>VHA440 (VHA440Q) /CSM/VH/VHA4-B/VHA440Q.Seq.d/
GTTTATAATTACAAATAACTATTTTTATATTTTTATATTTATTATTTTTTTTAATATATT
TTTTTATTTTCATTTTTTTGTATAAACTTACATAAAAAGATAAAAAGAAAAAAATGTCTA
AACCAATGGATGTAGAACAAGAACCAAAAATTGATGATGCTTTATATAGCAGACAACTTT
ATGCATTATCTCATGAAACAATGAAAAAAACTACATCAACTTCAGTTTTAGTTGTAGGTT
TACAAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTGTTAAATCTGTTA
CATTGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAATTTTATTTTTCAC
CAGAACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAGTTGTAGATTTAA
ATAATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAATTCTTAAAGAAAT
TCAATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAGTGAATGAATTTT
GTCATGCAAATAAAATTCATTTCATTAGTGTTGAAACCAGAGGTGTTTTTGGTCAATTAT
TCAATGATTTTGGTGAACAATTTACAATCACCGATACAAATXXXXXXXXXXAGGTTAAGA
TTCAAACCTCTGAAAATGAACCAGCTCCATCTTCAAACACTCAACAAGCTGGTGGTGATG
CTGAAGATGATCAATGTGATACCATTTTATCACAACTTCCACAACCAAGTGAAATGGCTG
GTTACAAAATTAACTCTATTCAATTTGAAAAGGATGATGATACCAATCATCATATCGATT
TCATTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATAAAC
ATAAGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAG
TTGCTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAAT
ATAAGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCG
CCCCAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTG
ATGGTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATA
TTTCAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAACTA
AAGAGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAACCAT
TACCAAAAGATAAGAAATATTTATTACTNAAATTTGTTGCAA

l*lqitififlyllffliyffififlyklt*KDKKKKMSKPMDVEQEPKIDDALYSRQLY
ALSHETMKKTTSTSVLVVGLQGLGIEIVKDLSLAGVKSVTLYDKELVEIKDLSSQFYFSP
EQVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEFLKKFNVVVLANQPLALQLKVNEFC
HANKIHFISVETRGVFGQLFNDFGEQFTITDTN---

---VKIQTSENEPAPSSNTQQAGGDAEDDQCDTILSQLPQPSEMAGYKINSIQFEKDDDT
NHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTTAVVAGFVCIELIKVIQN
KALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRFDVDGDITLKEFLDLFEK
KHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSKKPLPKDKKYLLLKFVA

Frame A:
vynyk*lflyfyiyyff*yiflfsffcinlhkkikrkkclnqwm*nknqklmmlyiadnf
mhylmkq*kklhqlqf*l*vykvlvlkllki*v*qvlnllhcmikn*lklkicllnfifh
qnklvklveqmhvskkl*i*iimlelmyimen*vmns*rnsml*y*pinh*hynsk*mnf
vmqikfislvlkpevflvnysmilvnnlqspiq---

---rlrfkplkmnqlhlqtlnklvvmlkminvipfyhnfhnqvkwlvtkltlfnlkrmmi
piiisislplpqi*elptmqsvqlinirpkvspvksfql*lpllp*llvsfvsn*lklfk
iklwrnirapl*i*vshslvslnqlppqrtkfvkaglghfgivsmlmvislsrsf*islk
rnmvwifqcslvklhfymlsslirklkrkd*r*kshnsmkpsqrnhyqkirniyyxnllq

Frame B:
fiitnnyfyififiiffniffyfhffv*tyikr*keknv*tngcrtrtkn**cfi*qttl
ciis*nneknyinfsfscrftrswy*nc*rfkfsrc*icyiv**ris*n*rfvfsilfft
rtsw*sw*srcmfpkscrfk*lc*n*ct*wrik**ilkeiqccsisqstisittqse*il
sck*nsfh*c*nqrcfwsiiq*fw*tiynhryk---

---g*dsnl*k*tssifkhstsww*c*r*sm*yhfittsttk*ngwlqn*lysi*kg**y
qssyrfhyrylkfksyqlcnqss**t*dqryrr*nhssfsyhycrscwfrlyrid*sysk
*sfgei*ehlyefrypilwfr*tncrpkeqns*rldldtlgsfrc*w*yhsqgvfrsl*k
etwfgyfnall*sytficslh**en*rgkikdenlttl*nplketitkr*eifitxicc

