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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA896
Library VF
(Link to library)
Clone ID VFA896
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15331-1
Original site URL
Representative seq. ID VFA896P
(Link to Original site)
Representative DNA sequence 
>VFA896 (VFA896Q) /CSM/VF/VFA8-D/VFA896Q.Seq.d/
GTAGTTTTTAAGTAATATTTCACACAATATATAAAATGTCATCTTATTTATTAACTAGTG
AATCCGTCACCGAAGGTCATCCAGATAAAATCTGTGATCAAGTATCAGATGCTGTTCTCG
ATGCTTGTTTAGCTCAAGATCCATTATCAAAAGTTGCTTGTGAAACTGCAACCAAAACTG
GTATGGTTATGATTTTAGGTGAAATTACCACCAAAGCCAACGTTGACTATCAAAAAGTTG
TTCGTGAAGCTGTTAAAAAGATTGGTTATGATGACTCATCAAAAGGTTTTGATTACAAAA
CTTGCAATGTTTTAGTTGCAATTGAACAACAATCACCAGATATTGCTCAAGGTGTCCACA
TTGGTAAAGTTAACCCAGAAGATATTGGTGCTGGTGATCAAGGTCACATGTTTGGTTATG
CCACCAACGAAACCCCAACTTTAATGCCACTTACTCATTTCCTCGCCTCTGAATTAGTCA
ACAAATTAACTGAATTACGTCACAATGGTACCTTAGCCTGGGCTCGTCCAGATGCCAAGA
CTCAAGTCACTGTTGAATACAAAAAGGAAGGTCACAAAATCACTCCAGTCAGAGTACACA
CCATCGTTATTTCAACTCAACACGACGAAAAAGTCAXXXXXXXXXXACCATTTGAATCCA
TCAGGTCGTTTNGTCATTGGTGGCCCAANGGGTGATTNTGGTTTAACCGGTCGTAANATC
ATCATTGATTCANATGGTGGNTGGGGNGCNCNCGGTGGTGGNGCNTTNTCTGGTAAAAAT
GCCNCTAAAGTCGATCGTTNTGGNGCNTACGCTGCTAAANGGATCGCTAAATCTTTAGNT
GCCNCTGGTCTTNCCGATCGTTGTTTAGNTCAAGTTTCANACTCTATTGGTGNTGCCNAN
CCNCTCAGNGTNTTTGTTNANACCTATGGGACCGGTAAAAANACTGATGCTGAAANCCNT
GCCATCATCGATAANAACTTTGATTTACGNCCAGGTTGTTTAANG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
sf*VIFHTIYKMSSYLLTSESVTEGHPDKICDQVSDAVLDACLAQDPLSKVACETATKTG
MVMILGEITTKANVDYQKVVREAVKKIGYDDSSKGFDYKTCNVLVAIEQQSPDIAQGVHI
GKVNPEDIGAGDQGHMFGYATNETPTLMPLTHFLASELVNKLTELRHNGTLAWARPDAKT
QVTVEYKKEGHKITPVRVHTIVISTQHDEKV---

---HLNPSGRXVIGGPXGDXGLTGRXIIIDSXGGWGAXGGGAXSGKNAXKVDRXGAYAAK
XIAKSLXAXGLXDRCLXQVSXSIGXAXPLXVFVXTYGTGKXTDAEXXAIIDXNFDLRPGC
LX
Translated Amino Acid sequence (All Frames) 
Frame A:
vvfk*yftqyikchliy*lvnpspkviqiksvikyqmlfsmlv*lkihyqkllvklqpkl
vwl*f*vklppkptltikklfvkllkrlvmmthqkvlitklamf*lqlnnnhqillkvst
lvkltqkilvlvikvtclvmpptkpql*chllisspln*stn*lnyvtmvp*pglvqmpr
lksllntkrkvtkslqseytpslfqlnttkks---

---ti*ihqvvxslvaqxvixv*pvvxsslixmvxgxxxvvxxxlvkmplksivxxxtll
xgslnl*xplvxpivv*xkfxtllvxpxxsxxlxxpmgpvkxlmlkxxpssixtliyxqv
v*

Frame B:
*flsnishni*nvilfin**irhrrssr*nl*ssirccsrclfssrsiikscl*ncnqnw
ygydfr*nyhqsqr*lskscs*sc*kdwl**likrf*lqnlqcfscn*ttitrycsrcph
w*s*prrywcw*srshvwlchqrnpnfnatysfprl*isqqin*itsqwylslgssrcqd
sshc*iqkgrsqnhssqsthhryfnstrrks---

---pfesirsfxhwwpxg*xwfnrs*xhh*fxwwxgxxrwwxxxw*kcx*srsxwxxrc*
xdr*ifxcxwsxrslfxssfxlywxcxxxqxxcxxlwdr*kx*c*xpchhr*xl*ftxrl
fx

