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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK808
Library AF
(Link to library)
Clone ID AFK808
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14931-1
Original site URL
Representative seq. ID AFK808P
(Link to Original site)
Representative DNA sequence 
>AFK808 (AFK808Q) /CSM/AF/AFK8-A/AFK808Q.Seq.d/
AAATGTCTTCAAATAAAATTGGTAATAAATTAAGTCTTGACAAGGTAGATGTAAAAGGTA
AAAGAGTTTTAATTAGAGTTGATTATAATGTACCATTAGATAAAAATAATAATATTACAA
GTACTCAAAGAATTGATGCATCAATTCCAACATTAGAATATTGTTTAAAGAATGGTGCTA
AATCAATTGTATTGATGTCACATTTAGGTCGTCCAGATGGTTTGGTTAAACCAGAGTATA
GTTTGAAACCAGTGGTTAAAGTATTGGAAGATCAATTAAAAAGACCAATTAAATTCTTAT
CAGATTGTGTTGGTGAACAAGTTGAAAAAGAATGTGCAAATCCAGAGGAAGGTACCGTTA
TTTTATTGGAGAATCTCCGTTTCCACATTGAAGAGGAAGGTTCAGGTGTTGATGCAGAGG
GTAAAAAGGTCAAAGCCAACCCAGAGAAAGTTAAAGAATTCCGTGAATCACTCACCAAGC
TCGGTGATGTCTATGTGAACGATGCCTTTGGTACTGCTCATAGAGCACATAGTTCAATGG
TTXXXXXXXXXXCTTGGCAATTCTTGGCGGTGCAAAGGTTAGTGATAAAATTAAACTCAT
TGAAAACCTTTTGTATAAAGTCGATGAAATGATCATTGGTGGTGGTATGGCCTTCACTTT
CAAGAAATTCATTGATAACAAAGAGATTGGTTCATCATTGTTTGAGAAAACAGCCGAACA
AATCACCAAAGATATCATTGCCAAAGCCGCTAAAAATAATGTAAAATTACATTTCCCAGT
CGATTATGTCATTGCCGATAAATTTGATAATGATGCCAACATCAAAACCGTCACTCAAGA
TCAAGGTATCCCAGAAGGTTGGATGGGTTTAGATTGTGGTCCAGAGACTATCAAAGAAAA
CAGAGATACCATCTCAAGAGCAAAAACCATCGTTTGGAATGGTCCTATGGGTGTATTTGA
AAAGAGCAATTTCGAAGCCGGTACAAAAGCTGCCATGGATGATGTTGTCAATGCCACAAC
CAATGGTGCTATCACTATCATTGGTGGTGGTGATACCGCCACTTGTGCTGCCAAATACAA
CACTGAAGATAAAGTTAGTCATGTCTCAACTGGTGGTGGTGCTTCATTAGAATTATTAGA
AGGTAAAGAATTACCAGGTGTTACTGCTTTATCNATTTATAATTTTAAAAAATAATTATA
AAAAAATAATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
MSSNKIGNKLSLDKVDVKGKRVLIRVDYNVPLDKNNNITSTQRIDASIPTLEYCLKNGAK
SIVLMSHLGRPDGLVKPEYSLKPVVKVLEDQLKRPIKFLSDCVGEQVEKECANPEEGTVI
LLENLRFHIEEEGSGVDAEGKKVKANPEKVKEFRESLTKLGDVYVNDAFGTAHRAHSSMV
---

---LAILGGAKVSDKIKLIENLLYKVDEMIIGGGMAFTFKKFIDNKEIGSSLFEKTAEQI
TKDIIAKAAKNNVKLHFPVDYVIADKFDNDANIKTVTQDQGIPEGWMGLDCGPETIKENR
DTISRAKTIVWNGPMGVFEKSNFEAGTKAAMDDVVNATTNGAITIIGGGDTATCAAKYNT
EDKVSHVSTGGGASLELLEGKELPGVTALSIYNFKK*l*knn
Translated Amino Acid sequence (All Frames) 
Frame A:
kclqiklvin*vltr*m*kvkef*leliimyh*ikiiilqvlkelmhqfqh*niv*rmvl
nqly*chi*vvqmvwlnqsiv*nqwlkywkin*kdqlnsyqivlvnklkknvqiqrkvpl
fywrisvstlkrkvqvlmqrvkrskptqrklknsvnhspssvmsm*tmplvlliehivqw
---

