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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA255
Library VF
(Link to library)
Clone ID VFA255
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID VFA255P
(Link to Original site)
Representative DNA sequence 
>VFA255 (VFA255Q) /CSM/VF/VFA2-C/VFA255Q.Seq.d/
AAATTTATTTNCAAAAAGTATAATACAAGATGGGTAAAGAAAAAACACATATTAATATCG
TCGTTATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAAT
GTGGTGGTATCGATAAAAGAGTTATCGAAAAATACGAAAAGGAAGCTTCTGAAATGGGTA
AACAATCATTCAAATATGCTTGGGTTATGGATAAATTAAAAGCTGAACGTGAAAGAGGTA
TTACCATCGATATTGCTTTATGGAAATTCGAAACCTCAAAATACTACTTCACCATTATTG
ATGCTCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCACAAGCCGATT
GTGCTGTCTTAGTCATTGCCTCCCCAACTGGTGAGTTCGAAGCTGGTATTGCCAAGAACG
GTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTTG
CTATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGACGAAATTG
TCAAAGAAGTTTCATCTTTCATTAXXXXXXXXXXCAATGTAGGTTTCAACGGTTAAAAAC
GTTTCAGTCAAAGAAATTTAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCCCCN
CAAGNAACTNAAAAATTCGTTGNTCAAGTTTTNGTCCTCAACCATNCAGGTCAAATCCAT
GCTGGTTACTCACCAGTNTTAGATTGTCNCACTGCTCACATTGCCTGTNAATTCACTGAA
ATNGTCGATAAAGTTGATCGTNGTACTGGTGCCGTCGTTGCCAAANAAGGTACTGCCNCC
GTCGTNTTAAAGAATGGTGATGCTGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGT
GTTGAATCATTCNCTGAATACCCNCCATTAGGTCGTTTCGCCGTCANANATATNAGACAA
ACCGTTGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCAGGTTAT
AANAAAGGTGCTGCTGCTCCATCAAAGAAGAAATAAATATTTAAAAAAAATTTTATATTT
TCTAAAANAAAAAAAAAAAATAAAAAAGAGCTTTTA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
iyxqkv*YKMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMGK
QSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQADC
AVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYDEIV
KEVSSFI---

---QCRFQRLKTFQSKKFKEVWSLVTPKTIPXKXLKNSLXKFXSSTXQVKSMLVTHQX*i
vxlltlpvnslkxsiklivvlvpslpxkvlppsx*rmvmllwsn*phlvqcvlnhslntx
h*vvspsxixdkplpsvssnqpsrkpqvkqvixkvlllhqrrnkylkkilyflkxkkkik
ksf
Translated Amino Acid sequence (All Frames) 
Frame A:
kfixkkyntrwvkkkhilissllvt*mlvnqpplvi*stnvvvsikelskntkrkllkwv
nnhsnmlglwin*klnvkevlpsillygnskpqnttspllmlqvteissri*llvphkpi
vls*slppqlvssklvlprtvklvntlslltl*vsnk*sllstrwmknqptthkpvttkl
skkfhlsl---

---QCRFQRLKTFQSKKFKEVWSLVTPKTIPXKXLKNSLXKFXSSTXQVKSMLVTHQX*i
vxlltlpvnslkxsiklivvlvpslpxkvlppsx*rmvmllwsn*phlvqcvlnhslntx
h*vvspsxixdkplpsvssnqpsrkpqvkqvixkvlllhqrrnkylkkilyflkxkkkik
ksf

Frame B:
nlfxksiiqdg*rknty*yrrywsrrcw*inhhwsfnlqmwwyr*ksyrkirkgsf*ng*
tiiqiclgyg*iks*t*kryyhrycfmeirnlkillhhy*csrsqrfhqeydywyltsrl
cclshclpnw*vrswycqersns*trspclhfrcqtndrcyqqdg*kinqlltsplrrnc
qrsfifh---

