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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF296
Library VH
(Link to library)
Clone ID VHF296
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U12449-1
Original site URL
Representative seq. ID VHF296P
(Link to Original site)
Representative DNA sequence 
>VHF296 (VHF296Q) /CSM/VH/VHF2-D/VHF296Q.Seq.d/
GATTATCAGAAAGATTACATTATGAAATTATACCAGATTGTATTAAAATTATTGAAAGCT
CAAAACAAAACAAAAAAGGTAACAACAACCATAACAACAATAATAATAATAATAGTAATA
AAGATTTAACATCTTTAATTGGTACATCATCAAATTCAGCATTTTCAAGTAATAGTACAA
GAACAGTTATTTCTCATAGTGGATCAGGTGAATTTTCAACTTATTCACCATXXXXXXXXX
XGATGAATTGGTTTGGAAACTCTTTAGTAAGATGGAAAATATTCAAGAGTTAAAACTTCN
TGCTCATTTCCATTTACCATCATTCATGGAATGGTTCGATGAAAACACCAACGTTAATAG
CGATGATATGATGATGATCAATTCAGTTTCGGATCAACCACTTTATAATCTTAAAANTCT
TGATTTAACAATGGCAGTTGTTTCATTACAAAGTATTCAACAAATTTTCCAACTTTGTCN
TCAACTTGAAAATTTCACTTTNTCAGCCGTTACNTTTGTTAATGATCAATCCATAGTTAT
TGATTGGTGTGCCGATGAATTAATCTTATTCTCATTCTTTGGTNTCTTTTCAAATTTAAG
AGAAATTAATTTATCAAATTCAAATATTGAACTTCGTAATATTAGAACTTTAATTTGTAA
AGGTCNTTCTCTTAGAATTATTCATTTANGGAATTGTAAAAATATTTCATCTC
sequence update 2002.10.25
Translated Amino Acid sequence 
LSERLHYEIIPDCIKIIESSKQNKKGNNNHNNNNNNNSNKDLTSLIGTSSNSAFSSNSTR
TVISHSGSGEFSTYSP---

---DELVWKLFSKMENIQELKLXAHFHLPSFMEWFDENTNVNSDDMMMINSVSDQPLYNL
KXLDLTMAVVSLQSIQQIFQLCXQLENFTXSAVTFVNDQSIVIDWCADELILFSFFGXFS
NLREINLSNSNIELRNIRTLICKGXSLRIIHLXNCKNISS
Translated Amino Acid sequence (All Frames) 
Frame A:
dyqkdyimklyqivlkllkaqnktkkvtttittiiiiiviki*hl*lvhhqiqhfqvivq
eqlflivdqvnfqlihh---

---DELVWKLFSKMENIQELKLXAHFHLPSFMEWFDENTNVNSDDMMMINSVSDQPLYNL
KXLDLTMAVVSLQSIQQIFQLCXQLENFTXSAVTFVNDQSIVIDWCADELILFSFFGXFS
NLREINLSNSNIELRNIRTLICKGXSLRIIHLXNCKNISS

Frame B:
iirkitl*nytrly*ny*klktkqkr*qqp*qq*******rfnifnwyiikfsifk**yk
nsyfs*wir*ifnlft---

---mnwfgnslvrwkifks*nfxlisiyhhswngsmktptliami***siqfrinhfiil
kxli*qwqlfhykvfnkfsnfvxnlkislxqplxllminp*lligvpmn*syshslvsfq
i*ekliyqiqilnfvilel*fvkvxllelfixgivkifhl

