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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFO109
Library VF
(Link to library)
Clone ID VFO109
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16590-1
Original site URL
Representative seq. ID VFO109P
(Link to Original site)
Representative DNA sequence 
>VFO109 (VFO109Q) /CSM/VF/VFO1-A/VFO109Q.Seq.d/
ATGTTAAAAAATATTATTAATAAAAAGAATTTATTTATTAATAATCAACAAAACATCTTT
CAAATAATTAAAAGAAATAAAATGACAGATAGTNCAGTTGATAATAAAGGATATATTGTA
GGTATNTACGAGAATGAAGAGTTTACACCATTAGGTCAACAGTTAAATGAAAAGACAAAT
GGTCATTTATTGAAGAGTNTTAAATTATCAGATACCAAAGGTAAAGTTGGAGATAATTTA
GTTTTATACAATGTCACACCAGAGGTTTCAAGAGTTGCAATCGTTGGCTTGGGTAAGAAA
GAAAATAATAATAGCACCACCTATGAAAAGAATGAAAACACTCGTAAAGCAATCGGTAGT
GGTGTTAAAGCATTGAAATCAAAGAATGCCACCCATTTAACCATCGATTCAAACATTGGT
GATGCCAAACAAACTGCAGAGGGTGCATTCCTTTCAAACTTCAAATTTGATTTCAAAACA
GGTACTTXXXXXXXXXXAGGGTCGTTTAATCTTGGGTGATGCTTTACATTATGCTTGTTC
ATTCAAACCAACTCATATCATTGATATCGCTACCTTGACTGGTGCCATCGATGTTGCCTT
GGGTCAACATTATGCTGGTTGTTTTACAACCACCGACTCACTTTGGGATCAATTAAATGA
ATGTGGTAACATTAGTGGTGAAAGATTATGGAGAATGCCATTGATTCCAGAATATCGTAA
ACAAATGGAAACCTCAAAAGTTGCCGATTTAATCAATTCTGCTGGTCGTTCAGGTGGTGC
TTGTTGTGCTGCTGGTTTCCTTAAAGAATTCATTACAGCCGATCAATCTTGGTCTCACCT
TGATATTGCTGGTGTTATGTCATCATCTGAAGAGGGTCCATACATTAGAAAAGGTATGAC
TGGTAAACCAACTCGTACTTTAATAGAATTTGCTAAAAAGAATCAACAATAATAATTATT
AAAATAAAAAAAAAAAATAAAAAAATAAAAAGTAAAAGTTC
sequence update 2001.11.22
Translated Amino Acid sequence 
MLKNIINKKNLFINNQQNIFQIIKRNKMTDSXVDNKGYIVGXYENEEFTPLGQQLNEKTN
GHLLKSXKLSDTKGKVGDNLVLYNVTPEVSRVAIVGLGKKENNNSTTYEKNENTRKAIGS
GVKALKSKNATHLTIDSNIGDAKQTAEGAFLSNFKFDFKTGT---

---GRLILGDALHYACSFKPTHIIDIATLTGAIDVALGQHYAGCFTTTDSLWDQLNECGN
ISGERLWRMPLIPEYRKQMETSKVADLINSAGRSGGACCAAGFLKEFITADQSWSHLDIA
GVMSSSEEGPYIRKGMTGKPTRTLIEFAKKNQQ**llk*kkkikk*kvkv
Translated Amino Acid sequence (All Frames) 
Frame A:
MLKNIINKKNLFINNQQNIFQIIKRNKMTDSXVDNKGYIVGXYENEEFTPLGQQLNEKTN
GHLLKSXKLSDTKGKVGDNLVLYNVTPEVSRVAIVGLGKKENNNSTTYEKNENTRKAIGS
GVKALKSKNATHLTIDSNIGDAKQTAEGAFLSNFKFDFKTGT---

---rvv*swvmlyimlvhsnqlislislp*lvpsmlpwvnimlvvlqppthfgin*mnvv
tlvvkdygech*fqnivnkwkpqklpi*sillvvqvvlvvllvslknslqpinlgltlil
lvlchhlkrvhtlekv*lvnqlvl**nllkrinnnny*nkkkk*knkk*kf

Frame B:
c*killikriylliinktsfk*lkeik*qivqliikdil*vxtrmkslhh*vns*mkrqm
viy*rvlnyqipkvkleii*fytmshqrfqelqslawvrkkiiiappmkrmktlvkqsvv
vlkh*nqrmppi*psiqtlvmpnklqrvhsfqtsnliskqvl---

