NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA226
Library VF
(Link to library)
Clone ID VFA226
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15522-1
Original site URL
Representative seq. ID VFA226P
(Link to Original site)
Representative DNA sequence 
>VFA226 (VFA226Q) /CSM/VF/VFA2-B/VFA226Q.Seq.d/
AACATGGCAGAAAATATAATAATAATAATAATAATAATAATAATAATGTTTCAACACCAC
CACATCAAAAACCACATTTAACAACTGGTTTAAGAACTTCAAGTTCTGGTTTATTAATGG
ATAAAAGAAGACAAGATGAAGAAAAATTTTCAGCTGAACAAGTAGCAATGGGTAAAAAAT
CACCAACAAAATTATTCTCAGCATACAGCTTAAATGATTTATCAAATCCACCAACAGATG
AAGAATTAAATAACTATAAGGATTTACCAGCATCAAGTCTTCCACCAATTCATAAAAGAG
AGAAATTAAGAAGAGTATTTGTAAATAGAGATATTAAACTCGACAGAATTGAATTCTTTG
GTTTTGATATGGATTATACATTAGCAGTTTATAATTCACCAGATTTTGAAGAGTTGGCAT
ATGATATGGTACTTGATAAACTCATTGATATTGGTTATCCAAAATCAATTAGAAAATTAA
AATATGATCCAAATTTCCCAACTAGAGGTTTATTTTTAGATAGAGAACTTGGTAACCTTT
TAAAAATTGATAGTTTTGGTAACATTATCATTTGCGTTCATGGTAGAACAACCTTTATCA
AAAAAXXXXXXXXXXAGTTTATCATGGTGGTTCTTTATCATTATTCCAGAAAATTAACAG
GAGCAAAAGGTAGTAGAGTACTTTACATTGGTGATCATATCTTTGCAGATATCATTAAAT
CAAAGAAAACTCATGGATGGAGAAATCTATTGGTTGTACCAGAACTTCAACATGAATTAG
AGGTTATGAATCAACAAAAAGATACAACCATTCATTTGATGAATCTTGAATTCATTAGAG
CTGAAATCTATAGAGGCTTAGATTCTGAATCAACCACCCCACCAGATATTGAAGTTCTCC
ATCAACATCTTAAAAATACAAATGATAAATTGAACTTTGCCTACAACAAATACTTTGGTT
CCCTCTTTAAAAATGGTTCAAAATCAACTTTCTTCTCTATGCAAGTTCAAAGATATGCTG
ATCTTTACACCTCTGATTATCTCAATCTCTTAAACTATCCATTATTTTATCATTTTTGTG
CAAATAGTTTACCTCTTCCACATGAAAGTTCTTCTTTCTCTTCTTTNATACAAGTAATTA
AATAAAATAAAAGAAAATAAAACAAAATAAAAAAACAAATAAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
HGRKYNNNNNNNNNNVSTPPHQKPHLTTGLRTSSSGLLMDKRRQDEEKFSAEQVAMGKKS
PTKLFSAYSLNDLSNPPTDEELNNYKDLPASSLPPIHKREKLRRVFVNRDIKLDRIEFFG
FDMDYTLAVYNSPDFEELAYDMVLDKLIDIGYPKSIRKLKYDPNFPTRGLFLDRELGNLL
KIDSFGNIIICVHGRTTFIKK---

---FIMVVLYHYSRKLTGAKGSRVLYIGDHIFADIIKSKKTHGWRNLLVVPELQHELEVM
NQQKDTTIHLMNLEFIRAEIYRGLDSESTTPPDIEVLHQHLKNTNDKLNFAYNKYFGSLF
KNGSKSTFFSMQVQRYADLYTSDYLNLLNYPLFYHFCANSLPLPHESSSFSSXIQVIK*n
krk*nkikkqik
Translated Amino Acid sequence (All Frames) 
Frame A:
nmaeniiiiiiiiiimfqhhhiknhi*qlv*elqvlvy*wikedkmkknfqlnk*qwvkn
hqqnysqhta*miyqihqqmkn*itiriyqhqvfhqfikern*eeyl*ieilnstelnsl
vliwiih*qfiihqilkswhmiwylinslilviqnqlen*nmiqisqlevyf*ienlvtf
*klivlvtlsfafmveqplsk---

