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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFL353
Library AF
(Link to library)
Clone ID AFL353
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13862-1
Original site URL
Representative seq. ID AFL353P
(Link to Original site)
Representative DNA sequence 
>AFL353 (AFL353Q) /CSM/AF/AFL3-C/AFL353Q.Seq.d/
AAATTTATAAAATAAAAAATGAACAAGTATACATTGTTGCTATTACTTTTAAATACATTT
TTTATTTCAAATACTAAGTCTCAATCCTACAATGCAAATTGGACAGATTTAAATTCAAGA
CCAAATCCTGGATGGTATGATCAAGTTAAATTTGGAATTTTTATACATTTTGGAGTTTAT
AGTGTTCCAGCATTTGCAACCCATGATTATGCAGAATGGTATTGGTATAATTTAGTAAAT
GATAATGGTACAACTCAAAAATGGCAAGACCAACATTTTGGTGAACACTTTCAATATCAA
GATTTTGCACCAATGTTTAATTGTAGATTATTCAATCCAGATGAATGGGCATCAATATTT
CAAAAATCTGGTGCGAAATATGTTGTACTAACATCAAAACATCATGAAGGTTATACACTT
TGGAAAAGTGATCAATCATGGAGTTGGAATTCTGTTGAAAATGGTCCTGGAATTGATATT
GTAGGTAAXXXXXXXXXXAAACAGTTGTAGTAAATGATAGATGGGGTAAAGAGACAAGAG
GAGTTGATGGTGGGTTTTATACGGGTGGTGATAATTGGCAGCCTGGTCATTTGGTTACAC
ATAAATGGGAGAATTGTCAAACTATTGGTAGTTCATATGGTTATAATGAAAATGAACCAG
CAGAGGCATACCAAGATTCAACTATTTTAATTCAAGAATTTGTTGAAACAGTTGCATGTG
GTGGTAATTTCCTTTTAGATGTTGGACCAAATGCTGAAGGTGTAATTCCAAATAACATGG
TGGATCGTTTGTATGATATTGGTAAATTCTTATCAATTAATGGAGAATCAATTTATGGTA
GTTCTCCCTGGAGAATTCAAAATCAAACTCAATCAATTTGGTTCACTACAAATACTACGA
CAGGTGCAATCTATGCTTTCAGTTATTATTTTCCAGAAGATGGTGTATTTAATTTTAATG
AAATCATTGGTTCGAAATCTACAATTGTTGAATTATTCGGTTATAAAAGTAAAAATCCAA
TTACAATTAAACTTATCAACCAAAAGGAATTAACATAACTGTACCATTTGTATCACCATC
AGACATCCACCATT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
kfik*KMNKYTLLLLLLNTFFISNTKSQSYNANWTDLNSRPNPGWYDQVKFGIFIHFGVY
SVPAFATHDYAEWYWYNLVNDNGTTQKWQDQHFGEHFQYQDFAPMFNCRLFNPDEWASIF
QKSGAKYVVLTSKHHEGYTLWKSDQSWSWNSVENGPGIDIVG---

---TVVVNDRWGKETRGVDGGFYTGGDNWQPGHLVTHKWENCQTIGSSYGYNENEPAEAY
QDSTILIQEFVETVACGGNFLLDVGPNAEGVIPNNMVDRLYDIGKFLSINGESIYGSSPW
RIQNQTQSIWFTTNTTTGAIYAFSYYFPEDGVFNFNEIIGSKSTIVELFGYKSKNPITIK
LINQKELT*lyhlyhhqtsti
Translated Amino Acid sequence (All Frames) 
Frame A:
kfik*KMNKYTLLLLLLNTFFISNTKSQSYNANWTDLNSRPNPGWYDQVKFGIFIHFGVY
SVPAFATHDYAEWYWYNLVNDNGTTQKWQDQHFGEHFQYQDFAPMFNCRLFNPDEWASIF
QKSGAKYVVLTSKHHEGYTLWKSDQSWSWNSVENGPGIDIVG---

---kql**midgvkrqeelmvgfirvviigslviwlhingrivkllvvhmvimkmnqqrh
tkiqlf*fknllkqlhvvvisf*mldqmlkv*fqitwwivcmilvnsyqlmenqfmvvlp
gefkiklnqfgslqilrqvqsmlsviifqkmvylilmkslvrnlqllnysvikvkiqlql
nlstkrn*hncticitirhpp

Frame B:
nl*nkk*tsihccyyf*ihflfqilslnptmqigqi*iqdqildgmiklnleflyilefi
vfqhlqpmimqngigii**mimvqlkngktnilvntfnikilhqclivdysiqmnghqyf
knlvrnmly*hqnimkvihfgkvinhgvgillkmvlelil*v---

