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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFN550
Library AF
(Link to library)
Clone ID AFN550
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10672-1
Original site URL
Representative seq. ID AFN550P
(Link to Original site)
Representative DNA sequence 
>AFN550 (AFN550Q) /CSM/AF/AFN5-C/AFN550Q.Seq.d/
ATTATTAGTTAATTTTCATTTGTAAATATAAAAAAAAAATGGAATCATTAAAAAACAATG
GTGAAAAAATTGAAAATTTTTTAAATCAATTAAAAAATCTTCAATCAAATGTAGACAAAG
ATTTTAAAGAATTTTCAAATTCTCCAAATACTTTAAATGCTGAAAAAGATGGGGAGTTTC
TTGATAAACATGATGAAGAATTATACAACAAACAAATAATTCGTTCAAGACTAGAAGATA
AAATTTCAAATTTAAGTTCTTCTATGATTAATGATATTGGGAATGATTTTCAAACTGGTT
TTCAAACAATTCTATACAATTTACCAGGTGTTGGTCCAGTTTTCCAAATGATCTTTAATA
TTATGGGTAATGGTATAAATTTAGAAAAATTTTACCAAGAAATCATTACACAAGTAGAAC
AAATGATTAAAAAATCATTAGAAGATTATTATAAAAACCAATGTAATACAATTTTTAAAA
ATTTAGGAAAAGCATGTGATCAACATAAAGATCTAACTCAAAAATGGTATGATAAAAATG
GTATCAAATCAATGAACCATTXXXXXXXXXXTCTCTTCAAGAAGGTTATTGGATAACATA
TCCACCACCAATTTATAAGGTTTGGGTTCCACCACAACAAGCATCGTGGGTATCTCCTCC
TGCCTCGGAATTGGCACATAAATTCTTTTTGGCCCATTCTGACAATTATCCTCAAGGTGG
TGATCTAATTTATATTAAAAAAGATGGAGTTTATGAACTTAGTCCAAATAAAATAACTCA
TGCCCTTGAAGTTTATTATAGCTCTGGGGGTGGATATGGTGTTAATGACTATCCAAGTTT
TCGTTATGGTGGAAAACCAATCATACCTCTTCTCGCTCCAGTTGGTAATGCTGTGTTTAC
AATGTTAAATCCAAATCGTTATAAAAGAACCTTTAAAATTAGAATTGTTCATGTCATTGT
TAGAGAAGCCAAATGGAATTTAGATTGCTATGATAATCAAGTTGGGGAAGCAGGTTCATT
TAACCAAAATTTTGTTTTCACTTCAACTAATATAGTTTCTGACCCAATTTGTGGGAATAT
TGGAACTGTTGGTATCACTGAAATTCCAGGACCATTTACAACTGATAAAAAGTATATTAG
ATTAACATGCATTCGTAAAGTAAATTTCCCAGCATCAAAAAATGAAGGATATGCCGTTGG
ATCTCGTATTTTATCAGTACAATTAATNATTTATAACCACTTCAATAAAATNATTTTCAT
TTAAAATANT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
y*LIFICKYKKKMESLKNNGEKIENFLNQLKNLQSNVDKDFKEFSNSPNTLNAEKDGEFL
DKHDEELYNKQIIRSRLEDKISNLSSSMINDIGNDFQTGFQTILYNLPGVGPVFQMIFNI
MGNGINLEKFYQEIITQVEQMIKKSLEDYYKNQCNTIFKNLGKACDQHKDLTQKWYDKNG
IKSMNH---

