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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF693
Library VH
(Link to library)
Clone ID VHF693
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHF693P
(Link to Original site)
Representative DNA sequence 
>VHF693 (VHF693Q) /CSM/VH/VHF6-D/VHF693Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GNGCCATTATGGACCGTCGNGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGANCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGATCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAAGTCACGTTGATTTCA
XXXXXXXXXXGATGAATTAACTGCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGA
AATTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTANTCAATCGTCGTAGAGG
TATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTT
ACCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGC
TTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAA
AGCCNCTGAAGTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGA
TCTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRXGIIADKV
SGDMRYMSCRADEQDRGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPSHVDF-
--

---DELTASPXILEPIYLVEITAPENAIGGIXSVXNRRRGIVIGEERRIGSPLLSVKAHL
PVLESFGFTDDLRSHTAGQAFPQCVFDHWASIGVVNKDKKAXEVALAXRKRKGLAPEIPD
LTNSTKNSKQFKSHLSFQ
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfxplwtvxkiseicllllmsitvkplyqil*skxlvllpik
flvi*dichvvlmnkivvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
---

---DELTASPXILEPIYLVEITAPENAIGGIXSVXNRRRGIVIGEERRIGSPLLSVKAHL
PVLESFGFTDDLRSHTAGQAFPQCVFDHWASIGVVNKDKKAXEVALAXRKRKGLAPEIPD
LTNSTKNSKQFKSHLSFQ

Frame B:
tleckkskvqng*fhnrsnsxhygpsxkyqkyvcycscrsr*nhfirffnpkxwyycr*s
fw*yeiyvmsc**trswyhh*ificitslrnakgrqitswlyftrifnqsyrfpksr*f-
--

---mn*lppqxs*nqst**kllpqktllvvfxlxsivvevlllvknvvsvphyylskliy
qy*nxsvspmiyvptlpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqi
*qiprktlnnlsrt*vf

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRXGIIADKV
SGDMRYMSCRADEQDRGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPSHVDF-
--

---*inclpxylrtnllsrnycprkrywwylxcxqss*rycyw*rtsyrfpiiicqssft
sirixrfhr*ftfphcrssfptmclrslgfnwccqqr*esx*srfsxqkekrfsprnprs
dkfhekl*ti*valefs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF693 (VHF693Q) /CSM/VH/VHF6-D/VHF693Q.Seq.d/                       1384   0.0
VHF686 (VHF686Q) /CSM/VH/VHF6-D/VHF686Q.Seq.d/                        767   0.0
VHF489 (VHF489Q) /CSM/VH/VHF4-D/VHF489Q.Seq.d/                        767   0.0
VHF330 (VHF330Q) /CSM/VH/VHF3-B/VHF330Q.Seq.d/                        763   0.0
VHE802 (VHE802Q) /CSM/VH/VHE8-A/VHE802Q.Seq.d/                        763   0.0
VHF836 (VHF836Q) /CSM/VH/VHF8-B/VHF836Q.Seq.d/                        757   0.0
VHF458 (VHF458Q) /CSM/VH/VHF4-C/VHF458Q.Seq.d/                        757   0.0
VHF473 (VHF473Q) /CSM/VH/VHF4-D/VHF473Q.Seq.d/                        755   0.0
VHF772 (VHF772Q) /CSM/VH/VHF7-C/VHF772Q.Seq.d/                        753   0.0
VHF660 (VHF660Q) /CSM/VH/VHF6-C/VHF660Q.Seq.d/                        753   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(M26017) D.discoideum elongation factor 2 mRNA, complete cds.     638   0.0    4
(BJ440179) Dictyostelium discoideum cDNA clone:ddv42k22, 3' ...   765   0.0    1
(BJ440172) Dictyostelium discoideum cDNA clone:ddv42i24, 3' ...   765   0.0    1
(BJ439833) Dictyostelium discoideum cDNA clone:ddv41b24, 3' ...   765   0.0    1
(BJ439727) Dictyostelium discoideum cDNA clone:ddv41l07, 3' ...   763   0.0    1
(BJ439302) Dictyostelium discoideum cDNA clone:ddv40d02, 3' ...   763   0.0    1
(BJ440005) Dictyostelium discoideum cDNA clone:ddv42h10, 3' ...   757   0.0    1
(BJ439763) Dictyostelium discoideum cDNA clone:ddv41d16, 3' ...   757   0.0    1
(BJ439830) Dictyostelium discoideum cDNA clone:ddv41b20, 3' ...   755   0.0    1
(BJ440066) Dictyostelium discoideum cDNA clone:ddv42d16, 3' ...   749   0.0    1
dna update 2008.10.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK319019_1(AK319019|pid:none) Arabidopsis thaliana AT1G56070 mRN...    88   1e-33
AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   142   9e-33
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   139   8e-32
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   138   2e-31
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   137   4e-31
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   137   5e-31
AY144924_1(AY144924|pid:none) Saccharomyces castellii clone Cont...   133   5e-30
CP000881_15(CP000881|pid:none) Hemiselmis andersenii chromosome ...   133   5e-30
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   133   7e-30
AP005845_16(AP005845|pid:none) Oryza sativa Japonica Group genom...   132   2e-29
protein update 2009. 7. 4
PSORT 

 psg: 0.71  gvh: 0.37  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VHF693 is cyt
5' end seq. ID VHF693F
5' end seq. 
>VHF693F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GNGCCATTATGGACCGTCGNGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGANCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGATCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAAGTCACGTTGATTTCA
NNNNNNNNNN
Length of 5' end seq. 370
3' end seq. ID VHF693Z
3' end seq. 
>VHF693Z.Seq
NNNNNNNNNNGATGAATTAACTGCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGA
AATTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTANTCAATCGTCGTAGAGG
TATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTT
ACCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGC
TTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAA
AGCCNCTGAAGTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGA
TCTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAA
Length of 3' end seq. 415
Connected seq. ID VHF693P
Connected seq. 
>VHF693P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GNGCCATTATGGACCGTCGNGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGANCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGATCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAAGTCACGTTGATTTCA
----------GATGAATTAACTGCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGA
AATTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTANTCAATCGTCGTAGAGG
TATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTT
ACCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGC
TTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAA
AGCCNCTGAAGTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGA
TCTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAA
Length of connected seq. 765
Full length Seq ID -
Full length Seq. -
Length of full length seq. -