>VHA224 (VHA224Q) /CSM/VH/VHA2-A/VHA224Q.Seq.d/
AATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGAT
AAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAA
CTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCA
GTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGG
GAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGAT
TTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTA
GGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACTCT
GTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAATTC
CATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGATTA
TTTATTGCTCCXXXXXXXXXXTGATAGTATTACCACTGATCATATTTCACCAGCCGGTAA
CATCAATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGA
TTTTAACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGC
CAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACC
AACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCA
ACTCATTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAA
AGGTCCATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAG
ATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTC
TACTTTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCT
CATTAAAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTAT
CCTTCGTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTA
TGTTTTAAGAAAACTTTTACATTAATTTCTTTTTTTAATATAAAATAAAAAAAAATAATA
TTATTTAAAA

NFNLYKLPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLESA
VRNCDNFEVHEKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKRL
GGDPAKINPLVPVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDGL
FIA---

---DSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRI
VNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYL
QGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTG
QTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*yylk

Frame A:
NFNLYKLPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLESA
VRNCDNFEVHEKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKRL
GGDPAKINPLVPVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDGL
FIA---

---**yyh*syftsr*hq*kiisrslfrvqrcktrrf*hlwis*r***nyg*rylcqysy
c**igssrwstnnlctnw*vnvyfrccrkisirrssthcfgw**lwlw*f*rlgcqrsip
srn*vchchli*tys*iqsrwygyypiaiptrskclyfgshw*rtiqyrvtn**iph*nw
snrkgyh*lw*if*nypsfrysnrs*ilgk*wylilcfkktftlisffnik*kkiilfk

Frame B:
iliyinylit*r*qqiihlik*rmy*nhkikliifiifqnfktqelknyhtpleffwnqq
fvivitlkytkkmlkis*igktlqiklkflsnqqeyyykillvyqqllilqq*emp*kd*
vviqqklthlfqli*llitlfklmyqellml*skikrlnsieimkdfhf*ngvlkhlmdy
lll---

---DSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRI
VNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYL
QGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTG
QTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*yylk

Frame C:
f*fi*itlllkddnk*si**skgcikitrsnl*fl*sfktsrpkn*kitilh*nsfgiss
s*l**l*strkrc*kylklgkhck*s*nsfqtsksiitrfywctssc*scsnerchekir
w*sskn*ptcss*fsy*slcss*ciknc*cfrakskd*ip*ks*kififkmgcssi*wii
ycs---

---ivlpliifhqpvtsienhqplvi*spkv*nqkiltpmdlvevmmklwlevplpilvl
linwlqplvhkqpmyqlvs*clfqmlqkninqkvinslfwlvvimalvvleiglpkvhtf
kelsvslpshlnvfidpislvwvlshcnsnqvkmpllwvslvknnsissyqlinpslklv
kp*rlplivvnllklsfvsilqsklnigqimvsylmf*enfyinfff*ykikknnii*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA224 (VHA224Q) /CSM/VH/VHA2-A/VHA224Q.Seq.d/                       2377   0.0
VHQ461 (VHQ461Q) /CSM/VH/VHQ4-C/VHQ461Q.Seq.d/                       1324   0.0
VHQ432 (VHQ432Q) /CSM/VH/VHQ4-B/VHQ432Q.Seq.d/                       1324   0.0
VHQ267 (VHQ267Q) /CSM/VH/VHQ2-C/VHQ267Q.Seq.d/                       1324   0.0
VHO844 (VHO844Q) /CSM/VH/VHO8-B/VHO844Q.Seq.d/                       1324   0.0
VHO678 (VHO678Q) /CSM/VH/VHO6-D/VHO678Q.Seq.d/                       1324   0.0
VHK789 (VHK789Q) /CSM/VH/VHK7-D/VHK789Q.Seq.d/                       1324   0.0
VHK111 (VHK111Q) /CSM/VH/VHK1-A/VHK111Q.Seq.d/                       1324   0.0
VHE337 (VHE337Q) /CSM/VH/VHE3-B/VHE337Q.Seq.d/                       1324   0.0
VHA714 (VHA714Q) /CSM/VH/VHA7-A/VHA714Q.Seq.d/                       1324   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF194945|AF194945.1 Nicotiana tabacum cytosolic aconitase (ACO) mRNA, complete cds.    58   1e-31  6
AY250116|AY250116.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   5e-26  5
EJ685842|EJ685842.1 1092955177152 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061001864069 5', genomic survey sequence.    82   2e-22  2
AY250115|AY250115.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   2e-21  5
BE579347|BE579347.1 kq26c09.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:ZK455.1 CE03812 PROBABLE ACONITATE HYDRATASE, CYTOPLASMIC ;, mRNA sequence.    64   2e-20  3
CO910019|CO910019.1 BJ02055G09 BJ02 Capsicum annuum cDNA 5', mRNA sequence.    58   1e-18  4
CK265172|CK265172.1 EST711250 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POABX20 5' end, mRNA sequence.    58   1e-17  4
DB747232|DB747232.1 Apis mellifera head cDNA, RIKEN full-length enriched library, clone:BH10068C02, 5' end partial sequence.    70   3e-17  4
AY062772|AY062772.1 Arabidopsis thaliana Unknown protein (At4g35830; F4B14.100) mRNA, complete cds.    46   5e-17  6
DB747931|DB747931.1 Apis mellifera head cDNA, RIKEN full-length enriched library, clone:BH10070K18, 5' end partial sequence.    70   1e-16  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   443   e-123
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   291   3e-77
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   290   8e-77
BT060820_1(BT060820|pid:none) Zea mays full-length cDNA clone ZM...   288   3e-76
BT060665_1(BT060665|pid:none) Zea mays full-length cDNA clone ZM...   287   5e-76
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   287   5e-76
(P49608) RecName: Full=Aconitate hydratase, cytoplasmic;        ...   284   4e-75
DQ294230_1(DQ294230|pid:none) Glossina morsitans morsitans TC165...   283   7e-75
(Q9SIB9) RecName: Full=Aconitate hydratase 2, mitochondrial;    ...   283   9e-75
AY050485_1(AY050485|pid:none) Prunus avium putative aconitase mR...   282   2e-74

 psg: 0.75  gvh: 0.20  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall

>> prediction for VHA224 is nuc

>VHA224F.Seq
AATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGAT
AAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAA
CTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCA
GTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGG
GAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGAT
TTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTA
GGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACTCT
GTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAATTC
CATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGATTA
TTTATTGCTCCNNNNNNNNNN

>VHA224Z.Seq
NNNNNNNNNNTGATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAA
AATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCT
ATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTA
TTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGT
TAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTT
TGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACC
TTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCG
TTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTC
TCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTG
GTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCG
ATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAA
AACTTTTACATTAATTTCTTTTTTTAATATAAAATAAAAAAAAATAATATTATTTAAAA

>VHA224P.Seq
AATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGAT
AAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAA
CTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCA
GTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGG
GAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGAT
TTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTA
GGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACTCT
GTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAATTC
CATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGATTA
TTTATTGCTCC----------TGATAGTATTACCACTGATCATATTTCACCAGCCGGTAA
CATCAATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGA
TTTTAACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGC
CAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACC
AACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCA
ACTCATTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAA
AGGTCCATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAG
ATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTC
TACTTTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCT
CATTAAAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTAT
CCTTCGTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTA
TGTTTTAAGAAAACTTTTACATTAATTTCTTTTTTTAATATAAAATAAAAAAAAATAATA
TTATTTAAAA