---gsfnlg*cftlclfiqtnsyh*yryldwchrcclgstlcwlfynhrltlgsik*mw*
h*w*kimenaidsris*tngnlkscrfnqfcwsfrwcllccwfp*rihysrsilvsp*yc
wcyvii*rgsih*krydw*tnsyfnric*kestiiiikikkknkkiksks

Frame C:
vkkyy**kefiy**stkhlsnn*kk*ndr*xs***riycryxre*rvytirstvk*kdkw
sfieex*iiryqr*swr*fsfiqchtrgfkscnrwlg*erk***hhl*ke*khs*snr*w
c*sieikechpfnhrfkhw*cqtncrgcipfklqi*fqnry---

---GRLILGDALHYACSFKPTHIIDIATLTGAIDVALGQHYAGCFTTTDSLWDQLNECGN
ISGERLWRMPLIPEYRKQMETSKVADLINSAGRSGGACCAAGFLKEFITADQSWSHLDIA
GVMSSSEEGPYIRKGMTGKPTRTLIEFAKKNQQ**llk*kkkikk*kvkv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO109 (VFO109Q) /CSM/VF/VFO1-A/VFO109Q.Seq.d/                       1826   0.0
VFO794 (VFO794Q) /CSM/VF/VFO7-D/VFO794Q.Seq.d/                        948   0.0
VFN379 (VFN379Q) /CSM/VF/VFN3-D/VFN379Q.Seq.d/                        948   0.0
VFM231 (VFM231Q) /CSM/VF/VFM2-B/VFM231Q.Seq.d/                        948   0.0
VFF745 (VFF745Q) /CSM/VF/VFF7-B/VFF745Q.Seq.d/                        948   0.0
CHA589 (CHA589Q) /CSM/CH/CHA5-D/CHA589Q.Seq.d/                        948   0.0
AFO810 (AFO810Q) /CSM/AF/AFO8-A/AFO810Q.Seq.d/                        948   0.0
AFO318 (AFO318Q) /CSM/AF/AFO3-A/AFO318Q.Seq.d/                        948   0.0
AFL713 (AFL713Q) /CSM/AF/AFL7-A/AFL713Q.Seq.d/                        948   0.0
AFJ681 (AFJ681Q) /CSM/AF/AFJ6-D/AFJ681Q.Seq.d/                        948   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AY581145) Dictyostelium discoideum leucine aminopeptidase (...   894   0.0    2
(BJ435924) Dictyostelium discoideum cDNA clone:ddv29a03, 3' ...   918   0.0    1
(AU034864) Dictyostelium discoideum slug cDNA, clone SLE612.      900   0.0    1
(C93130) Dictyostelium discoideum slug cDNA, clone SSM588.        900   0.0    1
(BJ434283) Dictyostelium discoideum cDNA clone:ddv16m08, 3' ...   900   0.0    1
(BJ428545) Dictyostelium discoideum cDNA clone:ddv12j11, 3' ...   900   0.0    1
(BJ428433) Dictyostelium discoideum cDNA clone:ddv11m20, 3' ...   900   0.0    1
(BJ343013) Dictyostelium discoideum cDNA clone:dda15g23, 3' ...   894   0.0    1
(BJ341105) Dictyostelium discoideum cDNA clone:dda5a08, 3' e...   894   0.0    1
(BJ344492) Dictyostelium discoideum cDNA clone:dda19c17, 3' ...   797   0.0    2
dna update 2009. 5.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q5V9F0) RecName: Full=Cytosol aminopeptidase;          EC=3.4.1...   278   3e-73
BC068707_1(BC068707|pid:none) Xenopus laevis hypothetical protei...   154   3e-36
BX901913_3(BX901913|pid:none) Zebrafish DNA sequence from clone ...   153   1e-35
AJ720335_1(AJ720335|pid:none) Gallus gallus mRNA for hypothetica...   144   4e-33
AF061738_1(AF061738|pid:none) Homo sapiens leucine aminopeptidas...   141   3e-32
AK022055_1(AK022055|pid:none) Homo sapiens cDNA FLJ11993 fis, cl...   141   3e-32
AB170537_1(AB170537|pid:none) Macaca fascicularis brain cDNA clo...   141   4e-32
FJ347751_1(FJ347751|pid:none) Crassostrea gigas leucine aminopep...   139   1e-31
FJ347745_1(FJ347745|pid:none) Crassostrea gigas leucine aminopep...   139   1e-31
FJ347727_1(FJ347727|pid:none) Crassostrea gigas leucine aminopep...   139   1e-31
protein update 2009. 6.25
PSORT 

