>VHB467 (VHB467Q) /CSM/VH/VHB4-C/VHB467Q.Seq.d/
XXXXXXXXXXTTAGCAAATACTTATTCAGGTTATATTGCAACATTTGAAGAGTTTCAAGT
TCAAAGATATGAAGGTGCATCAACCGTTTTTGGACCTCATACTTTAGGAGCATATCAACA
AGAATTTGCAAATCTTGCACAATCAATTGTAGATGGTAGTCAAGCTGATCCAGGAACATT
CCCAAGAAATATGTCTGGTCATACACCATTCTTTTTACCACCAGTCATTGTTGATGTAGC
TCCAAAATTCGACGATTTCGGTGATATCTATACTGATGTCTCTACAACTACACCATATTC
AATCAATCAAACTGTTACCGTCATTTTCTATGGTGCCAATCTTAGAAATAATTTCATGAC
TGAATCTTCTTTCCTCACTGTTGATCAATTACAATCTAATGGTCAATGGACTACAATTTT
AAATGATGGTGATTGGGATACAAAATTATATTGGAAAATGCATGATTTAGGTTTTTCACT
CATCACTGTTGATTGGACAATCTCTCCAATCACTCAACCAGGTACTTATAGAATAACTCA
TAGTGGTTATGCTAAGAAAAATCCATTCTCTGATAATTTAACTTTTTATCAAGGTATTTC
AAGTAATTTTAATGTTCAATAAAAATTAA

---ANTYSGYIATFEEFQVQRYEGASTVFGPHTLGAYQQEFANLAQSIVDGSQADPGTFP
RNMSGHTPFFLPPVIVDVAPKFDDFGDIYTDVSTTTPYSINQTVTVIFYGANLRNNFMTE
SSFLTVDQLQSNGQWTTILNDGDWDTKLYWKMHDLGFSLITVDWTISPITQPGTYRITHS
GYAKKNPFSDNLTFYQGISSNFNVQ*kl

Frame A:
---*qiliqvilqhlksfkfkdmkvhqpfldlil*ehinknlqilhnql*mvvkliqehs
qeiclvihhsfyhqsllm*lqnstisvisilmslqlhhiqsikllpsfsmvpileiis*l
nllssllinynlmvnglqf*mmvigiqnyigkcmi*vfhsslligqslqslnqvlie*li
vvmlrkihslii*lfikvfqvilmfnkn*


Frame B:
---skylfrlycni*rvssski*rcinrfwtsyfrsistricksctincrw*ss*srnip
kkyvwsytilfttshc*csskirrfr*yly*clynytifnqsncyrhflwcqs*k*fhd*
iffphc*siti*wsmdynfk*w*lgykiilena*frffthhc*ldnlsnhstryl*nns*
wlc*eksil**fnflsryfk*f*csiki


