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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFL723
Library VF
(Link to library)
Clone ID VFL723
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12230-1
Original site URL
Representative seq. ID VFL723P
(Link to Original site)
Representative DNA sequence 
>VFL723 (VFL723Q) /CSM/VF/VFL7-A/VFL723Q.Seq.d/
CACTGTTGGCCTAAATTTTATTTTTTTAAAATATCAATATTAAAATTTAAAATACTATTC
AACAAACGATAAATATATATATTTGTTTTCATTATTTATTTATTTATTAATTTAAATAGT
ATAAACAAAATGGGAAAGGATTATACATTTATTAGAAATTGGATTAGTTTCCATTTTATG
GGAAATATTAATAATTTTTCATATTGCGTTGTAAATGCAGCAAGTGGTAACTTATCAGCT
TATTTTCACAACTCTAAAAATATTGGAATTATTCTTTGGGCAAATATTGCATTTGGTTTA
GTTTCAAGATTGGTTAATACATTTTTAATTGAAAATGTTAATTCGAAATTAAAGATAGTA
GTAAATTGTTTATTTATGTCAATTGGTTTAATTGGTGTAGCATTATCAGTATATGTTAAT
TTTGGATTTTGTATAGCAGCCATAGCATTTGTTGGTATTGCAAGTTCATTTGGTGAGAGT
GTAATTCTCTCATACATGAAGAAATTTCCTGCANAGCTAGNTAATGGNNGGNCAAGNGGA
CAGGTATTGCAGGNGNATGNGGNTNATTGNNNNATATXXXXXXXXXXCAATAGCAGCAAT
AACAGCAAATATACAGAGAAACAATCATTGGTTAAAAAGGACCAACTTATTGAAGATATA
GATGATGGTGGTGGTGCTGAAGTTGCACCACCCAGGTGAAACTAAAAAACAAAGATATAT
TCGTTGTGCACGTTTAGTTTGGTTCAATGCTGTCAATTTAGCATTGGTTTATTTCTTTGA
ATATGTTGCAAGTGTAGGTGGTGCTGATCTTGCCTTGAAAAAGGATCCAAACAATGATAA
TTTCTTCATTGCAAATGCATTTGCAATCTTTAGTTTTTGTTATCAATTGGGTGTATTAAT
CTCACGTTCTTCACTTCAATTCGTTAAAATCAAACATATTGGTGTTATCACCATCCTTCA
AGGTATTAATATGGTCTTTTGGATCATACAAGCAAAATATAAAATGGTCACCTCTGTTTG
GGTACTCTTTATTCTCATGGTTTATTGTGGTCTTTTAGGTGGTGCTTCTTATGTTAATGT
ATTTTACTTAATTCTTCATCAAAAAAATATTCCAAATGAAGATAGAGAACTTTGCATTAA
TTATGCTGCTTTATTAGTTACTGTTGGTATTACACTCGCAGCTTGTTTCATTCTCGTTAT
GGACCATACATTTTTAGCCNCTGAGGTTCCAAAAACNATTCTTCATCCTCAACATAAATA
AAATTTTTTA
sequence update 2001.11.22
Translated Amino Acid sequence 
hcwpkfyffkisilkfkilfnkr*IYIFVFIIYLFINLNSINKMGKDYTFIRNWISFHFM
GNINNFSYCVVNAASGNLSAYFHNSKNIGIILWANIAFGLVSRLVNTFLIENVNSKLKIV
VNCLFMSIGLIGVALSVYVNFGFCIAAIAFVGIASSFGESVILSYMKKFPAXLXNGXXXG
QVLQXXXXXXXI---

---NSSNNSKYTEKQSLVKKDQLIEDIDDGGGAEVAPPR*n*ktkiyslctfslvqccqf
siglfl*icckcrwc*sclekgskq**flhckcicnl*fllsigcinltfftsir*nqty
wcyhhpsry*yglldhtski*nghlclgtlyshgllwsfrwcflc*cillnssskkysk*
r*rtlh*lccfisycwyytrslfhsrygpyifsx*gsknxsssst*ikff
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwpkfyffkisilkfkilfnkr*IYIFVFIIYLFINLNSINKMGKDYTFIRNWISFHFM
GNINNFSYCVVNAASGNLSAYFHNSKNIGIILWANIAFGLVSRLVNTFLIENVNSKLKIV
VNCLFMSIGLIGVALSVYVNFGFCIAAIAFVGIASSFGESVILSYMKKFPAXLXNGXXXG
QVLQXXXXXXXI---

