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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE189
Library VH
(Link to library)
Clone ID VHE189
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16292-1|Contig-U16400-1
Original site URL
Representative seq. ID VHE189P
(Link to Original site)
Representative DNA sequence 
>VHE189 (VHE189Q) /CSM/VH/VHE1-D/VHE189Q.Seq.d/
AGGTGTGTTCACAAAAATAACAACAACACTAAAAAAAGTCTTCATTGTTTTTTACTTTTT
TTTTTAATTTTTAATTTTTTATTTAATTTAAACAATATATAAATCAATTAAAATAAACAC
TCNCTCGATAGTGTAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAANNTTNTTAAAAAAAAAAXXXXXXXXXXAGGTCATGGGTGCCCATTGTGGTT
ATTTGGCTGCAATGAGTTCTTTAACCTCAGGTGCTGAACGTTCATATATAATGGAAAGAG
GTATCACATTGAATACACTAACCAAGGATTTGGAAATGTTTGTTGAACGTTTCAAACGTG
AACATAGAATTGGTCTAATTATTAAGAGTGAACTTGCTAGCAACACCTACTCAACTCATT
TCATTTATTCACTCTTCAAAGAAGAAGGTAAACATTTATTCGATGTCCGTGAATCTATAC
TCGGTCATTTACAACAAGGTGGTACTCCTTCTGCAATTGATCGTATCTTTTCAACTCGTT
TAATGAATCATTATTATCAATTCTTGGAAAACGATCTCAAAGAACATGGTCATCTTCAAA
TGAATGGTTGTATTGGTTTCATTGATGGTGGTATTCATTACACTCCAATGCAAGAAATGA
TCGAA
sequence update 2002.10.25
Translated Amino Acid sequence 
vcsqk*qqh*kksslfftfffnf*ffi*fkqyinqlk*tlxr*CKRKKKKKKKKKKKKKK
KKXXLKKK---

---VMGAHCGYLAAMSSLTSGAERSYIMERGITLNTLTKDLEMFVERFKREHRIGLIIKS
ELASNTYSTHFIYSLFKEEGKHLFDVRESILGHLQQGGTPSAIDRIFSTRLMNHYYQFLE
NDLKEHGHLQMNGCIGFIDGGIHYTPMQEMIE
Translated Amino Acid sequence (All Frames) 
Frame A:
rcvhknnnntkkslhcfllfflifnflfnlnni*in*nkhsldsvkekkkkkkkkkkkkk
kkkxx*kk---

---rswvpivviwlq*vl*pqvlnvhi*wkevsh*ih*priwkcllnvsnvnielv*llr
vnllatptqlisfihsskkkvniysmsvnlysviynkvvlllqlivsfqlv**iiiinsw
ktisknmvifk*mvvlvslmvvfitlqckk*s

Frame B:
gvftkitttlkkvfivfyfff*flifyli*tiyksikintxsiv*kkkkkkkkkkkkkkk
kkxxxkkk---

---ghgcplwlfgcneffnlrc*tfiyngkryhieytnqgfgnvc*tfqt*t*nwsny*e
*tc*qhllnsfhlftlqrrr*tfircp*iytrsfttrwysfcn*sylfnsfnesllsilg
krsqrtwsssnewlywfh*wwyslhsnarndr

Frame C:
vcsqk*qqh*kksslfftfffnf*ffi*fkqyinqlk*tlxr*CKRKKKKKKKKKKKKKK
KKXXLKKK---

