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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLA546
Library SL
(Link to library)
Clone ID SLA546
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16394-1
Original site URL
Representative seq. ID SLA546F
(Link to Original site)
Representative DNA sequence 
>SLA546 (SLA546Q) /CSM/SL/SLA5-B/SLA546Q.Seq.d/
AGAGTGCTAGTGGCAACAACAACAACAATAACAACAGCAATAATAATAGTAATAATAATA
GTACTTCTCCATCTGCAAGCTCTTCAAAGAGTCCATTATTATTCAGTGGTTCAGATGATC
CTTTCAAGATATCTCCACTTCCAAATCCATTTGGTGGAAATAATTATAAAGTTAATTCTC
AAACCAATCCAGGTTTCTCAATTACAAATAATAGTAGCGTTAAAGTTCCATCAGAATTGT
TAAAATCTTCAATTCATAATCATCATTTACAAGTTCAACAACAACATTTCTTAAATCATC
ATCAACAACATAATAATAATAACCAACAAGCACAACAACATATAAATAATAATAATAATA
ATAATAACAGCAATAATAATAATAGCGATGAAGATGTTGTTGAAAATAATAGTGGTAATC
CACCACCATCACCAAATAGTGCCAAACTCACTAAAATCCAACAACAAAATTTAATCTTAA
ATTGTACATATTAXXXXXXXXXX
sequence update 1999. 2.19
Translated Amino Acid sequence 
SASGNNNNNNNSNNNSNNNSTSPSASSSKSPLLFSGSDDPFKISPLPNPFGGNNYKVNSQ
TNPGFSITNNSSVKVPSELLKSSIHNHHLQVQQQHFLNHHQQHNNNNQQAQQHINNNNNN
NNSNNNNSDEDVVENNSGNPPPSPNSAKLTKIQQQNLILNCTY---
Translated Amino Acid sequence (All Frames) 
Frame A:
rvlvattttittaiiiviiivllhlqalqrvhyysvvqmilsrylhfqihlveiiiklil
kpiqvsqlqiivalkfhqnc*nlqfiiiiykfnnnis*iiinniiiitnkhnni*iiiii
iitaiiiiamkmllkiivvihhhhqivpnslksnnki*s*ivhi---


Frame B:
ec*wqqqqq*qqq*******yfsicklfkesiiiqwfr*sfqdistsksiwwk*l*s*fs
nqsrflnyk***r*ssirivkifns*ssftsstttflkssstt****ptstttyk*****
**qq****r*rcc*k**w*sttitk*cqth*npttkfnlklyi---


Frame C:
SASGNNNNNNNSNNNSNNNSTSPSASSSKSPLLFSGSDDPFKISPLPNPFGGNNYKVNSQ
TNPGFSITNNSSVKVPSELLKSSIHNHHLQVQQQHFLNHHQQHNNNNQQAQQHINNNNNN
NNSNNNNSDEDVVENNSGNPPPSPNSAKLTKIQQQNLILNCTY---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLH468 (SLH468Q) /CSM/SL/SLH4-C/SLH468Q.Seq.d/                        505   e-142
SLA546 (SLA546Q) /CSM/SL/SLA5-B/SLA546Q.Seq.d/                        505   e-142
SSM579 (SSM579Q) /CSM/SS/SSM5-D/SSM579Q.Seq.d/                        498   e-140
SLB655 (SLB655Q) /CSM/SL/SLB6-C/SLB655Q.Seq.d/                        216   2e-55
AFM131 (AFM131Q) /CSM/AF/AFM1-B/AFM131Q.Seq.d/                         38   0.12
VHL476 (VHL476Q) /CSM/VH/VHL4-D/VHL476Q.Seq.d/                         36   0.49
VHL122 (VHL122Q) /CSM/VH/VHL1-A/VHL122Q.Seq.d/                         36   0.49
VFB430 (VFB430Q) /CSM/VF/VFB4-B/VFB430Q.Seq.d/                         36   0.49
SSL388 (SSL388Q) /CSM/SS/SSL3-D/SSL388Q.Seq.d/                         36   0.49
SSG471 (SSG471Q) /CSM/SS/SSG4-C/SSG471Q.Seq.d/                         36   0.49
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU060192) Dictyostelium discoideum slug cDNA, clone SLA546.      424   e-172  3
(AU040023) Dictyostelium discoideum slug cDNA, clone SLH468.      424   e-171  2
(AC117073) Dictyostelium discoideum chromosome 2 map 6045872...   424   e-170  2
(C93122) Dictyostelium discoideum slug cDNA, clone SSM579.        416   e-169  2
(AU033955) Dictyostelium discoideum slug cDNA, clone SLB655.      216   6e-52  1
(CU928847) Zebrafish DNA sequence from clone CH73-103B11 in ...    48   0.31   1
(CU929401) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS...    48   0.31   1
(GD812323) 454GmaGlobSeed554972 Soybean Seeds Containing Glo...    48   0.31   1
(ET422851) S0124K11_SP6 CHORI-214 Salmo salar genomic clone ...    36   0.88   2
(AC116982) Dictyostelium discoideum chromosome 2 map 3622643...    36   1.1    6
dna update 2009. 1.25
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3101297
Number of Hits to DB: 466,506,293
protein update 2009. 5.31
PSORT 

 psg: 0.81  gvh: 0.34  alm: 0.57  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: mitochondrial
	 28.0 %: nuclear
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for SLA546 is cyt
5' end seq. ID SLA546F
5' end seq. 
>SLA546F.Seq
AGAGTGCTAGTGGCAACAACAACAACAATAACAACAGCAATAATAATAGTAATAATAATA
GTACTTCTCCATCTGCAAGCTCTTCAAAGAGTCCATTATTATTCAGTGGTTCAGATGATC
CTTTCAAGATATCTCCACTTCCAAATCCATTTGGTGGAAATAATTATAAAGTTAATTCTC
AAACCAATCCAGGTTTCTCAATTACAAATAATAGTAGCGTTAAAGTTCCATCAGAATTGT
TAAAATCTTCAATTCATAATCATCATTTACAAGTTCAACAACAACATTTCTTAAATCATC
ATCAACAACATAATAATAATAACCAACAAGCACAACAACATATAAATAATAATAATAATA
ATAATAACAGCAATAATAATAATAGCGATGAAGATGTTGTTGAAAATAATAGTGGTAATC
CACCACCATCACCAAATAGTGCCAAACTCACTAAAATCCAACAACAAAATTTAATCTTAA
ATTGTACATATTA----------
Length of 5' end seq. 493
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -