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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFJ332
Library VF
(Link to library)
Clone ID VFJ332
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U09061-1|Contig-U10621-1
Original site URL
Representative seq. ID VFJ332P
(Link to Original site)
Representative DNA sequence 
>VFJ332 (VFJ332Q) /CSM/VF/VFJ3-B/VFJ332Q.Seq.d/
TTTAGTGTAAAATAAATTAAAAAAATGGAAAAAAAGATACTTTTATTTCTTTTAATTCTA
TCCATTTCATTTAATAATTATTTTGTCTCATCAACATCATCGTCATCATCATCATCAAGT
GAATGCCATCAAAAGATTTTTAGCGTTTCAGAAGTTAACCCATACAATATTAATAGTTTA
GTTGTTATTCAAGGTATTTTTTGTTTAAATAGTACAGAAGGAATTAAAGTTTCACTCAAT
GTACCAGAAGAAGGATCAAATATTTCATATGGATTAGATTGTGATGATAGTTTAACAATT
GATTCAATTGGTGAAACCATTACATGTAGATTATCAGATAATTATAGTGGTAAAAATGCA
GTAAACTTTACAACATTGGCAATTGTAATGGTTTATACACCAATTCCAGTACCAACACCA
ACACCATCAAAAACTCCAACAAAACAAATTGCTGTTGGACCAGTACCAGTATTAGGAACT
AGTAAATATATATTCACAAACAGTAGTTGTCCXXXXXXXXXXGGTACAACCTATACAAAT
GGTAAACCATCTTCAAAACCATGGCTTAACCAATTGCAATATGATGTTGGCTTCACTTCA
GTTGAGGAACCATCATTTAGCTATGAAAATTACAAAGGTGCCTATTTACCATTATCATCA
TCAGCAGCCACAATTACTTTAACTCCAACTATTAATATCAACTATTTACCAACTGTTTAT
TATACAACTAGTGGTGGTGCAACTTATTCAATGATATTTAAAATATCGAATCAACATGAC
TCAATGAGTGGATATTCAAATGTTTTCTCTGAAACTTCAAATTCAATCACATTATCAAGT
GGAGATGGTAAAAACTCTTTAATGGTTATTAGTTTATCTGATATCTATAGTTTTGATCAA
ACAACCGCACCAAATCATGCTTATCTTTCATTGCTACCAAATCCAGGTTCTTCTTTAATA
AGCAATTATGATTCAGATTCTTTATATGTTTCAGTTAATATACCATCAATCTATAGTACA
AACAATCAAAATCCATATACAATTCAATTGAATATCAATGTTTATGATATTGATACATCA
TCTCATTCTAAACTTCCAAAATGGGCTCTTGGTCTAATTGTAGGTTTCTCTGTATTCATC
GGTATTGTAATTATTTTCATTATAGCTAAATACATTAAAACAAAACGTCATAATTATTCA
AGAATAAATNATCATCATCATCAGGCCATTATTAAATAAATNAAAATTTG
sequence update 2001.11.22
Translated Amino Acid sequence 
fsvk*IKKMEKKILLFLLILSISFNNYFVSSTSSSSSSSSECHQKIFSVSEVNPYNINSL
VVIQGIFCLNSTEGIKVSLNVPEEGSNISYGLDCDDSLTIDSIGETITCRLSDNYSGKNA
VNFTTLAIVMVYTPIPVPTPTPSKTPTKQIAVGPVPVLGTSKYIFTNSSC---

---GTTYTNGKPSSKPWLNQLQYDVGFTSVEEPSFSYENYKGAYLPLSSSAATITLTPTI
NINYLPTVYYTTSGGATYSMIFKISNQHDSMSGYSNVFSETSNSITLSSGDGKNSLMVIS
LSDIYSFDQTTAPNHAYLSLLPNPGSSLISNYDSDSLYVSVNIPSIYSTNNQNPYTIQLN
INVYDIDTSSHSKLPKWALGLIVGFSVFIGIVIIFIIAKYIKTKRHNYSRINXHHHQAII
K*xki
Translated Amino Acid sequence (All Frames) 
Frame A:
fsvk*IKKMEKKILLFLLILSISFNNYFVSSTSSSSSSSSECHQKIFSVSEVNPYNINSL
VVIQGIFCLNSTEGIKVSLNVPEEGSNISYGLDCDDSLTIDSIGETITCRLSDNYSGKNA
VNFTTLAIVMVYTPIPVPTPTPSKTPTKQIAVGPVPVLGTSKYIFTNSSC---

---GTTYTNGKPSSKPWLNQLQYDVGFTSVEEPSFSYENYKGAYLPLSSSAATITLTPTI
NINYLPTVYYTTSGGATYSMIFKISNQHDSMSGYSNVFSETSNSITLSSGDGKNSLMVIS
LSDIYSFDQTTAPNHAYLSLLPNPGSSLISNYDSDSLYVSVNIPSIYSTNNQNPYTIQLN
INVYDIDTSSHSKLPKWALGLIVGFSVFIGIVIIFIIAKYIKTKRHNYSRINXHHHQAII
K*xki

Frame B:
lv*nklkkwkkryfyff*fypfhliiilshqhhrhhhhqvnaikrflafqklthtiliv*
llfkvffv*ivqkelkfhsmyqkkdqifhmd*ivmiv*qliqlvkplhvdyqiiivvkmq
*tlqhwql*wfihqfqyqhqhhqklqqnkllldqyqy*elvniysqtvvv---

---vqpiqmvnhlqnhgltncnmmlaslqlrnhhlamkitkvpiyhyhhqqpqll*lqll
istiyqlfiiqlvvvqliq*ylkyrinmtq*vdiqmfslklqiqshyqvemvktl*wllv
ylisivlikqphqimlifhcyqiqvll**aimiqilymfqliyhqsivqtikihiqfn*i
smfmilihhlilnfqngllv*l*vslyssvl*lfsl*lntlkqnviiiqe*xiiiirpll
nkxkf

Frame C:
*ckin*kngkkdtfisfnsihfi**lfcliniiviiiik*mpskdf*rfrs*piqy**fs
cysryflfk*yrrn*sftqctrrrikyfiwirl***fnn*fnw*nhym*iir*l*w*kcs
klynigncnglytnsstntntiknsnktnccwtstsirn**iyihkq*ls---

