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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFF163
Library VF
(Link to library)
Clone ID VFF163
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16605-1
Original site URL
Representative seq. ID VFF163P
(Link to Original site)
Representative DNA sequence 
>VFF163 (VFF163Q) /CSM/VF/VFF1-C/VFF163Q.Seq.d/
ATNNATTAAATATATATATACTAAAAATGAGAGTTTTATCATTCCTTTGTTTATTATTAG
TTAGCTACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCACCA
ACTGGATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATCAAA
TCTTCAAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCGTTT
TGGGTTTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGGGTA
CCCCATTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCCCAG
CCCCAACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTCAAT
GTGGTGGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTGCAT
CTGGAACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAAAAT
CATACGGTAACAATGGTTGXXXXXXXXXXTGTTGCAATTGATGCTTCAAATGAATCNTTC
CAATTATATGAATCAGGTATCTACTATGAACCAGCATGTTCTCCAACCCAACTTGATCAT
GGTGTTTTAGTTGTTGGTTATGGTTCAGGTTCAAGTTCATCATCTGGTTCATCATCTGGT
AAATCATCATCATCATCATCAACTGGTGGTAAAACTTCATCCTCATCATCATCAGGTAAA
GCTTCATCATCATCATCAGGCAAAGNTTCATCATCATCATCATCAGGTAAAACTTCATCT
GCTGCTTCATCAACCTCTGGTTCTCAATCAGGTTCCCAATCAGGTAGCCAATCAGGCCAA
TCCACCGGTTCACAATCAGGTCAAACCTCTGCTTCTGGTCAAGCATCAGCATCAGGTTCT
GGTTCTGGCTCAGGTTCAGGTTCAGGTTCAGGTTCAGGCTCAGGTGCTGTTNAGGCCTCA
TCTGGTAACNANTGGATCGTTAAAAACTCATNGGGTNCTNNATGGA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
XLNIYILKMRVLSFLCLLLVSYASAKQQFSELQYRNAFTNWMQAHQRTYSSEEFNARYQI
FKSNMDYVHQWNSKGGETVLGLNVFADITNQEYRTTYLGTPFDGSALIGTEEEKIFSTPA
PTVDWRAQGAVTPIKNQGQCGGCWSFSTTGSTEGAHFIASGTKKDLVSLSEQNLIDCSKS
YGNNG---

---VAIDASNESFQLYESGIYYEPACSPTQLDHGVLVVGYGSGSSSSSGSSSGKSSSSSS
TGGKTSSSSSSGKASSSSSGKXSSSSSSGKTSSAASSTSGSQSGSQSGSQSGQSTGSQSG
QTSASGQASASGSGSGSGSGSGSGSGSGAVXASSGNXWIVKNSXGXXW
Translated Amino Acid sequence (All Frames) 
Frame A:
xx*iyiy*k*efyhsfvyy*latlllnnnslnyntemlsptgckltkeliplknlmlvik
ssnpiwimytngiqkvvkpfwv*mfslilptknielptwvphsmvqpslvlkkrkssppq
pqlligelkvlshqlkikvnvvaaghsqplvqlkvltllhleqkki*fhylnkt*sivqn
htvtmv---

---ccn*cfk*ixpii*iryll*tsmfsnpt*swcfscwlwfrfkfiiwfiiw*iiiiii
nww*nfiliiir*sfiiiirqxfiiiiir*nficcfinlwfsirfpir*pirpihrftir
snlcfwssisirfwfwlrfrfrfrfrlrccxgliw*xxdr*klxgxxm

Frame B:
xikyiytknesfiiplfiis*lrfc*ttil*itiqkcfhqldasspknlfl*ri*cslsn
lqiqyglctpmefkrw*nrfgfkcfr*yyqpri*nyllgypirwfsphwy*rrenllhps
pnc*lessrcchtn*ksrsmwwllvilnhwfn*rcslyciwnkkrfsfii*tkldrlfki
ir*qwl---

---VAIDASNESFQLYESGIYYEPACSPTQLDHGVLVVGYGSGSSSSSGSSSGKSSSSSS
TGGKTSSSSSSGKASSSSSGKXSSSSSSGKTSSAASSTSGSQSGSQSGSQSGQSTGSQSG
QTSASGQASASGSGSGSGSGSGSGSGSGAVXASSGNXWIVKNSXGXXW

