>AFK815 (AFK815Q) /CSM/AF/AFK8-A/AFK815Q.Seq.d/
AGTTAATTTTCATTTGTAAATATAAAAAAAAAATGGAATCATTAAAAAACAATGGTGAAA
AAATTGAAAATTTTTTAAATCAATTAAAAAATCTTCAATCAAATGTAGACAAAGATTTTA
AAGAATTTTCAAATTCTCCAAATACTTTAAATGCTGAAAAAGATGGAGAGTTTCTTGATA
AACATGATGAAGAATTATACAACAAACAAATAATTCGTTCAAGACTAGAAGATAAAATTT
CAAATTTAAGTTCTTCTATGATTAATGATATTGGGAATGATTTTCAAACTGGTTTTCAAA
CAATTCTATACAATTTACCAGGTGTTGGTCCAGTTTTCCAAATGATCTTTAATATTATGG
GTAATGGTATAAATTTAGAAAAATTTTACCAAGAAATCATTACACAAGTAGAACAAATGA
TTAAAAAATCATTAGAAGATTATTATAAAAACCAATGTAATACAATTTTTAAAAATTTAG
GAAAAGCATGTGATCAACATAAAGATCTAACTCAAAAATGGTATGATAAAAATGGTATCA
AATCAATGAACCATTTXXXXXXXXXXTCAAGAAGGTTATTGGATAACATATCCACCACCC
AATTTATAAGGTTTGGGTTCCACCACAACAAGCATCGTGGGTATCTCCTCCTGCCTCGGA
ATTGGCACATAAATTCTTTTTGGCCCATTCTGACAATTATCCTCAAGGTGGTGATCTAAT
TTATATTAAAAAAGATGGAGTTTATGAACTTAGTCCAAATAAAATAACTCATGCCCTTGA
AGTTTATTATAGCTCTGGGGGTGGATATGGTGTTAATGACTATCCAAGTTTTCGTTATGG
TGGAAAACCAATCATACCTCTTCTCGCTCCAGTTGGTAATGCTGTGTTTACAATGTTAAA
TCCAAATCGTTATAAAAGAACCTTTAAAATTAGAATTGTTCATGTCATTGTTAGAGAAGC
CAAATGGAATTTAGATTGCTATGATAATCAAGTTGGGGAAGCAGGTTCATTTAACCAAAA
TTTTGTTTTCACTTCAACTAATATAGTTTCTGACCCAATTTGTGGGAATATTGGAACTGT
TGGTATCACTGAAATTCCAGGACCATTTACAACTGATAAAAAGTATATTAGATTAACATG
CATTCGTAAAGTAAATTTCCCAGCATCAAAAAATGAAGGATATGCCGTTGGATCTCGTAT
TTTATCAGTACAATTAATTATTTATAACCACTTCAATAAAATAATTTTCATTTAAAATAT
TG

LIFICKYKKKMESLKNNGEKIENFLNQLKNLQSNVDKDFKEFSNSPNTLNAEKDGEFLDK
HDEELYNKQIIRSRLEDKISNLSSSMINDIGNDFQTGFQTILYNLPGVGPVFQMIFNIMG
NGINLEKFYQEIITQVEQMIKKSLEDYYKNQCNTIFKNLGKACDQHKDLTQKWYDKNGIK
SMNH---

---SRRLLDNISTTQFIRFGFHHNKHRGYLLLPRNWHINSFWPILTIILKVVI*filkkm
efmnlvqik*lmplkfiialgvdmvlmtiqvfvmvenqsylfslqlvmlclqc*iqivik
eplklelfmsllekpngi*iamiiklgkqvhltkilfslqli*fltqfvgilellvslkf
qdhlqliksild*hafvk*isqhqkmkdmpldlvfyqyn*lfittsik*fsfkil

Frame A:
s*fsfvnikkkwnh*ktmvkklkif*in*kifnqm*tkilknfqilqil*mlkkmesfli
nmmknyttnk*fvqd*kikfqi*vll*lmilgmifklvfkqfytiyqvlvqfsk*slilw
vmv*i*knftkkslhk*nk*lknh*kiiiktnviqflki*ekhviniki*lkngmikmvs
nq*ti---

---SRRLLDNISTTQFIRFGFHHNKHRGYLLLPRNWHINSFWPILTIILKVVI*filkkm
efmnlvqik*lmplkfiialgvdmvlmtiqvfvmvenqsylfslqlvmlclqc*iqivik
eplklelfmsllekpngi*iamiiklgkqvhltkilfslqli*fltqfvgilellvslkf
qdhlqliksild*hafvk*isqhqkmkdmpldlvfyqyn*lfittsik*fsfkil

Frame B:
vnfhl*i*kkngiikkqw*kn*kffksikkssikcrqrf*rifkfskyfkc*krwrvs**
t**riiqqtnnsfktrr*nfkfkffyd**ywe*fsnwfsnnsiqftrcwssfpndl*yyg
*wykfrkilprnhytsrtnd*kiirrll*kpm*ynf*kfrksm*st*rsnskmv**kwyq
inepf---

