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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFJ485
Library SF
(Link to library)
Clone ID SFJ485
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16560-1
Original site URL
Representative seq. ID SFJ485P
(Link to Original site)
Representative DNA sequence 
>SFJ485 (SFJ485Q) /CSM/SF/SFJ4-D/SFJ485Q.Seq.d/
AAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAAAATTTAAAAANNCNCCTTTTTNGAAAAAAAAAA
XXXXXXXXXXTAAAACCAAATNTTCAANAAAANGAAGGTNTTCCCCCAGGATCAACCACG
TTTTCATTTTCGTTGGTAAACCAATTAAAANATGNTCGNACTCTCTCTGATTACCAATAT
CCCAAAAAGAATCAACTCTCCACTTGGTCNTCAGATTAAGAGGTGGTATGCAAATCTTTG
TCAAAACATTAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATATTGAAA
ATGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACAAAGATTAATTT
TCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAAT
CCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCCTCA
CTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAGCCA
AAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGTAAAC
AATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATT
TAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAACCA
TTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAAGACA
AAGAAGGTATTCC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
KFIFIIQILNSYFIFLYIVTFHKKKKKKKNLKXXLFXKKK---

---KPNXQXXEGXPPGSTTFSFSLVNQLKXXRTLSDYQYPKKNQLSTWSSD*evvcksls
kh*lvrpsllklraqiilkmlkqrfktkkvshqtnkd*fslvnn*kmvvlslittfkrnp
lsi*ssd*evvckflskpslvrllhsklkvlitlrmlkpksktkkvfhqinnvsfspvnn
wrmvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsklkvvttlrm*kqkfktk
kvf
Translated Amino Acid sequence (All Frames) 
Frame A:
KFIFIIQILNSYFIFLYIVTFHKKKKKKKNLKXXLFXKKK---

---*nqxfxkxkvfpqdqprfhfrw*tn*xmxxlslitnipkrinsplgxqikrwyanlc
qninw*dhhs*s*glr*y*kc*nkdsrqrryptrptkinfrw*tirrwsysl*lqhskgi
hspfspqikrwyanfcqnphw*dyytrs*rf**h*ec*sqnprqrrystrsttshfrr*t
iggwsysl*lqhskgihspfssqikrwyanfcknshw*nhytrs*r**qh*ecknknsrq
rrys

Frame B:
nsfllyky*ihilffyil*hfikkkkkkki*kxxfxekk---

---ktkxsxkxrxsprinhvfifvgkpikxxsxsl*lpisqkestlhlvxrlrggmqifv
ktltgktitlevegsdnienvktkiqdkegippdqqrlifagkqledgrtlsdyniqkes
tlhlvlrlrggmqifvktltgktitlevegsdnienvkakiqdkegippdqqrlifagkq
ledgrtlsdyniqkestlhlvlrlrggmqifvktltgktitlevegsdnienvktkiqdk
egi