---lgnswrckg***n*th*kpfv*sr*ndhwwwyglhfqeih**qrdwfiiv*ensrtn
hqryhcqsr*k*ckitfpsrlchcr*i***cqhqnrhsrsryprrldgfrlwsrdyqrkq
ryhlksknhrlewsygci*keqfrsrykschg*ccqchnqwcyhyhwww*yrhlccqiqh
*r*s*sclnwwwcfiriirr*ritrcycfixl*f*kiiikk*

Frame B:
nvfk*nw**iks*qgrckr*ksfn*s*l*ctir*k**yykyskn*cinsnirilfkewc*
incidvtfrssrwfg*trv*fetsg*sigrsikktn*ilirlcw*ts*krmcksrgryry
figespfph*rgrfrc*crg*kgqsqpres*rip*ithqar*clcerclwycs*st*fng
---

---LAILGGAKVSDKIKLIENLLYKVDEMIIGGGMAFTFKKFIDNKEIGSSLFEKTAEQI
TKDIIAKAAKNNVKLHFPVDYVIADKFDNDANIKTVTQDQGIPEGWMGLDCGPETIKENR
DTISRAKTIVWNGPMGVFEKSNFEAGTKAAMDDVVNATTNGAITIIGGGDTATCAAKYNT
EDKVSHVSTGGGASLELLEGKELPGVTALSIYNFKK*l*knn

Frame C:
MSSNKIGNKLSLDKVDVKGKRVLIRVDYNVPLDKNNNITSTQRIDASIPTLEYCLKNGAK
SIVLMSHLGRPDGLVKPEYSLKPVVKVLEDQLKRPIKFLSDCVGEQVEKECANPEEGTVI
LLENLRFHIEEEGSGVDAEGKKVKANPEKVKEFRESLTKLGDVYVNDAFGTAHRAHSSMV
---