---SLQEGYWITYPPPIYKVWVPPQQASWVSPPASELAHKFFLAHSDNYPQGGDLIYIKK
DGVYELSPNKITHALEVYYSSGGGYGVNDYPSFRYGGKPIIPLLAPVGNAVFTMLNPNRY
KRTFKIRIVHVIVREAKWNLDCYDNQVGEAGSFNQNFVFTSTNIVSDPICGNIGTVGITE
IPGPFTTDKKYIRLTCIRKVNFPASKNEGYAVGSRILSVQLXIYNHFNKXIFI*nx
Translated Amino Acid sequence (All Frames) 
Frame A:
iis*fsfvnikkkwnh*ktmvkklkif*in*kifnqm*tkilknfqilqil*mlkkmgsf
linmmknyttnk*fvqd*kikfqi*vll*lmilgmifklvfkqfytiyqvlvqfsk*sli
lwvmv*i*knftkkslhk*nk*lknh*kiiiktnviqflki*ekhviniki*lkngmikm
vsnq*ti---

---SLQEGYWITYPPPIYKVWVPPQQASWVSPPASELAHKFFLAHSDNYPQGGDLIYIKK
DGVYELSPNKITHALEVYYSSGGGYGVNDYPSFRYGGKPIIPLLAPVGNAVFTMLNPNRY
KRTFKIRIVHVIVREAKWNLDCYDNQVGEAGSFNQNFVFTSTNIVSDPICGNIGTVGITE
IPGPFTTDKKYIRLTCIRKVNFPASKNEGYAVGSRILSVQLXIYNHFNKXIFI*nx

Frame B:
llvnfhl*i*kkngiikkqw*kn*kffksikkssikcrqrf*rifkfskyfkc*krwgvs
**t**riiqqtnnsfktrr*nfkfkffyd**ywe*fsnwfsnnsiqftrcwssfpndl*y
yg*wykfrkilprnhytsrtnd*kiirrll*kpm*ynf*kfrksm*st*rsnskmv**kw
yqinep---

---lfkkvig*hihhqfirfgfhhnkhrgylllprnwhinsfwpiltiilkvvi*filkk
mefmnlvqik*lmplkfiialgvdmvlmtiqvfvmvenqsylfslqlvmlclqc*iqivi
keplklelfmsllekpngi*iamiiklgkqvhltkilfslqli*fltqfvgilellvslk
fqdhlqliksild*hafvk*isqhqkmkdmpldlvfyqyn*xfittsikxfsfki

Frame C:
y*LIFICKYKKKMESLKNNGEKIENFLNQLKNLQSNVDKDFKEFSNSPNTLNAEKDGEFL
DKHDEELYNKQIIRSRLEDKISNLSSSMINDIGNDFQTGFQTILYNLPGVGPVFQMIFNI
MGNGINLEKFYQEIITQVEQMIKKSLEDYYKNQCNTIFKNLGKACDQHKDLTQKWYDKNG
IKSMNH---

