>VFF543 (VFF543Q) /CSM/VF/VFF5-B/VFF543Q.Seq.d/
TTAAAAAATCTGATGAATATAANGCANTNGCAAATAANCATTTTAGNGANCAAAAATNTG
ATTTAGCAGCAGANGTTTNTNCAAANGCANTTAAATNTCATCCANCAGCAATTTTNTNTT
CAAATAGATCATTTTCAAATTTTAAGAATGAGTTNTNTGTAAANGCATTNCANGANGCTC
AANCCTNTCATGAAANGGATCCAACTTACATAAAAGCATNTTATCGTTTAGGTXXXXXXX
XXXTGGATAGGGGTAGTTTTAGTTTTGAAATTATCATCACTCTATTGGCTTATAAATTAT
TGTACCCAAATCATATGCATTTGACAAGGGGTAACCATGAGACAACCGATATGAATCGTT
TCTATGGTTTCCAAGGCGAAGTGGTTGCAAAATACAGTGAGATGGTATTCGATCTATTCT
CAGAGTTGTTCAATTGGTTCCCATTGGCATTCGTGTTGGATGAATCGTTTATGGTGGTAC
ATGGTGGTTTGTTTGGTAAGGATGGTGTCACACTTGACGATATTAGAAAGATTAAACGTA
ATGCACCAGATCCAAAAGATAATGAATTGGTTCAATGCTTACTTTGGTCTGATCCTCAAT
CAAATCCTGGTATTGCTCCTTCTTCAAGAGGTGTAGGTGTTTACTTTGGACCTGATGTCA
CCAGACGTTTCCTCAAGGAAAATAATCTATCAACTATCATTAGATCTCATGAAGTTAAAG
AAAAAGGTTATCAAATCGATGATGATGGTTCTCTCATCACTGTTTTCTCTGCTCCAAATT
ATTGTGATCAATCTGGTAATCTTGGCTCATTTATAAATATAACGAAGATAAAATTAAAAT
TACAACTTTGAAGCTGTNAACATCCGAATAACCACCAAGCATAGCCAAAA

KKSDEYXAXANXHFXXQKXDLAAXVXXXAXKXHPXAIXXSNRSFSNFKNEXXVXAXXXAQ
XXHEXDPTYIKAXYRLG---

---DRGSFSFEIIITLLAYKLLYPNHMHLTRGNHETTDMNRFYGFQGEVVAKYSEMVFDL
FSELFNWFPLAFVLDESFMVVHGGLFGKDGVTLDDIRKIKRNAPDPKDNELVQCLLWSDP
QSNPGIAPSSRGVGVYFGPDVTRRFLKENNLSTIIRSHEVKEKGYQIDDDGSLITVFSAP
NYCDQSGNLGSFINITKIKLKLQL*scxhpnnhqa*pk

Frame A:
lknlmnixxxqixilxxknxi*qqxfxqxxlnxixqqfxxqidhfqilrmsxx*xhxxxl
xpxmkxiqlt*khxiv*---

---wigvvlvlklsslywlinyctqiici*qgvtmrqpi*ivsmvskakwlqntvrwysi
ysqscsigshwhscwmnrlwwymvvclvrmvshltilerlnvmhqiqkimnwfnayfgli
lnqilvllllqev*vftldlmspdvssrkiiyqlsldlmklkkkviksmmmvlsslfsll
qiivinlvilahl*i*rr*n*nynfeavnirittkhsq

Frame B:
*ki**i*xxxk*xf*xxkx*fssrxxxkxx*xssxsnfxfk*iifkf*e*vxckxixxxs
xls*xgsnlhksxlsfr---

---g*g*f*f*nyhhsigl*iivpksyafdkg*p*dnryesflwfprrsgckiq*dgirs
ilrvvqlvpigirvg*ivyggtwwfvw*gwcht*ry*kd*t*ctrskr**igsmltlv*s
sikswycsffkrcrcllwt*chqtfpqgk*sinyh*is*s*rkrlsnr**wfshhcflcs
kll*siw*swliykynedkikittlklxtse*ppsiak