---slswwffiiipen*qeqkvveyftlviislqislnqrklmdgeiywlyqnfnmn*rl
*inkkiqpfi**ilnslelksiea*ilnqpphqilkfsinilkiqmin*tlpttntlvps
lkmvqnqlsslckfkdmliftpliisis*tihyfiifvqivylfhmkvllsllxyk*lnk
ikenktk*knk*k

Frame B:
twqki**********cfntttskttfnnwfknfkfwfing*kktr*rkifs*tssng*ki
tnkiilsiqlk*fikstnr*rik*l*gftsiksstns*kreikksick*ry*trqn*ilw
f*yglyissl*ftrf*rvgi*ygt**th*ywlskin*kiki*skfpn*rfifr*rtw*pf
kn**fw*hyhlrsw*nnlyqk---

---vyhggslslfqkinrskr**stlhw*sylcryh*ikenswmeksigctrtst*irgy
estkrynhsfdes*ih*s*nl*rlrf*inhptry*sspsts*kyk**ielclqqilwfpl
*kwfkinfllyasskic*slhl*lsqslklsiilsflck*ftsst*kffflffxtsn*ik
*kkikqnkktnk

Frame C:
HGRKYNNNNNNNNNNVSTPPHQKPHLTTGLRTSSSGLLMDKRRQDEEKFSAEQVAMGKKS
PTKLFSAYSLNDLSNPPTDEELNNYKDLPASSLPPIHKREKLRRVFVNRDIKLDRIEFFG
FDMDYTLAVYNSPDFEELAYDMVLDKLIDIGYPKSIRKLKYDPNFPTRGLFLDRELGNLL
KIDSFGNIIICVHGRTTFIKK---

