>AFJ789 (AFJ789Q) /CSM/AF/AFJ7-D/AFJ789Q.Seq.d/
TTAAAATTCAAATTATCAACAACATGGCAGAAAATAATAATAATAATAATAATAATAATA
ATAATAATGTTTCAACACCACCACATCAAAAACCACATTTAACAACTGGTTTAAGAACTT
CAAGTTCTGGTTTATTAATGGATAAAAGAAGACAAGATGAAGAAAAATTTTCAGCTGAAC
AAGTAGCAATGGGTAAAAAATCACCAACAAAATTATTCTCAGCATACAGCTTAAATGATT
TATCAAATCCACCAACAGATGAAGAATTAAATAACTATAAGGATTTACCAGCATCAAGTC
TTCCACCAATTCATAAAAGAGAGAAATTAAGAAGAGTATTTGTAAATAGAGATATTAAAC
TCGACAGAATTGAATTCTTTGGTTTTGATATGGATTATACATTAGCAGTTTATAATTCAC
CAGATTTTGAAGAGTTGGCATATGATATGGTACTTGATAAACTCATTGATATTGGTTATC
CAAAATCAATTAGAAAATTAAAATATGATCCAAATTTCCCAACTAGAGGTTTATTTTTAG
ATAGAGAACTTGGTAACCTTTTAAAAATTGATAGTTTTGGTAACATTATCAXXXXXXXXX
XTATTACACAAATAAAGTTATGTCATATATGATGAATGGATATAATCCAAACTATCAATC
ATGGAGAGATTATTTTGATGTTATTATTGTTGGTGCAGATAAACCAAGATTCTTTTCAGA
AGGTACAACAATTAGAGAAGTTGATACTGAAACTGGTAATTTAAGAATTACAAATGTAAA
GGATAGATTTGAACAAGGTAAAGTTTATCATGGTGGTTCTTTATCATTATTCCAAAAATT
AACAGGAGCAAAAGGTAGTAGAGTACTTTACATTGGTGATCATATCTTTGCAGATATCAT
TAAATCAAAGAAAACTCATGGATGGAGAAATCTATTGGTTGTACCAGAACTTCAACATGA
ATTAGAGGTTATGAATCAACAAAAAGATACAACCATTCATTTGATGAATCTTGAATTCAT
TAGAGCTGAAATCTATAGAGGCTTAGATTCTGAATCAACCACCCCACCAGATATTGAAGT
TCTCCATCAACATCTTAAAAATACAAATGATAAATTGAACTTTGCCTACAACAAATACTT
TGGTTCCCTCTTTAAAAATGGTTCAAAATCAACTTTCTTCTCTATGCAAGTTCAAAGATA
TGCTGATCTTTACACCTCNGATTATCTCAATCTCTTAAACTATCCATTATTTTATCATTT
TTGTGCAAAAGTTTACCTCTTCCACANAAAGTTCTTCTTTCTCTTCTTTNATACAAGAAT
TAATAAATAAAGAAAANAAA

KIQIINNMAENNNNNNNNNNNNVSTPPHQKPHLTTGLRTSSSGLLMDKRRQDEEKFSAEQ
VAMGKKSPTKLFSAYSLNDLSNPPTDEELNNYKDLPASSLPPIHKREKLRRVFVNRDIKL
DRIEFFGFDMDYTLAVYNSPDFEELAYDMVLDKLIDIGYPKSIRKLKYDPNFPTRGLFLD
RELGNLLKIDSFGNII---

---YYTNKVMSYMMNGYNPNYQSWRDYFDVIIVGADKPRFFSEGTTIREVDTETGNLRIT
NVKDRFEQGKVYHGGSLSLFQKLTGAKGSRVLYIGDHIFADIIKSKKTHGWRNLLVVPEL
QHELEVMNQQKDTTIHLMNLEFIRAEIYRGLDSESTTPPDIEVLHQHLKNTNDKLNFAYN
KYFGSLFKNGSKSTFFSMQVQRYADLYTSDYLNLLNYPLFYHFCAKVYLFHXKFFFLFFX
TRINK*rkx

