>SHF264 (SHF264Q) /CSM/SH/SHF2-C/SHF264Q.Seq.d/
ACATATCGATAAAATATCTTGTGATTTGTATTGACTCGTGTTCAACAATAAACAGAAGAA
AGATGTCAACATTAAAGAATAAAAAGCTAATACAAATAGAAGGGGAGATTGAAAAAGCAA
GACAAGATAAAAATTGGAATGCTTTAACCGATTTAATTAGAAAATATAATAAATTAAATA
CAGATAATACAGTACCACTTGAATTATTAATAAATAGTGAGAAATATTTAACAGAATTTG
ATTATAACAATGCATTGGCAACATTGAATAAAGCATATAATATAGCACCAAATAATTCAG
AAGTACTTTCATATTTAGGTATTGTTGAATATGAAAGAAATGATATAGTTAAAGCATTAT
CATATCTTAATCGTTTAAATAATCCATATGGTAATAATGAAAATGGTAATGGTAATGGTA
ATGGTAGTGGTAATGATATGATTTTAAATAATCGTAAAGTTGGTTTATTAATTCAAGCAT
TTACAATCAAAGGTAAATGTTATGAAAATCAAGGTAATCAACAAGATGCAATCACAAGTT
ATCAAAATGTTTATCAAATCACTTTTAAATATTTTAAAAATTTTAAAACAATTGATAAAC
AAACTAAATTATATATTGAAGATTCTTTAATTAXXXXXXXXXXGAAGATATTGATGATTC
AGTTAGTTTATCAAGAAATAGACAATCTGGACCAAGTAGTGTTGTATCATTTGATTTAAG
ATCAGGTACAACAGCTGGTGATAGTAGATCACATAGATCTTATTCAATTAATAATGCAGA
GATGGATTCAGCAATGAGAGACTTTGAAGATAGTCTTACAAGTCCAAAAGATTTATTAAG
AACTACAACAAATAGTAGTAGTAGTGGAGTAAATGGCGGTAATAGTGGTGGTGGTGGCAG
TAGTGGTGGTCATTGTTTATCAAGTAAAGAAGTTCATAGAAATATTGCATTATGGTTATC
ATTAGCAGAAGCATTCACTCAACAACGTATGTTTAAAGATGCGGCTCAATGTTTAGCACA
AGCTGAATCAATCGATAATGATTCAGCCGATGTTTATTATCATCAAGGTTATCTATTGGA
AACTCAAGATTTAATGGGAAAAGCAATTGCAAACTATCATAAAGCTTTAACTATTGATTC
AACTCATACAAATAGTTCAATTCGTTTAGGCTCTTTATTACTTTAGAGAGAATGATTTAT
TATTAGCTGAAAATAATCTCACTACAATCTTACGTTCTAGTGATCCAACTTCACATCAAG
CTTGGTTTCAATTAGGTTTAGTTTTAAAAGCTAAAGGTGAAATCGAACGTTCCTCTGATT
GTTTTAAAA

ISIKYLVICIDSCSTINRRKMSTLKNKKLIQIEGEIEKARQDKNWNALTDLIRKYNKLNT
DNTVPLELLINSEKYLTEFDYNNALATLNKAYNIAPNNSEVLSYLGIVEYERNDIVKALS
YLNRLNNPYGNNENGNGNGNGSGNDMILNNRKVGLLIQAFTIKGKCYENQGNQQDAITSY
QNVYQITFKYFKNFKTIDKQTKLYIEDSLI---

---EDIDDSVSLSRNRQSGPSSVVSFDLRSGTTAGDSRSHRSYSINNAEMDSAMRDFEDS
LTSPKDLLRTTTNSSSSGVNGGNSGGGGSSGGHCLSSKEVHRNIALWLSLAEAFTQQRMF
KDAAQCLAQAESIDNDSADVYYHQGYLLETQDLMGKAIANYHKALTIDSTHTNSSIRLGS
LLL*re*fiis*k*shynltf**snftsslvsirfsfks*r*nrtfl*lf*

