NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK405
Library AF
(Link to library)
Clone ID AFK405
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U15477-1
Original site URL
Representative seq. ID AFK405P
(Link to Original site)
Representative DNA sequence 
>AFK405 (AFK405Q) /CSM/AF/AFK4-A/AFK405Q.Seq.d/
TATTATTGTTAAACAATAAATCAAATTAAAAAATGACTTTATCAGTGGAACAAATTTCAA
AATTGGAGGAGTCTAAAATAAGAGCCAAGAAAGTGTATGAAAATTATGATGAAAAATTAA
ATAATTTTTTAAATGAACAATTGGTTGAATTAAAGAAACAAAACAACTCAATTTCAAATT
TTAGTGAGAATGAAATTAAAGATTTGAAAATTTCATTCCAATCAATTCAATCTGAAAAAA
GTGCCAATGAACTTTACAGTGAATTCCCATTTGAAGAAATGTGGGCAATAATGGGTCTTG
CATTGAGTGCTGATCCATCAGGTGTTGCTCCAATTATTTTCGGTGCTATTAATATTCTTT
TTAATAGTCTCGGTTTATTTAATTCCCAAGAAAGATTCTTTAAACAAATAATGGAAGCAG
TTGAAAAATTAATTGACCATAAGTTAAGTCAAGAAAGAGAGAGACAATGTATAATTAAAT
TCCAACAACTTCAAAAAACTGGCGATGACTATTTTGTATTAGCTGAAGAATATTTTCAAG
AAATGGAAATAAAAATGTTAGTCGTAGTATTATTCCAAATGATATTCAAGTAATGACTAA
TGAACAACTTAATTCTTCACTTCAATTTCAATTAATGATTTATCGTXXXXXXXXXXCCCG
TCATATGCATAGCGAAGAAACTTCCCCAATTTTTGGTTATTATAGACCACCATACTTTGA
TGTTGTAAGAAAATTCAAAAATGCAGACTTTACCTATTATCCTCATAGAGCATTTGATTA
CCAAAGAAATGTTGGTGAAATTCATTTTAATTGTGGTGATGAAAGAGGTTCCCATTTACT
TTACCCAGGTTTAATTGTAGGGTTTTCTCAAGTTACCAATAGTTTATCTTCTGGGTTTAA
AGGACTTACCGCAGGAACTTTAAATTTACAAGTAACTTTGATTGTAAAGTTTGAAAGTAT
TAAAAAAAGATCATTTAAAGTTAAGATACATCATCATATTAATGGCAATGCTACGTGGGT
TGTTGAAAGTGGTCCTGTCTCAGAAGTGTTAAGTTTTAATCAAGGTACTGAAAAAGAATT
TAGAAACTCTTACTTTGTTTATAATGAAAGTTTGCAAAATCCACCACATGCTACTAATGA
ATCCAAGCTTATTACAGTAGAAAATGAACAAGTCACATTTAAATGTATTAGATCTATGAA
TGGTGGTACAAAAACTGGGTATGATGCAGGTTCAAAACTATATTTAATTGAACTTATTTT
TGATTAATGGAAACACAAAGGCCTCTTGGTTGTAGATNATTTGATATGTACTCATACATC
CTATTAGTCCATTAACTAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
iivkq*iklkndfisgtnfkiggv*nksqesv*kl**kik*ffk*tig*iketkqlnfkf
**e*n*rfenfipinsi*kkcq*tlq*ipi*rnvgnngsciec*sirccsnyfrcy*ysf
**srfi*fprkil*tnngss*kin*p*vksrkretmyn*ipttsknwr*lfcis*RIFSR
NGNKNVSRSIIPNDIQVMTNEQLNSSLQFQLMIYR---