Frame C:
sf*VIFHTIYKMSSYLLTSESVTEGHPDKICDQVSDAVLDACLAQDPLSKVACETATKTG
MVMILGEITTKANVDYQKVVREAVKKIGYDDSSKGFDYKTCNVLVAIEQQSPDIAQGVHI
GKVNPEDIGAGDQGHMFGYATNETPTLMPLTHFLASELVNKLTELRHNGTLAWARPDAKT
QVTVEYKKEGHKITPVRVHTIVISTQHDEKV---

---HLNPSGRXVIGGPXGDXGLTGRXIIIDSXGGWGAXGGGAXSGKNAXKVDRXGAYAAK
XIAKSLXAXGLXDRCLXQVSXSIGXAXPLXVFVXTYGTGKXTDAEXXAIIDXNFDLRPGC
LX
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA896 (VFA896Q) /CSM/VF/VFA8-D/VFA896Q.Seq.d/                       1721   0.0
VFN312 (VFN312Q) /CSM/VF/VFN3-A/VFN312Q.Seq.d/                       1233   0.0
VFN128 (VFN128Q) /CSM/VF/VFN1-B/VFN128Q.Seq.d/                       1233   0.0
VFM766 (VFM766Q) /CSM/VF/VFM7-C/VFM766Q.Seq.d/                       1233   0.0
VFL503 (VFL503Q) /CSM/VF/VFL5-A/VFL503Q.Seq.d/                       1233   0.0
VFL319 (VFL319Q) /CSM/VF/VFL3-A/VFL319Q.Seq.d/                       1233   0.0
VFL166 (VFL166Q) /CSM/VF/VFL1-C/VFL166Q.Seq.d/                       1233   0.0
VFI164 (VFI164Q) /CSM/VF/VFI1-C/VFI164Q.Seq.d/                       1233   0.0
VFI116 (VFI116Q) /CSM/VF/VFI1-A/VFI116Q.Seq.d/                       1233   0.0
VFF889 (VFF889Q) /CSM/VF/VFF8-D/VFF889Q.Seq.d/                       1233   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AJ390497|AJ390497.1 Candida albicans partial mRNA for S-adenosylmethionine synthetase 2  (sam2 gene).   107  2e-50  3
AX595308|AX595308.1 Sequence 962 from Patent EP1258494.          109  3e-39  4
M23368|M23368.1 Saccharomyces cerevisiae S-adenosylmethionine synthetase (SAM2) gene, complete cds.   109  1e-38  4
U33057|U33057.1 Saccharomyces cerevisiae chromosome IV cosmids 8166, 9787, 9717, and lambda 3073.   109  6e-34  4
CA754635| (NM_068470) S-adenosylmethionine synthetase [Caenorhabditis, mRNA sequence.    66  3e-31  5
T37377|T37377.1 EST102487 S. cerevisiae strain X2180-1A Saccharomyces cerevisiae cDNA 5' end, mRNA sequence.    70  9e-27  3
AL424144|AL424144.1 T7 end of clone AZ0AA012F03 of library AZ0AA from strain CBS 712 of Kluyveromyces marxianus.    78  8e-25  3
BU256602|BU256602.1 603748494F1 CSEQCHN38 Gallus gallus cDNA clone ChEST657k23 5', mRNA sequence.    76  9e-24  4
AU135597|AU135597.1 Homo sapiens cDNA clone:PLACE1002456, 5' end, expressed in placenta.    78  1e-23  4
AR351235|AR351235.1 Sequence 278 from patent US 6586572.          78  2e-23  4
dna update 2003. 8.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54F07) RecName: Full=S-adenosylmethionine synthetase;         ...   403   e-143
AY324626_1(AY324626|pid:none) Amoeba proteus S-adenosylmethionin...   291   e-103
AM177232_1(AM177232|pid:none) Oryza sativa partial sams gene for...   282   5e-98
AM177301_1(AM177301|pid:none) Oryza rufipogon partial sams gene ...   282   5e-98
AM177307_1(AM177307|pid:none) Oryza officinalis partial sams gen...   282   6e-98
(A2Y053) RecName: Full=S-adenosylmethionine synthetase 1;       ...   281   8e-98
BT088567_1(BT088567|pid:none) Zea mays full-length cDNA clone ZM...   283   1e-97
(Q9FUZ1) RecName: Full=S-adenosylmethionine synthetase 2;       ...   285   1e-97
AM177274_1(AM177274|pid:none) Oryza rufipogon partial sams gene ...   280   1e-97
AM177290_1(AM177290|pid:none) Oryza rufipogon partial sams gene ...   280   1e-97
protein update 2009. 6.17
PSORT 