Frame A:
tyr*nil*fvltrvqq*teercqh*riks*yk*kgrlkkqdkikigml*pi*leniin*i
qiiqyhlny**ivrni*qnliitmhwqh*ikhii*hqiiqkyfhi*vllnmkemi*lkhy
hiliv*iihmvimkmvmvmvmvvvmi*f*iivklvy*fkhlqskvnvmkikvinkmqsqv
ikmfiksllnilkilkqlinklnyilkil*l---

---EDIDDSVSLSRNRQSGPSSVVSFDLRSGTTAGDSRSHRSYSINNAEMDSAMRDFEDS
LTSPKDLLRTTTNSSSSGVNGGNSGGGGSSGGHCLSSKEVHRNIALWLSLAEAFTQQRMF
KDAAQCLAQAESIDNDSADVYYHQGYLLETQDLMGKAIANYHKALTIDSTHTNSSIRLGS
LLL*re*fiis*k*shynltf**snftsslvsirfsfks*r*nrtfl*lf*

Frame B:
hidkiscdly*lvfnnkqkkdvnike*kantnrrgd*ksktr*klecfnrfn*ki**iky
r*ystt*iink**eifnri*l*qcignie*si*ystk*frstfifryc*i*kk*ys*sii
is*sfk*siw***kw*w*w*w*w**ydfk*s*swfinssiynqr*ml*ksr*strcnhkl
skclsnhf*if*kf*nn**tn*iiy*rffn---

---kilmiqlvyqeidnldqvvlyhli*dqvqqlvivdhidliqlimqrwiqq*etlkiv
lqvqkiy*elqqivvvve*mavivvvvavvvvivyqvkkfieilhygyh*qkhslnnvcl
kmrlnv*hklnqsimiqpmfiiikviywklki*wekqlqtiikl*lliqliqivqfv*al
yyfrendlllaennlttilrssdptshqawfqlglvlkakgeierssdcfk

Frame C:
ISIKYLVICIDSCSTINRRKMSTLKNKKLIQIEGEIEKARQDKNWNALTDLIRKYNKLNT
DNTVPLELLINSEKYLTEFDYNNALATLNKAYNIAPNNSEVLSYLGIVEYERNDIVKALS
YLNRLNNPYGNNENGNGNGNGSGNDMILNNRKVGLLIQAFTIKGKCYENQGNQQDAITSY
QNVYQITFKYFKNFKTIDKQTKLYIEDSLI---