Frame A:
lkfklsttwqkiiiiiiiiiiimfqhhhiknhi*qlv*elqvlvy*wikedkmkknfqln
k*qwvknhqqnysqhta*miyqihqqmkn*itiriyqhqvfhqfikern*eeyl*ieiln
stelnslvliwiih*qfiihqilkswhmiwylinslilviqnqlen*nmiqisqlevyf*
ienlvtf*klivlvtls---

---YYTNKVMSYMMNGYNPNYQSWRDYFDVIIVGADKPRFFSEGTTIREVDTETGNLRIT
NVKDRFEQGKVYHGGSLSLFQKLTGAKGSRVLYIGDHIFADIIKSKKTHGWRNLLVVPEL
QHELEVMNQQKDTTIHLMNLEFIRAEIYRGLDSESTTPPDIEVLHQHLKNTNDKLNFAYN
KYFGSLFKNGSKSTFFSMQVQRYADLYTSDYLNLLNYPLFYHFCAKVYLFHXKFFFLFFX
TRINK*rkx

Frame B:
*nsnyqqhgrk***********cfntttskttfnnwfknfkfwfing*kktr*rkifs*t
ssng*kitnkiilsiqlk*fikstnr*rik*l*gftsiksstns*kreikksick*ry*t
rqn*ilwf*yglyissl*ftrf*rvgi*ygt**th*ywlskin*kiki*skfpn*rfifr
*rtw*pfkn**fw*hy---

---itqiklchi**mdiiqtinhgeiilmllllvqinqdsfqkvqqleklilklvi*elq
m*ridlnkvkfimvvlyhyskn*qeqkvveyftlviislqislnqrklmdgeiywlyqnf
nmn*rl*inkkiqpfi**ilnslelksiea*ilnqpphqilkfsinilkiqmin*tlptt
ntlvpslkmvqnqlsslckfkdmliftpxiisis*tihyfiifvqkftsstxsssfssxi
qelinkexk

Frame C:
KIQIINNMAENNNNNNNNNNNNVSTPPHQKPHLTTGLRTSSSGLLMDKRRQDEEKFSAEQ
VAMGKKSPTKLFSAYSLNDLSNPPTDEELNNYKDLPASSLPPIHKREKLRRVFVNRDIKL
DRIEFFGFDMDYTLAVYNSPDFEELAYDMVLDKLIDIGYPKSIRKLKYDPNFPTRGLFLD
RELGNLLKIDSFGNII---

---lhk*syviydewi*sklsimerlf*cyycwcr*tkilfrrynn*rs*y*nw*fknyk
ckg*i*tr*slswwffiiipkinrskr**stlhw*sylcryh*ikenswmeksigctrts
t*irgyestkrynhsfdes*ih*s*nl*rlrf*inhptry*sspsts*kyk**ielclqq
ilwfpl*kwfkinfllyasskic*slhlxlsqslklsiilsflckslplpxkvllsllxy
kn**ikkx

