) Rattus norvegicus Arp3 mRNA for ac...   140   1e-32
(Q90WD0) RecName: Full=Actin-related protein 3; AltName: Full=Ac...   140   1e-32
AK300030_1(AK300030|pid:none) Homo sapiens cDNA FLJ51082 complet...   140   1e-32
(Q4V7C7) RecName: Full=Actin-related protein 3; AltName: Full=Ac...   140   1e-32
AK302152_1(AK302152|pid:none) Homo sapiens cDNA FLJ52434 complet...   140   1e-32
DQ985463_1(DQ985463|pid:none) Suberites domuncula actin related ...   139   4e-32

 psg: 0.75  gvh: 0.43  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SFI274 is cyt

>SFI274Z.Seq
NNNNNNNNNNTANGAACGTTTCTTAGGCCCAGAATTATTCTTCAATCCAGAGATTGCCTC
CTCNGNNTACCTCACCCCATTACCNAAGGTTGTCGNCGATACCATTCAATCCTGTCCAAT
CGANTGTNGTNGTGGTCTCNACAAGNATATCGTCNTATATGGTGGTTCAACCATGTTTAN
AGNTTTTGGTAAACGTCTTCAACGTGATGTCNAACGTTCTGTCGATTACCGTATCNAACG
TTCNNAAGAATTAGNTGGTGGTAAAATTAAAGCTGTCCCATTGGCCGTCAACGTTATCTC
TCACAATATGCAACGGTATGCCGTATGGTTTGGTGGTTCTATGTTGGCTTCAACTC