---FIMVVLYHYSRKLTGAKGSRVLYIGDHIFADIIKSKKTHGWRNLLVVPELQHELEVM
NQQKDTTIHLMNLEFIRAEIYRGLDSESTTPPDIEVLHQHLKNTNDKLNFAYNKYFGSLF
KNGSKSTFFSMQVQRYADLYTSDYLNLLNYPLFYHFCANSLPLPHESSSFSSXIQVIK*n
krk*nkikkqik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA226 (VFA226Q) /CSM/VF/VFA2-B/VFA226Q.Seq.d/                       2097   0.0
AFG556 (AFG556Q) /CSM/AF/AFG5-C/AFG556Q.Seq.d/                       1090   0.0
AFJ814 (AFJ814Q) /CSM/AF/AFJ8-A/AFJ814Q.Seq.d/                       1082   0.0
CFI768 (CFI768Q) /CSM/CF/CFI7-C/CFI768Q.Seq.d/                       1065   0.0
SFJ610 (SFJ610Q) /CSM/SF/SFJ6-A/SFJ610Q.Seq.d/                       1037   0.0
AFJ789 (AFJ789Q) /CSM/AF/AFJ7-D/AFJ789Q.Seq.d/                       1037   0.0
SFE201 (SFE201Q) /CSM/SF/SFE2-A/SFE201Q.Seq.d/                       1015   0.0
VFE176 (VFE176Q) /CSM/VF/VFE1-D/VFE176Q.Seq.d/                       1009   0.0
AFF109 (AFF109Q) /CSM/AF/AFF1-A/AFF109Q.Seq.d/                       1009   0.0
CFA788 (CFA788Q) /CSM/CF/CFA7-D/CFA788Q.Seq.d/                       1001   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC120206|AC120206.2 Canis familiaris clone RP81-219C14, WORKING DRAFT SEQUENCE, 7 ordered pieces.    56  0.022  2
AC117531|AC117531.2 Homo sapiens chromosome 5 clone RP11-603O17, complete sequence.    42  0.030  5
CC008913|CC008913.1 PUDEI80TD ZM_0.6_1.0_KB Zea mays genomic clone ZMMBTa168M15, DNA sequence.    44  0.039  2
BB845391|BB845391.1 Mus musculus adult male kidney cDNA, RIKEN full-length enriched library, clone:F520005H13, 5' end partial sequence.    46  0.052  2
BB392891|BB392891.1 Mus musculus 0 day neonate cerebellum cDNA, RIKEN full-length enriched library, clone:C230081A07, 3' end partial sequence.    50  0.064  1
AC108322|AC108322.4 Rattus norvegicus clone CH230-261G16, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    50  0.064  1
AC020893|AC020893.6 Homo sapiens chromosome 5 clone CTC-250P20, complete sequence.    44  0.091  6
AC035141|AC035141.3 Homo sapiens chromosome 5 clone CTD-2010H15, WORKING DRAFT SEQUENCE, 10 ordered pieces.    44  0.11  5
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.          34  0.17  13
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    44  0.18  9
dna update 2003. 8.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54XC1) RecName: Full=Cytosolic purine 5'-nucleotidase;        ...   372   e-101
BC064760_1(BC064760|pid:none) Mus musculus 5'-nucleotidase, cyto...   146   2e-33
AK036730_1(AK036730|pid:none) Mus musculus adult male bone cDNA,...   146   2e-33
AK132384_1(AK132384|pid:none) Mus musculus 15 days embryo head c...   146   2e-33
(Q3V1L4) RecName: Full=Cytosolic purine 5'-nucleotidase;        ...   146   2e-33
(Q5EBF1) RecName: Full=Cytosolic purine 5'-nucleotidase;        ...   146   2e-33
AK291667_1(AK291667|pid:none) Homo sapiens cDNA FLJ75877 complet...   146   2e-33
AB168644_1(AB168644|pid:none) Macaca fascicularis testis cDNA cl...   146   2e-33
AK295593_1(AK295593|pid:none) Homo sapiens cDNA FLJ53335 complet...   146   2e-33
(Q5ZIZ4) RecName: Full=Cytosolic purine 5'-nucleotidase;        ...   145   3e-33
protein update 2009. 6.16
PSORT 