---RHMHSEETSPIFGYYRPPYFDVVRKFKNADFTYYPHRAFDYQRNVGEIHFNCGDERG
SHLLYPGLIVGFSQVTNSLSSGFKGLTAGTLNLQVTLIVKFESIKKRSFKVKIHHHINGN
ATWVVESGPVSEVLSFNQGTEKEFRNSYFVYNESLQNPPHATNESKLITVENEQVTFKCI
RSMNGGTKTGYDAGSKLYLIELIFD*wkhkgllvvdxlicthtsy*sin*
Translated Amino Acid sequence (All Frames) 
Frame A:
yyc*tinqikk*lyqwnkfqnwrslk*eprkcmkimmkn*iif*mnnwln*rnkttqfqi
lvrmklki*kfhsnqfnlkkvpmnftvnshlkkcgq*wvlh*vlihqvllqlfsvlliff
livsvylipkkdslnk*wkqlkn*ltis*vkkerdnv*lnsnnfkklamtily*lknifk
kwk*kc*s*yysk*yssnd**tt*fftsisindls---

---pviciakklpqflviidhhtlml*enskmqtlpiiliehlitkemlvkfilivvmke
vpiyftqv*l*gflklpivyllglkdlpqel*iyk*l*l*slkvlkkdhlklryiiilma
mlrgllkvvlsqkc*vlikvlkknletltlfimkvckihhmllmnpsllq*kmnkshlnv
ldl*mvvqklgmmqvqnyi*lnlflingntkaswl*xi*yvlihpispltk

Frame B:
iivkq*iklkndfisgtnfkiggv*nksqesv*kl**kik*ffk*tig*iketkqlnfkf
**e*n*rfenfipinsi*kkcq*tlq*ipi*rnvgnngsciec*sirccsnyfrcy*ysf
**srfi*fprkil*tnngss*kin*p*vksrkretmyn*ipttsknwr*lfcis*RIFSR
NGNKNVSRSIIPNDIQVMTNEQLNSSLQFQLMIYR---

---psya*rrnfpnfwll*ttil*cckkiqkcrlyllss*si*lpkkcw*nsf*lw**kr
fpftlprfncrvfssyq*fifwv*rtyrrnfkftsnfdckv*ky*kkii*s*dtssy*wq
cyvgc*kwsclrsvkf*sry*kri*klllcl**kfaksttcy**iqayysrk*tshi*my
*iyewwyknwv*crfktifn*tyf*lmetqrplgcrxfdmysyillvh*lk

Frame C:
lllnnksn*kmtlsveqiskleeskirakkvyenydeklnnflneqlvelkkqnnsisnf
seneikdlkisfqsiqseksanelysefpfeemwaimglalsadpsgvapiifgainilf
nslglfnsqerffkqimeaveklidhklsqererqciikfqqlqktgddyfvlaeeyfqe
meikmlvvvlfqmifk**lmnnlilhfnfn**fi---