---q*qq*qqiyretiig*kgpty*ryr*wwwc*scttqvklknkdifvvhv*fgsmlsi
*hwfislnmlqv*vvlilp*kriqtmiisslqmhlqslvfvinwvy*shvlhfnslksni
lvlspsfkvliwsfgsykqnikwsplfgyslfswfivvf*vvllmlmyft*ffikkifqm
kienfalimlly*lllvlhsqlvsfslwtihf*plrfqkxffilninkif

Frame B:
tvglnfiflkyqy*nlkyystndkyiylfslfiylli*iv*tkweriihlleiglvsilw
eiliifhial*mqqvvtyqlifttlkilelffgqilhlv*fqdwlihf*lkmlirn*r**
*ivylcqlv*lv*hyqymlildfv*qp*hllvlqvhlvrv*fsht*rnflxs*xmxgqxd
rycrxmxxixxy---

---NSSNNSKYTEKQSLVKKDQLIEDIDDGGGAEVAPPR*n*ktkiyslctfslvqccqf
siglfl*icckcrwc*sclekgskq**flhckcicnl*fllsigcinltfftsir*nqty
wcyhhpsry*yglldhtski*nghlclgtlyshgllwsfrwcflc*cillnssskkysk*
r*rtlh*lccfisycwyytrslfhsrygpyifsx*gsknxsssst*ikff

Frame C:
lla*ilff*niniki*ntiqqtiniyicfhylfiy*fk*ykqngkglyiy*kld*fpfyg
ky**ffilrckcskw*lislfsql*kywnyslgkyciwfsfkig*yifn*kc*feikdss
klfiyvnwfnwcsiisic*fwilysshsicwyckfiw*ecnsliheeiscxax*wxxkxt
giagxxgxlxx---

---iaaitaniqrnnhwlkrtnllki*mmvvvlklhhpgetkkqryircarlvwfnavnl
alvyffeyvasvggadlalkkdpnndnffianafaifsfcyqlgvlisrsslqfvkikhi
gvitilqginmvfwiiqakykmvtsvwvlfilmvycgllggasyvnvfylilhqknipne
drelcinyaallvtvgitlaacfilvmdhtflaxevpktilhpqhk*nfl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL723 (VFL723Q) /CSM/VF/VFL7-A/VFL723Q.Seq.d/                       2089   0.0
SFI873 (SFI873Q) /CSM/SF/SFI8-D/SFI873Q.Seq.d/                       1285   0.0
SFC282 (SFC282Q) /CSM/SF/SFC2-D/SFC282Q.Seq.d/                       1285   0.0
AFF192 (AFF192Q) /CSM/AF/AFF1-D/AFF192Q.Seq.d/                       1285   0.0
SLB574 (SLB574Q) /CSM/SL/SLB5-D/SLB574Q.Seq.d/                       1271   0.0
SFF259 (SFF259Q) /CSM/SF/SFF2-C/SFF259Q.Seq.d/                       1269   0.0
CHC249 (CHC249Q) /CSM/CH/CHC2-C/CHC249Q.Seq.d/                       1269   0.0
AFM629 (AFM629Q) /CSM/AF/AFM6-B/AFM629Q.Seq.d/                       1269   0.0
VSG493 (VSG493Q) /CSM/VS/VSG4-D/VSG493Q.Seq.d/                       1217   0.0
CFD301 (CFD301Q) /CSM/CF/CFD3-A/CFD301Q.Seq.d/                       1187   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ434545) Dictyostelium discoideum cDNA clone:ddv24n05, 3' ...  1017   0.0    2
(BJ402935) Dictyostelium discoideum cDNA clone:dds18b20, 3' ...   811   0.0    3
(BJ400914) Dictyostelium discoideum cDNA clone:dds15k10, 3' ...   811   0.0    3
(BJ399493) Dictyostelium discoideum cDNA clone:dds6a07, 3' e...   811   0.0    5
(BJ399400) Dictyostelium discoideum cDNA clone:dds5c22, 3' e...   811   0.0    3
(BJ398697) Dictyostelium discoideum cDNA clone:dds1m14, 3' e...   811   0.0    4
(BJ398226) Dictyostelium discoideum cDNA clone:dds11e16, 3' ...   811   0.0    4
(BJ377935) Dictyostelium discoideum cDNA clone:ddc26a14, 3' ...   811   0.0    4
(BJ374271) Dictyostelium discoideum cDNA clone:ddc7b01, 3' e...   811   0.0    4
(BJ347183) Dictyostelium discoideum cDNA clone:dda26i08, 3' ...   811   0.0    4
dna update 2009. 3.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM502224_134(AM502224|pid:none) Leishmania infantum chromosome 6.      98   7e-19
(Q6BZW3) RecName: Full=Protein BTN1; Flags: Precursor;  &CR38213...    93   2e-17
(Q2GN49) RecName: Full=Protein BTN1;                                   91   9e-17
AC105378_11(AC105378|pid:none) Trypanosoma brucei chromosome 7 c...    87   1e-15
(Q29611) RecName: Full=Battenin; AltName: Full=Protein CLN3;  &A...    84   1e-14
(Q758C3) RecName: Full=Protein BTN1; Flags: Precursor;  &AE01681...    83   2e-14
(Q4HX89) RecName: Full=Protein BTN1;                                   81   7e-14
FM992688_482(FM992688|pid:none) Candida dubliniensis CD36 chromo...    80   1e-13
(Q59LX9) RecName: Full=Protein BTN1; Flags: Precursor;                 80   2e-13
CU928180_447(CU928180|pid:none) Kluyveromyces thermotolerans str...    79   3e-13
protein update 2009. 6.23
PSORT 