---VMGAHCGYLAAMSSLTSGAERSYIMERGITLNTLTKDLEMFVERFKREHRIGLIIKS
ELASNTYSTHFIYSLFKEEGKHLFDVRESILGHLQQGGTPSAIDRIFSTRLMNHYYQFLE
NDLKEHGHLQMNGCIGFIDGGIHYTPMQEMIE
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD874 (VHD874Q) /CSM/VH/VHD8-D/VHD874Q.Seq.d/                        890   0.0
VHP708 (VHP708Q) /CSM/VH/VHP7-A/VHP708Q.Seq.d/                        890   0.0
VHP472 (VHP472Q) /CSM/VH/VHP4-C/VHP472Q.Seq.d/                        890   0.0
VHP463 (VHP463Q) /CSM/VH/VHP4-C/VHP463Q.Seq.d/                        890   0.0
VHO736 (VHO736Q) /CSM/VH/VHO7-B/VHO736Q.Seq.d/                        890   0.0
VHO650 (VHO650Q) /CSM/VH/VHO6-C/VHO650Q.Seq.d/                        890   0.0
VHN894 (VHN894Q) /CSM/VH/VHN8-D/VHN894Q.Seq.d/                        890   0.0
VHN560 (VHN560Q) /CSM/VH/VHN5-C/VHN560Q.Seq.d/                        890   0.0
VHM809 (VHM809Q) /CSM/VH/VHM8-A/VHM809Q.Seq.d/                        890   0.0
VHM459 (VHM459Q) /CSM/VH/VHM4-C/VHM459Q.Seq.d/                        890   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   890   0.0    3
X94340|X94340.1 D.discoideum mRNA for 6-phosphofructo-1-kinase.   890   0.0    1
X89039|X89039.1 D.discoideum mRNA for 6-phosphofructo-1-kinase.   890   0.0    1
AJ430522|AJ430522.1 Dictyostelium discoideum partial pfk gene for ATP:D-fructose  6-phosphate 1-phosphofructokinase, exon 1.    94   7e-34  2
AL355522|AL355522.11 Human DNA sequence from clone RP11-546O15 on chromosome 6.    46   0.21   4
DX414156|DX414156.1 SOYC248TH LargeInsertSoybeanGenLibBuild4 Glycine max genomic clone H12A17:Build4MTP4H23, genomic survey sequence.    48   0.32   1
AC148856|AC148856.2 Otolemur garnettii clone CH256-528G23, WORKING DRAFT SEQUENCE, 2 ordered pieces.    46   1.3    1
AC018412|AC018412.3 Homo sapiens clone RP11-17I8, WORKING DRAFT SEQUENCE, 12 unordered pieces.    46   1.3    1
CE635221|CE635221.1 tigr-gss-dog-17000366743799 Dog Library Canis familiaris genomic, genomic survey sequence.    46   1.3    1
BZ298846|BZ298846.1 CG4616.f1 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG4616, DNA sequence.    46   1.3    1
dna update 2007. 6.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P90521) RecName: Full=6-phosphofructokinase;          Short=Pho...   305   6e-82
CP001337_2617(CP001337|pid:none) Chloroflexus aggregans DSM 9485...   132   9e-30
BC081652_1(BC081652|pid:none) Danio rerio phosphofructokinase, m...   116   5e-25
(Q867C9) RecName: Full=6-phosphofructokinase, muscle type;      ...   113   6e-24
DQ480147_1(DQ480147|pid:none) Sus scrofa muscle 6-phosphofructok...   112   1e-23
BC077756_1(BC077756|pid:none) Xenopus laevis MGC79063 protein, m...   112   1e-23
AB303967_1(AB303967|pid:none) Macaca fascicularis PFKM mRNA for ...   111   2e-23
AY335614_1(AY335614|pid:none) Synthetic construct Homo sapiens p...   111   2e-23
CR858939_1(CR858939|pid:none) Pongo abelii mRNA; cDNA DKFZp468O1...   111   2e-23
(P08237) RecName: Full=6-phosphofructokinase, muscle type;      ...   111   2e-23
protein update 2009. 6.30
PSORT 

 psg: 0.03  gvh: 0.62  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.02
 nuc: 1.28  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: cytoplasmic

>> prediction for VHE189 is nuc
5' end seq. ID VHE189F
5' end seq. 
>VHE189F.Seq
AGGTGTGTTCACAAAAATAACAACAACACTAAAAAAAGTCTTCATTGTTTTTTACTTTTT
TTTTTAATTTTTAATTTTTTATTTAATTTAAACAATATATAAATCAATTAAAATAAACAC
TCNCTCGATAGTGTAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAANNTTNTTAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 216
3' end seq. ID VHE189Z
3' end seq. 
>VHE189Z.Seq
NNNNNNNNNNAGGTCATGGGTGCCCATTGTGGTTATTTGGCTGCAATGAGTTCTTTAACC
TCAGGTGCTGAACGTTCATATATAATGGAAAGAGGTATCACATTGAATACACTAACCAAG
GATTTGGAAATGTTTGTTGAACGTTTCAAACGTGAACATAGAATTGGTCTAATTATTAAG
AGTGAACTTGCTAGCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAAGAAGAA
GGTAAACATTTATTCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGTGGTACT
CCTTCTGCAATTGATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAATTCTTG
GAAAACGATCTCAAAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTCATTGAT
GGTGGTATTCATTACACTCCAATGCAAGAAATGATCGAA
Length of 3' end seq. 459
Connected seq. ID VHE189P
Connected seq. 
>VHE189P.Seq
AGGTGTGTTCACAAAAATAACAACAACACTAAAAAAAGTCTTCATTGTTTTTTACTTTTT
TTTTTAATTTTTAATTTTTTATTTAATTTAAACAATATATAAATCAATTAAAATAAACAC
TCNCTCGATAGTGTAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAANNTTNTTAAAAAAAAAA----------AGGTCATGGGTGCCCATTGTGGTT
ATTTGGCTGCAATGAGTTCTTTAACCTCAGGTGCTGAACGTTCATATATAATGGAAAGAG
GTATCACATTGAATACACTAACCAAGGATTTGGAAATGTTTGTTGAACGTTTCAAACGTG
AACATAGAATTGGTCTAATTATTAAGAGTGAACTTGCTAGCAACACCTACTCAACTCATT
TCATTTATTCACTCTTCAAAGAAGAAGGTAAACATTTATTCGATGTCCGTGAATCTATAC
TCGGTCATTTACAACAAGGTGGTACTCCTTCTGCAATTGATCGTATCTTTTCAACTCGTT
TAATGAATCATTATTATCAATTCTTGGAAAACGATCTCAAAGAACATGGTCATCTTCAAA
TGAATGGTTGTATTGGTTTCATTGATGGTGGTATTCATTACACTCCAATGCAAGAAATGA
TCGAA
Length of connected seq. 655
Full length Seq ID -
Full length Seq. -
Length of full length seq. -