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ789 (AFJ789Q) /CSM/AF/AFJ7-D/AFJ789Q.Seq.d/                       2438   0.0
VFH524 (VFH524Q) /CSM/VF/VFH5-A/VFH524Q.Seq.d/                       1388   0.0
AFJ814 (AFJ814Q) /CSM/AF/AFJ8-A/AFJ814Q.Seq.d/                       1386   0.0
SFJ610 (SFJ610Q) /CSM/SF/SFJ6-A/SFJ610Q.Seq.d/                       1378   0.0
VHH636 (VHH636Q) /CSM/VH/VHH6-B/VHH636Q.Seq.d/                       1368   0.0
AFF109 (AFF109Q) /CSM/AF/AFF1-A/AFF109Q.Seq.d/                       1354   0.0
SFF696 (SFF696Q) /CSM/SF/SFF6-D/SFF696Q.Seq.d/                       1342   0.0
CFI768 (CFI768Q) /CSM/CF/CFI7-C/CFI768Q.Seq.d/                       1340   0.0
CFK414 (CFK414Q) /CSM/CF/CFK4-A/CFK414Q.Seq.d/                       1326   0.0
SFE254 (SFE254Q) /CSM/SF/SFE2-C/SFE254Q.Seq.d/                       1310   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    44  0.006  12
AC120206|AC120206.2 Canis familiaris clone RP81-219C14, WORKING DRAFT SEQUENCE, 7 ordered pieces.    56  0.012  3
CC008913|CC008913.1 PUDEI80TD ZM_0.6_1.0_KB Zea mays genomic clone ZMMBTa168M15, DNA sequence.    44  0.050  2
BB845391|BB845391.1 Mus musculus adult male kidney cDNA, RIKEN full-length enriched library, clone:F520005H13, 5' end partial sequence.    46  0.067  2
AC108322|AC108322.4 Rattus norvegicus clone CH230-261G16, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    50  0.074  1
BB392891|BB392891.1 Mus musculus 0 day neonate cerebellum cDNA, RIKEN full-length enriched library, clone:C230081A07, 3' end partial sequence.    50  0.074  1
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    36  0.11  15
AC114263|AC114263.2 Dictyostelium discoideum chromosome 2 map 215673-367476 strain AX4, complete sequence.    34  0.14  9
AC117531|AC117531.2 Homo sapiens chromosome 5 clone RP11-603O17, complete sequence.    42  0.15  6
AC035141|AC035141.3 Homo sapiens chromosome 5 clone CTD-2010H15, WORKING DRAFT SEQUENCE, 10 ordered pieces.    44  0.18  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54XC1) RecName: Full=Cytosolic purine 5'-nucleotidase;        ...   461   e-128
AE014298_2834(AE014298|pid:none) Drosophila melanogaster chromos...   208   4e-52
AE014298_2835(AE014298|pid:none) Drosophila melanogaster chromos...   208   4e-52
AE014298_2830(AE014298|pid:none) Drosophila melanogaster chromos...   208   4e-52
AE014298_2833(AE014298|pid:none) Drosophila melanogaster chromos...   208   4e-52
BC064760_1(BC064760|pid:none) Mus musculus 5'-nucleotidase, cyto...   202   3e-50
AK036730_1(AK036730|pid:none) Mus musculus adult male bone cDNA,...   202   3e-50
AK132384_1(AK132384|pid:none) Mus musculus 15 days embryo head c...   202   3e-50
(Q3V1L4) RecName: Full=Cytosolic purine 5'-nucleotidase;        ...   202   3e-50
AK291667_1(AK291667|pid:none) Homo sapiens cDNA FLJ75877 complet...   202   3e-50

 psg: 0.67  gvh: 0.39  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.07
 nuc: 0.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for AFJ789 is cyt

>AFJ789F.Seq
TTAAAATTCAAATTATCAACAACATGGCAGAAAATAATAATAATAATAATAATAATAATA
ATAATAATGTTTCAACACCACCACATCAAAAACCACATTTAACAACTGGTTTAAGAACTT
CAAGTTCTGGTTTATTAATGGATAAAAGAAGACAAGATGAAGAAAAATTTTCAGCTGAAC
AAGTAGCAATGGGTAAAAAATCACCAACAAAATTATTCTCAGCATACAGCTTAAATGATT
TATCAAATCCACCAACAGATGAAGAATTAAATAACTATAAGGATTTACCAGCATCAAGTC
TTCCACCAATTCATAAAAGAGAGAAATTAAGAAGAGTATTTGTAAATAGAGATATTAAAC
TCGACAGAATTGAATTCTTTGGTTTTGATATGGATTATACATTAGCAGTTTATAATTCAC
CAGATTTTGAAGAGTTGGCATATGATATGGTACTTGATAAACTCATTGATATTGGTTATC
CAAAATCAATTAGAAAATTAAAATATGATCCAAATTTCCCAACTAGAGGTTTATTTTTAG
ATAGAGAACTTGGTAACCTTTTAAAAATTGATAGTTTTGGTAACATTATCA---------
-