---nvgfng*krfsqrnlkrygrw*lqkrspxxnxkirxssfxpqpxrsnpcwlltsxrl
shcshclxih*nxr*s*sxywcrrcqxrycxrrxkew*ccygrinpissnvc*iix*ipx
irsfrrxxyxtnrcrrchqinrqespr*srl*xrcccsikeeini*kkfyif*xkkkk*k
raf

Frame C:
iyxqkv*YKMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMGK
QSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQADC
AVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYDEIV
KEVSSFI---

---m*vstvknvsvkei*rgmvagdskndppqxtxkfvxqvxvlnhxgqihagyspvldc
xtahiacxftexvdkvdrxtgavvakxgtaxvvlkngdaamveltpsrpmcvesfxeypp
lgrfavxxxrqtvavgvikstvkkapgkagyxkgaaapskkk*ifkknfifskxkkknkk
ell
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA255 (VFA255Q) /CSM/VF/VFA2-C/VFA255Q.Seq.d/                       1921   0.0
VFA282 (VFA282Q) /CSM/VF/VFA2-D/VFA282Q.Seq.d/                       1112   0.0
CFI165 (CFI165Q) /CSM/CF/CFI1-C/CFI165Q.Seq.d/                       1112   0.0
CFH210 (CFH210Q) /CSM/CF/CFH2-A/CFH210Q.Seq.d/                       1112   0.0
CFB884 (CFB884Q) /CSM/CF/CFB8-D/CFB884Q.Seq.d/                       1112   0.0
CFB539 (CFB539Q) /CSM/CF/CFB5-B/CFB539Q.Seq.d/                       1112   0.0
CFA262 (CFA262Q) /CSM/CF/CFA2-C/CFA262Q.Seq.d/                       1112   0.0
VFA332 (VFA332Q) /CSM/VF/VFA3-B/VFA332Q.Seq.d/                       1110   0.0
VFA252 (VFA252Q) /CSM/VF/VFA2-C/VFA252Q.Seq.d/                       1110   0.0
VFA710 (VFA710Q) /CSM/VF/VFA7-A/VFA710Q.Seq.d/                       1104   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.  1043  0.0  3
X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.   858  0.0  3
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.   769  0.0  4
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   260  e-122  7
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   236  e-115  7
AF402092|AF402092.1 Pichia anomala strain NRRL Y-366 translation elongation factor 1-alpha gene, partial cds.   224  e-103  6
AF402023|AF402023.1 Kluyveromyces piceae strain NRRL Y-17977 translation elongation factor 1-alpha gene, partial cds.   204  3e-98  5
AX109715|AX109715.1 Sequence 448 from Patent WO0123604.          192  3e-95  7
AX109714|AX109714.1 Sequence 447 from Patent WO0123604.          188  4e-94  7
AF402018|AF402018.1 Saccharomyces unisporus strain NRRL Y-1556 translation elongation factor 1-alpha gene, partial cds.   109  2e-92  6
dna update 2003. 8.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   358   1e-97
X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   352   1e-95
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   352   1e-95
BC092884_1(BC092884|pid:none) Danio rerio zgc:110335, mRNA (cDNA...   318   3e-85
(P13549) RecName: Full=Elongation factor 1-alpha, somatic form; ...   317   4e-85
(Q90835) RecName: Full=Elongation factor 1-alpha 1;          Sho...   317   5e-85
AK167843_1(AK167843|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   317   5e-85
AB174733_1(AB174733|pid:none) Macaca fascicularis brain cDNA clo...   317   5e-85
(P10126) RecName: Full=Elongation factor 1-alpha 1;          Sho...   317   5e-85
M22432_1(M22432|pid:none) Mus musculus protein synthesis elongat...   317   5e-85
protein update 2009. 6.16
PSORT 