---ssrrlldnisttnl*glgstttsivgissclgigt*ilfgpf*qlssrw*snly*kr
wsl*t*sk*nnscp*sll*lwgwiwc**lskfslwwktnhtssrssw*ccvynvksksl*
knl*n*ncschc*rsqmefrll**sswgsrfi*pkfcfhfn*ysf*pnlweywncwyh*n
srtiyn**kvy*inmhs*skfpsikk*ricrwisyfistinxl*plq*nxfhlkx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFN550 (AFN550Q) /CSM/AF/AFN5-C/AFN550Q.Seq.d/                       2224   0.0
AFK522 (AFK522Q) /CSM/AF/AFK5-A/AFK522Q.Seq.d/                       2216   0.0
AFN662 (AFN662Q) /CSM/AF/AFN6-C/AFN662Q.Seq.d/                       2189   0.0
AFC537 (AFC537Q) /CSM/AF/AFC5-B/AFC537Q.Seq.d/                       1364   0.0
VFI666 (VFI666Q) /CSM/VF/VFI6-C/VFI666Q.Seq.d/                       1362   0.0
AFH818 (AFH818Q) /CSM/AF/AFH8-A/AFH818Q.Seq.d/                       1362   0.0
AFE435 (AFE435Q) /CSM/AF/AFE4-B/AFE435Q.Seq.d/                       1362   0.0
CHD481 (CHD481Q) /CSM/CH/CHD4-D/CHD481Q.Seq.d/                       1360   0.0
AFO392 (AFO392Q) /CSM/AF/AFO3-D/AFO392Q.Seq.d/                       1360   0.0
AFN784 (AFN784Q) /CSM/AF/AFN7-D/AFN784Q.Seq.d/                       1356   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117072|AC117072.3 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.  1209   0.0    13
M15968|M15968.1 D.discoideum M3R gene, exons 1-3.                 541   0.0    3
M15967|M15967.1 D.discoideum M3L gene, exons 1-3.                 236   e-143  3
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    38   7e-09  21
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    34   5e-08  19
BX842644|BX842644.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 3/4.    34   5e-08  19
AF303741|AF303741.1 Chilo iridescent virus complete genome.        32   2e-07  18
AB063521|AB063521.2 Wigglesworthia brevipalpis DNA, complete genome, section 1/2.    34   1e-06  19
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    38   1e-06  24
AJ010592|AJ010592.2 Guillardia theta DNA for complete sequence of nucleomorph chromosome 2.    34   4e-06  17
dna update 2004. 8.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P11872) RecName: Full=cAMP-regulated M3R protein; AltName: Full...   435   e-120
AC117072_17(AC117072|pid:none) Dictyostelium discoideum chromoso...   302   1e-80
AE014187_536(AE014187|pid:none) Plasmodium falciparum 3D7 chromo...    48   8e-04
AE014186_384(AE014186|pid:none) Plasmodium falciparum 3D7 chromo...    45   0.007
CR380952_40(CR380952|pid:none) Candida glabrata strain CBS138 ch...    43   0.020
EF158087_1(EF158087|pid:none) Plasmodium falciparum erythrocyte ...    41   0.075
CP000879_149(CP000879|pid:none) Petrotoga mobilis SJ95, complete...    40   0.17
(O51267) RecName: Full=Leucyl-tRNA synthetase;          EC=6.1.1...    40   0.22
CP001205_240(CP001205|pid:none) Borrelia burgdorferi ZS7, comple...    40   0.22
AF128451_1(AF128451|pid:none) Borrelia turicatae plasmid-encoded...    40   0.22
protein update 2009. 4.25
PSORT 

 psg: 0.56  gvh: 0.29  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.13  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum

>> prediction for AFN550 is nuc
5' end seq. ID AFN550F
5' end seq. 
>AFN550F.Seq
ATTATTAGTTAATTTTCATTTGTAAATATAAAAAAAAAATGGAATCATTAAAAAACAATG
GTGAAAAAATTGAAAATTTTTTAAATCAATTAAAAAATCTTCAATCAAATGTAGACAAAG
ATTTTAAAGAATTTTCAAATTCTCCAAATACTTTAAATGCTGAAAAAGATGGGGAGTTTC
TTGATAAACATGATGAAGAATTATACAACAAACAAATAATTCGTTCAAGACTAGAAGATA
AAATTTCAAATTTAAGTTCTTCTATGATTAATGATATTGGGAATGATTTTCAAACTGGTT
TTCAAACAATTCTATACAATTTACCAGGTGTTGGTCCAGTTTTCCAAATGATCTTTAATA
TTATGGGTAATGGTATAAATTTAGAAAAATTTTACCAAGAAATCATTACACAAGTAGAAC
AAATGATTAAAAAATCATTAGAAGATTATTATAAAAACCAATGTAATACAATTTTTAAAA
ATTTAGGAAAAGCATGTGATCAACATAAAGATCTAACTCAAAAATGGTATGATAAAAATG
GTATCAAATCAATGAACCATT----------
Length of 5' end seq. 561
3' end seq. ID AFN550Z
3' end seq. 
>AFN550Z.Seq
----------TCTCTTCAAGAAGGTTATTGGATAACATATCCACCACCAATTTATAAGGT
TTGGGTTCCACCACAACAAGCATCGTGGGTATCTCCTCCTGCCTCGGAATTGGCACATAA
ATTCTTTTTGGCCCATTCTGACAATTATCCTCAAGGTGGTGATCTAATTTATATTAAAAA
AGATGGAGTTTATGAACTTAGTCCAAATAAAATAACTCATGCCCTTGAAGTTTATTATAG
CTCTGGGGGTGGATATGGTGTTAATGACTATCCAAGTTTTCGTTATGGTGGAAAACCAAT
CATACCTCTTCTCGCTCCAGTTGGTAATGCTGTGTTTACAATGTTAAATCCAAATCGTTA
TAAAAGAACCTTTAAAATTAGAATTGTTCATGTCATTGTTAGAGAAGCCAAATGGAATTT
AGATTGCTATGATAATCAAGTTGGGGAAGCAGGTTCATTTAACCAAAATTTTGTTTTCAC
TTCAACTAATATAGTTTCTGACCCAATTTGTGGGAATATTGGAACTGTTGGTATCACTGA
AATTCCAGGACCATTTACAACTGATAAAAAGTATATTAGATTAACATGCATTCGTAAAGT
AAATTTCCCAGCATCAAAAAATGAAGGATATGCCGTTGGATCTCGTATTTTATCAGTACA
ATTAATNATTTATAACCACTTCAATAAAATNATTTTCATTTAAAATANT
Length of 3' end seq. 699
Connected seq. ID AFN550P
Connected seq. 
>AFN550P.Seq
ATTATTAGTTAATTTTCATTTGTAAATATAAAAAAAAAATGGAATCATTAAAAAACAATG
GTGAAAAAATTGAAAATTTTTTAAATCAATTAAAAAATCTTCAATCAAATGTAGACAAAG
ATTTTAAAGAATTTTCAAATTCTCCAAATACTTTAAATGCTGAAAAAGATGGGGAGTTTC
TTGATAAACATGATGAAGAATTATACAACAAACAAATAATTCGTTCAAGACTAGAAGATA
AAATTTCAAATTTAAGTTCTTCTATGATTAATGATATTGGGAATGATTTTCAAACTGGTT
TTCAAACAATTCTATACAATTTACCAGGTGTTGGTCCAGTTTTCCAAATGATCTTTAATA
TTATGGGTAATGGTATAAATTTAGAAAAATTTTACCAAGAAATCATTACACAAGTAGAAC
AAATGATTAAAAAATCATTAGAAGATTATTATAAAAACCAATGTAATACAATTTTTAAAA
ATTTAGGAAAAGCATGTGATCAACATAAAGATCTAACTCAAAAATGGTATGATAAAAATG
GTATCAAATCAATGAACCATT----------TCTCTTCAAGAAGGTTATTGGATAACATA
TCCACCACCAATTTATAAGGTTTGGGTTCCACCACAACAAGCATCGTGGGTATCTCCTCC
TGCCTCGGAATTGGCACATAAATTCTTTTTGGCCCATTCTGACAATTATCCTCAAGGTGG
TGATCTAATTTATATTAAAAAAGATGGAGTTTATGAACTTAGTCCAAATAAAATAACTCA
TGCCCTTGAAGTTTATTATAGCTCTGGGGGTGGATATGGTGTTAATGACTATCCAAGTTT
TCGTTATGGTGGAAAACCAATCATACCTCTTCTCGCTCCAGTTGGTAATGCTGTGTTTAC
AATGTTAAATCCAAATCGTTATAAAAGAACCTTTAAAATTAGAATTGTTCATGTCATTGT
TAGAGAAGCCAAATGGAATTTAGATTGCTATGATAATCAAGTTGGGGAAGCAGGTTCATT
TAACCAAAATTTTGTTTTCACTTCAACTAATATAGTTTCTGACCCAATTTGTGGGAATAT
TGGAACTGTTGGTATCACTGAAATTCCAGGACCATTTACAACTGATAAAAAGTATATTAG
ATTAACATGCATTCGTAAAGTAAATTTCCCAGCATCAAAAAATGAAGGATATGCCGTTGG
ATCTCGTATTTTATCAGTACAATTAATNATTTATAACCACTTCAATAAAATNATTTTCAT
TTAAAATANT
Length of connected seq. 1260
Full length Seq ID -
Full length Seq. -
Length of full length seq. -