>VFA147 (VFA147Q) /CSM/VF/VFA1-B/VFA147Q.Seq.d/
XXXXXXXXXXAACAAGAGTGTTACAAAATGCCTCAATACAAAATTAGTTTGGATAATACC
AATGATCCAATAATTACGCCAACAATTACACCANCTCTTCTACATTTCCAACAANACACC
AACTCGNATACGCCAACAACAACTGCACCAACTNCACCAACGCCAACACCAACACC

---QECYKMPQYKISLDNTNDPIITPTITPXLLHFQQXTNSXTPTTTAPTXPTPTPT

Frame A:
---trvlqnasiqn*fg*yq*snnyannytxsstfptxhqlxyannnctnxtnantnt


Frame B:
---QECYKMPQYKISLDNTNDPIITPTITPXLLHFQQXTNSXTPTTTAPTXPTPTPT


Frame C:
---ksvtkclntklvwiipmiq*lrqqlhxlfyisnxtptrirqqqlhqlhqrqhqh

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA147 (VFA147Q) /CSM/VF/VFA1-B/VFA147Q.Seq.d/                        305   1e-82
VFA370 (VFA370Q) /CSM/VF/VFA3-C/VFA370Q.Seq.d/                         42   0.003
AFF855 (AFF855Q) /CSM/AF/AFF8-C/AFF855Q.Seq.d/                         42   0.003
AFA228 (AFA228Q) /CSM/AF/AFA2-B/AFA228Q.Seq.d/                         42   0.003
VHP772 (VHP772Q) /CSM/VH/VHP7-C/VHP772Q.Seq.d/                         36   0.16
VHP575 (VHP575Q) /CSM/VH/VHP5-D/VHP575Q.Seq.d/                         36   0.16
VHI727 (VHI727Q) /CSM/VH/VHI7-B/VHI727Q.Seq.d/                         36   0.16
VHD362 (VHD362Q) /CSM/VH/VHD3-C/VHD362Q.Seq.d/                         36   0.16
VHA380 (VHA380Q) /CSM/VH/VHA3-D/VHA380Q.Seq.d/                         36   0.16
SLF558 (SLF558Q) /CSM/SL/SLF5-C/SLF558Q.Seq.d/                         36   0.16

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF129455|AF129455.1 Simian immunodeficiency virus clone SIV1480-AconV1 envelope glycoprotein (env) gene, partial cds.    50  0.008  1
BG600810|BG600810.1 EST505705 cSTS Solanum tuberosum cDNA clone cSTS30I17 5' sequence, mRNA sequence.    44  0.52  1
BQ873987|BQ873987.1 QGI4B16.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGI4B16, mRNA sequence.    44  0.52  1
BI930401|BI930401.1 EST550278 tomato flower, 8 mm to preanthesis buds Lycopersicon esculentum cDNA clone cTOC16K17 5' end, mRNA sequence.    44  0.52  1
BQ982208|BQ982208.1 QGE15N09.yg.ab1 QG_EFGHJ lettuce serriola Lactuca sativa cDNA clone QGE15N09, mRNA sequence.    44  0.52  1
BM110566|BM110566.1 EST558102 potato roots Solanum tuberosum cDNA clone cPRO8F1 5' end, mRNA sequence.    44  0.52  1
BI931581|BI931581.1 EST551482 tomato flower, 8 mm to preanthesis buds Lycopersicon esculentum cDNA clone cTOC20D9 5' end, mRNA sequence.    44  0.52  1
BQ993070|BQ993070.1 QGF27I02.yg.ab1 QG_EFGHJ lettuce serriola Lactuca sativa cDNA clone QGF27I02, mRNA sequence.    44  0.52  1
BI504258|BI504258.1 BB170001B10B07 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170001B10B07 5', mRNA sequence.    36  1.3  2
BI503651|BI503651.1 BB170019A20G09 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170019A20G09 5', mRNA sequence.    36  1.7  2

 ***** No hits found ******

Lambda     K      H
   0.315    0.128    0.381

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3204285
Number of Hits to DB: 161,278,084

 psg: 0.54  gvh: 0.46  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 24.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFA147 is cyt

>VFA147Z.Seq
----------AACAAGAGTGTTACAAAATGCCTCAATACAAAATTAGTTTGGATAATACC
AATGATCCAATAATTACGCCAACAATTACACCANCTCTTCTACATTTCCAACAANACACC
AACTCGNATACGCCAACAACAACTGCACCAACTNCACCAACGCCAACACCAACACC