>AFJ576 (AFJ576Q) /CSM/AF/AFJ5-D/AFJ576Q.Seq.d/
ATGTTGAAGATAAAGAAGGTGAAATTAAGGCTTGTGTTGCAGAACCACATGATGGGGAAG
TAACAAAAGGAAATGGAGAAGAAGAAGAACCAAAAATTAAAGAAGATTTAAAAGTTCAAT
TAGAACCAAAGGTTGAAAAAATTGAAACACCAAAGAATACAAATATTTTAGTTTTAGGTA
GTACTGGTGTTGGTAAAAGTACATTTATTTCATCATTGGTAAACTATTTGAAATATGAAT
CATTGGAACAAGCTATGAATCTTGCCAGTTTGGATAATGTTGCCACCATTCCTAATAANG
TTCAAATTGTTGATTCGAAATAATGTATCACATAGTTTCGTTGTTGGTAAACTCAAAATA
CCTTTCACAAXXXXXXXXXX

VEDKEGEIKACVAEPHDGEVTKGNGEEEEPKIKEDLKVQLEPKVEKIETPKNTNILVLGS
TGVGKSTFISSLVNYLKYESLEQAMNLASLDNVATIPNXVQIVDSK*cit*frcw*tqnt
fh---

Frame A:
mlkikkvklrlvlqnhmmgk*qkemekkknqklkki*kfn*nqrlkklkhqriqif*f*v
vlvlvkvhlfhhw*ti*nmnhwnkl*ilpvwimlppflixfkllirnnvshsfvvgklki
pft---


Frame B:
c*r*rr*n*glccrtt*wgsnkrkwrrrrtkn*rrfkssirtkg*kn*ntkeykyfsfr*
ywcw*kyiyfiigklfei*iigtsyescqfg*cchhs**xsnc*feimyhivsllvnsky
ls---


Frame C:
VEDKEGEIKACVAEPHDGEVTKGNGEEEEPKIKEDLKVQLEPKVEKIETPKNTNILVLGS
TGVGKSTFISSLVNYLKYESLEQAMNLASLDNVATIPNXVQIVDSK*cit*frcw*tqnt
fh---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ576 (AFJ576Q) /CSM/AF/AFJ5-D/AFJ576Q.Seq.d/                        728   0.0
VHF237 (VHF237Q) /CSM/VH/VHF2-B/VHF237Q.Seq.d/                        696   0.0
VHC723 (VHC723Q) /CSM/VH/VHC7-A/VHC723Q.Seq.d/                        696   0.0
SFL483 (SFL483Q) /CSM/SF/SFL4-D/SFL483Q.Seq.d/                        696   0.0
SFH491 (SFH491Q) /CSM/SF/SFH4-D/SFH491Q.Seq.d/                        696   0.0
SFG571 (SFG571Q) /CSM/SF/SFG5-C/SFG571Q.Seq.d/                        696   0.0
SFF657 (SFF657Q) /CSM/SF/SFF6-C/SFF657Q.Seq.d/                        696   0.0
SFF558 (SFF558Q) /CSM/SF/SFF5-C/SFF558Q.Seq.d/                        696   0.0
CHP309 (CHP309Q) /CSM/CH/CHP3-A/CHP309Q.Seq.d/                        696   0.0
CHH828 (CHH828Q) /CSM/CH/CHH8-B/CHH828Q.Seq.d/                        696   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF474341|AF474341.1 Dictyostelium discoideum ABC transporter ABCC.9 (abcC9) gene, complete cds.    38  0.052  3
AP003361|AP003361.2 Staphylococcus aureus subsp. aureus Mu50 genomic DNA, complete sequence, section 4/9.    40  0.055  9
CG401790|CG401790.1 ZMMBBb0243G21.r ZMMBBb Zea mays subsp. mays genomic clone ZMMBBb0243G21 3', genomic survey sequence.    48  0.079  1
BD114463|BD114463.1 EST and encoded human protein.                36  0.12  2
BG612536|BG612536.1 602640335F1 NIH_MGC_61 Homo sapiens cDNA clone IMAGE:4771208 5', mRNA sequence.    36  0.16  2
BQ350669|BQ350669.1 RC3-HT0600-240400-023-d08 HT0600 Homo sapiens cDNA, mRNA sequence.    36  0.16  2
AV707713|AV707713.1 Homo sapiens cDNA clone:ADBDRH04, 5'end, expressed in human adrenal gland.    36  0.17  2
BD061025|BD061025.1 Secreted expressed sequence tags (sESTs).     36  0.17  2
BQ432883|BQ432883.1 AGENCOURT_7838667 NIH_MGC_82 Homo sapiens cDNA clone IMAGE:6101582 5', mRNA sequence.    36  0.19  2
CD642349|CD642349.1 AGENCOURT_14575541 NIA Human H1 Embryonic Stem Cell cDNA Library (Long) Homo sapiens cDNA clone IMAGE:30420833 5', mRNA sequence.    36  0.21  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB120174_1(AB120174|pid:none) Dictyostelium discoideum DD8-14 mR...   205   7e-54
AC114263_3(AC114263|pid:none) Dictyostelium discoideum chromosom...    52   8e-06
AM910995_3(AM910995|pid:none) Plasmodium knowlesi strain H chrom...    40   0.032
AE014188_15(AE014188|pid:none) Plasmodium falciparum 3D7 chromos...    39   0.071
AE014187_562(AE014187|pid:none) Plasmodium falciparum 3D7 chromo...    39   0.071
CP000619_85(CP000619|pid:none) Burkholderia vietnamiensis G4 pla...    39   0.071
EF531339_210(EF531339|pid:none) Candidatus Chloracidobacterium t...    38   0.092
AE008691_1361(AE008691|pid:none) Thermoanaerobacter tengcongensi...    38   0.092
CP001205_72(CP001205|pid:none) Borrelia burgdorferi ZS7, complet...    38   0.12
AM502241_24(AM502241|pid:none) Leishmania infantum chromosome 23.      38   0.12

 psg: 0.75  gvh: 0.17  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for AFJ576 is nuc

>AFJ576F.Seq
ATGTTGAAGATAAAGAAGGTGAAATTAAGGCTTGTGTTGCAGAACCACATGATGGGGAAG
TAACAAAAGGAAATGGAGAAGAAGAAGAACCAAAAATTAAAGAAGATTTAAAAGTTCAAT
TAGAACCAAAGGTTGAAAAAATTGAAACACCAAAGAATACAAATATTTTAGTTTTAGGTA
GTACTGGTGTTGGTAAAAGTACATTTATTTCATCATTGGTAAACTATTTGAAATATGAAT
CATTGGAACAAGCTATGAATCTTGCCAGTTTGGATAATGTTGCCACCATTCCTAATAANG
TTCAAATTGTTGATTCGAAATAATGTATCACATAGTTTCGTTGTTGGTAAACTCAAAATA
CCTTTCACAA----------