Frame C:
XLNIYILKMRVLSFLCLLLVSYASAKQQFSELQYRNAFTNWMQAHQRTYSSEEFNARYQI
FKSNMDYVHQWNSKGGETVLGLNVFADITNQEYRTTYLGTPFDGSALIGTEEEKIFSTPA
PTVDWRAQGAVTPIKNQGQCGGCWSFSTTGSTEGAHFIASGTKKDLVSLSEQNLIDCSKS
YGNNG---

---lqlmlqmnxsnymnqvstmnqhvlqpnlimvf*llvmvqvqvhhlvhhlvnhhhhhq
lvvklhphhhqvklhhhhqakxhhhhhqvklhlllhqplvlnqvpnqvanqanppvhnqv
kplllvkhqhqvlvlaqvqvqvqvqaqvlxrphlvtxgslkthxvlxg
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO623 (VFO623Q) /CSM/VF/VFO6-A/VFO623Q.Seq.d/                       1059   0.0
VFO306 (VFO306Q) /CSM/VF/VFO3-A/VFO306Q.Seq.d/                       1059   0.0
VFN628 (VFN628Q) /CSM/VF/VFN6-B/VFN628Q.Seq.d/                       1059   0.0
VFN294 (VFN294Q) /CSM/VF/VFN2-D/VFN294Q.Seq.d/                       1059   0.0
VFM872 (VFM872Q) /CSM/VF/VFM8-C/VFM872Q.Seq.d/                       1059   0.0
VFM804 (VFM804Q) /CSM/VF/VFM8-A/VFM804Q.Seq.d/                       1059   0.0
VFM544 (VFM544Q) /CSM/VF/VFM5-B/VFM544Q.Seq.d/                       1059   0.0
VFM320 (VFM320Q) /CSM/VF/VFM3-A/VFM320Q.Seq.d/                       1059   0.0
VFM263 (VFM263Q) /CSM/VF/VFM2-C/VFM263Q.Seq.d/                       1059   0.0
VFL463 (VFL463Q) /CSM/VF/VFL4-C/VFL463Q.Seq.d/                       1059   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

L36204|L36204.1 Dictyostelium discoideum cysteine proteinase (CP4) mRNA, complete cds.   922  0.0  5
L36205|L36205.1 Dictyostelium discoideum cysteine proteinase CP5 mRNA, complete cds.   121  e-107  9
U72746|U72746.1 Dictyostelium discoideum cysteine proteinase (cprG) mRNA, complete cds.   131  4e-98  7
AC117072|AC117072.2 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.   121  1e-94  10
U72745|U72745.1 Dictyostelium discoideum cysteine proteinase (cprF) mRNA, complete cds.   115  7e-90  6
X03344|X03344.1 Dictyostelium discoideum mRNA for cysteine proteinase 2.    74  1e-39  7
M16039|M16039.1 Dictyostelium discoideum pst-cath gene encoding pst-cathepsin, complete cds.    74  6e-37  9
X02407|X02407.1 D.discoideum mRNA for cysteine proteinase 1.      70  9e-19  3
AJ517568|AJ517568.1 Dreissena polymorpha EST, clone atrado65.     68  2e-07  1
BJ171907|BJ171907.1 Physcomitrella patens subsp. patens cDNA clone:pph30i20, 3' end, single read.    66  1e-06  1
dna update 2003. 9.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

L36204_1(L36204|pid:none) Dictyostelium discoideum cysteine prot...   367   e-100
(Q94504) RecName: Full=Cysteine proteinase 7;          EC=3.4.22...   281   2e-74
(P54640) RecName: Full=Cysteine proteinase 5;          EC=3.4.22...   273   6e-72
(Q94503) RecName: Full=Cysteine proteinase 6;          EC=3.4.22...   272   1e-71
L36205_1(L36205|pid:none) Dictyostelium discoideum cysteine prot...   267   5e-70
(P04989) RecName: Full=Cysteine proteinase 2;          EC=3.4.22...   195   2e-48
EF053509_1(EF053509|pid:none) Acanthamoeba castellanii cysteine ...   163   8e-39
AC117076_20(AC117076|pid:none) Dictyostelium discoideum chromoso...   152   1e-35
AK226753_1(AK226753|pid:none) Arabidopsis thaliana mRNA for papa...   137   8e-31
AC000132_24(AC000132|pid:none) Sequence of BAC F21M12 from Arabi...   137   8e-31
protein update 2009. 6.19
PSORT 

 psg: 0.96  gvh: 0.87  alm: 0.52  top: 0.40  tms: 0.00  mit: 0.37  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	 12.0 %: mitochondrial
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: Golgi