 psg: 0.87  gvh: 0.58  alm: 0.43  top: 0.37  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 20.0 %: extracellular, including cell wall
	 16.0 %: mitochondrial
	 16.0 %: cytoplasmic
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFA896 is nuc
5' end seq. ID VFA896F
5' end seq. 
>VFA896F.Seq
GTAGTTTTTAAGTAATATTTCACACAATATATAAAATGTCATCTTATTTATTAACTAGTG
AATCCGTCACCGAAGGTCATCCAGATAAAATCTGTGATCAAGTATCAGATGCTGTTCTCG
ATGCTTGTTTAGCTCAAGATCCATTATCAAAAGTTGCTTGTGAAACTGCAACCAAAACTG
GTATGGTTATGATTTTAGGTGAAATTACCACCAAAGCCAACGTTGACTATCAAAAAGTTG
TTCGTGAAGCTGTTAAAAAGATTGGTTATGATGACTCATCAAAAGGTTTTGATTACAAAA
CTTGCAATGTTTTAGTTGCAATTGAACAACAATCACCAGATATTGCTCAAGGTGTCCACA
TTGGTAAAGTTAACCCAGAAGATATTGGTGCTGGTGATCAAGGTCACATGTTTGGTTATG
CCACCAACGAAACCCCAACTTTAATGCCACTTACTCATTTCCTCGCCTCTGAATTAGTCA
ACAAATTAACTGAATTACGTCACAATGGTACCTTAGCCTGGGCTCGTCCAGATGCCAAGA
CTCAAGTCACTGTTGAATACAAAAAGGAAGGTCACAAAATCACTCCAGTCAGAGTACACA
CCATCGTTATTTCAACTCAACACGACGAAAAAGTCA----------
Length of 5' end seq. 636
3' end seq. ID VFA896Z
3' end seq. 
>VFA896Z.Seq
----------ACCATTTGAATCCATCAGGTCGTTTNGTCATTGGTGGCCCAANGGGTGAT
TNTGGTTTAACCGGTCGTAANATCATCATTGATTCANATGGTGGNTGGGGNGCNCNCGGT
GGTGGNGCNTTNTCTGGTAAAAATGCCNCTAAAGTCGATCGTTNTGGNGCNTACGCTGCT
AAANGGATCGCTAAATCTTTAGNTGCCNCTGGTCTTNCCGATCGTTGTTTAGNTCAAGTT
TCANACTCTATTGGTGNTGCCNANCCNCTCAGNGTNTTTGTTNANACCTATGGGACCGGT
AAAAANACTGATGCTGAAANCCNTGCCATCATCGATAANAACTTTGATTTACGNCCAGGT
TGTTTAANG
Length of 3' end seq. 359
Connected seq. ID VFA896P
Connected seq. 
>VFA896P.Seq
GTAGTTTTTAAGTAATATTTCACACAATATATAAAATGTCATCTTATTTATTAACTAGTG
AATCCGTCACCGAAGGTCATCCAGATAAAATCTGTGATCAAGTATCAGATGCTGTTCTCG
ATGCTTGTTTAGCTCAAGATCCATTATCAAAAGTTGCTTGTGAAACTGCAACCAAAACTG
GTATGGTTATGATTTTAGGTGAAATTACCACCAAAGCCAACGTTGACTATCAAAAAGTTG
TTCGTGAAGCTGTTAAAAAGATTGGTTATGATGACTCATCAAAAGGTTTTGATTACAAAA
CTTGCAATGTTTTAGTTGCAATTGAACAACAATCACCAGATATTGCTCAAGGTGTCCACA
TTGGTAAAGTTAACCCAGAAGATATTGGTGCTGGTGATCAAGGTCACATGTTTGGTTATG
CCACCAACGAAACCCCAACTTTAATGCCACTTACTCATTTCCTCGCCTCTGAATTAGTCA
ACAAATTAACTGAATTACGTCACAATGGTACCTTAGCCTGGGCTCGTCCAGATGCCAAGA
CTCAAGTCACTGTTGAATACAAAAAGGAAGGTCACAAAATCACTCCAGTCAGAGTACACA
CCATCGTTATTTCAACTCAACACGACGAAAAAGTCA----------ACCATTTGAATCCA
TCAGGTCGTTTNGTCATTGGTGGCCCAANGGGTGATTNTGGTTTAACCGGTCGTAANATC
ATCATTGATTCANATGGTGGNTGGGGNGCNCNCGGTGGTGGNGCNTTNTCTGGTAAAAAT
GCCNCTAAAGTCGATCGTTNTGGNGCNTACGCTGCTAAANGGATCGCTAAATCTTTAGNT
GCCNCTGGTCTTNCCGATCGTTGTTTAGNTCAAGTTTCANACTCTATTGGTGNTGCCNAN
CCNCTCAGNGTNTTTGTTNANACCTATGGGACCGGTAAAAANACTGATGCTGAAANCCNT
GCCATCATCGATAANAACTTTGATTTACGNCCAGGTTGTTTAANG
Length of connected seq. 995
Full length Seq ID -
Full length Seq. -
Length of full length seq. -