 psg: 0.98  gvh: 0.54  alm: 0.22  top: 0.60  tms: 0.27  mit: 0.22  mip: 0.14
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 36.0 %: plasma membrane
	 24.0 %: endoplasmic reticulum
	 12.0 %: mitochondrial
	  8.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for VFL723 is pla
5' end seq. ID VFL723F
5' end seq. 
>VFL723F.Seq
CACTGTTGGCCTAAATTTTATTTTTTTAAAATATCAATATTAAAATTTAAAATACTATTC
AACAAACGATAAATATATATATTTGTTTTCATTATTTATTTATTTATTAATTTAAATAGT
ATAAACAAAATGGGAAAGGATTATACATTTATTAGAAATTGGATTAGTTTCCATTTTATG
GGAAATATTAATAATTTTTCATATTGCGTTGTAAATGCAGCAAGTGGTAACTTATCAGCT
TATTTTCACAACTCTAAAAATATTGGAATTATTCTTTGGGCAAATATTGCATTTGGTTTA
GTTTCAAGATTGGTTAATACATTTTTAATTGAAAATGTTAATTCGAAATTAAAGATAGTA
GTAAATTGTTTATTTATGTCAATTGGTTTAATTGGTGTAGCATTATCAGTATATGTTAAT
TTTGGATTTTGTATAGCAGCCATAGCATTTGTTGGTATTGCAAGTTCATTTGGTGAGAGT
GTAATTCTCTCATACATGAAGAAATTTCCTGCANAGCTAGNTAATGGNNGGNCAAGNGGA
CAGGTATTGCAGGNGNATGNGGNTNATTGNNNNATATNNNNNNNNNN
Length of 5' end seq. 587
3' end seq. ID VFL723Z
3' end seq. 
>VFL723Z.Seq
NNNNNNNNNNCAATAGCAGCAATAACAGCAAATATACAGAGAAACAATCATTGGTTAAAA
AGGACCAACTTATTGAAGATATAGATGATGGTGGTGGTGCTGAAGTTGCACCACCCAGGT
GAAACTAAAAAACAAAGATATATTCGTTGTGCACGTTTAGTTTGGTTCAATGCTGTCAAT
TTAGCATTGGTTTATTTCTTTGAATATGTTGCAAGTGTAGGTGGTGCTGATCTTGCCTTG
AAAAAGGATCCAAACAATGATAATTTCTTCATTGCAAATGCATTTGCAATCTTTAGTTTT
TGTTATCAATTGGGTGTATTAATCTCACGTTCTTCACTTCAATTCGTTAAAATCAAACAT
ATTGGTGTTATCACCATCCTTCAAGGTATTAATATGGTCTTTTGGATCATACAAGCAAAA
TATAAAATGGTCACCTCTGTTTGGGTACTCTTTATTCTCATGGTTTATTGTGGTCTTTTA
GGTGGTGCTTCTTATGTTAATGTATTTTACTTAATTCTTCATCAAAAAAATATTCCAAAT
GAAGATAGAGAACTTTGCATTAATTATGCTGCTTTATTAGTTACTGTTGGTATTACACTC