>> prediction for VFF163 is exc
5' end seq. ID VFF163F
5' end seq. 
>VFF163F.Seq
ATNNATTAAATATATATATACTAAAAATGAGAGTTTTATCATTCCTTTGTTTATTATTAG
TTAGCTACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCACCA
ACTGGATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATCAAA
TCTTCAAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCGTTT
TGGGTTTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGGGTA
CCCCATTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCCCAG
CCCCAACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTCAAT
GTGGTGGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTGCAT
CTGGAACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAAAAT
CATACGGTAACAATGGTTG----------
Length of 5' end seq. 559
3' end seq. ID VFF163Z
3' end seq. 
>VFF163Z.Seq
----------TGTTGCAATTGATGCTTCAAATGAATCNTTCCAATTATATGAATCAGGTA
TCTACTATGAACCAGCATGTTCTCCAACCCAACTTGATCATGGTGTTTTAGTTGTTGGTT
ATGGTTCAGGTTCAAGTTCATCATCTGGTTCATCATCTGGTAAATCATCATCATCATCAT
CAACTGGTGGTAAAACTTCATCCTCATCATCATCAGGTAAAGCTTCATCATCATCATCAG
GCAAAGNTTCATCATCATCATCATCAGGTAAAACTTCATCTGCTGCTTCATCAACCTCTG
GTTCTCAATCAGGTTCCCAATCAGGTAGCCAATCAGGCCAATCCACCGGTTCACAATCAG
GTCAAACCTCTGCTTCTGGTCAAGCATCAGCATCAGGTTCTGGTTCTGGCTCAGGTTCAG
GTTCAGGTTCAGGTTCAGGCTCAGGTGCTGTTNAGGCCTCATCTGGTAACNANTGGATCG
TTAAAAACTCATNGGGTNCTNNATGGA
Length of 3' end seq. 497
Connected seq. ID VFF163P
Connected seq. 
>VFF163P.Seq
ATNNATTAAATATATATATACTAAAAATGAGAGTTTTATCATTCCTTTGTTTATTATTAG
TTAGCTACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCACCA
ACTGGATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATCAAA
TCTTCAAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCGTTT
TGGGTTTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGGGTA
CCCCATTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCCCAG
CCCCAACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTCAAT
GTGGTGGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTGCAT
CTGGAACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAAAAT
CATACGGTAACAATGGTTG----------TGTTGCAATTGATGCTTCAAATGAATCNTTC
CAATTATATGAATCAGGTATCTACTATGAACCAGCATGTTCTCCAACCCAACTTGATCAT
GGTGTTTTAGTTGTTGGTTATGGTTCAGGTTCAAGTTCATCATCTGGTTCATCATCTGGT
AAATCATCATCATCATCATCAACTGGTGGTAAAACTTCATCCTCATCATCATCAGGTAAA
GCTTCATCATCATCATCAGGCAAAGNTTCATCATCATCATCATCAGGTAAAACTTCATCT
GCTGCTTCATCAACCTCTGGTTCTCAATCAGGTTCCCAATCAGGTAGCCAATCAGGCCAA
TCCACCGGTTCACAATCAGGTCAAACCTCTGCTTCTGGTCAAGCATCAGCATCAGGTTCT
GGTTCTGGCTCAGGTTCAGGTTCAGGTTCAGGTTCAGGCTCAGGTGCTGTTNAGGCCTCA
TCTGGTAACNANTGGATCGTTAAAAACTCATNGGGTNCTNNATGGA
Length of connected seq. 1056
Full length Seq ID -
Full length Seq. -
Length of full length seq. -