 psg: 0.84  gvh: 0.34  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VFA255 is cyt
5' end seq. ID VFA255F
5' end seq. 
>VFA255F.Seq
AAATTTATTTNCAAAAAGTATAATACAAGATGGGTAAAGAAAAAACACATATTAATATCG
TCGTTATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAAT
GTGGTGGTATCGATAAAAGAGTTATCGAAAAATACGAAAAGGAAGCTTCTGAAATGGGTA
AACAATCATTCAAATATGCTTGGGTTATGGATAAATTAAAAGCTGAACGTGAAAGAGGTA
TTACCATCGATATTGCTTTATGGAAATTCGAAACCTCAAAATACTACTTCACCATTATTG
ATGCTCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCACAAGCCGATT
GTGCTGTCTTAGTCATTGCCTCCCCAACTGGTGAGTTCGAAGCTGGTATTGCCAAGAACG
GTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTTG
CTATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGACGAAATTG
TCAAAGAAGTTTCATCTTTCATTA----------
Length of 5' end seq. 564
3' end seq. ID VFA255Z
3' end seq. 
>VFA255Z.Seq
----------CAATGTAGGTTTCAACGGTTAAAAACGTTTCAGTCAAAGAAATTTAAAGA
GGTATGGTCGCTGGTGACTCCAAAAACGATCCCCCNCAAGNAACTNAAAAATTCGTTGNT
CAAGTTTTNGTCCTCAACCATNCAGGTCAAATCCATGCTGGTTACTCACCAGTNTTAGAT
TGTCNCACTGCTCACATTGCCTGTNAATTCACTGAAATNGTCGATAAAGTTGATCGTNGT
ACTGGTGCCGTCGTTGCCAAANAAGGTACTGCCNCCGTCGTNTTAAAGAATGGTGATGCT
GCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCATTCNCTGAATACCCN
CCATTAGGTCGTTTCGCCGTCANANATATNAGACAAACCGTTGCCGTCGGTGTCATCAAA
TCAACCGTCAAGAAAGCCCCAGGTAAAGCAGGTTATAANAAAGGTGCTGCTGCTCCATCA
AAGAAGAAATAAATATTTAAAAAAAATTTTATATTTTCTAAAANAAAAAAAAAAAATAAA
AAAGAGCTTTTA
Length of 3' end seq. 542
Connected seq. ID VFA255P
Connected seq. 
>VFA255P.Seq
AAATTTATTTNCAAAAAGTATAATACAAGATGGGTAAAGAAAAAACACATATTAATATCG
TCGTTATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAAT
GTGGTGGTATCGATAAAAGAGTTATCGAAAAATACGAAAAGGAAGCTTCTGAAATGGGTA
AACAATCATTCAAATATGCTTGGGTTATGGATAAATTAAAAGCTGAACGTGAAAGAGGTA
TTACCATCGATATTGCTTTATGGAAATTCGAAACCTCAAAATACTACTTCACCATTATTG
ATGCTCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCACAAGCCGATT
GTGCTGTCTTAGTCATTGCCTCCCCAACTGGTGAGTTCGAAGCTGGTATTGCCAAGAACG
GTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTTG
CTATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGACGAAATTG
TCAAAGAAGTTTCATCTTTCATTA----------CAATGTAGGTTTCAACGGTTAAAAAC
GTTTCAGTCAAAGAAATTTAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCCCCN
CAAGNAACTNAAAAATTCGTTGNTCAAGTTTTNGTCCTCAACCATNCAGGTCAAATCCAT
GCTGGTTACTCACCAGTNTTAGATTGTCNCACTGCTCACATTGCCTGTNAATTCACTGAA
ATNGTCGATAAAGTTGATCGTNGTACTGGTGCCGTCGTTGCCAAANAAGGTACTGCCNCC
GTCGTNTTAAAGAATGGTGATGCTGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGT
GTTGAATCATTCNCTGAATACCCNCCATTAGGTCGTTTCGCCGTCANANATATNAGACAA
ACCGTTGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCAGGTTAT
AANAAAGGTGCTGCTGCTCCATCAAAGAAGAAATAAATATTTAAAAAAAATTTTATATTT
TCTAAAANAAAAAAAAAAAATAAAAAAGAGCTTTTA
Length of connected seq. 1106
Full length Seq ID -
Full length Seq. -
Length of full length seq. -