 psg: 0.67  gvh: 0.39  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.42  mip: 0.06
 nuc: 0.03  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 12.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for VFA226 is mit
5' end seq. ID VFA226F
5' end seq. 
>VFA226F.Seq
AACATGGCAGAAAATATAATAATAATAATAATAATAATAATAATAATGTTTCAACACCAC
CACATCAAAAACCACATTTAACAACTGGTTTAAGAACTTCAAGTTCTGGTTTATTAATGG
ATAAAAGAAGACAAGATGAAGAAAAATTTTCAGCTGAACAAGTAGCAATGGGTAAAAAAT
CACCAACAAAATTATTCTCAGCATACAGCTTAAATGATTTATCAAATCCACCAACAGATG
AAGAATTAAATAACTATAAGGATTTACCAGCATCAAGTCTTCCACCAATTCATAAAAGAG
AGAAATTAAGAAGAGTATTTGTAAATAGAGATATTAAACTCGACAGAATTGAATTCTTTG
GTTTTGATATGGATTATACATTAGCAGTTTATAATTCACCAGATTTTGAAGAGTTGGCAT
ATGATATGGTACTTGATAAACTCATTGATATTGGTTATCCAAAATCAATTAGAAAATTAA
AATATGATCCAAATTTCCCAACTAGAGGTTTATTTTTAGATAGAGAACTTGGTAACCTTT
TAAAAATTGATAGTTTTGGTAACATTATCATTTGCGTTCATGGTAGAACAACCTTTATCA
AAAAA----------
Length of 5' end seq. 605
3' end seq. ID VFA226Z
3' end seq. 
>VFA226Z.Seq
----------AGTTTATCATGGTGGTTCTTTATCATTATTCCAGAAAATTAACAGGAGCA
AAAGGTAGTAGAGTACTTTACATTGGTGATCATATCTTTGCAGATATCATTAAATCAAAG
AAAACTCATGGATGGAGAAATCTATTGGTTGTACCAGAACTTCAACATGAATTAGAGGTT
ATGAATCAACAAAAAGATACAACCATTCATTTGATGAATCTTGAATTCATTAGAGCTGAA
ATCTATAGAGGCTTAGATTCTGAATCAACCACCCCACCAGATATTGAAGTTCTCCATCAA
CATCTTAAAAATACAAATGATAAATTGAACTTTGCCTACAACAAATACTTTGGTTCCCTC
TTTAAAAATGGTTCAAAATCAACTTTCTTCTCTATGCAAGTTCAAAGATATGCTGATCTT
TACACCTCTGATTATCTCAATCTCTTAAACTATCCATTATTTTATCATTTTTGTGCAAAT
AGTTTACCTCTTCCACATGAAAGTTCTTCTTTCTCTTCTTTNATACAAGTAATTAAATAA
AATAAAAGAAAATAAAACAAAATAAAAAAACAAATAAAAA
Length of 3' end seq. 570
Connected seq. ID VFA226P
Connected seq. 
>VFA226P.Seq
AACATGGCAGAAAATATAATAATAATAATAATAATAATAATAATAATGTTTCAACACCAC
CACATCAAAAACCACATTTAACAACTGGTTTAAGAACTTCAAGTTCTGGTTTATTAATGG
ATAAAAGAAGACAAGATGAAGAAAAATTTTCAGCTGAACAAGTAGCAATGGGTAAAAAAT
CACCAACAAAATTATTCTCAGCATACAGCTTAAATGATTTATCAAATCCACCAACAGATG
AAGAATTAAATAACTATAAGGATTTACCAGCATCAAGTCTTCCACCAATTCATAAAAGAG
AGAAATTAAGAAGAGTATTTGTAAATAGAGATATTAAACTCGACAGAATTGAATTCTTTG
GTTTTGATATGGATTATACATTAGCAGTTTATAATTCACCAGATTTTGAAGAGTTGGCAT
ATGATATGGTACTTGATAAACTCATTGATATTGGTTATCCAAAATCAATTAGAAAATTAA
AATATGATCCAAATTTCCCAACTAGAGGTTTATTTTTAGATAGAGAACTTGGTAACCTTT
TAAAAATTGATAGTTTTGGTAACATTATCATTTGCGTTCATGGTAGAACAACCTTTATCA
AAAAA----------AGTTTATCATGGTGGTTCTTTATCATTATTCCAGAAAATTAACAG
GAGCAAAAGGTAGTAGAGTACTTTACATTGGTGATCATATCTTTGCAGATATCATTAAAT
CAAAGAAAACTCATGGATGGAGAAATCTATTGGTTGTACCAGAACTTCAACATGAATTAG
AGGTTATGAATCAACAAAAAGATACAACCATTCATTTGATGAATCTTGAATTCATTAGAG
CTGAAATCTATAGAGGCTTAGATTCTGAATCAACCACCCCACCAGATATTGAAGTTCTCC
ATCAACATCTTAAAAATACAAATGATAAATTGAACTTTGCCTACAACAAATACTTTGGTT
CCCTCTTTAAAAATGGTTCAAAATCAACTTTCTTCTCTATGCAAGTTCAAAGATATGCTG
ATCTTTACACCTCTGATTATCTCAATCTCTTAAACTATCCATTATTTTATCATTTTTGTG
CAAATAGTTTACCTCTTCCACATGAAAGTTCTTCTTTCTCTTCTTTNATACAAGTAATTA
AATAAAATAAAAGAAAATAAAACAAAATAAAAAAACAAATAAAAA
Length of connected seq. 1175
Full length Seq ID -
Full length Seq. -
Length of full length seq. -