Frame C:
l*lqitififlyllffliyffififlyklt*KDKKKKMSKPMDVEQEPKIDDALYSRQLY
ALSHETMKKTTSTSVLVVGLQGLGIEIVKDLSLAGVKSVTLYDKELVEIKDLSSQFYFSP
EQVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEFLKKFNVVVLANQPLALQLKVNEFC
HANKIHFISVETRGVFGQLFNDFGEQFTITDTN---

---VKIQTSENEPAPSSNTQQAGGDAEDDQCDTILSQLPQPSEMAGYKINSIQFEKDDDT
NHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTTAVVAGFVCIELIKVIQN
KALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRFDVDGDITLKEFLDLFEK
KHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSKKPLPKDKKYLLLKFVA

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA440 (VHA440Q) /CSM/VH/VHA4-B/VHA440Q.Seq.d/                       2367   0.0
VHN723 (VHN723Q) /CSM/VH/VHN7-A/VHN723Q.Seq.d/                       1403   0.0
VHB744 (VHB744Q) /CSM/VH/VHB7-B/VHB744Q.Seq.d/                       1403   0.0
VHA239 (VHA239Q) /CSM/VH/VHA2-B/VHA239Q.Seq.d/                       1403   0.0
SHC572 (SHC572Q) /CSM/SH/SHC5-C/SHC572Q.Seq.d/                       1403   0.0
CHS124 (CHS124Q) /CSM/CH/CHS1-A/CHS124Q.Seq.d/                       1403   0.0
AHD109 (AHD109Q) /CSM/AH/AHD1-A/AHD109Q.Seq.d/                       1403   0.0
SHC317 (SHC317Q) /CSM/SH/SHC3-A/SHC317Q.Seq.d/                       1402   0.0
VHA328 (VHA328Q) /CSM/VH/VHA3-B/VHA328Q.Seq.d/                       1398   0.0
VHA132 (VHA132Q) /CSM/VH/VHA1-B/VHA132Q.Seq.d/                       1398   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AZ930776|AZ930776.1 474.dhz58e03.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomycesunisporus genomic clone 474.dhz58e03.s1, genomic survey sequence.    68   1e-12  3
CB933531|CB933531.1 ri31b11.y1 Meloidogyne chitwoodi egg pAMP1 v1 Meloidogyne chitwoodi cDNA 5' similar to TR:Q27481 Q27481 C47E12.5 PROTEIN. [1] ;, mRNA sequence.    64   5e-12  2
ED188983|ED188983.1 AUAC-aav62e08.g1 Ascaris suum whole genome shotgun library (PMAJ_4 GSS) Ascaris suum genomic, genomic survey sequence.    60   2e-04  1
DR174755|DR174755.1 HR0003_N01:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   7e-04  1
DR174741|DR174741.1 HR0003_M08:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   7e-04  1
DR174736|DR174736.1 HR0003_M02:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   7e-04  1
AC134210|AC134210.1 Rattus norvegicus clone CH230-197N6, *** SEQUENCING IN PROGRESS ***, 60 unordered pieces.    58   7e-04  1
AC108277|AC108277.6 Rattus norvegicus clone CH230-27E16, *** SEQUENCING IN PROGRESS ***, 8 unordered pieces.    58   7e-04  1
BZ303839|BZ303839.1 KD2618.q1 Kluyveromyces delphensis Random Genomic Library Kluyveromyces delphensis genomic clone KD2618, DNA sequence.    48   0.002  2
EE674138| ubiquitin activating enzyme [Drosophila melanogaster], mRNA sequence.    56   0.003  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55C16) RecName: Full=Ubiquitin-like modifier-activating enzyme...   439   e-121
EF468345_1(EF468345|pid:none) Pisum sativum ubiquitin activating...   240   1e-61
AE017341_129(AE017341|pid:none) Cryptococcus neoformans var. neo...   232   2e-59
FN392321_874(FN392321|pid:none) Pichia pastoris GS115 chromosome...   225   3e-57
CR382138_755(CR382138|pid:none) Debaryomyces hansenii strain CBS...   223   1e-56
BX908809_46(BX908809|pid:none) Neurospora crassa DNA linkage gro...   221   5e-56
AE016819_666(AE016819|pid:none) Ashbya gossypii (= Eremothecium ...   219   1e-55
CR382122_592(CR382122|pid:none) Kluyveromyces lactis strain NRRL...   219   2e-55
(P22515) RecName: Full=Ubiquitin-activating enzyme E1 1;  &S3804...   219   2e-55
CP000496_820(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...   218   3e-55

 psg: 0.50  gvh: 0.50  alm: 0.27  top: 0.43  tms: 0.07  mit: 0.21  mip: 0.00
 nuc: 0.13  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  8.0 %: peroxisomal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHA440 is cyt