GCAGCTTGTTTCATTCTCGTTATGGACCATACATTTTTAGCCNCTGAGGTTCCAAAAACN
ATTCTTCATCCTCAACATAAATAAAATTTTTTA
Length of 3' end seq. 693
Connected seq. ID VFL723P
Connected seq. 
>VFL723P.Seq
CACTGTTGGCCTAAATTTTATTTTTTTAAAATATCAATATTAAAATTTAAAATACTATTC
AACAAACGATAAATATATATATTTGTTTTCATTATTTATTTATTTATTAATTTAAATAGT
ATAAACAAAATGGGAAAGGATTATACATTTATTAGAAATTGGATTAGTTTCCATTTTATG
GGAAATATTAATAATTTTTCATATTGCGTTGTAAATGCAGCAAGTGGTAACTTATCAGCT
TATTTTCACAACTCTAAAAATATTGGAATTATTCTTTGGGCAAATATTGCATTTGGTTTA
GTTTCAAGATTGGTTAATACATTTTTAATTGAAAATGTTAATTCGAAATTAAAGATAGTA
GTAAATTGTTTATTTATGTCAATTGGTTTAATTGGTGTAGCATTATCAGTATATGTTAAT
TTTGGATTTTGTATAGCAGCCATAGCATTTGTTGGTATTGCAAGTTCATTTGGTGAGAGT
GTAATTCTCTCATACATGAAGAAATTTCCTGCANAGCTAGNTAATGGNNGGNCAAGNGGA
CAGGTATTGCAGGNGNATGNGGNTNATTGNNNNATAT----------CAATAGCAGCAAT
AACAGCAAATATACAGAGAAACAATCATTGGTTAAAAAGGACCAACTTATTGAAGATATA
GATGATGGTGGTGGTGCTGAAGTTGCACCACCCAGGTGAAACTAAAAAACAAAGATATAT
TCGTTGTGCACGTTTAGTTTGGTTCAATGCTGTCAATTTAGCATTGGTTTATTTCTTTGA
ATATGTTGCAAGTGTAGGTGGTGCTGATCTTGCCTTGAAAAAGGATCCAAACAATGATAA
TTTCTTCATTGCAAATGCATTTGCAATCTTTAGTTTTTGTTATCAATTGGGTGTATTAAT
CTCACGTTCTTCACTTCAATTCGTTAAAATCAAACATATTGGTGTTATCACCATCCTTCA
AGGTATTAATATGGTCTTTTGGATCATACAAGCAAAATATAAAATGGTCACCTCTGTTTG
GGTACTCTTTATTCTCATGGTTTATTGTGGTCTTTTAGGTGGTGCTTCTTATGTTAATGT
ATTTTACTTAATTCTTCATCAAAAAAATATTCCAAATGAAGATAGAGAACTTTGCATTAA
TTATGCTGCTTTATTAGTTACTGTTGGTATTACACTCGCAGCTTGTTTCATTCTCGTTAT
GGACCATACATTTTTAGCCNCTGAGGTTCCAAAAACNATTCTTCATCCTCAACATAAATA
AAATTTTTTA
Length of connected seq. 1260
Full length Seq ID -
Full length Seq. -
Length of full length seq. -