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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHE481
Library VH
(Link to library)
Clone ID VHE481
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16495-1
Original site URL
Representative seq. ID VHE481P
(Link to Original site)
Representative DNA sequence 
>VHE481 (VHE481Q) /CSM/VH/VHE4-D/VHE481Q.Seq.d/
TGACAAAAGTTTATTCATTAGGTATTAGAAGAGAAGATAAGAATCGTTGGGAAAGAAGAG
CACCATTAGCGCCATCCCATGTTGAAGAATTAGTTAAAAAAGGTATTAAAGTTATTGTTC
AACCATCAACTCTTAGAAATTATCCAAATGTTTTATATGAGAAAGCAGGAGCAATCATTC
AAGAGGATTTAAAAGAGTGTGATGTAATTGTAGCAGTAAAGGAAGTACCAAGTGAATATT
TATACAATGATAAAACCTACATTTTCTTCTCACACACCATCAAAGCTCAACCATACAATA
TGGCAATGTTGGATGAAATTAACAAAAAACGTATTCGTTTAATCGATTATGAACGTATCA
CTGATGATCAAAATAGAAGATTAGTTCGTTTCGGTTCTTTTGCTGGTTATGCTGGTATGA
TTGATATGCTTCATGCATTAGGTGATCGTTTATTAGCAAAAGGTTTTTCAACACCATTCT
TGCATGTTGGTTATTCATATGTATATTCAAAACTTGAAAGTGCAATGGAAGCAGTTAAAG
CAATTGGTGAAGAGATTTCACAAGTTGGTTTACCAGATGACCTTTTACCATTTACTTTTG
CTTTTACTTCAGATGGTGCTGTATCACAAXXXXXXXXXXTTGTCATTAGAAGGTGTACCA
AATAGAGATTGTCTTCATCTTACCAAGACNTATGGTATTGAGAGTGTAAATACTCTTTTC
CGTGGTACCATCCGTTATAAAGGTTTCTGTCAAGTCATGGAAGCTGCCGTCGAAATTGGT
CTATTGGATACCACTAGCAAATCACATCTCGCCCCATCCGCCCCACCAATGTCATGGAAT
CTCGCAATGCGTCATATCTTACCAATTCCTCAAAGTGAAGGTATTCCAACTCAAGAACTT
TTAAGAAGAAAACTTAGAACTAGAAGAGGTTATCCTTCAAAAGGCTATGACGATGATAAG
ATTACCTATATCCTTTACGTCTTTGAGTGGTTAGATATCTTTAGTGAAAAGAATAATGTC
GCTCAATGTGGTAACTATTTCGAAGCTTTCACAGAGTTATTGAAAACTAAACTCAATCTC
TTACCATCAGAAAGAGATTTAATCATTTTACATAATGTCGTTGGTATCGAATGGGATGGT
GGTAAACATGAAACTAAAACTTCAACACTCGTTTACTATGGTTCAAAAGATCAATCTGCT
GTTAGCACTGTAGTTGGTTTACCAGTTGCTATCGCAGCTGAATTATTGGTTGAAAATCAA
ATTAAAGAAGTTGGTGCAATTAGACCAATCACTAAAGAATTTTATAAACCAATGTTAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
TKVYSLGIRREDKNRWERRAPLAPSHVEELVKKGIKVIVQPSTLRNYPNVLYEKAGAIIQ
EDLKECDVIVAVKEVPSEYLYNDKTYIFFSHTIKAQPYNMAMLDEINKKRIRLIDYERIT
DDQNRRLVRFGSFAGYAGMIDMLHALGDRLLAKGFSTPFLHVGYSYVYSKLESAMEAVKA
IGEEISQVGLPDDLLPFTFAFTSDGAVSQ---

---LSLEGVPNRDCLHLTKTYGIESVNTLFRGTIRYKGFCQVMEAAVEIGLLDTTSKSHL
APSAPPMSWNLAMRHILPIPQSEGIPTQELLRRKLRTRRGYPSKGYDDDKITYILYVFEW
LDIFSEKNNVAQCGNYFEAFTELLKTKLNLLPSERDLIILHNVVGIEWDGGKHETKTSTL
VYYGSKDQSAVSTVVGLPVAIAAELLVENQIKEVGAIRPITKEFYKPMLK
Translated Amino Acid sequence (All Frames) 
Frame A:
*qkfih*vleekirivgkeehh*rhpmlkn*lkkvlkllfnhqlleiiqmfymrkqeqsf
kri*ksvm*l*q*rkyqvniytmikptfsshtpsklnhtiwqcwmkltknvfv*simnvs
lmikied*fvsvlllvmlv*licfmh*vivy*qkvfqhhscmlvihmyiqnlkvqwkqlk
qlvkrfhklvyqmtfyhllllllqmvlyh---