 psg: 0.71  gvh: 0.24  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.42  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VFO109 is nuc
5' end seq. ID VFO109F
5' end seq. 
>VFO109F.Seq
ATGTTAAAAAATATTATTAATAAAAAGAATTTATTTATTAATAATCAACAAAACATCTTT
CAAATAATTAAAAGAAATAAAATGACAGATAGTNCAGTTGATAATAAAGGATATATTGTA
GGTATNTACGAGAATGAAGAGTTTACACCATTAGGTCAACAGTTAAATGAAAAGACAAAT
GGTCATTTATTGAAGAGTNTTAAATTATCAGATACCAAAGGTAAAGTTGGAGATAATTTA
GTTTTATACAATGTCACACCAGAGGTTTCAAGAGTTGCAATCGTTGGCTTGGGTAAGAAA
GAAAATAATAATAGCACCACCTATGAAAAGAATGAAAACACTCGTAAAGCAATCGGTAGT
GGTGTTAAAGCATTGAAATCAAAGAATGCCACCCATTTAACCATCGATTCAAACATTGGT
GATGCCAAACAAACTGCAGAGGGTGCATTCCTTTCAAACTTCAAATTTGATTTCAAAACA
GGTACTTNNNNNNNNNN
Length of 5' end seq. 497
3' end seq. ID VFO109Z
3' end seq. 
>VFO109Z.Seq
NNNNNNNNNNAGGGTCGTTTAATCTTGGGTGATGCTTTACATTATGCTTGTTCATTCAAA
CCAACTCATATCATTGATATCGCTACCTTGACTGGTGCCATCGATGTTGCCTTGGGTCAA
CATTATGCTGGTTGTTTTACAACCACCGACTCACTTTGGGATCAATTAAATGAATGTGGT
AACATTAGTGGTGAAAGATTATGGAGAATGCCATTGATTCCAGAATATCGTAAACAAATG
GAAACCTCAAAAGTTGCCGATTTAATCAATTCTGCTGGTCGTTCAGGTGGTGCTTGTTGT
GCTGCTGGTTTCCTTAAAGAATTCATTACAGCCGATCAATCTTGGTCTCACCTTGATATT
GCTGGTGTTATGTCATCATCTGAAGAGGGTCCATACATTAGAAAAGGTATGACTGGTAAA
CCAACTCGTACTTTAATAGAATTTGCTAAAAAGAATCAACAATAATAATTATTAAAATAA
AAAAAAAAAATAAAAAAATAAAAAGTAAAAGTTC
Length of 3' end seq. 514
Connected seq. ID VFO109P
Connected seq. 
>VFO109P.Seq
ATGTTAAAAAATATTATTAATAAAAAGAATTTATTTATTAATAATCAACAAAACATCTTT
CAAATAATTAAAAGAAATAAAATGACAGATAGTNCAGTTGATAATAAAGGATATATTGTA
GGTATNTACGAGAATGAAGAGTTTACACCATTAGGTCAACAGTTAAATGAAAAGACAAAT
GGTCATTTATTGAAGAGTNTTAAATTATCAGATACCAAAGGTAAAGTTGGAGATAATTTA
GTTTTATACAATGTCACACCAGAGGTTTCAAGAGTTGCAATCGTTGGCTTGGGTAAGAAA
GAAAATAATAATAGCACCACCTATGAAAAGAATGAAAACACTCGTAAAGCAATCGGTAGT
GGTGTTAAAGCATTGAAATCAAAGAATGCCACCCATTTAACCATCGATTCAAACATTGGT
GATGCCAAACAAACTGCAGAGGGTGCATTCCTTTCAAACTTCAAATTTGATTTCAAAACA
GGTACTT----------AGGGTCGTTTAATCTTGGGTGATGCTTTACATTATGCTTGTTC
ATTCAAACCAACTCATATCATTGATATCGCTACCTTGACTGGTGCCATCGATGTTGCCTT
GGGTCAACATTATGCTGGTTGTTTTACAACCACCGACTCACTTTGGGATCAATTAAATGA
ATGTGGTAACATTAGTGGTGAAAGATTATGGAGAATGCCATTGATTCCAGAATATCGTAA
ACAAATGGAAACCTCAAAAGTTGCCGATTTAATCAATTCTGCTGGTCGTTCAGGTGGTGC
TTGTTGTGCTGCTGGTTTCCTTAAAGAATTCATTACAGCCGATCAATCTTGGTCTCACCT
TGATATTGCTGGTGTTATGTCATCATCTGAAGAGGGTCCATACATTAGAAAAGGTATGAC
TGGTAAACCAACTCGTACTTTAATAGAATTTGCTAAAAAGAATCAACAATAATAATTATT
AAAATAAAAAAAAAAAATAAAAAAATAAAAAGTAAAAGTTC
Length of connected seq. 991
Full length Seq ID -
Full length Seq. -
Length of full length seq. -