Frame C:
KKSDEYXAXANXHFXXQKXDLAAXVXXXAXKXHPXAIXXSNRSFSNFKNEXXVXAXXXAQ
XXHEXDPTYIKAXYRLG---

---DRGSFSFEIIITLLAYKLLYPNHMHLTRGNHETTDMNRFYGFQGEVVAKYSEMVFDL
FSELFNWFPLAFVLDESFMVVHGGLFGKDGVTLDDIRKIKRNAPDPKDNELVQCLLWSDP
QSNPGIAPSSRGVGVYFGPDVTRRFLKENNLSTIIRSHEVKEKGYQIDDDGSLITVFSAP
NYCDQSGNLGSFINITKIKLKLQL*scxhpnnhqa*pk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF543 (VFF543Q) /CSM/VF/VFF5-B/VFF543Q.Seq.d/                       1503   0.0
CFG219 (CFG219Q) /CSM/CF/CFG2-A/CFG219Q.Seq.d/                       1247   0.0
AFO461 (AFO461Q) /CSM/AF/AFO4-C/AFO461Q.Seq.d/                       1235   0.0
AFL662 (AFL662Q) /CSM/AF/AFL6-C/AFL662Q.Seq.d/                       1235   0.0
SFD165 (SFD165Q) /CSM/SF/SFD1-C/SFD165Q.Seq.d/                       1227   0.0
VFI734 (VFI734Q) /CSM/VF/VFI7-B/VFI734Q.Seq.d/                       1225   0.0
VFF491 (VFF491Q) /CSM/VF/VFF4-D/VFF491Q.Seq.d/                       1225   0.0
SFG624 (SFG624Q) /CSM/SF/SFG6-A/SFG624Q.Seq.d/                       1225   0.0
VFK254 (VFK254Q) /CSM/VF/VFK2-C/VFK254Q.Seq.d/                       1209   0.0
AFA889 (AFA889Q) /CSM/AF/AFA8-D/AFA889Q.Seq.d/                       1203   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BU041737|BU041737.1 PP_LEa0010F03f Peach developing fruit mesocarp Prunus persica cDNA clone PP_LEa0010F03f, mRNA sequence.    60  5e-05  1
CB821514|CB821514.1 EST 2365 Half-Ripe Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone be002m12 3', mRNA sequence.    60  5e-05  1
CA933944|CA933944.1 MTU3TS.P14.F11 Aspen stem cDNA Library Populus tremuloides cDNA, mRNA sequence.    54  6e-05  2
BF272976|BF272976.1 GA__Eb0016H03f Gossypium arboreum 7-10 dpa fiber library Gossypium arboreum cDNA clone GA__Eb0016H03f, mRNA sequence.    48  7e-05  2
BQ916199|BQ916199.1 QHB17B15.yg.ab1 QH_ABCDI sunflower RHA801 Helianthus annuus cDNA clone QHB17B15, mRNA sequence.    58  2e-04  1
AJ561748|AJ561748.1 Cryptosporidium parvum GSS, PAC clone pica_0004_g07, T7 end  sequence.    34  0.002  3
BU822525|BU822525.1 UB38BPH04 Populus tremula cambium cDNA library Populus tremula cDNA 5 prime, mRNA sequence.    54  0.003  1
BU895987|BU895987.1 X033H08 Populus wood cDNA library Populus tremula x Populus tremuloides cDNA 5 prime, mRNA sequence.    54  0.003  1
AV202542|AV202542.1 Caenorhabditis elegans cDNA clone:yk551g8 : 5' end, single read.    44  0.009  2
C68648|C68648.1 C.elegans cDNA clone yk306a6 : 5' end, single read.    44  0.009  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR848198_1(CR848198|pid:none) Xenopus tropicalis finished cDNA, ...   244   4e-63
BC080162_1(BC080162|pid:none) Xenopus tropicalis ppp5c protein, ...   244   4e-63
CR859667_1(CR859667|pid:none) Pongo abelii mRNA; cDNA DKFZp459E1...   243   5e-63
(P53041) RecName: Full=Serine/threonine-protein phosphatase 5;  ...   243   6e-63
AK297095_1(AK297095|pid:none) Homo sapiens cDNA FLJ55717 complet...   243   6e-63
AK223222_1(AK223222|pid:none) Homo sapiens mRNA for protein phos...   243   6e-63
AK293402_1(AK293402|pid:none) Homo sapiens cDNA FLJ55048 complet...   243   6e-63
BC000750_1(BC000750|pid:none) Homo sapiens protein phosphatase 5...   242   1e-62
BC078786_1(BC078786|pid:none) Rattus norvegicus protein phosphat...   241   2e-62
(Q60676) RecName: Full=Serine/threonine-protein phosphatase 5;  ...   241   2e-62

 psg: 0.74  gvh: 0.42  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VFF543 is cyt