---ynlykw*tifktma*piai*cwlhfs*gtii*l*klqrclftiiiisshnyfnsny*
yqlftncllyn*wwcnlfndi*niest*lnewifkcfl*nfkfnhiikwrw*klfngy*f
i*yl*f*snnrtksclsfiatksrfffnkql*frfficfs*ytinl*ykqsksiynsiey
qcl*y*yiisf*tskmgswsncrflcihrycnyfhys*ih*nkts*lfknkxssssghy*
inxnl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFJ332 (VFJ332Q) /CSM/VF/VFJ3-B/VFJ332Q.Seq.d/                       2175   0.0
VFJ458 (VFJ458Q) /CSM/VF/VFJ4-C/VFJ458Q.Seq.d/                       1354   0.0
VFL256 (VFL256Q) /CSM/VF/VFL2-C/VFL256Q.Seq.d/                       1197   0.0
VSG630 (VSG630Q) /CSM/VS/VSG6-B/VSG630Q.Seq.d/                       1005   0.0
SFH669 (SFH669Q) /CSM/SF/SFH6-C/SFH669Q.Seq.d/                        125   2e-27
VSD768 (VSD768Q) /CSM/VS/VSD7-C/VSD768Q.Seq.d/                         44   0.005
AFI480 (AFI480Q) /CSM/AF/AFI4-D/AFI480Q.Seq.d/                         44   0.005
AFK862 (AFK862Q) /CSM/AF/AFK8-C/AFK862Q.Seq.d/                         42   0.020
SHB477 (SHB477Q) /CSM/SH/SHB4-D/SHB477Q.Seq.d/                         40   0.080
AHG576 (AHG576Q) /CSM/AH/AHG5-D/AHG576Q.Seq.d/                         40   0.080
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ432694) Dictyostelium discoideum cDNA clone:ddv19p07, 3' ...  1427   0.0    1
(BJ432717) Dictyostelium discoideum cDNA clone:ddv19d16, 3' ...  1354   0.0    1
(BJ434032) Dictyostelium discoideum cDNA clone:ddv23o14, 3' ...  1144   0.0    2
(AU266675) Dictyostelium discoideum vegetative cDNA clone:VS...  1005   0.0    1
(BJ415777) Dictyostelium discoideum cDNA clone:ddv23o14, 5' ...   783   0.0    2
(BJ414524) Dictyostelium discoideum cDNA clone:ddv19p07, 5' ...   783   0.0    2
(BJ414540) Dictyostelium discoideum cDNA clone:ddv19d16, 5' ...   783   0.0    1
(BJ388875) Dictyostelium discoideum cDNA clone:dds16i18, 5' ...    88   9e-54  6
(BJ402378) Dictyostelium discoideum cDNA clone:dds16i18, 3' ...    60   5e-23  5
(AC117072) Dictyostelium discoideum chromosome 2 map 3879572...    36   0.004  12
dna update 2009. 1.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR380949_4(CR380949|pid:none) Candida glabrata strain CBS138 chr...    42   0.060
AY960620_1(AY960620|pid:none) Phragmatopoma californica cement p...    40   0.13
BC153877_1(BC153877|pid:none) Danio rerio zgc:165539, mRNA (cDNA...    39   0.30
(Q54B29) RecName: Full=Uncharacterized membrane protein DDB_G029...    39   0.39
FN357716_11(FN357716|pid:none) Schistosoma mansoni genome sequen...    39   0.39
CP000716_1463(CP000716|pid:none) Thermosipho melanesiensis BI429...    39   0.50
AY170879_1(AY170879|pid:none) Candida albicans isolate AU90 ALS7...    38   0.66
AE014298_2371(AE014298|pid:none) Drosophila melanogaster chromos...    38   0.66
BX842630_10(BX842630|pid:none) Neurospora crassa DNA linkage gro...    38   0.66
AE014187_245(AE014187|pid:none) Plasmodium falciparum 3D7 chromo...    38   0.86
protein update 2009. 6.22
PSORT 