---LSLEGVPNRDCLHLTKTYGIESVNTLFRGTIRYKGFCQVMEAAVEIGLLDTTSKSHL
APSAPPMSWNLAMRHILPIPQSEGIPTQELLRRKLRTRRGYPSKGYDDDKITYILYVFEW
LDIFSEKNNVAQCGNYFEAFTELLKTKLNLLPSERDLIILHNVVGIEWDGGKHETKTSTL
VYYGSKDQSAVSTVVGLPVAIAAELLVENQIKEVGAIRPITKEFYKPMLK

Frame B:
dkslfiry*krr*eslgkkstisaipc*ris*kry*sycstins*klskcfi*esrsnhs
rgfkrv*cncsskgstk*ifiq**nlhfllthhqsstiqygnvg*n*qktysfnrl*tyh
**sk*kissfrffcwlcwyd*yascir*sfiskrffntilacwlficifkt*kcngss*s
nw*rdftswftr*pftiyfcfyfrwccit---

---ch*kvyqieivfilprxmvlrv*ilfsvvpsvikvsvkswklpsklvywiplanhis
phpphqchgisqcvisyqflkvkvfqlknf*eenleleevilqkamtmirlpisftslsg
*islvkrimslnvvtisklsqsy*klnsisyhqkei*sfyimslvsngmvvnmklklqhs
ftmvqkinlllal*lvyqllsqlnywlkiklkklvqldqslknfinqc*

Frame C:
TKVYSLGIRREDKNRWERRAPLAPSHVEELVKKGIKVIVQPSTLRNYPNVLYEKAGAIIQ
EDLKECDVIVAVKEVPSEYLYNDKTYIFFSHTIKAQPYNMAMLDEINKKRIRLIDYERIT
DDQNRRLVRFGSFAGYAGMIDMLHALGDRLLAKGFSTPFLHVGYSYVYSKLESAMEAVKA
IGEEISQVGLPDDLLPFTFAFTSDGAVSQ---

---virrctk*rlsssyqdxwy*eckysfpwyhpl*rflsshgscrrnwsigyh*qitsr
pirptnvmesrnasyltnssk*rysnsrtfkkkt*n*krlsfkrl*r**dylyplrl*vv
ryl**ke*crsmw*lfrsfhrvien*tqsltirkrfnhft*crwyrmgww*t*n*nfntr
llwfkrsicc*hcswftscyrs*iig*ksn*rswcn*tnh*ril*tnvk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE481 (VHE481Q) /CSM/VH/VHE4-D/VHE481Q.Seq.d/                       2551   0.0
AHL713 (AHL713Q) /CSM/AH/AHL7-A/AHL713Q.Seq.d/                       1344   0.0
SHD787 (SHD787Q) /CSM/SH/SHD7-D/SHD787Q.Seq.d/                       1275   0.0
VHI192 (VHI192Q) /CSM/VH/VHI1-D/VHI192Q.Seq.d/                       1273   0.0
AFN810 (AFN810Q) /CSM/AF/AFN8-A/AFN810Q.Seq.d/                       1271   0.0
VHC756 (VHC756Q) /CSM/VH/VHC7-C/VHC756Q.Seq.d/                       1263   0.0
AHL574 (AHL574Q) /CSM/AH/AHL5-D/AHL574Q.Seq.d/                       1259   0.0
SHJ770 (SHJ770Q) /CSM/SH/SHJ7-C/SHJ770Q.Seq.d/                       1229   0.0
VHB321 (VHB321Q) /CSM/VH/VHB3-A/VHB321Q.Seq.d/                       1213   0.0
AHA214 (AHA214Q) /CSM/AH/AHA2-A/AHA214Q.Seq.d/                       1199   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