---RHMHSEETSPIFGYYRPPYFDVVRKFKNADFTYYPHRAFDYQRNVGEIHFNCGDERG
SHLLYPGLIVGFSQVTNSLSSGFKGLTAGTLNLQVTLIVKFESIKKRSFKVKIHHHINGN
ATWVVESGPVSEVLSFNQGTEKEFRNSYFVYNESLQNPPHATNESKLITVENEQVTFKCI
RSMNGGTKTGYDAGSKLYLIELIFD*wkhkgllvvdxlicthtsy*sin*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK405 (AFK405Q) /CSM/AF/AFK4-A/AFK405Q.Seq.d/                       2551   0.0
VFG293 (VFG293Q) /CSM/VF/VFG2-D/VFG293Q.Seq.d/                       1191   0.0
AFK554 (AFK554Q) /CSM/AF/AFK5-C/AFK554Q.Seq.d/                       1189   0.0
AFE736 (AFE736Q) /CSM/AF/AFE7-B/AFE736Q.Seq.d/                       1156   0.0
AFL201 (AFL201Q) /CSM/AF/AFL2-A/AFL201Q.Seq.d/                       1148   0.0
VFG421 (VFG421Q) /CSM/VF/VFG4-A/VFG421Q.Seq.d/                       1102   0.0
VFK782 (VFK782Q) /CSM/VF/VFK7-D/VFK782Q.Seq.d/                       1094   0.0
VFH265 (VFH265Q) /CSM/VF/VFH2-C/VFH265Q.Seq.d/                       1092   0.0
VFD530 (VFD530Q) /CSM/VF/VFD5-B/VFD530Q.Seq.d/                       1084   0.0
VFO730 (VFO730Q) /CSM/VF/VFO7-B/VFO730Q.Seq.d/                       1082   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.   690   0.0    18
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.   151   3e-32  1
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           40   4e-13  28
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          40   3e-12  34
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.            38   7e-09  30
CR382134|CR382134.1 Debaryomyces hansenii chromosome B of strain CBS767 of Debaryomyces hansenii.    42   8e-08  26
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    36   9e-08  22
AE017339|AE017339.1 Mycoplasma mobile 163K section 3 of 3 of the complete genome.    32   1e-07  20
AL929353|AL929353.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 3/4.    38   1e-06  20
AE014817|AE014817.1 Plasmodium falciparum 3D7 chromosome 14 section 2 of 13 of the complete sequence.    46   2e-06  17
dna update 2004. 7.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117075_61(AC117075|pid:none) Dictyostelium discoideum chromoso...   355   3e-96
AC117075_62(AC117075|pid:none) Dictyostelium discoideum chromoso...   194   6e-48
(P11872) RecName: Full=cAMP-regulated M3R protein; AltName: Full...    41   0.081
CP000812_905(CP000812|pid:none) Thermotoga lettingae TMO, comple...    37   1.5
AC117072_17(AC117072|pid:none) Dictyostelium discoideum chromoso...    35   7.6
protein update 2008.11.25
PSORT 

 psg: 0.68  gvh: 0.25  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.50  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 20.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum

>> prediction for AFK405 is mit
5' end seq. ID AFK405F
5' end seq. 
>AFK405F.Seq
TATTATTGTTAAACAATAAATCAAATTAAAAAATGACTTTATCAGTGGAACAAATTTCAA
AATTGGAGGAGTCTAAAATAAGAGCCAAGAAAGTGTATGAAAATTATGATGAAAAATTAA
ATAATTTTTTAAATGAACAATTGGTTGAATTAAAGAAACAAAACAACTCAATTTCAAATT
TTAGTGAGAATGAAATTAAAGATTTGAAAATTTCATTCCAATCAATTCAATCTGAAAAAA
GTGCCAATGAACTTTACAGTGAATTCCCATTTGAAGAAATGTGGGCAATAATGGGTCTTG
CATTGAGTGCTGATCCATCAGGTGTTGCTCCAATTATTTTCGGTGCTATTAATATTCTTT
TTAATAGTCTCGGTTTATTTAATTCCCAAGAAAGATTCTTTAAACAAATAATGGAAGCAG
TTGAAAAATTAATTGACCATAAGTTAAGTCAAGAAAGAGAGAGACAATGTATAATTAAAT
TCCAACAACTTCAAAAAACTGGCGATGACTATTTTGTATTAGCTGAAGAATATTTTCAAG
AAATGGAAATAAAAATGTTAGTCGTAGTATTATTCCAAATGATATTCAAGTAATGACTAA
TGAACAACTTAATTCTTCACTTCAATTTCAATTAATGATTTATCGT----------
Length of 5' end seq. 646
3' end seq. ID AFK405Z
3' end seq. 
>AFK405Z.Seq
----------CCCGTCATATGCATAGCGAAGAAACTTCCCCAATTTTTGGTTATTATAGA
CCACCATACTTTGATGTTGTAAGAAAATTCAAAAATGCAGACTTTACCTATTATCCTCAT
AGAGCATTTGATTACCAAAGAAATGTTGGTGAAATTCATTTTAATTGTGGTGATGAAAGA
GGTTCCCATTTACTTTACCCAGGTTTAATTGTAGGGTTTTCTCAAGTTACCAATAGTTTA
TCTTCTGGGTTTAAAGGACTTACCGCAGGAACTTTAAATTTACAAGTAACTTTGATTGTA
AAGTTTGAAAGTATTAAAAAAAGATCATTTAAAGTTAAGATACATCATCATATTAATGGC
AATGCTACGTGGGTTGTTGAAAGTGGTCCTGTCTCAGAAGTGTTAAGTTTTAATCAAGGT
ACTGAAAAAGAATTTAGAAACTCTTACTTTGTTTATAATGAAAGTTTGCAAAATCCACCA
CATGCTACTAATGAATCCAAGCTTATTACAGTAGAAAATGAACAAGTCACATTTAAATGT
ATTAGATCTATGAATGGTGGTACAAAAACTGGGTATGATGCAGGTTCAAAACTATATTTA
ATTGAACTTATTTTTGATTAATGGAAACACAAAGGCCTCTTGGTTGTAGATNATTTGATA
TGTACTCATACATCCTATTAGTCCATTAACTAAAA
Length of 3' end seq. 685
Connected seq. ID AFK405P
Connected seq. 
>AFK405P.Seq
TATTATTGTTAAACAATAAATCAAATTAAAAAATGACTTTATCAGTGGAACAAATTTCAA
AATTGGAGGAGTCTAAAATAAGAGCCAAGAAAGTGTATGAAAATTATGATGAAAAATTAA
ATAATTTTTTAAATGAACAATTGGTTGAATTAAAGAAACAAAACAACTCAATTTCAAATT
TTAGTGAGAATGAAATTAAAGATTTGAAAATTTCATTCCAATCAATTCAATCTGAAAAAA
GTGCCAATGAACTTTACAGTGAATTCCCATTTGAAGAAATGTGGGCAATAATGGGTCTTG
CATTGAGTGCTGATCCATCAGGTGTTGCTCCAATTATTTTCGGTGCTATTAATATTCTTT
TTAATAGTCTCGGTTTATTTAATTCCCAAGAAAGATTCTTTAAACAAATAATGGAAGCAG
TTGAAAAATTAATTGACCATAAGTTAAGTCAAGAAAGAGAGAGACAATGTATAATTAAAT
TCCAACAACTTCAAAAAACTGGCGATGACTATTTTGTATTAGCTGAAGAATATTTTCAAG
AAATGGAAATAAAAATGTTAGTCGTAGTATTATTCCAAATGATATTCAAGTAATGACTAA
TGAACAACTTAATTCTTCACTTCAATTTCAATTAATGATTTATCGT----------CCCG
TCATATGCATAGCGAAGAAACTTCCCCAATTTTTGGTTATTATAGACCACCATACTTTGA
TGTTGTAAGAAAATTCAAAAATGCAGACTTTACCTATTATCCTCATAGAGCATTTGATTA
CCAAAGAAATGTTGGTGAAATTCATTTTAATTGTGGTGATGAAAGAGGTTCCCATTTACT
TTACCCAGGTTTAATTGTAGGGTTTTCTCAAGTTACCAATAGTTTATCTTCTGGGTTTAA
AGGACTTACCGCAGGAACTTTAAATTTACAAGTAACTTTGATTGTAAAGTTTGAAAGTAT
TAAAAAAAGATCATTTAAAGTTAAGATACATCATCATATTAATGGCAATGCTACGTGGGT
TGTTGAAAGTGGTCCTGTCTCAGAAGTGTTAAGTTTTAATCAAGGTACTGAAAAAGAATT
TAGAAACTCTTACTTTGTTTATAATGAAAGTTTGCAAAATCCACCACATGCTACTAATGA
ATCCAAGCTTATTACAGTAGAAAATGAACAAGTCACATTTAAATGTATTAGATCTATGAA
TGGTGGTACAAAAACTGGGTATGATGCAGGTTCAAAACTATATTTAATTGAACTTATTTT
TGATTAATGGAAACACAAAGGCCTCTTGGTTGTAGATNATTTGATATGTACTCATACATC
CTATTAGTCCATTAACTAAAA
Length of connected seq. 1331
Full length Seq ID -
Full length Seq. -
Length of full length seq. -