---nscsk**mg*rdkrs*wwvlygw**laawsfgyt*mgelsnyw*fiwl**k*tsrgi
prfnyfnsric*nscmww*fpfrcwtkc*rcnsk*hggsfv*yw*ilin*wrinlw*fsl
ensksnsinlvhykyydrcnlcfqllfsrrwci*f**nhwfeiync*iirl*k*ksnyn*
tyqpkginitvpfvspsdihh

Frame C:
iykikneqvyivaitfkyifyfky*vsilqckldrfkfktkswmv*ss*iwnfytfwsl*
cssicnp*lcrmvlv*fsk**wynskmarptfw*tlsisrfctnv*l*iiqsr*mginis
kiwceicctnikts*rlytlek*simelefc*kwswn*ycr*---

---TVVVNDRWGKETRGVDGGFYTGGDNWQPGHLVTHKWENCQTIGSSYGYNENEPAEAY
QDSTILIQEFVETVACGGNFLLDVGPNAEGVIPNNMVDRLYDIGKFLSINGESIYGSSPW
RIQNQTQSIWFTTNTTTGAIYAFSYYFPEDGVFNFNEIIGSKSTIVELFGYKSKNPITIK
LINQKELT*lyhlyhhqtsti
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL353 (AFL353Q) /CSM/AF/AFL3-C/AFL353Q.Seq.d/                       2109   0.0
AFK324 (AFK324Q) /CSM/AF/AFK3-A/AFK324Q.Seq.d/                       1168   0.0
AFJ332 (AFJ332Q) /CSM/AF/AFJ3-B/AFJ332Q.Seq.d/                       1168   0.0
CFA541 (CFA541Q) /CSM/CF/CFA5-B/CFA541Q.Seq.d/                       1166   0.0
VFG886 (VFG886Q) /CSM/VF/VFG8-D/VFG886Q.Seq.d/                       1154   0.0
VFA438 (VFA438Q) /CSM/VF/VFA4-B/VFA438Q.Seq.d/                       1154   0.0
SFG494 (SFG494Q) /CSM/SF/SFG4-D/SFG494Q.Seq.d/                       1154   0.0
SFF458 (SFF458Q) /CSM/SF/SFF4-C/SFF458Q.Seq.d/                       1154   0.0
SFE540 (SFE540Q) /CSM/SF/SFE5-B/SFE540Q.Seq.d/                       1154   0.0
CHD828 (CHD828Q) /CSM/CH/CHD8-B/CHD828Q.Seq.d/                       1154   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Y07497|Y07497.1 Dictyostelium discoideum putative alpha-L-fucosidase gene.   180   2e-93  7
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   180   1e-85  18
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    36   1e-06  17
AP004171|AP004171.1 Mycoplasma penetrans genomic DNA, complete sequence, section 2/5.    46   4e-06  12
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          32   9e-06  24
AC116551|AC116551.2 Dictyostelium discoideum chromosome 2 map complement(581327-427576) strain AX4, complete sequence.    34   6e-05  14
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           42   6e-05  21
AE017338|AE017338.1 Mycoplasma mobile 163K section 2 of 3 of the complete genome.    32   7e-05  17
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    34   9e-05  17
AC117081|AC117081.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    36   1e-04  17
dna update 2004. 8.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P10901) RecName: Full=Alpha-L-fucosidase;          EC=3.2.1.51;...   230   7e-59
BC078236_1(BC078236|pid:none) Danio rerio zgc:101116, mRNA (cDNA...   165   2e-39
BC050158_1(BC050158|pid:none) Danio rerio fucosidase, alpha-L- 1...   163   8e-39
BC066691_1(BC066691|pid:none) Danio rerio fucosidase, alpha-L- 1...   163   8e-39
CR942778_1(CR942778|pid:none) Xenopus tropicalis finished cDNA, ...   159   2e-37
CP000473_7005(CP000473|pid:none) Solibacter usitatus Ellin6076, ...   157   8e-37
AK170503_1(AK170503|pid:none) Mus musculus NOD-derived CD11c +ve...   155   2e-36
AK089958_1(AK089958|pid:none) Mus musculus kidney CCL-142 RAG cD...   154   6e-36
(Q99LJ1) RecName: Full=Tissue alpha-L-fucosidase;          EC=3....   154   6e-36
BC081844_1(BC081844|pid:none) Rattus norvegicus fucosidase, alph...   153   1e-35
protein update 2009. 4.25
PSORT 

 psg: 0.93  gvh: 0.65  alm: 0.38  top: 0.40  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: mitochondrial
	 16.0 %: nuclear
	  8.0 %: cytoplasmic
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: Golgi

