>SHD327 (SHD327Q) /CSM/SH/SHD3-B/SHD327Q.Seq.d/
XXXXXXXXXXTCNCAAATGTTAAATTGGATAACTTGTATACAGTNTGCTCAATCCATTTC
AATGGAAGCTTATTTGAAATATAAAACTGGTGGTGGTGGTAGTGGTGGTAGTGGTGGTGG
TGGTCATAGTAGTCATTCAAGAACAAATAGCAATACTAGTAGTAGCAGTGTAAATAGTAG
TAATCGTTTAAGTATGAGATTGAAAGAAGATGATGTTGAAGATGGTGTTCAAAAAATGTT
GGATGAAAATAAAGAATCACTAAACAAACTATTGGAACAAGAGGATAATAAATATTGCTC
AGATTGTGGTTGTCCATCACCATTGTGGGCATCAATTAATTTGGGTGTTTTCATTTGTAT
CAATTGTAGTGGTGT

---QMLNWITCIQXAQSISMEAYLKYKTGGGGSGGSGGGGHSSHSRTNSNTSSSSVNSSN
RLSMRLKEDDVEDGVQKMLDENKESLNKLLEQEDNKYCSDCGCPSPLWASINLGVFICIN
CSG

Frame A:
---xkc*ig*lvysxlnpfqwkli*niklvvvvvvvvvvvvivviqeqiailvvav*ivv
iv*v*d*kkmmlkmvfkkcwmkiknh*tnywnkriiniaqivvvhhhcghqliwvfsfvs
ivvv


Frame B:
---xnvkldnlytvcsihfngslfei*nwwww*ww*wwwws**sfknk*qy***qck***
sfkyeierr*c*rwcsknvg*k*ritkqtigtrg**illrlwlsitivgin*fgcfhlyq
l*wc


Frame C:
---QMLNWITCIQXAQSISMEAYLKYKTGGGGSGGSGGGGHSSHSRTNSNTSSSSVNSSN
RLSMRLKEDDVEDGVQKMLDENKESLNKLLEQEDNKYCSDCGCPSPLWASINLGVFICIN
CSG

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHD327 (SHD327Q) /CSM/SH/SHD3-B/SHD327Q.Seq.d/                        498   e-140
CHL519 (CHL519Q) /CSM/CH/CHL5-A/CHL519Q.Seq.d/                        498   e-140
CHA212 (CHA212Q) /CSM/CH/CHA2-A/CHA212Q.Seq.d/                        498   e-140
AFO311 (AFO311Q) /CSM/AF/AFO3-A/AFO311Q.Seq.d/                        498   e-140
CHE716 (CHE716Q) /CSM/CH/CHE7-A/CHE716Q.Seq.d/                        484   e-136
AHN342 (AHN342Q) /CSM/AH/AHN3-B/AHN342Q.Seq.d/                        478   e-134
AHA633 (AHA633Q) /CSM/AH/AHA6-B/AHA633Q.Seq.d/                        478   e-134
AFM139 (AFM139Q) /CSM/AF/AFM1-B/AFM139Q.Seq.d/                        474   e-133
AHO517 (AHO517Q) /CSM/AH/AHO5-A/AHO517Q.Seq.d/                        466   e-130
AHF654 (AHF654Q) /CSM/AH/AHF6-C/AHF654Q.Seq.d/                        466   e-130

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.   466   e-163  2
AY220914|AY220914.1 Dictyostelium discoideum ComB (comB) gene, complete cds.    36   0.34   2
BM168885|BM168885.1 EST571408 PyBS Plasmodium yoelii yoelii cDNA clone PYCPI56 5' end, mRNA sequence.    46   0.53   1
AC097155|AC097155.5 Rattus norvegicus clone CH230-140A7, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    46   0.53   1
AQ873059|AQ873059.1 V58E6 mTn-3xHA/lacZ Insertion Library, strain AB972 Saccharomyces cerevisiae genomic 5', genomic survey sequence.    46   0.53   1
CR380956|CR380956.1 Candida glabrata strain CBS138 chromosome J complete sequence.    40   0.64   6
AC078840|AC078840.6 Oryza sativa chromosome 10 BAC OSJNBb0073N24 genomic sequence, complete sequence.    34   1.1    2
AC172790|AC172790.2 Bos taurus clone CH240-263B13, WORKING DRAFT SEQUENCE, 6 unordered pieces.    44   2.1    1
AC171138|AC171138.1 Helobdella robusta clone CH306-1A9, WORKING DRAFT SEQUENCE, 5 unordered pieces.    44   2.1    1
AQ270585|AQ270585.1 HS_2047_B1_A03_MR CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=2047 Col=5 Row=B, genomic survey sequence.    44   2.1    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC013483_28(AC013483|pid:none) Arabidopsis thaliana chromosome I...    67   1e-10
(Q8L7A4) RecName: Full=Probable ADP-ribosylation factor GTPase-a...    67   1e-10
(Q8IYB5) RecName: Full=Stromal membrane-associated protein 1;  &...    66   4e-10
EF116598_1(EF116598|pid:none) Canis lupus familiaris SMAP1 prote...    66   4e-10
BC126835_1(BC126835|pid:none) Bos taurus similar to stromal memb...    66   4e-10
AK023221_1(AK023221|pid:none) Homo sapiens cDNA FLJ13159 fis, cl...    66   4e-10
BC124755_1(BC124755|pid:none) Danio rerio stromal membrane-assoc...    65   5e-10
BC071454_1(BC071454|pid:none) Danio rerio stromal membrane-assoc...    65   5e-10
BX324154_1(BX324154|pid:none) Zebrafish DNA sequence from clone ...    65   5e-10
BC073437_1(BC073437|pid:none) Xenopus laevis hypothetical protei...    64   1e-09

 psg: 0.89  gvh: 0.54  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.11
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 24.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for SHD327 is nuc

>SHD327Z.Seq
NNNNNNNNNNTCNCAAATGTTAAATTGGATAACTTGTATACAGTNTGCTCAATCCATTTC
AATGGAAGCTTATTTGAAATATAAAACTGGTGGTGGTGGTAGTGGTGGTAGTGGTGGTGG
TGGTCATAGTAGTCATTCAAGAACAAATAGCAATACTAGTAGTAGCAGTGTAAATAGTAG
TAATCGTTTAAGTATGAGATTGAAAGAAGATGATGTTGAAGATGGTGTTCAAAAAATGTT
GGATGAAAATAAAGAATCACTAAACAAACTATTGGAACAAGAGGATAATAAATATTGCTC
AGATTGTGGTTGTCCATCACCATTGTGGGCATCAATTAATTTGGGTGTTTTCATTTGTAT
CAATTGTAGTGGTGT