---ry**fs*fikk*tiwtk*ccii*fkirynsw***it*ilfn**crdgfsnerl*r*s
ykskrfiknynk****wskwr**wwwwq*wwslfik*rss*kycimviisrsihsttyv*
rcgsmfsts*inr**fsrcllssrlsignsrfngksnckls*sfny*fnsyk*fnsfrlf
itlermiyy*lkiislqsyvlviqlhiklgfn*v*f*klkvksnvplivlk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF264 (SHF264Q) /CSM/SH/SHF2-C/SHF264Q.Seq.d/                       1713   0.0
CHR104 (CHR104Q) /CSM/CH/CHR1-A/CHR104Q.Seq.d/                       1336   0.0
CHF888 (CHF888Q) /CSM/CH/CHF8-D/CHF888Q.Seq.d/                       1336   0.0
AHQ419 (AHQ419Q) /CSM/AH/AHQ4-A/AHQ419Q.Seq.d/                       1336   0.0
AHL167 (AHL167Q) /CSM/AH/AHL1-C/AHL167Q.Seq.d/                       1336   0.0
AHO863 (AHO863Q) /CSM/AH/AHO8-C/AHO863Q.Seq.d/                       1328   0.0
SHL618 (SHL618Q) /CSM/SH/SHL6-A/SHL618Q.Seq.d/                       1322   0.0
VHN261 (VHN261Q) /CSM/VH/VHN2-C/VHN261Q.Seq.d/                       1320   0.0
VHF147 (VHF147Q) /CSM/VH/VHF1-B/VHF147Q.Seq.d/                       1289   0.0
CHQ122 (CHQ122Q) /CSM/CH/CHQ1-A/CHQ122Q.Seq.d/                       1253   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AP006309|AP006309.1 Homo sapiens genomic DNA, chromosome 3 clone:RP11-39I4, complete sequence.    48   1e-04  2
AC146997|AC146997.2 Arbacia punctulata clone BAP-144G2, WORKING DRAFT SEQUENCE, 13 unordered pieces.    44   1e-04  2
AC181709|AC181709.1 Strongylocentrotus purpuratus clone R3-1039L24, WORKING DRAFT SEQUENCE, 11 unordered pieces.    48   4e-04  2
AC117883|AC117883.5 Rattus norvegicus clone CH230-101A3, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    30   0.001  3
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    38   0.001  17
AC110633|AC110633.4 Rattus norvegicus clone CH230-262J17, *** SEQUENCING IN PROGRESS ***, 104 unordered pieces.    40   0.001  2
AC110516|AC110516.6 Mus musculus clone RP23-203M17, WORKING DRAFT SEQUENCE, 10 ordered pieces.    44   0.002  2
AC026840|AC026840.4 Homo sapiens chromosome 15 clone RP11-526M10 map 15, WORKING DRAFT SEQUENCE, 11 unordered pieces.    46   0.002  2
BX000350|BX000350.4 Mouse DNA sequence *** SEQUENCING IN PROGRESS *** from clone  RP23-156M10.    44   0.002  2
BX247872|BX247872.12 Zebrafish DNA sequence from clone CH211-223M9.    44   0.002  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT060530_1(BT060530|pid:none) Zea mays full-length cDNA clone ZM...    47   8e-09
BT037015_1(BT037015|pid:none) Zea mays full-length cDNA clone ZM...    47   9e-09
AP008217_1498(AP008217|pid:none) Oryza sativa (japonica cultivar...    46   9e-07
BC098811_1(BC098811|pid:none) Rattus norvegicus tetratricopeptid...    44   6e-06
AK165244_1(AK165244|pid:none) Mus musculus 6 days neonate spleen...    44   9e-06
AK073968_1(AK073968|pid:none) Oryza sativa Japonica Group cDNA c...    38   2e-05
AF250191_1(AF250191|pid:none) Zea mays calmodulin-binding protei...    38   9e-05
CP000554_2385(CP000554|pid:none) Prochlorococcus marinus str. MI...    40   0.001
AP008209_648(AP008209|pid:none) Oryza sativa (japonica cultivar-...    46   0.003
CP000477_86(CP000477|pid:none) Methanosaeta thermophila PT, comp...    37   0.006

 psg: 0.53  gvh: 0.48  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: mitochondrial
	 28.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SHF264 is cyt

>SHF264F.Seq
ACATATCGATAAAATATCTTGTGATTTGTATTGACTCGTGTTCAACAATAAACAGAAGAA
AGATGTCAACATTAAAGAATAAAAAGCTAATACAAATAGAAGGGGAGATTGAAAAAGCAA
GACAAGATAAAAATTGGAATGCTTTAACCGATTTAATTAGAAAATATAATAAATTAAATA
CAGATAATACAGTACCACTTGAATTATTAATAAATAGTGAGAAATATTTAACAGAATTTG
ATTATAACAATGCATTGGCAACATTGAATAAAGCATATAATATAGCACCAAATAATTCAG
AAGTACTTTCATATTTAGGTATTGTTGAATATGAAAGAAATGATATAGTTAAAGCATTAT
CATATCTTAATCGTTTAAATAATCCATATGGTAATAATGAAAATGGTAATGGTAATGGTA
ATGGTAGTGGTAATGATATGATTTTAAATAATCGTAAAGTTGGTTTATTAATTCAAGCAT
TTACAATCAAAGGTAAATGTTATGAAAATCAAGGTAATCAACAAGATGCAATCACAAGTT
ATCAAAATGTTTATCAAATCACTTTTAAATATTTTAAAAATTTTAAAACAATTGATAAAC
AAACTAAATTATATATTGAAGATTCTTTAATTANNNNNNNNNN