DY885658|DY885658.1 CeleSEQ1018 Cunninghamella elegans pBluescript (EcoRI-XhoI)Cunninghamella elegans cDNA clone CeleSEQ1018, mRNA sequence.    44   1e-07  3
EL573311|EL573311.1 Physarum03278 Physarum polycephalum starvation stress library Physarum polycephalum cDNA clone PpolyN1d53d12.t7, mRNA sequence.    52   2e-05  2
AC084621|AC084621.1 Caenorhabditis briggsae cosmid G44O14, complete sequence.    54   0.011  1
CB928143|CB928143.1 ABA1_36_A07.g1_A012 Abscisic acid-treated seedlings Sorghum bicolor cDNA clone ABA1_36_A07_A012 5', mRNA sequence.    42   0.046  3
AB066287|AB066287.1 Colwellia maris icl gene for isocitrate lyase, complete cds.    50   0.17   1
AC189034|AC189034.2 Gallus gallus BAC clone CH261-83E11 from chromosome z, complete sequence.    50   0.17   1
AC187592|AC187592.3 Gallus gallus BAC clone CH261-124L19 from chromosome z, complete sequence.    50   0.17   1
EJ803238|EJ803238.1 1093017442088 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061002761380 5', genomic survey sequence.    36   0.49   2
EH381154|EH381154.1 P20-52.4_H10_033.ab1 Marmoset hippocampus normalized cDNA library Callithrix jacchus cDNA, mRNA sequence.    48   0.69   1
CO648230|CO648230.1 ILLUMIGEN_MCQ_41537 Katze_MMPB2 Macaca mulatta cDNA clone IBIUW:23054 5' similar to Bases 5 to 948 highly similar to human RAB2B (Hs.22399), mRNA sequence.    48   0.69   1
dna update 2007. 8.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF038615_5(AF038615|pid:none) Caenorhabditis elegans cosmid R02D...   219   1e-55
AY751465_1(AY751465|pid:none) Oncorhynchus mykiss lysine ketoglu...   216   1e-54
BC073642_1(BC073642|pid:none) Xenopus laevis MGC82978 protein, m...   214   6e-54
(A2VCW9) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...   206   2e-51
(Q99K67) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...   205   3e-51
AK136216_1(AK136216|pid:none) Mus musculus in vitro fertilized e...   205   3e-51
AJ224761_1(AJ224761|pid:none) Mus musculus mRNA encoding lysine-...   205   3e-51
(A8E657) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...   202   2e-50
T02930(T02930)lysine-ketoglutarate reductase / saccharopine dehy...   193   1e-47
BT062000_1(BT062000|pid:none) Zea mays full-length cDNA clone ZM...   193   1e-47
protein update 2009. 6.30
PSORT 