 psg: 1.00  gvh: 0.58  alm: 0.10  top: 0.23  tms: 0.20  mit: 0.43  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 24.0 %: endoplasmic reticulum
	 16.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  8.0 %: nuclear
	  4.0 %: cytoplasmic
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFJ332 is mit
5' end seq. ID VFJ332F
5' end seq. 
>VFJ332F.Seq
TTTAGTGTAAAATAAATTAAAAAAATGGAAAAAAAGATACTTTTATTTCTTTTAATTCTA
TCCATTTCATTTAATAATTATTTTGTCTCATCAACATCATCGTCATCATCATCATCAAGT
GAATGCCATCAAAAGATTTTTAGCGTTTCAGAAGTTAACCCATACAATATTAATAGTTTA
GTTGTTATTCAAGGTATTTTTTGTTTAAATAGTACAGAAGGAATTAAAGTTTCACTCAAT
GTACCAGAAGAAGGATCAAATATTTCATATGGATTAGATTGTGATGATAGTTTAACAATT
GATTCAATTGGTGAAACCATTACATGTAGATTATCAGATAATTATAGTGGTAAAAATGCA
GTAAACTTTACAACATTGGCAATTGTAATGGTTTATACACCAATTCCAGTACCAACACCA
ACACCATCAAAAACTCCAACAAAACAAATTGCTGTTGGACCAGTACCAGTATTAGGAACT
AGTAAATATATATTCACAAACAGTAGTTGTCCNNNNNNNNNN
Length of 5' end seq. 522
3' end seq. ID VFJ332Z
3' end seq. 
>VFJ332Z.Seq
NNNNNNNNNNGGTACAACCTATACAAATGGTAAACCATCTTCAAAACCATGGCTTAACCA
ATTGCAATATGATGTTGGCTTCACTTCAGTTGAGGAACCATCATTTAGCTATGAAAATTA
CAAAGGTGCCTATTTACCATTATCATCATCAGCAGCCACAATTACTTTAACTCCAACTAT
TAATATCAACTATTTACCAACTGTTTATTATACAACTAGTGGTGGTGCAACTTATTCAAT
GATATTTAAAATATCGAATCAACATGACTCAATGAGTGGATATTCAAATGTTTTCTCTGA
AACTTCAAATTCAATCACATTATCAAGTGGAGATGGTAAAAACTCTTTAATGGTTATTAG
TTTATCTGATATCTATAGTTTTGATCAAACAACCGCACCAAATCATGCTTATCTTTCATT
GCTACCAAATCCAGGTTCTTCTTTAATAAGCAATTATGATTCAGATTCTTTATATGTTTC
AGTTAATATACCATCAATCTATAGTACAAACAATCAAAATCCATATACAATTCAATTGAA
TATCAATGTTTATGATATTGATACATCATCTCATTCTAAACTTCCAAAATGGGCTCTTGG
TCTAATTGTAGGTTTCTCTGTATTCATCGGTATTGTAATTATTTTCATTATAGCTAAATA
CATTAAAACAAAACGTCATAATTATTCAAGAATAAATNATCATCATCATCAGGCCATTAT
TAAATAAATNAAAATTTG
Length of 3' end seq. 738
Connected seq. ID VFJ332P
Connected seq. 
>VFJ332P.Seq
TTTAGTGTAAAATAAATTAAAAAAATGGAAAAAAAGATACTTTTATTTCTTTTAATTCTA
TCCATTTCATTTAATAATTATTTTGTCTCATCAACATCATCGTCATCATCATCATCAAGT
GAATGCCATCAAAAGATTTTTAGCGTTTCAGAAGTTAACCCATACAATATTAATAGTTTA
GTTGTTATTCAAGGTATTTTTTGTTTAAATAGTACAGAAGGAATTAAAGTTTCACTCAAT
GTACCAGAAGAAGGATCAAATATTTCATATGGATTAGATTGTGATGATAGTTTAACAATT
GATTCAATTGGTGAAACCATTACATGTAGATTATCAGATAATTATAGTGGTAAAAATGCA
GTAAACTTTACAACATTGGCAATTGTAATGGTTTATACACCAATTCCAGTACCAACACCA
ACACCATCAAAAACTCCAACAAAACAAATTGCTGTTGGACCAGTACCAGTATTAGGAACT
AGTAAATATATATTCACAAACAGTAGTTGTCC----------GGTACAACCTATACAAAT
GGTAAACCATCTTCAAAACCATGGCTTAACCAATTGCAATATGATGTTGGCTTCACTTCA
GTTGAGGAACCATCATTTAGCTATGAAAATTACAAAGGTGCCTATTTACCATTATCATCA
TCAGCAGCCACAATTACTTTAACTCCAACTATTAATATCAACTATTTACCAACTGTTTAT
TATACAACTAGTGGTGGTGCAACTTATTCAATGATATTTAAAATATCGAATCAACATGAC
TCAATGAGTGGATATTCAAATGTTTTCTCTGAAACTTCAAATTCAATCACATTATCAAGT
GGAGATGGTAAAAACTCTTTAATGGTTATTAGTTTATCTGATATCTATAGTTTTGATCAA
ACAACCGCACCAAATCATGCTTATCTTTCATTGCTACCAAATCCAGGTTCTTCTTTAATA
AGCAATTATGATTCAGATTCTTTATATGTTTCAGTTAATATACCATCAATCTATAGTACA
AACAATCAAAATCCATATACAATTCAATTGAATATCAATGTTTATGATATTGATACATCA
TCTCATTCTAAACTTCCAAAATGGGCTCTTGGTCTAATTGTAGGTTTCTCTGTATTCATC
GGTATTGTAATTATTTTCATTATAGCTAAATACATTAAAACAAAACGTCATAATTATTCA
AGAATAAATNATCATCATCATCAGGCCATTATTAAATAAATNAAAATTTG
Length of connected seq. 1240
Full length Seq ID -
Full length Seq. -
Length of full length seq. -