>VHA440F.Seq
GTTTATAATTACAAATAACTATTTTTATATTTTTATATTTATTATTTTTTTTAATATATT
TTTTTATTTTCATTTTTTTGTATAAACTTACATAAAAAGATAAAAAGAAAAAAATGTCTA
AACCAATGGATGTAGAACAAGAACCAAAAATTGATGATGCTTTATATAGCAGACAACTTT
ATGCATTATCTCATGAAACAATGAAAAAAACTACATCAACTTCAGTTTTAGTTGTAGGTT
TACAAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTGTTAAATCTGTTA
CATTGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAATTTTATTTTTCAC
CAGAACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAGTTGTAGATTTAA
ATAATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAATTCTTAAAGAAAT
TCAATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAGTGAATGAATTTT
GTCATGCAAATAAAATTCATTTCATTAGTGTTGAAACCAGAGGTGTTTTTGGTCAATTAT
TCAATGATTTTGGTGAACAATTTACAATCACCGATACAAATNNNNNNNNNN

>VHA440Z.Seq
NNNNNNNNNNAGGTTAAGATTCAAACCTCTGAAAATGAACCAGCTCCATCTTCAAACACT
CAACAAGCTGGTGGTGATGCTGAAGATGATCAATGTGATACCATTTTATCACAACTTCCA
CAACCAAGTGAAATGGCTGGTTACAAAATTAACTCTATTCAATTTGAAAAGGATGATGAT
ACCAATCATCATATCGATTTCATTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCA
ATCAGTCCAGCTGATAAACATAAGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTA
GTTACCACTACTGCCGTAGTTGCTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAA
AATAAAGCTTTGGAGAAATATAAGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGT
TTCGTTGAACCAATTGCCGCCCCAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTT
TGGGATCGTTTCGATGTTGATGGTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAA
AAGAAACATGGTTTGGATATTTCAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTC
TTCACTGATAAGAAAACTAAAGAGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAA
ACCCTCTCAAAGAAACCATTACCAAAAGATAAGAAATATTTATTACTNAAATTTGTTGCA
A

>VHA440P.Seq
GTTTATAATTACAAATAACTATTTTTATATTTTTATATTTATTATTTTTTTTAATATATT
TTTTTATTTTCATTTTTTTGTATAAACTTACATAAAAAGATAAAAAGAAAAAAATGTCTA
AACCAATGGATGTAGAACAAGAACCAAAAATTGATGATGCTTTATATAGCAGACAACTTT
ATGCATTATCTCATGAAACAATGAAAAAAACTACATCAACTTCAGTTTTAGTTGTAGGTT
TACAAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTGTTAAATCTGTTA
CATTGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAATTTTATTTTTCAC
CAGAACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAGTTGTAGATTTAA
ATAATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAATTCTTAAAGAAAT
TCAATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAGTGAATGAATTTT
GTCATGCAAATAAAATTCATTTCATTAGTGTTGAAACCAGAGGTGTTTTTGGTCAATTAT
TCAATGATTTTGGTGAACAATTTACAATCACCGATACAAAT----------AGGTTAAGA
TTCAAACCTCTGAAAATGAACCAGCTCCATCTTCAAACACTCAACAAGCTGGTGGTGATG
CTGAAGATGATCAATGTGATACCATTTTATCACAACTTCCACAACCAAGTGAAATGGCTG
GTTACAAAATTAACTCTATTCAATTTGAAAAGGATGATGATACCAATCATCATATCGATT
TCATTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATAAAC
ATAAGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAG
TTGCTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAAT
ATAAGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCG
CCCCAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTG
ATGGTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATA
TTTCAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAACTA
AAGAGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAACCAT
TACCAAAAGATAAGAAATATTTATTACTNAAATTTGTTGCAA