 psg: 0.07  gvh: 0.19  alm: 0.28  top: 0.43  tms: 0.07  mit: 0.25  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.08  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 16.0 %: nuclear
	 16.0 %: vesicles of secretory system
	 12.0 %: endoplasmic reticulum
	  8.0 %: Golgi
	  8.0 %: mitochondrial
	  8.0 %: plasma membrane
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHE481 is cyt
5' end seq. ID VHE481F
5' end seq. 
>VHE481F.Seq
TGACAAAAGTTTATTCATTAGGTATTAGAAGAGAAGATAAGAATCGTTGGGAAAGAAGAG
CACCATTAGCGCCATCCCATGTTGAAGAATTAGTTAAAAAAGGTATTAAAGTTATTGTTC
AACCATCAACTCTTAGAAATTATCCAAATGTTTTATATGAGAAAGCAGGAGCAATCATTC
AAGAGGATTTAAAAGAGTGTGATGTAATTGTAGCAGTAAAGGAAGTACCAAGTGAATATT
TATACAATGATAAAACCTACATTTTCTTCTCACACACCATCAAAGCTCAACCATACAATA
TGGCAATGTTGGATGAAATTAACAAAAAACGTATTCGTTTAATCGATTATGAACGTATCA
CTGATGATCAAAATAGAAGATTAGTTCGTTTCGGTTCTTTTGCTGGTTATGCTGGTATGA
TTGATATGCTTCATGCATTAGGTGATCGTTTATTAGCAAAAGGTTTTTCAACACCATTCT
TGCATGTTGGTTATTCATATGTATATTCAAAACTTGAAAGTGCAATGGAAGCAGTTAAAG
CAATTGGTGAAGAGATTTCACAAGTTGGTTTACCAGATGACCTTTTACCATTTACTTTTG
CTTTTACTTCAGATGGTGCTGTATCACAANNNNNNNNNN
Length of 5' end seq. 639
3' end seq. ID VHE481Z
3' end seq. 
>VHE481Z.Seq
NNNNNNNNNNTTGTCATTAGAAGGTGTACCAAATAGAGATTGTCTTCATCTTACCAAGAC
NTATGGTATTGAGAGTGTAAATACTCTTTTCCGTGGTACCATCCGTTATAAAGGTTTCTG
TCAAGTCATGGAAGCTGCCGTCGAAATTGGTCTATTGGATACCACTAGCAAATCACATCT
CGCCCCATCCGCCCCACCAATGTCATGGAATCTCGCAATGCGTCATATCTTACCAATTCC
TCAAAGTGAAGGTATTCCAACTCAAGAACTTTTAAGAAGAAAACTTAGAACTAGAAGAGG
TTATCCTTCAAAAGGCTATGACGATGATAAGATTACCTATATCCTTTACGTCTTTGAGTG
GTTAGATATCTTTAGTGAAAAGAATAATGTCGCTCAATGTGGTAACTATTTCGAAGCTTT
CACAGAGTTATTGAAAACTAAACTCAATCTCTTACCATCAGAAAGAGATTTAATCATTTT
ACATAATGTCGTTGGTATCGAATGGGATGGTGGTAAACATGAAACTAAAACTTCAACACT
CGTTTACTATGGTTCAAAAGATCAATCTGCTGTTAGCACTGTAGTTGGTTTACCAGTTGC
TATCGCAGCTGAATTATTGGTTGAAAATCAAATTAAAGAAGTTGGTGCAATTAGACCAAT
CACTAAAGAATTTTATAAACCAATGTTAAAA
Length of 3' end seq. 691
Connected seq. ID VHE481P
Connected seq. 
>VHE481P.Seq
TGACAAAAGTTTATTCATTAGGTATTAGAAGAGAAGATAAGAATCGTTGGGAAAGAAGAG
CACCATTAGCGCCATCCCATGTTGAAGAATTAGTTAAAAAAGGTATTAAAGTTATTGTTC
AACCATCAACTCTTAGAAATTATCCAAATGTTTTATATGAGAAAGCAGGAGCAATCATTC
AAGAGGATTTAAAAGAGTGTGATGTAATTGTAGCAGTAAAGGAAGTACCAAGTGAATATT
TATACAATGATAAAACCTACATTTTCTTCTCACACACCATCAAAGCTCAACCATACAATA
TGGCAATGTTGGATGAAATTAACAAAAAACGTATTCGTTTAATCGATTATGAACGTATCA
CTGATGATCAAAATAGAAGATTAGTTCGTTTCGGTTCTTTTGCTGGTTATGCTGGTATGA
TTGATATGCTTCATGCATTAGGTGATCGTTTATTAGCAAAAGGTTTTTCAACACCATTCT
TGCATGTTGGTTATTCATATGTATATTCAAAACTTGAAAGTGCAATGGAAGCAGTTAAAG
CAATTGGTGAAGAGATTTCACAAGTTGGTTTACCAGATGACCTTTTACCATTTACTTTTG
CTTTTACTTCAGATGGTGCTGTATCACAA----------TTGTCATTAGAAGGTGTACCA
AATAGAGATTGTCTTCATCTTACCAAGACNTATGGTATTGAGAGTGTAAATACTCTTTTC
CGTGGTACCATCCGTTATAAAGGTTTCTGTCAAGTCATGGAAGCTGCCGTCGAAATTGGT
CTATTGGATACCACTAGCAAATCACATCTCGCCCCATCCGCCCCACCAATGTCATGGAAT
CTCGCAATGCGTCATATCTTACCAATTCCTCAAAGTGAAGGTATTCCAACTCAAGAACTT
TTAAGAAGAAAACTTAGAACTAGAAGAGGTTATCCTTCAAAAGGCTATGACGATGATAAG
ATTACCTATATCCTTTACGTCTTTGAGTGGTTAGATATCTTTAGTGAAAAGAATAATGTC
GCTCAATGTGGTAACTATTTCGAAGCTTTCACAGAGTTATTGAAAACTAAACTCAATCTC
TTACCATCAGAAAGAGATTTAATCATTTTACATAATGTCGTTGGTATCGAATGGGATGGT
GGTAAACATGAAACTAAAACTTCAACACTCGTTTACTATGGTTCAAAAGATCAATCTGCT
GTTAGCACTGTAGTTGGTTTACCAGTTGCTATCGCAGCTGAATTATTGGTTGAAAATCAA
ATTAAAGAAGTTGGTGCAATTAGACCAATCACTAAAGAATTTTATAAACCAATGTTAAAA
Length of connected seq. 1310
Full length Seq ID -
Full length Seq. -
Length of full length seq. -