>SHF264Z.Seq
NNNNNNNNNNGAAGATATTGATGATTCAGTTAGTTTATCAAGAAATAGACAATCTGGACC
AAGTAGTGTTGTATCATTTGATTTAAGATCAGGTACAACAGCTGGTGATAGTAGATCACA
TAGATCTTATTCAATTAATAATGCAGAGATGGATTCAGCAATGAGAGACTTTGAAGATAG
TCTTACAAGTCCAAAAGATTTATTAAGAACTACAACAAATAGTAGTAGTAGTGGAGTAAA
TGGCGGTAATAGTGGTGGTGGTGGCAGTAGTGGTGGTCATTGTTTATCAAGTAAAGAAGT
TCATAGAAATATTGCATTATGGTTATCATTAGCAGAAGCATTCACTCAACAACGTATGTT
TAAAGATGCGGCTCAATGTTTAGCACAAGCTGAATCAATCGATAATGATTCAGCCGATGT
TTATTATCATCAAGGTTATCTATTGGAAACTCAAGATTTAATGGGAAAAGCAATTGCAAA
CTATCATAAAGCTTTAACTATTGATTCAACTCATACAAATAGTTCAATTCGTTTAGGCTC
TTTATTACTTTAGAGAGAATGATTTATTATTAGCTGAAAATAATCTCACTACAATCTTAC
GTTCTAGTGATCCAACTTCACATCAAGCTTGGTTTCAATTAGGTTTAGTTTTAAAAGCTA
AAGGTGAAATCGAACGTTCCTCTGATTGTTTTAAAA

>SHF264P.Seq
ACATATCGATAAAATATCTTGTGATTTGTATTGACTCGTGTTCAACAATAAACAGAAGAA
AGATGTCAACATTAAAGAATAAAAAGCTAATACAAATAGAAGGGGAGATTGAAAAAGCAA
GACAAGATAAAAATTGGAATGCTTTAACCGATTTAATTAGAAAATATAATAAATTAAATA
CAGATAATACAGTACCACTTGAATTATTAATAAATAGTGAGAAATATTTAACAGAATTTG
ATTATAACAATGCATTGGCAACATTGAATAAAGCATATAATATAGCACCAAATAATTCAG
AAGTACTTTCATATTTAGGTATTGTTGAATATGAAAGAAATGATATAGTTAAAGCATTAT
CATATCTTAATCGTTTAAATAATCCATATGGTAATAATGAAAATGGTAATGGTAATGGTA
ATGGTAGTGGTAATGATATGATTTTAAATAATCGTAAAGTTGGTTTATTAATTCAAGCAT
TTACAATCAAAGGTAAATGTTATGAAAATCAAGGTAATCAACAAGATGCAATCACAAGTT
ATCAAAATGTTTATCAAATCACTTTTAAATATTTTAAAAATTTTAAAACAATTGATAAAC
AAACTAAATTATATATTGAAGATTCTTTAATTA----------GAAGATATTGATGATTC
AGTTAGTTTATCAAGAAATAGACAATCTGGACCAAGTAGTGTTGTATCATTTGATTTAAG
ATCAGGTACAACAGCTGGTGATAGTAGATCACATAGATCTTATTCAATTAATAATGCAGA
GATGGATTCAGCAATGAGAGACTTTGAAGATAGTCTTACAAGTCCAAAAGATTTATTAAG
AACTACAACAAATAGTAGTAGTAGTGGAGTAAATGGCGGTAATAGTGGTGGTGGTGGCAG
TAGTGGTGGTCATTGTTTATCAAGTAAAGAAGTTCATAGAAATATTGCATTATGGTTATC
ATTAGCAGAAGCATTCACTCAACAACGTATGTTTAAAGATGCGGCTCAATGTTTAGCACA
AGCTGAATCAATCGATAATGATTCAGCCGATGTTTATTATCATCAAGGTTATCTATTGGA
AACTCAAGATTTAATGGGAAAAGCAATTGCAAACTATCATAAAGCTTTAACTATTGATTC
AACTCATACAAATAGTTCAATTCGTTTAGGCTCTTTATTACTTTAGAGAGAATGATTTAT
TATTAGCTGAAAATAATCTCACTACAATCTTACGTTCTAGTGATCCAACTTCACATCAAG
CTTGGTTTCAATTAGGTTTAGTTTTAAAAGCTAAAGGTGAAATCGAACGTTCCTCTGATT
GTTTTAAAA