Frame C:
---ANTYSGYIATFEEFQVQRYEGASTVFGPHTLGAYQQEFANLAQSIVDGSQADPGTFP
RNMSGHTPFFLPPVIVDVAPKFDDFGDIYTDVSTTTPYSINQTVTVIFYGANLRNNFMTE
SSFLTVDQLQSNGQWTTILNDGDWDTKLYWKMHDLGFSLITVDWTISPITQPGTYRITHS
GYAKKNPFSDNLTFYQGISSNFNVQ*kl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD672 (VSD672Q) /CSM/VS/VSD6-C/VSD672Q.Seq.d/                       1227   0.0
VHP543 (VHP543Q) /CSM/VH/VHP5-B/VHP543Q.Seq.d/                       1227   0.0
VHK193 (VHK193Q) /CSM/VH/VHK1-D/VHK193Q.Seq.d/                       1227   0.0
VHJ723 (VHJ723Q) /CSM/VH/VHJ7-A/VHJ723Q.Seq.d/                       1227   0.0
VHI396 (VHI396Q) /CSM/VH/VHI3-D/VHI396Q.Seq.d/                       1227   0.0
VHC573 (VHC573Q) /CSM/VH/VHC5-D/VHC573Q.Seq.d/                       1227   0.0
VHB761 (VHB761Q) /CSM/VH/VHB7-C/VHB761Q.Seq.d/                       1227   0.0
VHB467 (VHB467Q) /CSM/VH/VHB4-C/VHB467Q.Seq.d/                       1227   0.0
VFM574 (VFM574Q) /CSM/VF/VFM5-D/VFM574Q.Seq.d/                       1227   0.0
SFK788 (SFK788Q) /CSM/SF/SFK7-D/SFK788Q.Seq.d/                       1227   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AB121061|AB121061.1 Dictyostelium discoideum dcd2A mRNA for ceramidase, complete cds.  1227   0.0    1
AE000783|AE000783.1 Borrelia burgdorferi B31, complete genome.     40   1.7    1
AB011477|AB011477.1 Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MHK7.    40   1.7    1
AC079933|AC079933.3 Trypanosoma brucei chromosome 4 clone RPCI93-1H19, complete sequence.    40   1.7    1
AC008346|AC008346.6 Drosophila melanogaster clone BACR45K04, complete sequence.    40   1.7    1
AC008097|AC008097.5 Drosophila melanogaster clone BACR20D06, complete sequence.    40   1.7    1
AB053222|AB053222.1 Homo sapiens gene for N-acetylglucosaminyltransferase IVa, partial cds.    40   1.7    1
AC084677|AC084677.1 Caenorhabditis briggsae cosmid G47L22, complete sequence.    36   2.0    2
AC146200|AC146200.2 Pan troglodytes BAC clone RP43-34F23 from chromosome 7, complete sequence.    34   3.3    3
AC009821|AC009821.3 Homo sapiens chromosome 15 clone RP11-27P20 map 15, WORKING DRAFT SEQUENCE, 10 unordered pieces.    38   6.8    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U82513_1(U82513|pid:none) Dictyostelium discoideum random slug c...   424   e-118
(Q55G11) RecName: Full=Neutral ceramidase B;          Short=N-CD...   309   5e-83
AC022464_28(AC022464|pid:none) Genomic sequence for Arabidopsis ...   149   5e-35
BC167651_1(BC167651|pid:none) Xenopus tropicalis hypothetical pr...   146   5e-34
CP000777_2353(CP000777|pid:none) Leptospira biflexa serovar Pato...   146   5e-34
AP002819_24(AP002819|pid:none) Oryza sativa Japonica Group genom...   144   3e-33
EU200974_1(EU200974|pid:none) Triticum aestivum neutral ceramida...   144   3e-33
AK099625_1(AK099625|pid:none) Oryza sativa Japonica Group cDNA c...   144   3e-33
(Q304B9) RecName: Full=Neutral ceramidase;          Short=N-CDas...   141   1e-32
AK301784_1(AK301784|pid:none) Homo sapiens cDNA FLJ51366 complet...   141   2e-32

 psg: 0.86  gvh: 0.57  alm: 0.45  top: 0.33  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: extracellular, including cell wall
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system

>> prediction for VHB467 is cyt

>VHB467Z.Seq
NNNNNNNNNNTTAGCAAATACTTATTCAGGTTATATTGCAACATTTGAAGAGTTTCAAGT
TCAAAGATATGAAGGTGCATCAACCGTTTTTGGACCTCATACTTTAGGAGCATATCAACA
AGAATTTGCAAATCTTGCACAATCAATTGTAGATGGTAGTCAAGCTGATCCAGGAACATT
CCCAAGAAATATGTCTGGTCATACACCATTCTTTTTACCACCAGTCATTGTTGATGTAGC
TCCAAAATTCGACGATTTCGGTGATATCTATACTGATGTCTCTACAACTACACCATATTC
AATCAATCAAACTGTTACCGTCATTTTCTATGGTGCCAATCTTAGAAATAATTTCATGAC
TGAATCTTCTTTCCTCACTGTTGATCAATTACAATCTAATGGTCAATGGACTACAATTTT
AAATGATGGTGATTGGGATACAAAATTATATTGGAAAATGCATGATTTAGGTTTTTCACT
CATCACTGTTGATTGGACAATCTCTCCAATCACTCAACCAGGTACTTATAGAATAACTCA
TAGTGGTTATGCTAAGAAAAATCCATTCTCTGATAATTTAACTTTTTATCAAGGTATTTC
AAGTAATTTTAATGTTCAATAAAAATTAA