Frame C:
LSERLHYEIIPDCIKIIESSKQNKKGNNNHNNNNNNNSNKDLTSLIGTSSNSAFSSNSTR
TVISHSGSGEFSTYSP---

---*igletl**dgkysrvktscsfpftiihgmvr*khqr**r*ydddqfsfgsttl*s*
xs*fnngscfitkystnfptlsst*kfhfxsryxc**sihsy*lvcr*inlililwxlfk
fkrn*fikfky*ts*y*nfnl*rsfs*nysfxel*kyfi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF296 (VHF296Q) /CSM/VH/VHF2-D/VHF296Q.Seq.d/                       1066   0.0
VSH382 (VSH382Q) /CSM/VS/VSH3-D/VSH382Q.Seq.d/                        823   0.0
CHI537 (CHI537Q) /CSM/CH/CHI5-B/CHI537Q.Seq.d/                        733   0.0
SHK292 (SHK292Q) /CSM/SH/SHK2-D/SHK292Q.Seq.d/                         38   0.18
CHI376 (CHI376Q) /CSM/CH/CHI3-D/CHI376Q.Seq.d/                         38   0.18
VHM601 (VHM601Q) /CSM/VH/VHM6-A/VHM601Q.Seq.d/                         36   0.71
VFH289 (VFH289Q) /CSM/VF/VFH2-D/VFH289Q.Seq.d/                         36   0.71
SLA428 (SLA428Q) /CSM/SL/SLA4-B/SLA428Q.Seq.d/                         36   0.71
SHE203 (SHE203Q) /CSM/SH/SHE2-A/SHE203Q.Seq.d/                         36   0.71
VSE637 (VSE637Q) /CSM/VS/VSE6-B/VSE637Q.Seq.d/                         34   2.8
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439884) Dictyostelium discoideum cDNA clone:ddv41o24, 3' ...   886   0.0    1
(AU267363) Dictyostelium discoideum vegetative cDNA clone:VS...   821   0.0    1
(BJ380798) Dictyostelium discoideum cDNA clone:ddc39i09, 3' ...   731   0.0    1
(BJ421174) Dictyostelium discoideum cDNA clone:ddv41o24, 5' ...   216   3e-80  2
(AM479164) Vitis vinifera contig VV78X117658.4, whole genome...    56   0.002  1
(AE017263) Mesoplasma florum L1 complete genome.                   48   0.45   1
(AM437865) Vitis vinifera contig VV78X135777.7, whole genome...    34   0.48   3
(AC111670) Rattus norvegicus clone CH230-50J22, WORKING DRAF...    36   0.64   5
(AC193931) Pan troglodytes BAC clone CH251-176G20 from chrom...    46   1.8    1
(AC192289) Pan troglodytes BAC clone CH251-351P2 from chromo...    46   1.8    1
dna update 2008.10.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU031442_1(EU031442|pid:none) Arabidopsis thaliana ecotype Mt-0 ...    35   3.0
AB017065_14(AB017065|pid:none) Arabidopsis thaliana genomic DNA,...    35   3.0
EU031443_1(EU031443|pid:none) Arabidopsis thaliana ecotype Cvi-0...    34   5.1
protein update 2009. 3. 1
PSORT 

 psg: 0.75  gvh: 0.45  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VHF296 is cyt
5' end seq. ID VHF296F
5' end seq. 
>VHF296F.Seq
GATTATCAGAAAGATTACATTATGAAATTATACCAGATTGTATTAAAATTATTGAAAGCT
CAAAACAAAACAAAAAAGGTAACAACAACCATAACAACAATAATAATAATAATAGTAATA
AAGATTTAACATCTTTAATTGGTACATCATCAAATTCAGCATTTTCAAGTAATAGTACAA
GAACAGTTATTTCTCATAGTGGATCAGGTGAATTTTCAACTTATTCACCATNNNNNNNNN
N
Length of 5' end seq. 241
3' end seq. ID VHF296Z
3' end seq. 
>VHF296Z.Seq
NNNNNNNNNNGATGAATTGGTTTGGAAACTCTTTAGTAAGATGGAAAATATTCAAGAGTT
AAAACTTCNTGCTCATTTCCATTTACCATCATTCATGGAATGGTTCGATGAAAACACCAA
CGTTAATAGCGATGATATGATGATGATCAATTCAGTTTCGGATCAACCACTTTATAATCT
TAAAANTCTTGATTTAACAATGGCAGTTGTTTCATTACAAAGTATTCAACAAATTTTCCA
ACTTTGTCNTCAACTTGAAAATTTCACTTTNTCAGCCGTTACNTTTGTTAATGATCAATC
CATAGTTATTGATTGGTGTGCCGATGAATTAATCTTATTCTCATTCTTTGGTNTCTTTTC
AAATTTAAGAGAAATTAATTTATCAAATTCAAATATTGAACTTCGTAATATTAGAACTTT
AATTTGTAAAGGTCNTTCTCTTAGAATTATTCATTTANGGAATTGTAAAAATATTTCATC
TC
Length of 3' end seq. 482
Connected seq. ID VHF296P
Connected seq. 
>VHF296P.Seq
GATTATCAGAAAGATTACATTATGAAATTATACCAGATTGTATTAAAATTATTGAAAGCT
CAAAACAAAACAAAAAAGGTAACAACAACCATAACAACAATAATAATAATAATAGTAATA
AAGATTTAACATCTTTAATTGGTACATCATCAAATTCAGCATTTTCAAGTAATAGTACAA
GAACAGTTATTTCTCATAGTGGATCAGGTGAATTTTCAACTTATTCACCAT---------
-GATGAATTGGTTTGGAAACTCTTTAGTAAGATGGAAAATATTCAAGAGTTAAAACTTCN
TGCTCATTTCCATTTACCATCATTCATGGAATGGTTCGATGAAAACACCAACGTTAATAG
CGATGATATGATGATGATCAATTCAGTTTCGGATCAACCACTTTATAATCTTAAAANTCT
TGATTTAACAATGGCAGTTGTTTCATTACAAAGTATTCAACAAATTTTCCAACTTTGTCN
TCAACTTGAAAATTTCACTTTNTCAGCCGTTACNTTTGTTAATGATCAATCCATAGTTAT
TGATTGGTGTGCCGATGAATTAATCTTATTCTCATTCTTTGGTNTCTTTTCAAATTTAAG
AGAAATTAATTTATCAAATTCAAATATTGAACTTCGTAATATTAGAACTTTAATTTGTAA
AGGTCNTTCTCTTAGAATTATTCATTTANGGAATTGTAAAAATATTTCATCTC
Length of connected seq. 703
Full length Seq ID -
Full length Seq. -
Length of full length seq. -