Frame C:
ihfyytnikfifyffiycnis*kkkkkkkfkxxpfxkkk---

---KPNXQXXEGXPPGSTTFSFSLVNQLKXXRTLSDYQYPKKNQLSTWSSD*evvcksls
kh*lvrpsllklraqiilkmlkqrfktkkvshqtnkd*fslvnn*kmvvlslittfkrnp
lsi*ssd*evvckflskpslvrllhsklkvlitlrmlkpksktkkvfhqinnvsfspvnn
wrmvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsklkvvttlrm*kqkfktk
kvf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFJ485 (SFJ485Q) /CSM/SF/SFJ4-D/SFJ485Q.Seq.d/                       1386   0.0
SFC110 (SFC110Q) /CSM/SF/SFC1-A/SFC110Q.Seq.d/                       1263   0.0
CFC401 (CFC401Q) /CSM/CF/CFC4-A/CFC401Q.Seq.d/                       1261   0.0
CHB343 (CHB343Q) /CSM/CH/CHB3-B/CHB343Q.Seq.d/                       1255   0.0
AFC682 (AFC682Q) /CSM/AF/AFC6-D/AFC682Q.Seq.d/                       1253   0.0
CFJ579 (CFJ579Q) /CSM/CF/CFJ5-D/CFJ579Q.Seq.d/                       1251   0.0
CFB409 (CFB409Q) /CSM/CF/CFB4-A/CFB409Q.Seq.d/                       1251   0.0
AFN862 (AFN862Q) /CSM/AF/AFN8-C/AFN862Q.Seq.d/                       1251   0.0
VFJ892 (VFJ892Q) /CSM/VF/VFJ8-D/VFJ892Q.Seq.d/                       1249   0.0
CHC365 (CHC365Q) /CSM/CH/CHC3-C/CHC365Q.Seq.d/                       1249   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M19491|M19491.1 Slime mold (D.discoideum) ubiquitin p229C/pLK229 gene, complete cds.  1193   0.0    3
M23748|M23748.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB19.  1178   0.0    3
M19666|M19666.1 Slime mold (D.discoideum) ubiquitin gene, 3' end, clone p229C.  1178   0.0    3
M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   690   0.0    3
M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   678   0.0    2
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   662   0.0    2
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   543   e-156  3
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   543   e-156  3
BJ541175|BJ541175.1 Oryzias latipes cDNA clone:MF01SSB034A18, 3' end single read.   176   2e-93  4
Z54197|Z54197.1 C.albicans UBI4 gene encoding polyubiquitin.      170   3e-93  4
dna update 2004. 1. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   394   e-109
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   394   e-109
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   394   e-109
M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   389   e-109
AB021974_1(AB021974|pid:none) Bombyx mori UBI3 mRNA for polyubiq...   375   e-104
T16144(T16144) ubiquitin - Caenorhabditis elegans  &U23172_1(U23...   375   e-104
S32020(S32020) polyubiquitin 6 - Geodia cydonium  &X70917_1(X709...   375   e-104
Y12081_1(Y12081|pid:none) Suberites domuncula mRNA for polyubiqu...   375   e-104
AY118702_1(AY118702|pid:none) Drosophila melanogaster AT20865 fu...   373   e-104
AB003731_1(AB003731|pid:none) Cricetulus griseus gene for polyub...   373   e-104
protein update 2009. 5.27
PSORT 

 psg: 0.96  gvh: 0.37  alm: 0.26  top: 0.97  tms: 0.07  mit: 0.29  mip: 0.00
 nuc: 0.55  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: Golgi
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for SFJ485 is nuc
5' end seq. ID SFJ485F
5' end seq. 
>SFJ485F.Seq
AAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAAAATTTAAAAANNCNCCTTTTTNGAAAAAAAAAA
----------
Length of 5' end seq. 120
3' end seq. ID SFJ485Z
3' end seq. 
>SFJ485Z.Seq
----------TAAAACCAAATNTTCAANAAAANGAAGGTNTTCCCCCAGGATCAACCACG
TTTTCATTTTCGTTGGTAAACCAATTAAAANATGNTCGNACTCTCTCTGATTACCAATAT
CCCAAAAAGAATCAACTCTCCACTTGGTCNTCAGATTAAGAGGTGGTATGCAAATCTTTG
TCAAAACATTAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATATTGAAA
ATGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACAAAGATTAATTT
TCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAAT
CCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCCTCA
CTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAGCCA
AAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGTAAAC
AATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATT
TAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAACCA
TTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAAGACA
AAGAAGGTATTCC
Length of 3' end seq. 723
Connected seq. ID SFJ485P
Connected seq. 
>SFJ485P.Seq
AAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTTATATATTGTAACA
TTTCATAAAAAAAAAAAAAAAAAAAAAAATTTAAAAANNCNCCTTTTTNGAAAAAAAAAA
----------TAAAACCAAATNTTCAANAAAANGAAGGTNTTCCCCCAGGATCAACCACG
TTTTCATTTTCGTTGGTAAACCAATTAAAANATGNTCGNACTCTCTCTGATTACCAATAT
CCCAAAAAGAATCAACTCTCCACTTGGTCNTCAGATTAAGAGGTGGTATGCAAATCTTTG
TCAAAACATTAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATATTGAAA
ATGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACAAAGATTAATTT
TCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAAT
CCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCCTCA
CTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAGCCA
AAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGTAAAC
AATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATT
TAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAACCA
TTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAAGACA
AAGAAGGTATTCC
Length of connected seq. 843
Full length Seq ID -
Full length Seq. -
Length of full length seq. -