>AFJ789Z.Seq
----------TATTACACAAATAAAGTTATGTCATATATGATGAATGGATATAATCCAAA
CTATCAATCATGGAGAGATTATTTTGATGTTATTATTGTTGGTGCAGATAAACCAAGATT
CTTTTCAGAAGGTACAACAATTAGAGAAGTTGATACTGAAACTGGTAATTTAAGAATTAC
AAATGTAAAGGATAGATTTGAACAAGGTAAAGTTTATCATGGTGGTTCTTTATCATTATT
CCAAAAATTAACAGGAGCAAAAGGTAGTAGAGTACTTTACATTGGTGATCATATCTTTGC
AGATATCATTAAATCAAAGAAAACTCATGGATGGAGAAATCTATTGGTTGTACCAGAACT
TCAACATGAATTAGAGGTTATGAATCAACAAAAAGATACAACCATTCATTTGATGAATCT
TGAATTCATTAGAGCTGAAATCTATAGAGGCTTAGATTCTGAATCAACCACCCCACCAGA
TATTGAAGTTCTCCATCAACATCTTAAAAATACAAATGATAAATTGAACTTTGCCTACAA
CAAATACTTTGGTTCCCTCTTTAAAAATGGTTCAAAATCAACTTTCTTCTCTATGCAAGT
TCAAAGATATGCTGATCTTTACACCTCNGATTATCTCAATCTCTTAAACTATCCATTATT
TTATCATTTTTGTGCAAAAGTTTACCTCTTCCACANAAAGTTCTTCTTTCTCTTCTTTNA
TACAAGAATTAATAAATAAAGAAAANAAA

>AFJ789P.Seq
TTAAAATTCAAATTATCAACAACATGGCAGAAAATAATAATAATAATAATAATAATAATA
ATAATAATGTTTCAACACCACCACATCAAAAACCACATTTAACAACTGGTTTAAGAACTT
CAAGTTCTGGTTTATTAATGGATAAAAGAAGACAAGATGAAGAAAAATTTTCAGCTGAAC
AAGTAGCAATGGGTAAAAAATCACCAACAAAATTATTCTCAGCATACAGCTTAAATGATT
TATCAAATCCACCAACAGATGAAGAATTAAATAACTATAAGGATTTACCAGCATCAAGTC
TTCCACCAATTCATAAAAGAGAGAAATTAAGAAGAGTATTTGTAAATAGAGATATTAAAC
TCGACAGAATTGAATTCTTTGGTTTTGATATGGATTATACATTAGCAGTTTATAATTCAC
CAGATTTTGAAGAGTTGGCATATGATATGGTACTTGATAAACTCATTGATATTGGTTATC
CAAAATCAATTAGAAAATTAAAATATGATCCAAATTTCCCAACTAGAGGTTTATTTTTAG
ATAGAGAACTTGGTAACCTTTTAAAAATTGATAGTTTTGGTAACATTATCA---------
-TATTACACAAATAAAGTTATGTCATATATGATGAATGGATATAATCCAAACTATCAATC
ATGGAGAGATTATTTTGATGTTATTATTGTTGGTGCAGATAAACCAAGATTCTTTTCAGA
AGGTACAACAATTAGAGAAGTTGATACTGAAACTGGTAATTTAAGAATTACAAATGTAAA
GGATAGATTTGAACAAGGTAAAGTTTATCATGGTGGTTCTTTATCATTATTCCAAAAATT
AACAGGAGCAAAAGGTAGTAGAGTACTTTACATTGGTGATCATATCTTTGCAGATATCAT
TAAATCAAAGAAAACTCATGGATGGAGAAATCTATTGGTTGTACCAGAACTTCAACATGA
ATTAGAGGTTATGAATCAACAAAAAGATACAACCATTCATTTGATGAATCTTGAATTCAT
TAGAGCTGAAATCTATAGAGGCTTAGATTCTGAATCAACCACCCCACCAGATATTGAAGT
TCTCCATCAACATCTTAAAAATACAAATGATAAATTGAACTTTGCCTACAACAAATACTT
TGGTTCCCTCTTTAAAAATGGTTCAAAATCAACTTTCTTCTCTATGCAAGTTCAAAGATA
TGCTGATCTTTACACCTCNGATTATCTCAATCTCTTAAACTATCCATTATTTTATCATTT
TTGTGCAAAAGTTTACCTCTTCCACANAAAGTTCTTCTTTCTCTTCTTTNATACAAGAAT
TAATAAATAAAGAAAANAAA