>AFK789 (AFK789Q) /CSM/AF/AFK7-D/AFK789Q.Seq.d/
TTATTCATATAAATTTTAATATTAAAATTAAAATATGAAATTAAATTATTTAATTTCTAT
ATTATTATTTTTAATAATAAATTATTCATCATTTGTAAGTAGTATAAACATTTTTAAAGA
TATCAATATAATCTATGATCATTTAAAGGATGACATAAAAGAGATTATTGTTGATGCAAC
TACAACAGTGAATCATGTAGAATACTTTATTGATATAGTGAATAGCAATGGATACCCATG
TGAGCATCATTCAGTTATAACAGAAGATGGTTATATATTGGGTGTATTTAGAATTCCATA
TTCTTATAACAATAATCAAAATCTTAATAATAAAACTAGACAACCAATTCTATTGCAACA
TGGTTTATTAGACTCATCGATAACATGGATAGTTAATAATGCAAATCAATCACTACCATT
CATATTATCAGATATGGGATACGATGTATGGATGGGTAATAACCCGTGGTAATACATTTA
GTATCAATCATACTAGATTGGATGTAAAGAGTAGAGAATTTTGGGAATTCTCATTCGATG
ATATGGGGTTGGTACGATCTACCATCAATGGTTGATTATATCAXXXXXXXXXXATGGTTA
TNTGGNCCNCANAAAGGNGCCTTCAATCAATCAANAANGCCATTTGTAAGTGGNAATGAA
CCTGGNGGNACTTCNCTTANAAANATGGNCCNTTTCNCNCAATTAGNAAATTCAAAACNA
TTTCAACNTTATGATTANGGAGNTATTGGNAATTTANTACNTTATGGTCATGAAAAACCC
CCNTTAATTAATGTTNAAAANANACCCCCNACTGNAAANATTGCNTTATTNTCTGGGACA
AAGGATGAATTGGCAGACCCNANANATGNAAAGCNATTAGNATCNCTCTTACCNCCTGAA
ACNAAACNCNCTTGGGACANAATTGAAAANTANGCNCNTTTAGATTTTGNTTGGGCNA

lfi*ililklkyeiklfnfyiiifnnklfiick*ykhf*ryqynl*sfkg*hkrdyc*cn
ynsescrily*yse*qwipm*asfsynrrwlyigci*nsifl*q*sks***n*ttnsiat
wfirlidnmds**cksittihiirygircmdg**pvvihlvsiildwm*RVENFGNSHSM
IWGWYDLPSMVDYI---

---WLXGPXKGAFNQSXXPFVSGNEPGGTSLXXMXXFXQLXNSKXFQXYDXGXIGNLXXY
GHEKPPLINVXXXPPTXXIALXSGTKDELADPXXXKXLXSLLPPETKXXWDXIEXXAXLD
FXWA

Frame A:
lfi*ililklkyeiklfnfyiiifnnklfiick*ykhf*ryqynl*sfkg*hkrdyc*cn
ynsescrily*yse*qwipm*asfsynrrwlyigci*nsifl*q*sks***n*ttnsiat
wfirlidnmds**cksittihiirygircmdg**pvvihlvsiildwm*RVENFGNSHSM
IWGWYDLPSMVDYI---

---mvxwxxxrxlqsixxaickwx*twxxfxxkxgpfxxixkfktistl*lxxywxfxtl
ws*ktpxn*cxkxtpxxkxcxixwdkg*igrpxxxkaixixltx*nxtxlgxn*kxxxfr
fxlg

Frame B:
ysykf*y*n*nmklnylisillfliinyssfvssinifkdiniiydhlkddikeiivdat
ttvnhveyfidivnsngypcehhsvitedgyilgvfripysynnnqnlnnktrqpillqh
glldssitwivnnanqslpfilsdmgydvwmgnnpw*yi*yqsy*igcke*rilgilir*
ygvgtiyhqwliis---

---WLXGPXKGAFNQSXXPFVSGNEPGGTSLXXMXXFXQLXNSKXFQXYDXGXIGNLXXY
GHEKPPLINVXXXPPTXXIALXSGTKDELADPXXXKXLXSLLPPETKXXWDXIEXXAXLD
FXWA