>> prediction for AFL353 is exc
5' end seq. ID AFL353F
5' end seq. 
>AFL353F.Seq
AAATTTATAAAATAAAAAATGAACAAGTATACATTGTTGCTATTACTTTTAAATACATTT
TTTATTTCAAATACTAAGTCTCAATCCTACAATGCAAATTGGACAGATTTAAATTCAAGA
CCAAATCCTGGATGGTATGATCAAGTTAAATTTGGAATTTTTATACATTTTGGAGTTTAT
AGTGTTCCAGCATTTGCAACCCATGATTATGCAGAATGGTATTGGTATAATTTAGTAAAT
GATAATGGTACAACTCAAAAATGGCAAGACCAACATTTTGGTGAACACTTTCAATATCAA
GATTTTGCACCAATGTTTAATTGTAGATTATTCAATCCAGATGAATGGGCATCAATATTT
CAAAAATCTGGTGCGAAATATGTTGTACTAACATCAAAACATCATGAAGGTTATACACTT
TGGAAAAGTGATCAATCATGGAGTTGGAATTCTGTTGAAAATGGTCCTGGAATTGATATT
GTAGGTAA----------
Length of 5' end seq. 488
3' end seq. ID AFL353Z
3' end seq. 
>AFL353Z.Seq
----------AAACAGTTGTAGTAAATGATAGATGGGGTAAAGAGACAAGAGGAGTTGAT
GGTGGGTTTTATACGGGTGGTGATAATTGGCAGCCTGGTCATTTGGTTACACATAAATGG
GAGAATTGTCAAACTATTGGTAGTTCATATGGTTATAATGAAAATGAACCAGCAGAGGCA
TACCAAGATTCAACTATTTTAATTCAAGAATTTGTTGAAACAGTTGCATGTGGTGGTAAT
TTCCTTTTAGATGTTGGACCAAATGCTGAAGGTGTAATTCCAAATAACATGGTGGATCGT
TTGTATGATATTGGTAAATTCTTATCAATTAATGGAGAATCAATTTATGGTAGTTCTCCC
TGGAGAATTCAAAATCAAACTCAATCAATTTGGTTCACTACAAATACTACGACAGGTGCA
ATCTATGCTTTCAGTTATTATTTTCCAGAAGATGGTGTATTTAATTTTAATGAAATCATT
GGTTCGAAATCTACAATTGTTGAATTATTCGGTTATAAAAGTAAAAATCCAATTACAATT
AAACTTATCAACCAAAAGGAATTAACATAACTGTACCATTTGTATCACCATCAGACATCC
ACCATT
Length of 3' end seq. 596
Connected seq. ID AFL353P
Connected seq. 
>AFL353P.Seq
AAATTTATAAAATAAAAAATGAACAAGTATACATTGTTGCTATTACTTTTAAATACATTT
TTTATTTCAAATACTAAGTCTCAATCCTACAATGCAAATTGGACAGATTTAAATTCAAGA
CCAAATCCTGGATGGTATGATCAAGTTAAATTTGGAATTTTTATACATTTTGGAGTTTAT
AGTGTTCCAGCATTTGCAACCCATGATTATGCAGAATGGTATTGGTATAATTTAGTAAAT
GATAATGGTACAACTCAAAAATGGCAAGACCAACATTTTGGTGAACACTTTCAATATCAA
GATTTTGCACCAATGTTTAATTGTAGATTATTCAATCCAGATGAATGGGCATCAATATTT
CAAAAATCTGGTGCGAAATATGTTGTACTAACATCAAAACATCATGAAGGTTATACACTT
TGGAAAAGTGATCAATCATGGAGTTGGAATTCTGTTGAAAATGGTCCTGGAATTGATATT
GTAGGTAA----------AAACAGTTGTAGTAAATGATAGATGGGGTAAAGAGACAAGAG
GAGTTGATGGTGGGTTTTATACGGGTGGTGATAATTGGCAGCCTGGTCATTTGGTTACAC
ATAAATGGGAGAATTGTCAAACTATTGGTAGTTCATATGGTTATAATGAAAATGAACCAG
CAGAGGCATACCAAGATTCAACTATTTTAATTCAAGAATTTGTTGAAACAGTTGCATGTG
GTGGTAATTTCCTTTTAGATGTTGGACCAAATGCTGAAGGTGTAATTCCAAATAACATGG
TGGATCGTTTGTATGATATTGGTAAATTCTTATCAATTAATGGAGAATCAATTTATGGTA
GTTCTCCCTGGAGAATTCAAAATCAAACTCAATCAATTTGGTTCACTACAAATACTACGA
CAGGTGCAATCTATGCTTTCAGTTATTATTTTCCAGAAGATGGTGTATTTAATTTTAATG
AAATCATTGGTTCGAAATCTACAATTGTTGAATTATTCGGTTATAAAAGTAAAAATCCAA
TTACAATTAAACTTATCAACCAAAAGGAATTAACATAACTGTACCATTTGTATCACCATC
AGACATCCACCATT
Length of connected seq. 1084
Full length Seq ID -
Full length Seq. -
Length of full length seq. -