---qegywitypppnl*glgstttsivgissclgigt*ilfgpf*qlssrw*snly*krw
sl*t*sk*nnscp*sll*lwgwiwc**lskfslwwktnhtssrssw*ccvynvksksl*k
nl*n*ncschc*rsqmefrll**sswgsrfi*pkfcfhfn*ysf*pnlweywncwyh*ns
rtiyn**kvy*inmhs*skfpsikk*ricrwisyfistinyl*plq*nnfhlky

Frame C:
LIFICKYKKKMESLKNNGEKIENFLNQLKNLQSNVDKDFKEFSNSPNTLNAEKDGEFLDK
HDEELYNKQIIRSRLEDKISNLSSSMINDIGNDFQTGFQTILYNLPGVGPVFQMIFNIMG
NGINLEKFYQEIITQVEQMIKKSLEDYYKNQCNTIFKNLGKACDQHKDLTQKWYDKNGIK
SMNH---

---kkvig*hihhpiykvwvppqqaswvsppaselahkfflahsdnypqggdliyikkdg
vyelspnkithalevyyssgggygvndypsfryggkpiipllapvgnavftmlnpnrykr
tfkirivhvivreakwnldcydnqvgeagsfnqnfvftstnivsdpicgnigtvgiteip
gpfttdkkyirltcirkvnfpasknegyavgsrilsvqliiynhfnkiifi*ni

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK815 (AFK815Q) /CSM/AF/AFK8-A/AFK815Q.Seq.d/                       2260   0.0
AFH818 (AFH818Q) /CSM/AF/AFH8-A/AFH818Q.Seq.d/                       2228   0.0
AFM155 (AFM155Q) /CSM/AF/AFM1-C/AFM155Q.Seq.d/                       1366   0.0
AFO242 (AFO242Q) /CSM/AF/AFO2-B/AFO242Q.Seq.d/                       1360   0.0
VFI666 (VFI666Q) /CSM/VF/VFI6-C/VFI666Q.Seq.d/                       1358   0.0
CHD481 (CHD481Q) /CSM/CH/CHD4-D/CHD481Q.Seq.d/                       1358   0.0
AFK522 (AFK522Q) /CSM/AF/AFK5-A/AFK522Q.Seq.d/                       1358   0.0
AFE435 (AFE435Q) /CSM/AF/AFE4-B/AFE435Q.Seq.d/                       1358   0.0
AFO392 (AFO392Q) /CSM/AF/AFO3-D/AFO392Q.Seq.d/                       1350   0.0
AFN550 (AFN550Q) /CSM/AF/AFN5-C/AFN550Q.Seq.d/                       1346   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117072|AC117072.3 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.  1211   0.0    14
M15968|M15968.1 D.discoideum M3R gene, exons 1-3.                 573   0.0    3
M15967|M15967.1 D.discoideum M3L gene, exons 1-3.                 238   e-150  3
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    38   1e-10  23
AB063521|AB063521.2 Wigglesworthia brevipalpis DNA, complete genome, section 1/2.    34   2e-09  21
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    34   2e-09  20
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          34   5e-08  30
BX842644|BX842644.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 3/4.    34   8e-08  20
AF303741|AF303741.1 Chilo iridescent virus complete genome.        32   4e-07  17
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    34   7e-07  24

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P11872) RecName: Full=cAMP-regulated M3R protein; AltName: Full...   407   e-115
AC117072_17(AC117072|pid:none) Dictyostelium discoideum chromoso...   281   4e-75
AE014187_536(AE014187|pid:none) Plasmodium falciparum 3D7 chromo...    48   8e-04
AE014186_384(AE014186|pid:none) Plasmodium falciparum 3D7 chromo...    45   0.007
CR380952_40(CR380952|pid:none) Candida glabrata strain CBS138 ch...    43   0.020
EF158087_1(EF158087|pid:none) Plasmodium falciparum erythrocyte ...    41   0.075
AC116920_30(AC116920|pid:none) Dictyostelium discoideum chromoso...    40   0.13
CP000879_149(CP000879|pid:none) Petrotoga mobilis SJ95, complete...    40   0.17
(O51267) RecName: Full=Leucyl-tRNA synthetase;          EC=6.1.1...    40   0.22
CP001205_240(CP001205|pid:none) Borrelia burgdorferi ZS7, comple...    40   0.22

 psg: 0.56  gvh: 0.29  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.13  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum

>> prediction for AFK815 is nuc

>AFK815F.Seq
AGTTAATTTTCATTTGTAAATATAAAAAAAAAATGGAATCATTAAAAAACAATGGTGAAA
AAATTGAAAATTTTTTAAATCAATTAAAAAATCTTCAATCAAATGTAGACAAAGATTTTA
AAGAATTTTCAAATTCTCCAAATACTTTAAATGCTGAAAAAGATGGAGAGTTTCTTGATA
AACATGATGAAGAATTATACAACAAACAAATAATTCGTTCAAGACTAGAAGATAAAATTT
CAAATTTAAGTTCTTCTATGATTAATGATATTGGGAATGATTTTCAAACTGGTTTTCAAA
CAATTCTATACAATTTACCAGGTGTTGGTCCAGTTTTCCAAATGATCTTTAATATTATGG
GTAATGGTATAAATTTAGAAAAATTTTACCAAGAAATCATTACACAAGTAGAACAAATGA
TTAAAAAATCATTAGAAGATTATTATAAAAACCAATGTAATACAATTTTTAAAAATTTAG
GAAAAGCATGTGATCAACATAAAGATCTAACTCAAAAATGGTATGATAAAAATGGTATCA
AATCAATGAACCATTT----------

>AFK815Z.Seq
----------TCAAGAAGGTTATTGGATAACATATCCACCACCCAATTTATAAGGTTTGG
GTTCCACCACAACAAGCATCGTGGGTATCTCCTCCTGCCTCGGAATTGGCACATAAATTC
TTTTTGGCCCATTCTGACAATTATCCTCAAGGTGGTGATCTAATTTATATTAAAAAAGAT
GGAGTTTATGAACTTAGTCCAAATAAAATAACTCATGCCCTTGAAGTTTATTATAGCTCT
GGGGGTGGATATGGTGTTAATGACTATCCAAGTTTTCGTTATGGTGGAAAACCAATCATA
CCTCTTCTCGCTCCAGTTGGTAATGCTGTGTTTACAATGTTAAATCCAAATCGTTATAAA
AGAACCTTTAAAATTAGAATTGTTCATGTCATTGTTAGAGAAGCCAAATGGAATTTAGAT
TGCTATGATAATCAAGTTGGGGAAGCAGGTTCATTTAACCAAAATTTTGTTTTCACTTCA
ACTAATATAGTTTCTGACCCAATTTGTGGGAATATTGGAACTGTTGGTATCACTGAAATT
CCAGGACCATTTACAACTGATAAAAAGTATATTAGATTAACATGCATTCGTAAAGTAAAT
TTCCCAGCATCAAAAAATGAAGGATATGCCGTTGGATCTCGTATTTTATCAGTACAATTA
ATTATTTATAACCACTTCAATAAAATAATTTTCATTTAAAATATTG

>AFK815P.Seq
AGTTAATTTTCATTTGTAAATATAAAAAAAAAATGGAATCATTAAAAAACAATGGTGAAA
AAATTGAAAATTTTTTAAATCAATTAAAAAATCTTCAATCAAATGTAGACAAAGATTTTA
AAGAATTTTCAAATTCTCCAAATACTTTAAATGCTGAAAAAGATGGAGAGTTTCTTGATA
AACATGATGAAGAATTATACAACAAACAAATAATTCGTTCAAGACTAGAAGATAAAATTT
CAAATTTAAGTTCTTCTATGATTAATGATATTGGGAATGATTTTCAAACTGGTTTTCAAA
CAATTCTATACAATTTACCAGGTGTTGGTCCAGTTTTCCAAATGATCTTTAATATTATGG
GTAATGGTATAAATTTAGAAAAATTTTACCAAGAAATCATTACACAAGTAGAACAAATGA
TTAAAAAATCATTAGAAGATTATTATAAAAACCAATGTAATACAATTTTTAAAAATTTAG
GAAAAGCATGTGATCAACATAAAGATCTAACTCAAAAATGGTATGATAAAAATGGTATCA
AATCAATGAACCATTT----------TCAAGAAGGTTATTGGATAACATATCCACCACCC
AATTTATAAGGTTTGGGTTCCACCACAACAAGCATCGTGGGTATCTCCTCCTGCCTCGGA
ATTGGCACATAAATTCTTTTTGGCCCATTCTGACAATTATCCTCAAGGTGGTGATCTAAT
TTATATTAAAAAAGATGGAGTTTATGAACTTAGTCCAAATAAAATAACTCATGCCCTTGA
AGTTTATTATAGCTCTGGGGGTGGATATGGTGTTAATGACTATCCAAGTTTTCGTTATGG
TGGAAAACCAATCATACCTCTTCTCGCTCCAGTTGGTAATGCTGTGTTTACAATGTTAAA
TCCAAATCGTTATAAAAGAACCTTTAAAATTAGAATTGTTCATGTCATTGTTAGAGAAGC
CAAATGGAATTTAGATTGCTATGATAATCAAGTTGGGGAAGCAGGTTCATTTAACCAAAA
TTTTGTTTTCACTTCAACTAATATAGTTTCTGACCCAATTTGTGGGAATATTGGAACTGT
TGGTATCACTGAAATTCCAGGACCATTTACAACTGATAAAAAGTATATTAGATTAACATG
CATTCGTAAAGTAAATTTCCCAGCATCAAAAAATGAAGGATATGCCGTTGGATCTCGTAT
TTTATCAGTACAATTAATTATTTATAACCACTTCAATAAAATAATTTTCATTTAAAATAT
TG