Frame C:
ihinfnikiki*n*ii*flyyyf***iihhl*vv*tflkisi*smii*rmt*krlllmql
qq*im*ntlli**iamdthvsiiql*qkmviywvylefhilitiikiliikldnqfycnm
vy*thr*hg*limqinhyhsyyqiwdtmygwvitrgntfsinhtrldvksrefwefsfdd
mglvrsting*ly---

---gyxxxxkxpsinqxxhl*vxmnlxxlxlxxwxxsxn*xiqnxfnxmixexlxixyxm
vmknpx*lmxkxxpxlxxlxyxlgqrmnwqtxxmxsx*xxsyxlkxnxlgtxlkxxxx*i
lxgx

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK789 (AFK789Q) /CSM/AF/AFK7-D/AFK789Q.Seq.d/                       1326   0.0
VFK724 (VFK724Q) /CSM/VF/VFK7-A/VFK724Q.Seq.d/                        954   0.0
SSF723 (SSF723Q) /CSM/SS/SSF7-A/SSF723Q.Seq.d/                        954   0.0
SFK691 (SFK691Q) /CSM/SF/SFK6-D/SFK691Q.Seq.d/                        920   0.0
SFE241 (SFE241Q) /CSM/SF/SFE2-B/SFE241Q.Seq.d/                        749   0.0
CHL683 (CHL683Q) /CSM/CH/CHL6-D/CHL683Q.Seq.d/                        712   0.0
SSL833 (SSL833Q) /CSM/SS/SSL8-B/SSL833Q.Seq.d/                         38   0.24
SSG214 (SSG214Q) /CSM/SS/SSG2-A/SSG214Q.Seq.d/                         38   0.24
SSE289 (SSE289Q) /CSM/SS/SSE2-D/SSE289Q.Seq.d/                         38   0.24
SSE210 (SSE210Q) /CSM/SS/SSE2-A/SSE210Q.Seq.d/                         38   0.24

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.   749   0.0    9
BX106575|BX106575.1 RZPD Homo sapiens cDNA clone IMAGp998G0283 = IMAGE:109849 5' EST.    38   0.055  2
AE014836|AE014836.1 Plasmodium falciparum 3D7 chromosome 11 section 1 of 8 of the complete sequence.    48   0.26   1
CD494861|CD494861.1 CDA13-B09.y1d-s SHGC-CDA Gasterosteus aculeatus cDNA clone CDA13-B09 3', mRNA sequence.    48   0.26   1
AL929351|AL929351.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 1/4.    32   0.52   2
AX347156|AX347156.1 Sequence 2227 from Patent WO0200928.           40   0.78   3
AP005208|AP005208.3 Homo sapiens genomic DNA, chromosome 18 clone:RP11-138C24, complete sequence.    36   0.87   6
AL022140|AL022140.1 Arabidopsis thaliana DNA chromosome 4, BAC clone F1N20 (ESSA  project).    40   1.3    2
AI546753|AI546753.1 BSBmL3SZ12D15SK Brugia malayi infective larva cDNA (SAW94WL-BmL3) Brugia malayi cDNA clone BSBmL3SZ12D15SK 5', mRNA sequence.    38   2.1    2
AC135363|AC135363.2 Mus musculus chromosome UNK clone RP23-341B24, WORKING DRAFT SEQUENCE, 18 unordered pieces.    42   2.5    4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE014297_4041(AE014297|pid:none) Drosophila melanogaster chromos...    97   3e-22
(Q5W064) RecName: Full=Lipase member J;          EC=3.1.1.-; Alt...    89   3e-22
AE014134_2003(AE014134|pid:none) Drosophila melanogaster chromos...    87   5e-22
BC055815_1(BC055815|pid:none) Mus musculus lipase, family member...    87   2e-21
(Q8BM14) RecName: Full=Lipase member K;          EC=3.1.1.-; Alt...    86   3e-21
(Q67ZU1) RecName: Full=Triacylglycerol lipase 2;          EC=3.1...    92   8e-20
AK175707_1(AK175707|pid:none) Arabidopsis thaliana mRNA, partial...    92   8e-20
(Q9Z0M5) RecName: Full=Lysosomal acid lipase/cholesteryl ester h...    81   8e-20
(P04634) RecName: Full=Gastric triacylglycerol lipase;          ...    88   1e-19
AE014296_3568(AE014296|pid:none) Drosophila melanogaster chromos...    89   2e-19

 psg: 0.85  gvh: 0.40  alm: 0.50  top: 0.37  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vesicles of secretory system
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal

>> prediction for AFK789 is cyt

>AFK789F.Seq
TTATTCATATAAATTTTAATATTAAAATTAAAATATGAAATTAAATTATTTAATTTCTAT
ATTATTATTTTTAATAATAAATTATTCATCATTTGTAAGTAGTATAAACATTTTTAAAGA
TATCAATATAATCTATGATCATTTAAAGGATGACATAAAAGAGATTATTGTTGATGCAAC
TACAACAGTGAATCATGTAGAATACTTTATTGATATAGTGAATAGCAATGGATACCCATG
TGAGCATCATTCAGTTATAACAGAAGATGGTTATATATTGGGTGTATTTAGAATTCCATA
TTCTTATAACAATAATCAAAATCTTAATAATAAAACTAGACAACCAATTCTATTGCAACA
TGGTTTATTAGACTCATCGATAACATGGATAGTTAATAATGCAAATCAATCACTACCATT
CATATTATCAGATATGGGATACGATGTATGGATGGGTAATAACCCGTGGTAATACATTTA
GTATCAATCATACTAGATTGGATGTAAAGAGTAGAGAATTTTGGGAATTCTCATTCGATG
ATATGGGGTTGGTACGATCTACCATCAATGGTTGATTATATCA----------

>AFK789Z.Seq
----------ATGGTTATNTGGNCCNCANAAAGGNGCCTTCAATCAATCAANAANGCCAT
TTGTAAGTGGNAATGAACCTGGNGGNACTTCNCTTANAAANATGGNCCNTTTCNCNCAAT
TAGNAAATTCAAAACNATTTCAACNTTATGATTANGGAGNTATTGGNAATTTANTACNTT
ATGGTCATGAAAAACCCCCNTTAATTAATGTTNAAAANANACCCCCNACTGNAAANATTG
CNTTATTNTCTGGGACAAAGGATGAATTGGCAGACCCNANANATGNAAAGCNATTAGNAT
CNCTCTTACCNCCTGAAACNAAACNCNCTTGGGACANAATTGAAAANTANGCNCNTTTAG
ATTTTGNTTGGGCNA

>AFK789P.Seq
TTATTCATATAAATTTTAATATTAAAATTAAAATATGAAATTAAATTATTTAATTTCTAT
ATTATTATTTTTAATAATAAATTATTCATCATTTGTAAGTAGTATAAACATTTTTAAAGA
TATCAATATAATCTATGATCATTTAAAGGATGACATAAAAGAGATTATTGTTGATGCAAC
TACAACAGTGAATCATGTAGAATACTTTATTGATATAGTGAATAGCAATGGATACCCATG
TGAGCATCATTCAGTTATAACAGAAGATGGTTATATATTGGGTGTATTTAGAATTCCATA
TTCTTATAACAATAATCAAAATCTTAATAATAAAACTAGACAACCAATTCTATTGCAACA
TGGTTTATTAGACTCATCGATAACATGGATAGTTAATAATGCAAATCAATCACTACCATT
CATATTATCAGATATGGGATACGATGTATGGATGGGTAATAACCCGTGGTAATACATTTA
GTATCAATCATACTAGATTGGATGTAAAGAGTAGAGAATTTTGGGAATTCTCATTCGATG
ATATGGGGTTGGTACGATCTACCATCAATGGTTGATTATATCA----------ATGGTTA
TNTGGNCCNCANAAAGGNGCCTTCAATCAATCAANAANGCCATTTGTAAGTGGNAATGAA
CCTGGNGGNACTTCNCTTANAAANATGGNCCNTTTCNCNCAATTAGNAAATTCAAAACNA
TTTCAACNTTATGATTANGGAGNTATTGGNAATTTANTACNTTATGGTCATGAAAAACCC
CCNTTAATTAATGTTNAAAANANACCCCCNACTGNAAANATTGCNTTATTNTCTGGGACA
AAGGATGAATTGGCAGACCCNANANATGNAAAGCNATTAGNATCNCTCTTACCNCCTGAA
ACNAAACNCNCTTGGGACANAATTGAAAANTANGCNCNTTTAGATTTTGNTTGGGCNA