---wqflavqrlviklnslktfciksmk*slvvvwpslsrnslitkrlvhhclrkqpnks
pkislpkplkim*nyisqsimslpinlimmptskpslkikvsqkvgwv*ivvqrlskkte
ipsqeqkpsfgmvlwvylkraiskpvqklpwmmlsmpqpmvlslslvvvipplvlpnttl
kiklvmsqlvvvlh*ny*kvknyqvlllyxfiilknnykkii
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK808 (AFK808Q) /CSM/AF/AFK8-A/AFK808Q.Seq.d/                       2311   0.0
AFM289 (AFM289Q) /CSM/AF/AFM2-D/AFM289Q.Seq.d/                       1271   0.0
VFL513 (VFL513Q) /CSM/VF/VFL5-A/VFL513Q.Seq.d/                       1269   0.0
VFI829 (VFI829Q) /CSM/VF/VFI8-B/VFI829Q.Seq.d/                       1269   0.0
VFI421 (VFI421Q) /CSM/VF/VFI4-A/VFI421Q.Seq.d/                       1269   0.0
CFC187 (CFC187Q) /CSM/CF/CFC1-D/CFC187Q.Seq.d/                       1269   0.0
AFN807 (AFN807Q) /CSM/AF/AFN8-A/AFN807Q.Seq.d/                       1269   0.0
AFM292 (AFM292Q) /CSM/AF/AFM2-D/AFM292Q.Seq.d/                       1269   0.0
VFF407 (VFF407Q) /CSM/VF/VFF4-A/VFF407Q.Seq.d/                       1267   0.0
VHD789 (VHD789Q) /CSM/VH/VHD7-D/VHD789Q.Seq.d/                       1263   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF316577|AF316577.2 Dictyostelium discoideum phosphoglycerate kinase (pgkA) mRNA, complete cds.  1231   0.0    2
AF018637|AF018637.1 Dictyostelium discoideum phosphoglycerate kinase (pgkA) mRNA, partial cds.  1231   0.0    2
AX488918|AX488918.1 Sequence 6218 from Patent WO02053728.          82   2e-43  6
U25180|U25180.1 Candida albicans phosphoglycerate kinase (PGK1) gene, complete cds.    82   2e-42  6
D12474|D12474.1 Candida maltosa C-PGK1 gene for phosphoglycerate kinase, complete cds.    96   3e-40  6
L36833|L36833.1 Schistosoma mansoni (clone C5-1B3) phosphoglycerate kinase (PGK) gene, complete cds.    50   2e-29  7
J01342|J01342.1 S.cerevisiae 3-phosphoglycerate kinase (pgk) gene.    78   6e-27  5
X17654|X17654.1 Kluyveromyces lactis pgk gene for 3-phosphoglycerate kinase (EC 2.7.2.3).    62   3e-25  6
AX536476|AX536476.1 Sequence 77 from Patent WO02064766.            78   5e-24  4
AL403633|AL403633.1 T7 end of clone AT0AA009H08 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    94   2e-22  2
dna update 2004. 8. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9GPM4) RecName: Full=Phosphoglycerate kinase;          EC=2.7....   365   0.0
AB172722_1(AB172722|pid:none) Macaca fascicularis brain cDNA clo...   244   e-129
AY888092_1(AY888092|pid:none) Synthetic construct Homo sapiens c...   244   e-129
(A5A6K4) RecName: Full=Phosphoglycerate kinase 1;          EC=2....   244   e-129
(Q5NVB5) RecName: Full=Phosphoglycerate kinase 1;          EC=2....   244   e-129
CR456716_1(CR456716|pid:none) Homo sapiens full open reading fra...   244   e-129
CR926123_1(CR926123|pid:none) Pongo abelii mRNA; cDNA DKFZp459A2...   244   e-129
(Q7SIB7) RecName: Full=Phosphoglycerate kinase 1;          EC=2....   246   e-129
FJ236704_1(FJ236704|pid:none) Ovis aries phosphoglycerate kinase...   243   e-128
AB170091_1(AB170091|pid:none) Macaca fascicularis brain cDNA clo...   243   e-128
protein update 2009. 4.25
PSORT 