>VFF543F.Seq
TTAAAAAATCTGATGAATATAANGCANTNGCAAATAANCATTTTAGNGANCAAAAATNTG
ATTTAGCAGCAGANGTTTNTNCAAANGCANTTAAATNTCATCCANCAGCAATTTTNTNTT
CAAATAGATCATTTTCAAATTTTAAGAATGAGTTNTNTGTAAANGCATTNCANGANGCTC
AANCCTNTCATGAAANGGATCCAACTTACATAAAAGCATNTTATCGTTTAGGT-------
---

>VFF543Z.Seq
----------TGGATAGGGGTAGTTTTAGTTTTGAAATTATCATCACTCTATTGGCTTAT
AAATTATTGTACCCAAATCATATGCATTTGACAAGGGGTAACCATGAGACAACCGATATG
AATCGTTTCTATGGTTTCCAAGGCGAAGTGGTTGCAAAATACAGTGAGATGGTATTCGAT
CTATTCTCAGAGTTGTTCAATTGGTTCCCATTGGCATTCGTGTTGGATGAATCGTTTATG
GTGGTACATGGTGGTTTGTTTGGTAAGGATGGTGTCACACTTGACGATATTAGAAAGATT
AAACGTAATGCACCAGATCCAAAAGATAATGAATTGGTTCAATGCTTACTTTGGTCTGAT
CCTCAATCAAATCCTGGTATTGCTCCTTCTTCAAGAGGTGTAGGTGTTTACTTTGGACCT
GATGTCACCAGACGTTTCCTCAAGGAAAATAATCTATCAACTATCATTAGATCTCATGAA
GTTAAAGAAAAAGGTTATCAAATCGATGATGATGGTTCTCTCATCACTGTTTTCTCTGCT
CCAAATTATTGTGATCAATCTGGTAATCTTGGCTCATTTATAAATATAACGAAGATAAAA
TTAAAATTACAACTTTGAAGCTGTNAACATCCGAATAACCACCAAGCATAGCCAAAA

>VFF543P.Seq
TTAAAAAATCTGATGAATATAANGCANTNGCAAATAANCATTTTAGNGANCAAAAATNTG
ATTTAGCAGCAGANGTTTNTNCAAANGCANTTAAATNTCATCCANCAGCAATTTTNTNTT
CAAATAGATCATTTTCAAATTTTAAGAATGAGTTNTNTGTAAANGCATTNCANGANGCTC
AANCCTNTCATGAAANGGATCCAACTTACATAAAAGCATNTTATCGTTTAGGT-------
---TGGATAGGGGTAGTTTTAGTTTTGAAATTATCATCACTCTATTGGCTTATAAATTAT
TGTACCCAAATCATATGCATTTGACAAGGGGTAACCATGAGACAACCGATATGAATCGTT
TCTATGGTTTCCAAGGCGAAGTGGTTGCAAAATACAGTGAGATGGTATTCGATCTATTCT
CAGAGTTGTTCAATTGGTTCCCATTGGCATTCGTGTTGGATGAATCGTTTATGGTGGTAC
ATGGTGGTTTGTTTGGTAAGGATGGTGTCACACTTGACGATATTAGAAAGATTAAACGTA
ATGCACCAGATCCAAAAGATAATGAATTGGTTCAATGCTTACTTTGGTCTGATCCTCAAT
CAAATCCTGGTATTGCTCCTTCTTCAAGAGGTGTAGGTGTTTACTTTGGACCTGATGTCA
CCAGACGTTTCCTCAAGGAAAATAATCTATCAACTATCATTAGATCTCATGAAGTTAAAG
AAAAAGGTTATCAAATCGATGATGATGGTTCTCTCATCACTGTTTTCTCTGCTCCAAATT
ATTGTGATCAATCTGGTAATCTTGGCTCATTTATAAATATAACGAAGATAAAATTAAAAT
TACAACTTTGAAGCTGTNAACATCCGAATAACCACCAAGCATAGCCAAAA