 psg: 0.59  gvh: 0.36  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for AFK808 is cyt
5' end seq. ID AFK808F
5' end seq. 
>AFK808F.Seq
AAATGTCTTCAAATAAAATTGGTAATAAATTAAGTCTTGACAAGGTAGATGTAAAAGGTA
AAAGAGTTTTAATTAGAGTTGATTATAATGTACCATTAGATAAAAATAATAATATTACAA
GTACTCAAAGAATTGATGCATCAATTCCAACATTAGAATATTGTTTAAAGAATGGTGCTA
AATCAATTGTATTGATGTCACATTTAGGTCGTCCAGATGGTTTGGTTAAACCAGAGTATA
GTTTGAAACCAGTGGTTAAAGTATTGGAAGATCAATTAAAAAGACCAATTAAATTCTTAT
CAGATTGTGTTGGTGAACAAGTTGAAAAAGAATGTGCAAATCCAGAGGAAGGTACCGTTA
TTTTATTGGAGAATCTCCGTTTCCACATTGAAGAGGAAGGTTCAGGTGTTGATGCAGAGG
GTAAAAAGGTCAAAGCCAACCCAGAGAAAGTTAAAGAATTCCGTGAATCACTCACCAAGC
TCGGTGATGTCTATGTGAACGATGCCTTTGGTACTGCTCATAGAGCACATAGTTCAATGG
TT----------
Length of 5' end seq. 542
3' end seq. ID AFK808Z
3' end seq. 
>AFK808Z.Seq
----------CTTGGCAATTCTTGGCGGTGCAAAGGTTAGTGATAAAATTAAACTCATTG
AAAACCTTTTGTATAAAGTCGATGAAATGATCATTGGTGGTGGTATGGCCTTCACTTTCA
AGAAATTCATTGATAACAAAGAGATTGGTTCATCATTGTTTGAGAAAACAGCCGAACAAA
TCACCAAAGATATCATTGCCAAAGCCGCTAAAAATAATGTAAAATTACATTTCCCAGTCG
ATTATGTCATTGCCGATAAATTTGATAATGATGCCAACATCAAAACCGTCACTCAAGATC
AAGGTATCCCAGAAGGTTGGATGGGTTTAGATTGTGGTCCAGAGACTATCAAAGAAAACA
GAGATACCATCTCAAGAGCAAAAACCATCGTTTGGAATGGTCCTATGGGTGTATTTGAAA
AGAGCAATTTCGAAGCCGGTACAAAAGCTGCCATGGATGATGTTGTCAATGCCACAACCA
ATGGTGCTATCACTATCATTGGTGGTGGTGATACCGCCACTTGTGCTGCCAAATACAACA
CTGAAGATAAAGTTAGTCATGTCTCAACTGGTGGTGGTGCTTCATTAGAATTATTAGAAG
GTAAAGAATTACCAGGTGTTACTGCTTTATCNATTTATAATTTTAAAAAATAATTATAAA
AAAATAATA
Length of 3' end seq. 659
Connected seq. ID AFK808P
Connected seq. 
>AFK808P.Seq
AAATGTCTTCAAATAAAATTGGTAATAAATTAAGTCTTGACAAGGTAGATGTAAAAGGTA
AAAGAGTTTTAATTAGAGTTGATTATAATGTACCATTAGATAAAAATAATAATATTACAA
GTACTCAAAGAATTGATGCATCAATTCCAACATTAGAATATTGTTTAAAGAATGGTGCTA
AATCAATTGTATTGATGTCACATTTAGGTCGTCCAGATGGTTTGGTTAAACCAGAGTATA
GTTTGAAACCAGTGGTTAAAGTATTGGAAGATCAATTAAAAAGACCAATTAAATTCTTAT
CAGATTGTGTTGGTGAACAAGTTGAAAAAGAATGTGCAAATCCAGAGGAAGGTACCGTTA
TTTTATTGGAGAATCTCCGTTTCCACATTGAAGAGGAAGGTTCAGGTGTTGATGCAGAGG
GTAAAAAGGTCAAAGCCAACCCAGAGAAAGTTAAAGAATTCCGTGAATCACTCACCAAGC
TCGGTGATGTCTATGTGAACGATGCCTTTGGTACTGCTCATAGAGCACATAGTTCAATGG
TT----------CTTGGCAATTCTTGGCGGTGCAAAGGTTAGTGATAAAATTAAACTCAT
TGAAAACCTTTTGTATAAAGTCGATGAAATGATCATTGGTGGTGGTATGGCCTTCACTTT
CAAGAAATTCATTGATAACAAAGAGATTGGTTCATCATTGTTTGAGAAAACAGCCGAACA
AATCACCAAAGATATCATTGCCAAAGCCGCTAAAAATAATGTAAAATTACATTTCCCAGT
CGATTATGTCATTGCCGATAAATTTGATAATGATGCCAACATCAAAACCGTCACTCAAGA
TCAAGGTATCCCAGAAGGTTGGATGGGTTTAGATTGTGGTCCAGAGACTATCAAAGAAAA
CAGAGATACCATCTCAAGAGCAAAAACCATCGTTTGGAATGGTCCTATGGGTGTATTTGA
AAAGAGCAATTTCGAAGCCGGTACAAAAGCTGCCATGGATGATGTTGTCAATGCCACAAC
CAATGGTGCTATCACTATCATTGGTGGTGGTGATACCGCCACTTGTGCTGCCAAATACAA
CACTGAAGATAAAGTTAGTCATGTCTCAACTGGTGGTGGTGCTTCATTAGAATTATTAGA
AGGTAAAGAATTACCAGGTGTTACTGCTTTATCNATTTATAATTTTAAAAAATAATTATA
AAAAAATAATA
Length of connected seq. 1201
Full length Seq ID -
Full length Seq. -
Length of full length seq. -